毛细管电泳电化学发光法快速分离检测水果中的精胺
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摘要
目的:建立水果中精胺含量测定的毛细管电泳电化学发光(CE-ECL)法。方法:利用CE-ECL技术建立了一种高效快速准确的测定桑椹、苹果、草莓中精胺含量的的方法。结果:精胺标准溶液在2×10~(-4)~3×10~(-1)mmol/L浓度范围内与ECL强度呈良好的线性关系,其线性方程为I=1114.2c+99.41,相关系数r=0.9996(n=5),检出限(S/N=3)为9.9×10~(-5)mmol/L,加标回收率分别为95.14%~101.15%、98.97%~100.82%和100.57%~106.11%。结论:该方法简单、准确,重复性好,可用于研究果蔬采后保鲜贮藏和延长货架期。
Objective: To establish a capillary electrophoresis with electrochemiluminescence( CE-ECL) method for determination of spermidine in fruit. Methods: An efficient,rapid and accurate analytical approach was developed for the determination of spermine in mulberry,apple,strawberry by CE-ECL technique.Results: The ECL intensity was linear with the concentrations of spermine over the ranges from 2 × 10~(-4)~ 3 × 10~(-1) mmol/L. The linear equation was I = 1114.2 c + 99.41,correlation coefficient r = 0.9996( n = 5) with detection limit( S/N = 3) of 9.9 × 10~(-5) mmol/L. The adding standard recoveries were 95.14% ~ 101.15%,98.97% ~ 100.82% and 100.57% ~ 106.11%,respectively. Conclusions: This method is simple,accurate,reproducible and can be used for the study of fruit fresh-keeping,and prolonging processing period.
Objective: To establish a capillary electrophoresis with electrochemiluminescence( CE-ECL) method for determination of spermidine in fruit. Methods: An efficient,rapid and accurate analytical approach was developed for the determination of spermine in mulberry,apple,strawberry by CE-ECL technique.Results: The ECL intensity was linear with the concentrations of spermine over the ranges from 2 × 10~(-4)~ 3 × 10~(-1) mmol/L. The linear equation was I = 1114.2 c + 99.41,correlation coefficient r = 0.9996( n = 5) with detection limit( S/N = 3) of 9.9 × 10~(-5) mmol/L. The adding standard recoveries were 95.14% ~ 101.15%,98.97% ~ 100.82% and 100.57% ~ 106.11%,respectively. Conclusions: This method is simple,accurate,reproducible and can be used for the study of fruit fresh-keeping,and prolonging processing period.
引文
[1]Chen Zhao,Zhu Chuanhe,Han Ziqiang.Effects of aqueous chlorine dioxide treatment on nutritional components and shelflife of mulberry fruit(Morus alba L.)[J].Journal of Bioscience and Bioengineering,2011,111(6):675-681.
[2]聂青玉,刘丹,王燕飞,等.壳寡糖处理对草莓贮藏品质的影响[J].农产品加工(创新版),2010(7):31-33.
[3]朱新卫,张辉,潘俨,等.精胺处理对新疆赛买提杏冷藏品质的影响[J].保鲜与加工,2014,14(5):20-23,30.
[4]张政,王倩,吴斌,等.外源精胺对哈密大枣冷藏期间贮藏特性的影响[J].食品研究与开发,2016,14(37):163-167.
[5]刘春泉,宋江峰,王远,等.外源精胺对菜用大豆贮藏冷害及蔗糖代谢的影响[J].中国农业科学,2015,48(8):1588-1596.
[6]MR Rui,IM Valente,JA Rodrigues.Analysis of biogenic amines in wines by salting-out assisted liquid-liquid extraction and high-performance liquid chromatography with fluorimetric detection[J].Talanta,2014,124(124):146.
[7]郑晓菊,许泳吉,刘云云,等.高效液相色谱法测定贝壳类海洋生物中的多胺含量[J].青岛科技大学学报自然科学版,2014,35(5):446-450.
[8]林绍艳,张芳,徐颖洁.植物中多胺含量超高效液相色谱法的建立[J].南京农业大学学报,2016,39(3):358-365.
