棉花导入系耐盐性鉴定及耐盐基因QTL定位
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摘要
【目的】筛选棉花耐盐、抗盐种质。【方法】以148份陆地棉背景的海岛棉染色体片断导入系为研究对象,利用苗期植株相对生长量进行耐盐性鉴定。【结果】148个导入系材料的平均盐害系数为61.22%,耐盐性差异较大;以2个亲本的盐害系数为界线,将盐害系数分成3组,盐害系数0~29.35%的占23.65%,29.35%~71.70%的占39.19%,71.70%~100%的占37.16%。【结论】通过对耐盐性状的QTL(Quantitative trait locus)定位,检测到了23个耐盐相关的QTL,其中12个耐盐QTL分布在A05、A12、D05和D11染色体上,说明从陆地棉背景的海岛棉染色体片段导入系中进行耐盐材料筛选是可行的,对特定染色体的筛选会具有更高的效率。
[Objective] Tolerance evaluation is the basis for cotton salinity-resistant breeding.[Method] Relative growth of cotton plants at seedling stage for 148 introgression lines was used to evaluate the tolerance to salinity.[Result] The results demonstrated that salt damage coefficients were significantly different from each other,with an average 61.22% ranging from1.34% to 100%.Based on the salt damage coefficient,148 introgression lines were divided into three groups;group 1(23.65%) was more tolerant than the tolerant parent with the salt damage coefficient less than 29.35%;group 2(39.19%)was more sensitive than the tolerant parent with the salt damage coefficient from 29.35% ―71.70%;group 3(37.16%) was more sensitive than the susceptible parent with the salt damage coefficient above 71.70%.[Condusion] Mapping of QTLs(Quantitative trait locus) for salt-tolerant traits indicated that 23 QTLs for tolerance-related traits were detected.Among them,12 QTLs were aggregately distributed on chromosomes A05,A12,D05 and D11.Our results demonstrate that it is feasible for screening salt-tolerant introgression lines from Gossypium barbadense into G.hirsutum,especially by molecular marker-assisted selection for specific chromosomes with high efficiency.
[Objective] Tolerance evaluation is the basis for cotton salinity-resistant breeding.[Method] Relative growth of cotton plants at seedling stage for 148 introgression lines was used to evaluate the tolerance to salinity.[Result] The results demonstrated that salt damage coefficients were significantly different from each other,with an average 61.22% ranging from1.34% to 100%.Based on the salt damage coefficient,148 introgression lines were divided into three groups;group 1(23.65%) was more tolerant than the tolerant parent with the salt damage coefficient less than 29.35%;group 2(39.19%)was more sensitive than the tolerant parent with the salt damage coefficient from 29.35% ―71.70%;group 3(37.16%) was more sensitive than the susceptible parent with the salt damage coefficient above 71.70%.[Condusion] Mapping of QTLs(Quantitative trait locus) for salt-tolerant traits indicated that 23 QTLs for tolerance-related traits were detected.Among them,12 QTLs were aggregately distributed on chromosomes A05,A12,D05 and D11.Our results demonstrate that it is feasible for screening salt-tolerant introgression lines from Gossypium barbadense into G.hirsutum,especially by molecular marker-assisted selection for specific chromosomes with high efficiency.
引文
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[19]栾明宝,郭香墨,张永山,等.利用陆地棉置换系进行海岛棉主要性状基因的染色体定位[J].棉花学报,2008,20(1):70-72.Luan Mingbao,Guo Xiangmo,Zhang Yongshan,et al.Gene location of main traits in specific chromosome or chromosome arms of G.barbadense by using chromosome substitution lines[J].Cotton Science,2008,20(1):70-72.
[20]王鹏,丁业掌,陆琼娴,等.陆地棉遗传标准系TM-1背景的海岛棉染色体片段置换系的培育[J].科学通报,2008,53(9):1065-1069.Wang Peng,Ding Yezhang,Lu Qiongxian,et al.Development of G.barbadense chromosome segment substitution lines in genetic standard line,TM-1 of G.hirsutum[J].Science Bulletin,2008,53(9):1065-1069.
[21]Wang P,Zhu Y,Song X,et al.Inheritance of long staple fiber quality traits of Gossypium barbadense in G.hirsutum background using CSILs[J].Theor Appl Genet,2012,124(8):1415.
[22]Paterson A H,Deverna J W,Lanini B,et al.Fine mapping of quantitative trait loci using selected overlapping recombinant chromosomes in an interspecies cross of tomato[J].Genetics,1990,124:735-742.
[23]Eshed Y,Zamir D.An genomic library of Lycopersicon pennellii in L.esculentum:a tool for fine mapping of genes[J].Euphytica,1994,79:175-179.
