纳洛酮对大鼠大脑皮质c-fos mRNA表达的影响
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摘要
为研究纳洛酮对小型猪特异性麻醉剂(XFM)麻醉下大鼠大脑皮质c-fos mRNA表达的影响,探讨纳洛酮催醒XFM麻醉大鼠与大脑皮质c-fos基因的关系。将78只SD纯种大鼠随机分为空白对照组、XFM对照组、XFM+纳洛酮组、XFM+生理盐水组,采用实时定量PCR技术检测大脑皮质内c-fos mRNA表达量。结果表明,腹腔注射XFM后引起大鼠大脑皮质c-fos mRNA高效表达,各时期c-fos mRNA表达与空白对照组比较差异极显著(P<0.01);纳洛酮注射后引起XFM麻醉大鼠大脑皮质c-fos mRNA表达量减少,与XFM对照组比较差异显著(P<0.01或P<0.05)。结果提示,大脑皮质c-fos基因参与了纳洛酮颉颃XFM的麻醉作用,纳洛酮抑制XFM诱导大鼠大脑皮质c-fos基因表达,可能是纳洛酮催醒XFM麻醉大鼠的重要机理之一。
To investigate relationship between naloxon woke up anaesthetic rat by XFM and c-fos gene,by study on effect of naloxon on the expression of c-fos mRNA in the rat cerebral cortex,78 SD rats were divided randomly into blank control group,XFM group,XFM add naloxon group,XFM add saline group.The contents of c-fos mRNA was measured by RT-PCR in cerebral cortex.The contents of c-fos mRNA was obviously increased in the XFM anesthesia group as compared with blank control group(P<0.01).The contents of c-fos mRNA was significantly decreased after naloxon administrated as compared with XFM group(P<0.05 or P<0.01).The results indicated that naloxon palinesthesia action was concerned with c-fos gene in cerebral cortex.Naloxon attenuates expression of c-fos gene in the rat cerebral cortex may be an important mechanism that naloxon woke up anaesthetic rat by XFM.
To investigate relationship between naloxon woke up anaesthetic rat by XFM and c-fos gene,by study on effect of naloxon on the expression of c-fos mRNA in the rat cerebral cortex,78 SD rats were divided randomly into blank control group,XFM group,XFM add naloxon group,XFM add saline group.The contents of c-fos mRNA was measured by RT-PCR in cerebral cortex.The contents of c-fos mRNA was obviously increased in the XFM anesthesia group as compared with blank control group(P<0.01).The contents of c-fos mRNA was significantly decreased after naloxon administrated as compared with XFM group(P<0.05 or P<0.01).The results indicated that naloxon palinesthesia action was concerned with c-fos gene in cerebral cortex.Naloxon attenuates expression of c-fos gene in the rat cerebral cortex may be an important mechanism that naloxon woke up anaesthetic rat by XFM.
引文
[1]Kondo T,Ohshima T,Sato Y,et al.Immunohisto-chemical study on the expression of c-Fos and c-Jun inhuman skin wounds[J].Histochem J,2000,32(8):509-514.
[2]Yoshida k,Maruyama M,Nagashima K,et al.Fos ex-pression induced by warming the preoptic area in rats[J].Brain Res,2002,933(2):109-117.
[3]许霁虹,王多友,张铁铮,等.异丙酚对应激大鼠脑组织不同区域c-fos mRNA表达的影响[J].中华麻醉学杂志,2001,21(11):679-681.
[4]Nagata A,Nakao S,Nishizava N,et al.Xenon inhibitsenhances ketamine-induced c-Fos expression in the ratposterior cingulate and retrosplenial cortices[J].Anesh Analg,2001,92(2):362-368.
[5]卢德章,范宏刚,于世明,等.替来他明诱导大鼠中枢神经系统c-fos基因的表达[J].畜牧兽医学报,2010,41(7):878-882.
[6]Moresco A,Larsen R S,Sleeman J M,et al.Use ofnaloxone to reverse carfentanil citrateinduced hypox-emia and cardiopulmonary depression in Rocky Moun-tain wapiti(Cervus elaphus nelsoni)[J].J Zoo WildMed,2001,32:81-89.
[7]Masahiko T,Kimitoshi S,Masaki H,et al.Naloxonereversal of opioid anesthesia revisited:clinical evalua-tion and plasma concentration analysis of continuousnaloxone infusion after anesthesia with high-dose fen-tanyl[J].J Anesth,2004,18:1-8.
[8]赵丽云,叶铁虎,张银中,等.小剂量纳洛酮在术后吗啡静脉自控镇痛中的应用[J].中国医学科学院学报,2005,27(2):228-231.
[9]Scott L V,Rathore O,Dinan T G,et al.Pituitary-ad-renal response to naloxone in nonulcer dyspepsia:pre-liminary evidence for a reduction in central opioid tone[J].Digestion,2002,65:67-72.
