小尾寒羊FSHR基因在生殖轴的表达研究
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摘要
为了揭示FSHR基因在小尾寒羊下丘脑-垂体-卵巢轴(Hypothalamic-pituitary-ovarian axis,HPOA)中的表达规律,深入了解其对小尾寒羊多羔的作用,采用实时荧光定量PCR技术对6只小尾寒羊(FecB++型单、多羔母羊各3只)的生殖组织及脑组织中FSHR基因的表达谱进行分析。结果显示:FSHR基因在大脑、卵巢和垂体组织表达,其中在卵巢高表达;FSHR基因在小尾寒羊多羔群体卵巢和垂体的表达量均极显著高于单羔群体(P<0.01)。研究发现,FSHR基因的表达水平与小尾寒羊产羔数之间存在一定程度相关,暗示其可能参与了小尾寒羊多羔性状调控。
In order to reveal the expression patterns of FSHR gene in the hypothalamic-pituitary-ovarian axis(HPOA)in Small-tail han sheep,and further research the effect of FSHR gene in prolificacy,the real-time fluorescence quantitative PCR(RT-q PCR)method was used to detect the expression of the FSHR gene in reproductive tissues and brain tissues of six Small-tail han sheep(three polytocous and three monotocous ewes from the FecB++ genotype)in this study. The results showed that FSHR gene expressed in the brain,ovary and pituitary tissues,FSHR was highly expressed in ovary. The expression of FSHR gene in ovary and pituitary of polytocous Small-tail han ewes was extremely significantly higher(P<0.01)than that of monotocous ewes. The preliminaryresults ofthis study showed that there was a positive correlation between the expression level of FSHR gene and the litter size of Small-tail han sheep,so,the FSHR gene maybe involve in the regulation ofmultiple lambs in Small-tail han sheep.
In order to reveal the expression patterns of FSHR gene in the hypothalamic-pituitary-ovarian axis(HPOA)in Small-tail han sheep,and further research the effect of FSHR gene in prolificacy,the real-time fluorescence quantitative PCR(RT-q PCR)method was used to detect the expression of the FSHR gene in reproductive tissues and brain tissues of six Small-tail han sheep(three polytocous and three monotocous ewes from the FecB++ genotype)in this study. The results showed that FSHR gene expressed in the brain,ovary and pituitary tissues,FSHR was highly expressed in ovary. The expression of FSHR gene in ovary and pituitary of polytocous Small-tail han ewes was extremely significantly higher(P<0.01)than that of monotocous ewes. The preliminaryresults ofthis study showed that there was a positive correlation between the expression level of FSHR gene and the litter size of Small-tail han sheep,so,the FSHR gene maybe involve in the regulation ofmultiple lambs in Small-tail han sheep.
引文
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[11]龙威海,丁玫,冯文武,等.卵泡刺激激素受体基因(FSHR)在贵州地方山羊的多态性及表达研究[J].农业生物技术学报,2015,23(6):755-761.
[12]Huo S,Chen S,Long R,et al.Protein and m RNA expression of follicle-stimulating hormone receptor and luteinizing hormone receptor during the oestrus in the yak(Bos grunniens)[J].Reproduction in Domestic Animals,2017,52(3):477-482.
[13]Chen H,Cui Y,Yu S.Expression and localization of FSHR,GHR and LHR in different tissues and reproductive organs of female yaks[J].Folia Morphologica,2017,77(2):301-309.
[14]Goyal S,Aggarwal J,Dubey P K,et al.Expression analysis of genes associated with prolificacy in Fec B carrier and noncarrier Indian sheep[J].Animal Biotechnology,2017,28(3):220-227.
[2]王建英,曹顶国,刘昭华,等.绵羊多羔性状候选基因的研究进展[J].草食家畜,2017(3):1-8.
[3]鲁绍雄,吴常信.动物遗传标记辅助选择研究及其应用[J].遗传,2002,24(3):359-362.
[4]吴阳升,林嘉鹏,蒋香菊,等.绵羊FSHR基因可变剪接体的克隆、鉴定及表达分析[J].江苏农业学报,2017,33(3):630-637.
[5]Simoni M,Gromoll J,Nieschlag E.The follicle-stimulating hormone receptor:biochemistry,molecular biology,physiology,and pathophysiology[J].Endocrine Reviews,1997,18(6):739-773.
[6]朱吉,杨仕柳,欧阳叙向,等.山羊FSHR基因5′端多态性与产仔数性状的关系[J].草食家畜,2007(1):12-16.
[7]胡亮,字向东,卢建远,等.多胎和单胎山羊品种促性腺素基因及其受体基因的克隆、序列分析及组织表达研究[J].西南民族大学学报(自然科学版),2015,41(2):138-143.
[8]Liu Lingbin,Li Diyan,Gilbert ER,et al.Effects of monochromatic light on expression of estrogen receptor(ER)and progesterone receptor(PR)in ovarian follicles of chicken[J].PLo S ONE,2015,10:1-14.
[9]Scarlet D,Walter I,Hlavaty J,et al.Expression and immunolocalisation of follicle-stimulating hormone receptors in gonads of newborn and adult female horses[J].Reproduction,Fertility and Development,2016,28(9):1340-1348.
[10]Mehl N S,Khalid M,Srisuwatanasagul S,et al.Comparison of the ovarian and uterine reproductive parameters,and the ovarian m RNAand protein expression of LHR and FSHR between the prepubertal and adult female cats[J].Reproduction in Domestic Animals,2017,52(S2):41-44.
[11]龙威海,丁玫,冯文武,等.卵泡刺激激素受体基因(FSHR)在贵州地方山羊的多态性及表达研究[J].农业生物技术学报,2015,23(6):755-761.
[12]Huo S,Chen S,Long R,et al.Protein and m RNA expression of follicle-stimulating hormone receptor and luteinizing hormone receptor during the oestrus in the yak(Bos grunniens)[J].Reproduction in Domestic Animals,2017,52(3):477-482.
[13]Chen H,Cui Y,Yu S.Expression and localization of FSHR,GHR and LHR in different tissues and reproductive organs of female yaks[J].Folia Morphologica,2017,77(2):301-309.
[14]Goyal S,Aggarwal J,Dubey P K,et al.Expression analysis of genes associated with prolificacy in Fec B carrier and noncarrier Indian sheep[J].Animal Biotechnology,2017,28(3):220-227.