激光联合射频导入氨甲环酸注射液对黄褐斑动物模型的研究
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摘要
目的:通过黄褐斑动物实验模型,研究Q开关Nd:YAG 1064nm激光联合射频导入氨甲环酸治疗黄褐斑的疗效,为临床实践作指导。方法:将55只豚鼠分为正常对照组及模型组。正常对照组10只,模型组为将造模成功的豚鼠45只平均分为三组:A组为单用Q开关激光治疗组; B组为单用射频导入氨甲环酸注射液治疗组; C组为Q开关Nd:YAG 1064nm激光+射频导入氨甲环酸注射液治疗组。治疗结束后,分别取正常豚鼠背部皮肤、造模后背部皮肤、治疗后即刻、治疗后1wk、治疗后3wk的小鼠背部皮肤,分别行HE染色、MelanA免疫组化染色观察黑素颗粒的变化情况。采用图像分析法分析治疗前后表皮黑色素颗粒的改变。结果:造模成功的豚鼠背部皮损经过治疗后黑素颗粒面积,黑素细胞数量均随时间推移而有所下降,平均灰度值有所上升,差异具有统计学意义(P≤0.05); C组与A、B组数据有统计学差异,A与B组数据无统计学差异。说明联合使用Q开关Nd:YAG 1064nm激光和射频导入氨甲环酸注射液与单独使用Q开关Nd:YAG 1064nm激光或单独使用射频导入氨甲环酸注射液相比其结果有统计学差异,而单独使用Q开关Nd:YAG激光与单独使用射频导入氨甲环酸注射液其结果无统计学差异。结论:联合应用Q开关Nd:YAG 1064nm激光及射频导入氨甲环酸注射液治疗黄褐斑的有一定的治疗效果,且联合应用优于其单独应用。
Objective:To guide clinical practice,we investigate the curative effect of Q Switched1064 nmLaser Combined with radio frequency importing tranexamic acid injection in the treatment of melanocytes in chloasma animal model.Methods:55 Guinea pigs were divided into normal group(10)and model group(45).Randomly divided into A,B and C group on average.A group treated by Q Switched 1064 nmLaser,B group treated by radio frequency importing tranexamic acid injection,C group treated by Q Switched 1064 nmLaser combined with radio frequency importing tranexamic acid injection.The guinea pig back skins of Normal group and model group were taken after establishing model group,taking therapies,taking 1 week therapies and taking 3 weeks therapies.The back skins were treated with H&E stain and Immuohistochemical staining through which changes of melanin granules in the back skins were observed.Image analysis was used to analyze the changes of epidermal melanin granules in the back skins.Results:Compared with the normal group,significant increase was observed in the changes of melanin granules in the guinea pig back skin of the model group.Compared to the untreated right skin lesions of control group,left skin lesions were treated with the therapies above.The area of melanin granules,the number of melanocytes all decreased over time,while the average gray values increased.The difference was statistically significant(P ≤ 0.05).Group C showed significant difference with group Band group A,while Group A and Group B showed no significant difference.Conclusion:The group treated with Q Switched 1064 nmLaser combined with radio frequency importing tranexamic acid injection shows some therapeutic effects in the treatment of Guinea pig model of melisma.The efficacy of combined application is superior to its individual application.
Objective:To guide clinical practice,we investigate the curative effect of Q Switched1064 nmLaser Combined with radio frequency importing tranexamic acid injection in the treatment of melanocytes in chloasma animal model.Methods:55 Guinea pigs were divided into normal group(10)and model group(45).Randomly divided into A,B and C group on average.A group treated by Q Switched 1064 nmLaser,B group treated by radio frequency importing tranexamic acid injection,C group treated by Q Switched 1064 nmLaser combined with radio frequency importing tranexamic acid injection.The guinea pig back skins of Normal group and model group were taken after establishing model group,taking therapies,taking 1 week therapies and taking 3 weeks therapies.The back skins were treated with H&E stain and Immuohistochemical staining through which changes of melanin granules in the back skins were observed.Image analysis was used to analyze the changes of epidermal melanin granules in the back skins.Results:Compared with the normal group,significant increase was observed in the changes of melanin granules in the guinea pig back skin of the model group.Compared to the untreated right skin lesions of control group,left skin lesions were treated with the therapies above.The area of melanin granules,the number of melanocytes all decreased over time,while the average gray values increased.The difference was statistically significant(P ≤ 0.05).Group C showed significant difference with group Band group A,while Group A and Group B showed no significant difference.Conclusion:The group treated with Q Switched 1064 nmLaser combined with radio frequency importing tranexamic acid injection shows some therapeutic effects in the treatment of Guinea pig model of melisma.The efficacy of combined application is superior to its individual application.
