HPLC法测定对乙酰氨基酚片的含量
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摘要
目的建立HPLC法测定对乙酰氨基酚片的含量。方法色谱柱为Agilent Eclipse XDB-C18柱(4.6 mm×150 mm,5μm);流动相为甲醇-水(20∶80),流速为1.0 ml/min,检测波长为244 nm,柱温30℃。结果对乙酰氨基酚浓度在10.16～30.48μg/ml范围内线性关系良好,r=0.9998(n=5);平均加样回收率为100.04%,RSD=0.95%(n=6)。结论 HPLC法操作简便、结果准确、重复性好、专属性强,适用于测定对乙酰氨基酚片中对乙酰氨基酚的含量。
Objective To establish a HPLC method for the determination of acetaminophen tablets.Methods The column was an Agilent Eclipse XDB-C18 column(4.6 mm×150 mm,5μm);the mobile phase was methanol-water(20:80),the flow rate was 1.0 ml/min,the detection wavelength was 244 nm,and the column temperature was 30° C.Results The linear relationship of acetaminophen concentration in the range of 10.16 ～30.48μg/ml was good,r =0.9998(n =5);the average recovery was 100.04%,RSD =0.95%(n =6).Conclusion The HPLC method is simple,accurate,reproducible and specific.It is suitable for the determination of acetaminophen in acetaminophen tablets.
Objective To establish a HPLC method for the determination of acetaminophen tablets.Methods The column was an Agilent Eclipse XDB-C18 column(4.6 mm×150 mm,5μm);the mobile phase was methanol-water(20:80),the flow rate was 1.0 ml/min,the detection wavelength was 244 nm,and the column temperature was 30° C.Results The linear relationship of acetaminophen concentration in the range of 10.16 ～30.48μg/ml was good,r =0.9998(n =5);the average recovery was 100.04%,RSD =0.95%(n =6).Conclusion The HPLC method is simple,accurate,reproducible and specific.It is suitable for the determination of acetaminophen in acetaminophen tablets.
引文
[1]国家药典委员会.中国药典.二部[S].北京:中国医药科技出版社,2015:319.
[2]石向群,方莉,徐景秀,等.液相色谱法测定对乙酰氨基酚片的含量[J].广州化工,2012,40(1):93-97.
[3]逯小萌.反相高效液相色谱法测定对乙酰氨基酚片的含量[J].海峡药学,2016,28(10):56-58.
[4]董超琪.HPLC法测定对乙酰氨基酚滴剂的含量[J].海峡药学,2014,26(5):52-54.
[5]胡俊,袁常,珍廖晴,等.高效液相色谱法测定氨咖黄敏胶囊中对乙酰氨基酚含量[J].泸州医学院学报,2016,39(02):158-161.
[2]石向群,方莉,徐景秀,等.液相色谱法测定对乙酰氨基酚片的含量[J].广州化工,2012,40(1):93-97.
[3]逯小萌.反相高效液相色谱法测定对乙酰氨基酚片的含量[J].海峡药学,2016,28(10):56-58.
[4]董超琪.HPLC法测定对乙酰氨基酚滴剂的含量[J].海峡药学,2014,26(5):52-54.
[5]胡俊,袁常,珍廖晴,等.高效液相色谱法测定氨咖黄敏胶囊中对乙酰氨基酚含量[J].泸州医学院学报,2016,39(02):158-161.