[9]曾立威,蔡翔宇,吴玉杰,等.超高效液相色谱法同时快速测定多种动物源食品中9种生物胺的含量[J].食品安全质量检测学报,2017,8(3):968-074.
[10]BD Rio,B Redruello,DM Linares,et al.The dietary biogenic amines tyramine and histamine show synergistic toxicity towards intestinal cells in culture[J].Food Chemistry,2017,218:249-255.
[11]食品中多胺的测定[S].GB 5009.208-2016.
[12]姜随意,吴业宾,王浩,等.用于组胺检测的新型电化学传感技术研究[J].食品研究与开发,2017,4(38):113-118.
[13]张冰宇,蔡晓蓉,尹艳艳,等.毛细管电色谱-激光诱导荧光检测法分析食品中的生物胺[J].色谱,2017,35(3):345-350.
[14]鲜苹果[S].GB/T 10651-2008.
[15]草莓等级规格[S].NY/T 17889-2009.
[16]桑椹(桑果)[S].GB/T 29572-2013.
[17]Hu Lianzhe,Xu Guobao.Applications and trends in electrochemiluminescence[J].Chemical Society Reviews,2010,39:3275-3304.
[18]寿林均,程巧鸳,黄柳倩,等.高效液相色谱法测定益之康胶囊中总蒽酮(大黄素和大黄素甲醚)的含量[J].食品工业科技,2017(7):252-256,267.
[2]聂青玉,刘丹,王燕飞,等.壳寡糖处理对草莓贮藏品质的影响[J].农产品加工(创新版),2010(7):31-33.
[3]朱新卫,张辉,潘俨,等.精胺处理对新疆赛买提杏冷藏品质的影响[J].保鲜与加工,2014,14(5):20-23,30.
[4]张政,王倩,吴斌,等.外源精胺对哈密大枣冷藏期间贮藏特性的影响[J].食品研究与开发,2016,14(37):163-167.
[5]刘春泉,宋江峰,王远,等.外源精胺对菜用大豆贮藏冷害及蔗糖代谢的影响[J].中国农业科学,2015,48(8):1588-1596.
[6]MR Rui,IM Valente,JA Rodrigues.Analysis of biogenic amines in wines by salting-out assisted liquid-liquid extraction and high-performance liquid chromatography with fluorimetric detection[J].Talanta,2014,124(124):146.
[7]郑晓菊,许泳吉,刘云云,等.高效液相色谱法测定贝壳类海洋生物中的多胺含量[J].青岛科技大学学报自然科学版,2014,35(5):446-450.
[8]林绍艳,张芳,徐颖洁.植物中多胺含量超高效液相色谱法的建立[J].南京农业大学学报,2016,39(3):358-365.
[9]曾立威,蔡翔宇,吴玉杰,等.超高效液相色谱法同时快速测定多种动物源食品中9种生物胺的含量[J].食品安全质量检测学报,2017,8(3):968-074.
[10]BD Rio,B Redruello,DM Linares,et al.The dietary biogenic amines tyramine and histamine show synergistic toxicity towards intestinal cells in culture[J].Food Chemistry,2017,218:249-255.
[11]食品中多胺的测定[S].GB 5009.208-2016.
[12]姜随意,吴业宾,王浩,等.用于组胺检测的新型电化学传感技术研究[J].食品研究与开发,2017,4(38):113-118.
[13]张冰宇,蔡晓蓉,尹艳艳,等.毛细管电色谱-激光诱导荧光检测法分析食品中的生物胺[J].色谱,2017,35(3):345-350.
[14]鲜苹果[S].GB/T 10651-2008.
[15]草莓等级规格[S].NY/T 17889-2009.
[16]桑椹(桑果)[S].GB/T 29572-2013.
[17]Hu Lianzhe,Xu Guobao.Applications and trends in electrochemiluminescence[J].Chemical Society Reviews,2010,39:3275-3304.
[18]寿林均,程巧鸳,黄柳倩,等.高效液相色谱法测定益之康胶囊中总蒽酮(大黄素和大黄素甲醚)的含量[J].食品工业科技,2017(7):252-256,267.