[10]Sinz E H,Kofke W A,Garman R H.Phenytoin,mid-azolam,and naloxone protect against fentanyl-inducedbrain damage in rats[J].Anesth Analg,2000,91:1443-1449.
[11]Kakinohana M,Marsala M,Carter C,et al.Neuraxialmorphine may trigger transient motor dysfunction af-ter a noninjurious interval of spinal cord ischemia:aclinical and experimental study[J].Anesthesiology,2003,98:862-870.
[12]Liu M,Wittbrodt E.Low-dose oral naloxone reversesopioid-induced constipation and analgesia[J].J PainSymptom Manage,2002,23:48-53.
[13]卢德章.小型猪特异性麻醉颉颃剂的研制及其催醒机理的研究[D].哈尔滨:东北农业大学,2011:119-120.
[14]马昆,张娈松,卢德章,等.小型猪复合麻醉剂特异性颉颃剂对小型猪各项血液指标的影响[J].中国兽医杂志,2011,47(1):36-38.
[15]谭丽娟,马昆,范宏刚,等.小型猪特异性麻醉复合颉颃剂对小型猪脑电影响的试验[J].中国兽医杂志,2011,47(4):23-25.
[16]许霁虹,张铁铮,刘晓江,等.氯胺酮麻醉引起c-fos基因在大鼠大脑皮质的表达[J].临床麻醉学杂志,2005,21(2):116-117.
[17]卢德章,范宏刚,于世明.隆朋对替来他明/唑拉西泮诱导的大鼠不同脑区c-fos基因表达的影响[J].中国兽医杂志,2011,47(6):23-25.
[2]Yoshida k,Maruyama M,Nagashima K,et al.Fos ex-pression induced by warming the preoptic area in rats[J].Brain Res,2002,933(2):109-117.
[3]许霁虹,王多友,张铁铮,等.异丙酚对应激大鼠脑组织不同区域c-fos mRNA表达的影响[J].中华麻醉学杂志,2001,21(11):679-681.
[4]Nagata A,Nakao S,Nishizava N,et al.Xenon inhibitsenhances ketamine-induced c-Fos expression in the ratposterior cingulate and retrosplenial cortices[J].Anesh Analg,2001,92(2):362-368.
[5]卢德章,范宏刚,于世明,等.替来他明诱导大鼠中枢神经系统c-fos基因的表达[J].畜牧兽医学报,2010,41(7):878-882.
[6]Moresco A,Larsen R S,Sleeman J M,et al.Use ofnaloxone to reverse carfentanil citrateinduced hypox-emia and cardiopulmonary depression in Rocky Moun-tain wapiti(Cervus elaphus nelsoni)[J].J Zoo WildMed,2001,32:81-89.
[7]Masahiko T,Kimitoshi S,Masaki H,et al.Naloxonereversal of opioid anesthesia revisited:clinical evalua-tion and plasma concentration analysis of continuousnaloxone infusion after anesthesia with high-dose fen-tanyl[J].J Anesth,2004,18:1-8.
[8]赵丽云,叶铁虎,张银中,等.小剂量纳洛酮在术后吗啡静脉自控镇痛中的应用[J].中国医学科学院学报,2005,27(2):228-231.
[9]Scott L V,Rathore O,Dinan T G,et al.Pituitary-ad-renal response to naloxone in nonulcer dyspepsia:pre-liminary evidence for a reduction in central opioid tone[J].Digestion,2002,65:67-72.
[10]Sinz E H,Kofke W A,Garman R H.Phenytoin,mid-azolam,and naloxone protect against fentanyl-inducedbrain damage in rats[J].Anesth Analg,2000,91:1443-1449.
[11]Kakinohana M,Marsala M,Carter C,et al.Neuraxialmorphine may trigger transient motor dysfunction af-ter a noninjurious interval of spinal cord ischemia:aclinical and experimental study[J].Anesthesiology,2003,98:862-870.
[12]Liu M,Wittbrodt E.Low-dose oral naloxone reversesopioid-induced constipation and analgesia[J].J PainSymptom Manage,2002,23:48-53.
[13]卢德章.小型猪特异性麻醉颉颃剂的研制及其催醒机理的研究[D].哈尔滨:东北农业大学,2011:119-120.
[14]马昆,张娈松,卢德章,等.小型猪复合麻醉剂特异性颉颃剂对小型猪各项血液指标的影响[J].中国兽医杂志,2011,47(1):36-38.
[15]谭丽娟,马昆,范宏刚,等.小型猪特异性麻醉复合颉颃剂对小型猪脑电影响的试验[J].中国兽医杂志,2011,47(4):23-25.
[16]许霁虹,张铁铮,刘晓江,等.氯胺酮麻醉引起c-fos基因在大鼠大脑皮质的表达[J].临床麻醉学杂志,2005,21(2):116-117.
[17]卢德章,范宏刚,于世明.隆朋对替来他明/唑拉西泮诱导的大鼠不同脑区c-fos基因表达的影响[J].中国兽医杂志,2011,47(6):23-25.