小儿扶脾颗粒的质量标准研究
|
摘要
目的建立小儿扶脾颗粒的质量标准。方法采用薄层色谱法对小儿扶脾颗粒中白术、陈皮、莲子进行定性鉴别;采用高效液相色谱法测定了陈皮中橙皮苷的含量:色谱柱为Agilent C_(18)(TC)(250mm×4. 6mm,5μm);流动相为甲醇-水(32∶68);流速0. 8mL·min~(-1);检测波长为283nm。结果薄层色谱斑点清晰,阴性无干扰;高效液相色谱法测定小儿扶脾颗粒中橙皮苷含量,测得橙皮苷对照品进样量在0. 0412~1. 03μg范围内线性关系良好(r=0. 9998),平均加样回收率为101. 84%,RSD为2. 98%。结论该方法简便、准确、重复性好,可以有效控制小儿扶脾颗粒的质量。
OBJECTIVE To establish the quality standard for Xiao' er Fupi Granules. METHODS TLC was used for the qualitative identification of atractylodes macrocephala rhizoma,tangerine peel and lotus seeds. HPLC was used to determine the content of hesperidin. The separation was performed on an Agilent C_(18)( TC)( 250 mm ×4. 6 mm,5μm) coulme with mobile phase consisting of methanol-water( 32∶ 68). The detection wavelength was 283 nm and the flow rate was 0. 8 mL·min~(-1). RESULTS TLC spots were clear and well-separated without negative interference. The linear range of hesperidin was 0. 0412 ~ 1. 03μg( r = 0. 9998) with an average recovery of 101. 84%( RSD = 2. 98%). CONCLUSION The method is simple,accurate and reproducibility. It can be used for the quality control of Xiao'er Fupi Granules.
OBJECTIVE To establish the quality standard for Xiao' er Fupi Granules. METHODS TLC was used for the qualitative identification of atractylodes macrocephala rhizoma,tangerine peel and lotus seeds. HPLC was used to determine the content of hesperidin. The separation was performed on an Agilent C_(18)( TC)( 250 mm ×4. 6 mm,5μm) coulme with mobile phase consisting of methanol-water( 32∶ 68). The detection wavelength was 283 nm and the flow rate was 0. 8 mL·min~(-1). RESULTS TLC spots were clear and well-separated without negative interference. The linear range of hesperidin was 0. 0412 ~ 1. 03μg( r = 0. 9998) with an average recovery of 101. 84%( RSD = 2. 98%). CONCLUSION The method is simple,accurate and reproducibility. It can be used for the quality control of Xiao'er Fupi Granules.
引文
[1]校合香,邹阳,邓芬,等.健脾肥儿口服液质量标准研究[J].中国药业,2005,14(5):32-33.
[2]张丽梅,李俊,褚阳,等.清源调脂胶囊质量标准的提高[J].中国药师,2016,19(10):1972-1975.
[3]程艳芹,纪松岗,赵丽艳,等.胃灵颗粒质量标准的提高[J].中国药师,2015,18(10):1720-1723.
[4]徐小芳,陈文婷,刘敏,等.复方附子口服液质量标准的研究[J].中成药,2016,38(4):815-819.
[5]刘金坤,王琴,白殊同,等.六味地黄口服液薄层色谱鉴别研究[J].中国药业,2016,25(13):68-70.
[6]李昂,阚红玉,张囡. HPLC法同时测定疏表灵颗粒中绿原酸、橙皮苷、黄芩苷的含量[J].中国药房,2016,27(27):3872-3874.
[7]江萌,陈红梅,郑辉. HPLC法同时测定养胃舒颗粒中绿原酸、牡荆素鼠李糖苷及橙皮苷的含量[J].中国药师,2014,17(3):412-414.
[8]孙艳涛. HPLC双波长法同时测定消石利胆胶囊中芍药苷、橙皮苷、黄芩苷和大黄酚的含量[J].中国药师,2016,19(4):801-803.
[9]任卫琼,万艳群,李珊,等.乳核分散片质量标准的建立[J].中国药师,2014,17(3):394-397.
[10]范蕾,刘敏,吴查青,等. HPLC法同时测定四季感冒胶囊中橙皮苷和连翘苷的含量[J].中国药师,2015,18(6):1054-1056.
[11]夏文,熊灿琼.通窍耳聋丸的质量标准[J].中国药师,2010,13(11):1670-1671.
[12]国家药典委员会编.中国药典:一部[S]. 2015年版.北京:中国医药科技出版社,2015,191.
[2]张丽梅,李俊,褚阳,等.清源调脂胶囊质量标准的提高[J].中国药师,2016,19(10):1972-1975.
[3]程艳芹,纪松岗,赵丽艳,等.胃灵颗粒质量标准的提高[J].中国药师,2015,18(10):1720-1723.
[4]徐小芳,陈文婷,刘敏,等.复方附子口服液质量标准的研究[J].中成药,2016,38(4):815-819.
[5]刘金坤,王琴,白殊同,等.六味地黄口服液薄层色谱鉴别研究[J].中国药业,2016,25(13):68-70.
[6]李昂,阚红玉,张囡. HPLC法同时测定疏表灵颗粒中绿原酸、橙皮苷、黄芩苷的含量[J].中国药房,2016,27(27):3872-3874.
[7]江萌,陈红梅,郑辉. HPLC法同时测定养胃舒颗粒中绿原酸、牡荆素鼠李糖苷及橙皮苷的含量[J].中国药师,2014,17(3):412-414.
[8]孙艳涛. HPLC双波长法同时测定消石利胆胶囊中芍药苷、橙皮苷、黄芩苷和大黄酚的含量[J].中国药师,2016,19(4):801-803.
[9]任卫琼,万艳群,李珊,等.乳核分散片质量标准的建立[J].中国药师,2014,17(3):394-397.
[10]范蕾,刘敏,吴查青,等. HPLC法同时测定四季感冒胶囊中橙皮苷和连翘苷的含量[J].中国药师,2015,18(6):1054-1056.
[11]夏文,熊灿琼.通窍耳聋丸的质量标准[J].中国药师,2010,13(11):1670-1671.
[12]国家药典委员会编.中国药典:一部[S]. 2015年版.北京:中国医药科技出版社,2015,191.