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[2]张国伟,路海玲,张雷,等.棉花萌发期和苗期耐盐性评价及耐盐指标筛选[J].应用生态学报,2011,22(8):2045-2053.Zhang Guowei,Lu Hailing,Zhang Lei,et al.Salt tolerance evaluation of cotton(Gossypium hirsutum)at its germinating and seedling stages and selection of related indices[J].Chin J Appl Ecol,2011,22(8):2045-2053.
[3]叶武威,王俊娟,樊宝相,等.棉花耐盐性鉴定及其研究进展[C].安阳:中国棉花学会2011年年会论文汇编.2011.Ye Wuwei,Wang Junjuan,Fan Baoxiang,et al.Evaluation and research on salinity-resistant cotton[C].Anyang:China Cotton Association 2011.2011.
[4]叶武威,刘金定.氯化钠和食用盐对棉花种子萌发的影响[J].中国棉花,1994,21(3):14-15.Ye Wuwei,Liu Jinding.Effects of sodium chloride and edible salt on cotton seed germination[J].China Cotton,1994,21(3):14-15.
[5]周桃华.Na Cl胁迫对棉子萌发及幼苗生长的影响[J].中国棉花,1995,22(4):11-12.Zhou Taohua.Effects of Na Cl Stress on seed germination and seedling growth of cotton[J].China Cotton,1995,22(4):11-12.
[6]Leidi E O,Nogales R,Lips S H.Effect of salinity on cotton plants grown under nitrate or ammonium nutrition at different calcium levels[J].Field Crops Research,1991,26(1):35-44.
[7]刘国强,刘金定,鲁黎明.棉花品种资源耐盐性鉴定研究[J].作物品种资源,1993(2):21-22.Liu Guoqiang,Liu Jinding,Lu Liming.Studies of the salt tolerance identification in cotton germplasm[J].China Seeds,1993(2):21-22.
[8]李函利,王省芬,王国宁,等.低酚棉种质资源耐盐性鉴定[J]植物遗传资源学报,2016,17(6):1099-1105.Li Hanli,Wang Xingfen,Wang Guoning,et al.Identification of salt tolerant germplasm resources in glandless cotton[J].Journal of Plant Genetic Resources,2016,17(6):1099-1105.
[9]叶武威,王俊娟,樊宝相,等.盐胁迫下不同耐盐性的陆地棉品种脱落规律及机理[J].棉花学报,2007,19(2):159-160,封三.Ye Wuwei,Wang Junjuan,Fan Baoxiang,et al.Abscission and its mechanism of three different salt-tolerant types of upland cotton under the stress of salinity[J].Cotton Science,2007,19(2):159-160,Cover 3.
[10]孙小芳,刘友良.棉花品种耐盐性鉴定指标可靠性的检验[J]作物学报,2001,27(6):794-802.Sun Xiaofang,Liu Youliang.Test on criteria of evaluating salt tolerance of cotton cultivars[J].Acta Agron Sin,2001,27:794-802.
[11]沈法富,尹承份,于元杰,等.棉花植株和花粉耐盐性的鉴定[J].作物学报,1997,23(5):620-625.Shen Fafu,Yin Chengfen,Yu Yuanjie,et al.Screening of whole plants and pollen grains of cotton for salt tolerance[J].Acta A-gronomica Sinica,1997,23(5):620-625.
[12]王立秋,张祖新,滕峰,等.染色体片段导入系在作物遗传育种研究中的应用[J].植物遗传资源学报,2012,13(1):98-104.Wang Liqiu,Zhang Zuxin,Teng Feng,et al.Application of chromosomal segment introgression lines in studying crop genetic breeding[J].Journal of Plant Genetic Resources,2012,13(1):98-104.
[13]张丽霞,刘丕庆,刘学义.染色体单片段代换系的构建及应用于QTL精细定位[J].分子植物育种,2004,2(3):743-746.Zhang Lixia,Liu Piqing,Liu Xueyi.Construction of chromosome single segment substitution lines and QTL fine mapping[J].Molecular Plant Breeding,2004,2(3):743-746.
[14]王玉民,席章营,尚爱兰,等.作物单片段代换系的构建及应用[J].中国农学通报,2008,24(3):67-71.Wang Yumin,Xi Zhangying,Shang Ailan,et al.Construction and application of single segment substitution lines in crops[J]Chinese Agricultural Science Bulletin,2008,24(3):67-71.
[15]梁燕.早熟陆地棉染色体片段代换系的构建及QTL初步定位[D].北京:中国农业科学院,2010:72-81.Liang Yan.Construction of chromosome segment substitution lines in early-mature upland cotton and QTL mapping[D].Beijing:Chinese Academy of Agricultural Sciences,2010:72-81.