引文
[1]Werlinger KD,Guevara IL,González CM,et al.Prevalence of self-diagnosed melasma among premenopausal latino women in dallas and fort worth[J].Tex.Arch Dermatol,2007;143(3):424-5
[2]Kang HY,Bahadoran P,Suzuki I,et al.In vivo reflectance confocal microscopy detects pigmentary changes in melasma at a cellular level resolution[J].Exp Dermatol,2010;19(8):e 228-33
[3]梁颖.黄褐斑药物治疗的研究进展[J].天津药学,2010;22(6):63-66
[4]张宁,陈巧云,任燕冬,等.中药治疗黄褐斑实验研究进展[J].中国美容医学,2008;(6):940-943
[5]Sim JH,Park YL,Lee JS,et al.Treatment of melasma by low-fluence 1064nm Q-switched Nd:yag laser[J].J Dermatolog Treat,2014;25(3):212-7
[6]Tourlaki A,Galimberti MG,Pellacani G,et al.Combination of fractional erbium-glass laser and topical therapy in melasma resistant to triple-combination cream[J].J Dermatolog Treat,2014;25(3):218-22
[7]周海林,胡白,张弛.694nm点阵红宝石激光联合左旋维生素C超声导入治疗黄褐斑的疗效观察[J].中国激光医学杂志,2016;25(5):289
[8]孙志平,江浩波,张淑芳,等.超声导入氨甲环酸联合谷胱甘肽治疗黄褐斑的疗效观察[J].皮肤性病诊疗学杂志,2015;22(1):53-55
[9]张俊,胡飞虎,孙彩虹.维生素C超声导入联合氢醌乳膏治疗黄褐斑疗效观察[J].国际皮肤性病学杂志,2014;40(3):199-200
[10]张昆梅,张莉.超声导入复方维A酸霜加外敷美白面膜治疗黄褐斑35例[J].中国麻风皮肤病杂志,2006;(6):518
[11]Li D,Shi Y,Li M,et al.Tranexamic acid can treat ultraviolet radiation-induced pigmentation in guinea pigs[J].Eur J Dermatol,2010;20(3):289-92
[12]安彩霞,向芳,杨珊,等.氨甲环酸联合Q开关Nd:yag激光治疗黄褐斑疗效观察[J].实用皮肤病学杂志,2015;(2):126-128
[13]赵欣欣,李平,李燕,等.口服氨甲环酸联合Q开关Nd:yag激光治疗黄褐斑疗效观察[J].中国美容医学,2016;25(12):69-71
[14]中华中医药学会中药实验药理专业委员会[J].中药新药与临床药理,2017;28(5):649-653
[15]戴岳,曹亮,陈解春,等.积雪苷霜软膏对豚鼠皮肤色素沉着的预防作用[J].世界临床药物,2007;(8):464-468+492
[16]殷悦,李潼,樊星,等.黄褐斑的治疗现状[J].中国美容整形外科杂志,2017;28(7):446-449
[17]Rusciani A,Motta A,Rusciani L,et al.Q-switched alexandrite laser-assisted treatment of melasma:2-year follow-up monitoring[J].J Drugs Dermatol,2005;4(6):770-4
[18]Kim HS,Kim EK,Jung KE,et al.A split-face comparison of low-fluence Q-switched Nd:yag laser plus 1550nm fractional photothermolysis vs.Q-switched Nd:yag monotherapy for facial melasma in asian skin[J].J Cosmet Laser Ther,2013;15(3):143-9
[19]Kang HY,Ortonne JP.What should be considered in treatment of melasma[J].Ann Dermatol,2010;22(4):373-8
[20]Cho HH,Choi M,Cho S,et al.Role of oral tranexamic acid in melasma patients treated with ipl and low fluence QS Nd:yag laser[J].J Dermatolog Treat,2013;24(4):292-6
[21]杨宝峰.药理学[M].第7版.北京.人民卫生出版社,2008:289
[22]二條贞子.トラネキサム酸による肝斑の治療[J].基礎と臨床,1979;13(9):3129-3131
[23]Zhou LL,Baibergenova A.Melasma:systematic review of the systemic treatments[J].Int J Dermatol,2017;56(9):902-908
[24]王强,胡伟,徐芳辉,等.氨甲环酸:一种治疗黄褐斑的重要辅助药物[J].中南药学,2014;12(9):889-895
[25]Li Y,Sun Q,He Z,et al.Treatment of melasma with oral administration of compoundtranexamic acid:a preliminary clinical trial[J].Eur Acad Dermatol Venereol,2014;28(3):393-4
[26]韩雪,曹莫.氨甲环酸治疗黄褐斑的研究现状与用药安全分析[J].中国医疗美容,2014;4(2):90-91
