小动物PET/CT显像考察麻黄碱干预棕色脂肪~(18)F-FDG摄取的研究
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摘要
目的:采用小动物PET/CT研究不同剂量麻黄碱(Ephedrine,Eph)与温度对棕色脂肪(Brown Adipose Tissue,BAT)摄取18F-氟代脱氧葡萄糖(18F-fluoro-deoxyglucose,18F-FDG)的影响。方法:将昆明小鼠腹腔注射18F-FDG或18F-FDG+Eph(40 mg/kg、80 mg/kg和160 mg/kg),放置于不同实验温度条件下,1 h后采用小动物PET/CT对小鼠进行全身PET/CT扫描,通过Inveon Research Workplace(IRW)软件对小鼠肩胛处BAT摄取18F-FDG的标准化摄取值(Standardised uptake value,SUV)进行分析。结果 :25℃和15℃未使用Eph干预的BAT SUV值分别为1.67±0.49和2.7±0.26;25℃和15℃使用Eph(40 mg/kg)干预BAT SUV值分别为2.20±0.36和5.97±0.21。温度为25℃,Eph分别为40 mg/kg、80 mg/kg和160 mg/kg时BAT SUV值分别为2.20±0.36、3.33±0.31和6.73±0.50。结论 :低温可以刺激BAT的能量代谢增高,同时随着Eph的剂量增高BAT的能量代谢程度也随之增加。小动物PET/CT能有效检测小鼠体内肩胛处BAT对18F-FDG的代谢情况。
Objective: To investigato the effect of different doses of Ephedrine(Eph) and temperature on18 FFDG uptake in brown adipose tissue(BAT) of Kungming mice using small-animal PET/CT. Methods:18F-FDG or a mixture of18F-FDG and Eph(40 mg/kg, 80 mg/kg or 160 mg/kg of body weight) were injected into the peritoneal cavity of mice at different temperatures. One hour after injection, the BAT activity was assessed by measuring SUV with Micro PET/CT. Results: In mice without ingection of Eph, the SUV of18F-FDG, was 1.67 ±0.49 at 25 ℃, and 2.7 ± 0.26 at 15 ℃, respectively. In mice injected with 40 mg/kg of Eph, the SUV was 2.20 ±0.36 at 25 ℃, and 5.97 ± 0.21 at 15 ℃, respectively. At 25 ℃, mice injected with 40 mg/kg, 80 mg/kg and 160mg/kg of Eph, the The SUV of18F-FDG was 2.20 ± 0.36, 3.33 ± 0.31 and 6.73 ± 0.50 respectively. Conclusion:The cold exposure could cause a greater increase in18F-FDG uptake in BAT, and the metabolism level of BAT was will increase with the increased dose off. Small-animal PET/C is suitable for the study of18F-FDG metabolism in interscapular BAT of mice.
Objective: To investigato the effect of different doses of Ephedrine(Eph) and temperature on18 FFDG uptake in brown adipose tissue(BAT) of Kungming mice using small-animal PET/CT. Methods:18F-FDG or a mixture of18F-FDG and Eph(40 mg/kg, 80 mg/kg or 160 mg/kg of body weight) were injected into the peritoneal cavity of mice at different temperatures. One hour after injection, the BAT activity was assessed by measuring SUV with Micro PET/CT. Results: In mice without ingection of Eph, the SUV of18F-FDG, was 1.67 ±0.49 at 25 ℃, and 2.7 ± 0.26 at 15 ℃, respectively. In mice injected with 40 mg/kg of Eph, the SUV was 2.20 ±0.36 at 25 ℃, and 5.97 ± 0.21 at 15 ℃, respectively. At 25 ℃, mice injected with 40 mg/kg, 80 mg/kg and 160mg/kg of Eph, the The SUV of18F-FDG was 2.20 ± 0.36, 3.33 ± 0.31 and 6.73 ± 0.50 respectively. Conclusion:The cold exposure could cause a greater increase in18F-FDG uptake in BAT, and the metabolism level of BAT was will increase with the increased dose off. Small-animal PET/C is suitable for the study of18F-FDG metabolism in interscapular BAT of mice.
引文
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14 .Vosselman MJ,Van Marken Lichtenbelt WD,Schrauwen P.Energy dissipation in brown adipose tissue:From mice to men[J].Molecular and cellular endocrinology,2013,379(1):43-50.
2 .Saito M,Okamatsu-Ogura Y,Matsushita M,et al.High incidence of metabolically active brown adipose tissue in healthy adult humans effects of cold exposure and adiposity[J].Diabetes,2009,58(7):1526-1531.
3 .Vijgen G,Bouvy ND,Teule GJ J,et al.Increase in brown adipose tissue activity after weight loss in morbidly obese subjects[J].The Journal of Clinical Endocrinology&Metabolism,2012,97(7):E1229-E1233.
4 .Van Marken Lichtenbelt WD,Vanhommerig J W,Smulders N M,et al.Cold-activated brown adipose tissue in healthy men[J].New England Journal of Medicine,2009,360(15):1500-1508.
5 .Cannon B,Nedergaard JA N.Brown adipose tissue:function and physiological significance[J].Physiological reviews,2004,84(1):277-359.
6 .Baba S,Tatsumi M,Ishimori T,et al.Effect of nicotine and ephedrine on the accumulation of18F-FDG in brown adipose tissue[J].Journal of Nuclear Medicine,2007,48(6):981-986.
7 .Borga M,Virtanen KA,Romu T,et al.Brown adipose tissue in humans:detection and functional analysis using PET(positron emission tomography),MRI(magnetic resonance imaging),and DECT(dual energy computed tomography)[J].Methods Enzymol,2014,537:141-159.
8 .Fueger B J,Czernin J,Hildebrandt I,et al.Impact of animal handling on the results of18F-FDG PET studies in mice[J].Journal of Nuclear Medicine,2006,47(6):999-1006.
9 .Limberger RP,Jacques ALB,Schmitt GC,et al.Pharmacological Effects of Ephedrine[M].Natural Products.Springer Berlin Heidelberg,2013:1217-1237.
10 .Carey AL,Pajtak R,Formosa MF,et al.Chronic ephedrine administration decreases brown adipose tissue activity in a randomised controlled human trial:implications for obesity[J].Diabetologia,2015,1-10.
11 .Senthamizhchelvan S,Tahari A,Gajwani P,et al.Orally administered18F-FDG:Human biodistribution and dosimetry[J].Journal of Nuclear Medicine,2014,55(supplement1):1133-1133.
12 .Yao R,Lecomte R,Crawford ES.Small-animal PET:what is it,and why do we need it?[J].Journal of nuclear medicine technology,2012,40(3):157-165.
13 .Ouellet V,LabbéSM,Blondin DP,et al.Brown adipose tissue oxidative metabolism contributes to energy expenditure during acute cold exposure in humans[J].The Journal of clinical investigation,2012,122(2):545.
14 .Vosselman MJ,Van Marken Lichtenbelt WD,Schrauwen P.Energy dissipation in brown adipose tissue:From mice to men[J].Molecular and cellular endocrinology,2013,379(1):43-50.