[16]兰孟焦,杨泽茂,石玉真,等.陆海BC4F2和BC4F3代换系的评价及纤维产量与品质相关QTL的检测[J].中国农业科学,2011,44(15):3086-3097.Lan Mengjiao,Yang Zemao,Shi Yuzhen,et al.Evaluating substitution lines and detecting correlation QTL about fiber yield and quality in BC4F2and BC4F3generations from Gossypium hirsutum L.×Gossypium barbadense L.[J].Scientia Agricultura Sinica,2011,44(15):3086-3097.
[17]王立建.中国春小麦-粗山羊草染色体片段导入系的构建与分析[D].秦皇岛:河北科技师范学院,2011:20-52.Wang Lijian.Construction and analysis of chromosomal segment introgression line from Chinese spring wheat-aegilops tauschii[D].Qinhuangdao:Hebei Normal University of Science Technology,2011:20-52.
[18]李占伟.普通小麦异代换系的选育研究进展[J].陕西农业科学,2004(5):45-47.Li Zhanwei.Research progress of breeding common wheat alien substitution line[J].Shaanxi Agricultural Sciences,2004(5):45-47.
[19]栾明宝,郭香墨,张永山,等.利用陆地棉置换系进行海岛棉主要性状基因的染色体定位[J].棉花学报,2008,20(1):70-72.Luan Mingbao,Guo Xiangmo,Zhang Yongshan,et al.Gene location of main traits in specific chromosome or chromosome arms of G.barbadense by using chromosome substitution lines[J].Cotton Science,2008,20(1):70-72.
[20]王鹏,丁业掌,陆琼娴,等.陆地棉遗传标准系TM-1背景的海岛棉染色体片段置换系的培育[J].科学通报,2008,53(9):1065-1069.Wang Peng,Ding Yezhang,Lu Qiongxian,et al.Development of G.barbadense chromosome segment substitution lines in genetic standard line,TM-1 of G.hirsutum[J].Science Bulletin,2008,53(9):1065-1069.
[21]Wang P,Zhu Y,Song X,et al.Inheritance of long staple fiber quality traits of Gossypium barbadense in G.hirsutum background using CSILs[J].Theor Appl Genet,2012,124(8):1415.
[22]Paterson A H,Deverna J W,Lanini B,et al.Fine mapping of quantitative trait loci using selected overlapping recombinant chromosomes in an interspecies cross of tomato[J].Genetics,1990,124:735-742.
[23]Eshed Y,Zamir D.An genomic library of Lycopersicon pennellii in L.esculentum:a tool for fine mapping of genes[J].Euphytica,1994,79:175-179.
[24]Yamamoto T,Kuboki Y,Lin S Y,et al.Fine mapping of quantitative trait loci Hd-1,Hd-2 and Hd-3,controlling heading date of rice,as single Mendelian factors[J].Theor Appl Genet,199897:37-44.
[25]孙小芳,刘友良,陈沁.棉花耐盐性研究进展[J].棉花学报1998,10(3):118-124.Sun Xiaofang,Liu Youliang,Chen Qin.Recent progresses in studies on salinity tolerance in cotton[J].Acta Gossypii Sinica,1998,10(3):118-124.
[26]Levitt J.Responses of plants to environmental stress[M].2nd ed.New York:Academic Press,1980:13-30.
[27]吕有军,叶武威,祝水金.陆地棉种子萌发过程中盐处理对Na Cl胁迫的缓解作用[J].棉花学报,2005,17(4):256-257.LüYoujun,Ye Wuwei,Zhu Shuijin.Effects of additional salinity treatment on germination of upland cotton stressed by Na Cl[J].Cotton Science,2005,17(4):256-257.
[28]白旭,田长彦,胡明芳,等.盐分和温度以及光照对陆地棉种子萌发的影响[J].棉花学报,2006,18(4):238-241.Bai Xu,Tian Changyan,Hu Mingfang,et al.Influence of light temperature and salinity on the seed germination of Gossypium hirsutum L.[J].Cotton Science,2006,18(4):238-241.
[29]戴海芳,武辉,阿曼古丽·买买提阿力,等.不同基因型棉花苗期耐盐性分析及其鉴定指标筛选[J].中国农业科学,2014,47(7):1290-1300.Dai Haifang,Wu Hui,Amanguli Maimaitiali,et al.Analysis of salt-tolerance and determination of salt-tolerant evaluation indicators in cotton seedlings of different genotypes[J].Scientia A-gricultura Sinica,2014,47(7):1290-1300.
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