[27]田利民,王晨霞,孙立.射频导入参棘软膏联合口服氨甲环酸、谷胱甘肽治疗黄褐斑疗效观察[J].内蒙古医科大学学报,2017;39(6):499-502+507
[2]Kang HY,Bahadoran P,Suzuki I,et al.In vivo reflectance confocal microscopy detects pigmentary changes in melasma at a cellular level resolution[J].Exp Dermatol,2010;19(8):e 228-33
[3]梁颖.黄褐斑药物治疗的研究进展[J].天津药学,2010;22(6):63-66
[4]张宁,陈巧云,任燕冬,等.中药治疗黄褐斑实验研究进展[J].中国美容医学,2008;(6):940-943
[5]Sim JH,Park YL,Lee JS,et al.Treatment of melasma by low-fluence 1064nm Q-switched Nd:yag laser[J].J Dermatolog Treat,2014;25(3):212-7
[6]Tourlaki A,Galimberti MG,Pellacani G,et al.Combination of fractional erbium-glass laser and topical therapy in melasma resistant to triple-combination cream[J].J Dermatolog Treat,2014;25(3):218-22
[7]周海林,胡白,张弛.694nm点阵红宝石激光联合左旋维生素C超声导入治疗黄褐斑的疗效观察[J].中国激光医学杂志,2016;25(5):289
[8]孙志平,江浩波,张淑芳,等.超声导入氨甲环酸联合谷胱甘肽治疗黄褐斑的疗效观察[J].皮肤性病诊疗学杂志,2015;22(1):53-55
[9]张俊,胡飞虎,孙彩虹.维生素C超声导入联合氢醌乳膏治疗黄褐斑疗效观察[J].国际皮肤性病学杂志,2014;40(3):199-200
[10]张昆梅,张莉.超声导入复方维A酸霜加外敷美白面膜治疗黄褐斑35例[J].中国麻风皮肤病杂志,2006;(6):518
[11]Li D,Shi Y,Li M,et al.Tranexamic acid can treat ultraviolet radiation-induced pigmentation in guinea pigs[J].Eur J Dermatol,2010;20(3):289-92
[12]安彩霞,向芳,杨珊,等.氨甲环酸联合Q开关Nd:yag激光治疗黄褐斑疗效观察[J].实用皮肤病学杂志,2015;(2):126-128
[13]赵欣欣,李平,李燕,等.口服氨甲环酸联合Q开关Nd:yag激光治疗黄褐斑疗效观察[J].中国美容医学,2016;25(12):69-71
[14]中华中医药学会中药实验药理专业委员会[J].中药新药与临床药理,2017;28(5):649-653
[15]戴岳,曹亮,陈解春,等.积雪苷霜软膏对豚鼠皮肤色素沉着的预防作用[J].世界临床药物,2007;(8):464-468+492
[16]殷悦,李潼,樊星,等.黄褐斑的治疗现状[J].中国美容整形外科杂志,2017;28(7):446-449
[17]Rusciani A,Motta A,Rusciani L,et al.Q-switched alexandrite laser-assisted treatment of melasma:2-year follow-up monitoring[J].J Drugs Dermatol,2005;4(6):770-4
[18]Kim HS,Kim EK,Jung KE,et al.A split-face comparison of low-fluence Q-switched Nd:yag laser plus 1550nm fractional photothermolysis vs.Q-switched Nd:yag monotherapy for facial melasma in asian skin[J].J Cosmet Laser Ther,2013;15(3):143-9
[19]Kang HY,Ortonne JP.What should be considered in treatment of melasma[J].Ann Dermatol,2010;22(4):373-8
[20]Cho HH,Choi M,Cho S,et al.Role of oral tranexamic acid in melasma patients treated with ipl and low fluence QS Nd:yag laser[J].J Dermatolog Treat,2013;24(4):292-6
[21]杨宝峰.药理学[M].第7版.北京.人民卫生出版社,2008:289
[22]二條贞子.トラネキサム酸による肝斑の治療[J].基礎と臨床,1979;13(9):3129-3131
[23]Zhou LL,Baibergenova A.Melasma:systematic review of the systemic treatments[J].Int J Dermatol,2017;56(9):902-908
[24]王强,胡伟,徐芳辉,等.氨甲环酸:一种治疗黄褐斑的重要辅助药物[J].中南药学,2014;12(9):889-895
[25]Li Y,Sun Q,He Z,et al.Treatment of melasma with oral administration of compoundtranexamic acid:a preliminary clinical trial[J].Eur Acad Dermatol Venereol,2014;28(3):393-4
[26]韩雪,曹莫.氨甲环酸治疗黄褐斑的研究现状与用药安全分析[J].中国医疗美容,2014;4(2):90-91
[27]田利民,王晨霞,孙立.射频导入参棘软膏联合口服氨甲环酸、谷胱甘肽治疗黄褐斑疗效观察[J].内蒙古医科大学学报,2017;39(6):499-502+507