两种方法SCCA检测结果与组织鳞癌病理诊断的符合率比较
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摘要
目的比较电化学发光法(ECLIA)和流式荧光发光法(FFIA)检测鳞状细胞癌抗原(SCCA)用于相关组织鳞癌辅助诊断的价值,并比较两方法 SCCA结果与诊断符合率的一致性。方法采用ECLIA和FFIA法检测154例健康人群和236例确认鳞癌患者血清标本,其中肺鳞癌98例,宫颈鳞癌83例,头颈部鳞癌55例,比较两方法 SCCA结果诊断符合率的差异。结果 ECLIA法SCCA水平明显高于FFIA法(P<0.01),且两方法 SCCA结果相关性较好,相关系数r=0.843(P<0.01)。肺鳞癌、宫颈鳞癌患者SCCA结果诊断符合率(FFIA VS ECLIA)分别为40.82%VS 52.04%(P=0.043)和36.14%VS 57.14%(P=0.001),差别有统计学意义。健康体检人群、头颈部鳞癌患者SCCA结果诊断符合率(FFIA VS ECLIA)98.05%VS 98.70%(P=1)和16.36%VS 23.64%(P=0.388),差异无统计学意义。结论 ECLIA法SCCA的水平明显高于FFIA法,且二者结果相关较好,但ECLIA法在肺鳞癌、宫颈鳞癌中SCCA结果诊断符合率高于FFIA法。
Objective To compare the diagnostic value and diagnosis coincidence rate of electrochemiluminescence immunoassay(ECLIA)and flow fluorescence immunoluminometric assay(FFIA)in the detection of squamous cell carcinoma antigen(SCCA)for the diagnosis of squamous cell carcinoma.Methods ECLIA and FFIA methods were used to detect the serum SCCA level of 154 healthy people and 236 confirmed squamous cell carcinoma patients,including 98 cases of lung squamous cell carcinoma,83 cases of cervical squamous cell carcinoma and 55 cases of head and neck squamous cell carcinoma,for comparing the diagnostic coincidence rates of SCCA results of the two methods.Results The SCCA level of ECLIA was significantly higher than that of FFIA(P<0.01),with the correlation coefficient r=0.843(P<0.01).The two methods diagnostic coincidence rates of lung squamous cell carcinoma and cervical squamous cell carcinoma(FFIA vs.ECLIA)were 40.82% vs.52.04%(P=0.043)and 36.14% vs.57.14%(P=0.001),respectively.There was no statistically significant difference in the diagnostic coincidence rate(FFIA vs.ECLIA)of 98.05%vs.98.70%(P=1)and 16.36%vs.23.64%(P=0.388)of healthy people and head and neck squamous cell carcinoma patients.Conclusion The serum SCCA level of ECLIA was significantly higher than that of FFIA,with well correlation and higher diagnostic coincidence rate than that of FFIA of lung squamous cell carcinoma and cervical squamous cell carcinoma.
Objective To compare the diagnostic value and diagnosis coincidence rate of electrochemiluminescence immunoassay(ECLIA)and flow fluorescence immunoluminometric assay(FFIA)in the detection of squamous cell carcinoma antigen(SCCA)for the diagnosis of squamous cell carcinoma.Methods ECLIA and FFIA methods were used to detect the serum SCCA level of 154 healthy people and 236 confirmed squamous cell carcinoma patients,including 98 cases of lung squamous cell carcinoma,83 cases of cervical squamous cell carcinoma and 55 cases of head and neck squamous cell carcinoma,for comparing the diagnostic coincidence rates of SCCA results of the two methods.Results The SCCA level of ECLIA was significantly higher than that of FFIA(P<0.01),with the correlation coefficient r=0.843(P<0.01).The two methods diagnostic coincidence rates of lung squamous cell carcinoma and cervical squamous cell carcinoma(FFIA vs.ECLIA)were 40.82% vs.52.04%(P=0.043)and 36.14% vs.57.14%(P=0.001),respectively.There was no statistically significant difference in the diagnostic coincidence rate(FFIA vs.ECLIA)of 98.05%vs.98.70%(P=1)and 16.36%vs.23.64%(P=0.388)of healthy people and head and neck squamous cell carcinoma patients.Conclusion The serum SCCA level of ECLIA was significantly higher than that of FFIA,with well correlation and higher diagnostic coincidence rate than that of FFIA of lung squamous cell carcinoma and cervical squamous cell carcinoma.
引文
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[2]Izuhara K,Yamaguchi Y,Ohta S,et al.Squamous Cell Carcinoma Antigen 2(SCCA2,SERPINB4):An Emerging Biomarker for Skin Inflammatory Diseases[J].Int J Mol Sci,2018,19(4).pii:E1102.
[3]Ohta S,Shibata R,Nakao Y,et al.The usefulness of combined measurements of squamous cell carcinoma antigens 1and 2in diagnosing atopic dermatitis[J].Ann Clin Biochem,2012,49(Pt3):277.
[4]闫丽隽,赵欣,邵淑丽,等.子宫颈鳞状细胞癌中SCCA1和SC-CA2的分子表达及其意义[J].肿瘤研究与临床,2011,23(3):157.
[5]刘盼,多力昆·吾甫尔,蔡志刚,等.维吾尔族口腔鳞状细胞癌患者中血清鳞状细胞癌抗原的表达研究[J].国际口腔医学杂志,2016,43(6):645.
[6]Suzuki M,Deng Z,Hasegawa M,et al.Squamous cell carcinoma antigen production in nasal inverted papilloma[J].Am J Rhinol Allergy,2012,26(5):365.
[7]孙哲.河南食管癌高发区人群SCCA1、Prx1表达与食管癌预后相关因素分析[D].郑州大学,2006.
[8]Biasiolo A,Tono N,Ruvoletto M,et al.IgM-linked SerpinB3and SerpinB4in sera of patients with chronic liver disease[J].PLoSOne,2012,7(7):e40658.
[9]Li S,Gao Y,Yang B,et al.Squamous cell carcinoma antigen 1and2mRNA and a new variant expressed in hepatocellular carcinoma[J].Neoplasma,2014,61(6):718.
[10]罗士来,庄小青,夏前凤,等.基于Excel VBA的EP9-A2文件数据处理模板的建立与应用[J].临床检验杂志,2013,31(8):634.
[11]Guo Z,Wu L,Hu Y,et al.Potential-resolved“in-electrode”type electrochemiluminescence immunoassay based on functionalized g-C(3)N(4)nanosheet and Ru-NH(2)for simultaneous determination of dual targets[J].Biosens Bioelectron,2017,95:27.