华山新麦草易位系抗黄矮病的鉴定
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摘要
小麦黄矮病在田间由麦蚜传播大麦黄矮病毒(BYDV)感染引起。为给优质抗BYDV小麦新品种提供重要种质资源,采用堆测法种植材料,利用饲毒蚜虫接种病毒BYDV-GAV的方式,对27份华山新麦草易位系材料进行了抗病性鉴定,比较了接种病毒后各材料的病情指数及其千粒重。结果表明,27份华山新麦草易位系中,5份材料的病情指数介于13.56～18.89,表现为抗BYDV-GAV;9份材料的病情指数是介于27.78～48.89,表现为感BYDV-GAV;其余13份材料的病情指数均大于50.0,表现高感BYDV-GAV。不同华山新麦草易位系对BYDV-GAV抗性差异较大,筛选出的5份抗黄矮病材料可为培育优质抗病毒新品种提供重要种质资源。
Yellow dwarfis is caused by wheat aphid transmission of viruses(BYDV). With hill plot technique to sow wheat later,resistance identifications of 27 P.huashanica translocation lines is to BYDV-GAV were conducted by inoculation of viruliferous aphids in the field. The results showed that the disease index of 5 P.huashanica translocation lines existed in the range of 13.56-18.89,indicating them better resistance to infection of BYDV-GAV. Additionally,9 lines of them with disease index between 27.78 and 48.89,were susceptible to infection of BYDV-GAV. Other 13 lines with disease index over 50.0,lines highly susceptible to yellow dwarf virus. Identification of disease resistance revealed that the translocation lines of P.huashanica contained disease-resistant,disease-susceptible and highly susceptible types,and 5 lines from which had been screened may provide important germplasm resources for high-quality antiviral variety breeding.
Yellow dwarfis is caused by wheat aphid transmission of viruses(BYDV). With hill plot technique to sow wheat later,resistance identifications of 27 P.huashanica translocation lines is to BYDV-GAV were conducted by inoculation of viruliferous aphids in the field. The results showed that the disease index of 5 P.huashanica translocation lines existed in the range of 13.56-18.89,indicating them better resistance to infection of BYDV-GAV. Additionally,9 lines of them with disease index between 27.78 and 48.89,were susceptible to infection of BYDV-GAV. Other 13 lines with disease index over 50.0,lines highly susceptible to yellow dwarf virus. Identification of disease resistance revealed that the translocation lines of P.huashanica contained disease-resistant,disease-susceptible and highly susceptible types,and 5 lines from which had been screened may provide important germplasm resources for high-quality antiviral variety breeding.
引文
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[2]TAO Y,MAN J Y,WU Y F.Development of a multiplex polymerase chain reaction for simultaneous detection of wheat viruses and a phytoplasma in China [J].Archives of Virology,2012,157(7):1263.
[3]于祥泉,陈旺,吴云锋,等.小麦黄矮病品种抗病性调查及种群多重PCR检测[J].麦类作物学报,2010,30(6):1054.YU X Q,CHEN W,WU Y F,et al.Investigation of wheat varieties on resistance to BYDV and detection of BYDV population by mutiplex PCR [J].Journal of Triticeae Crops,2010,30(6):1055.
[4]XIN Z Y,BRETTELL R,CHENG Z,et al.Characterization of a potential source of barley yellow dwarf virus resistance for wheat [J].Genome,188,30:252.
[5]SONG S,TAO Y,ZHANG H W,WU Y F.Psathyrostachys huashanica,a potential resource for resistance to barley yellow dwarf virus-GAV [J].European Journal of Plant Pathology,2013,137:218.
[6]ENDO R M.Hill technique for evaluating the resistance to BYDV [J].Phytopathology,1963,53:650.
[7]钱幼亭,周广和.麦类种质资源抗耐小麦黄矮病毒的田间鉴定技术研究[J].植物保护学报,1986,13(8):161.QIAN Y T,ZHOU G H.Studies on screening technique for wheat yellow dwarf virus in the field [J].Acta Phytophylacica Sinica,1986,13(8):161.
[8]JIN Z B,WANG X F,CHANG S J,et al.The complete nucleotide sequence and its organization of the genome of barley yellow dwarf virus-GAV [J].Science in China series C-life sciences,2004,47(2):178.
[9]SONG S,TAO Y,ZHAO L,et al.Isolation and characterization of complete genome of barley yellow dwarf virus-GAV from wheat in Northwestern China [J].Cereal Research Communications,2014,42(4):627.
[10]WU K,CHEN W,WANG B,et al.The distribution and molecular variability of four tobacco viruses in China [J].Virologica Sinica,2016,31(6):526.
[11]LI Q,HUANG J,HOU L,et al.Molecular mapping of stripe rust resistance gene in wheat-Psathyrostachys huashanica translocation line H9020-1-6-8-3 [J].Plant Disease,2012,96:1482.
[12]ZHANG Z Y,LIN Z S,XIN Z Y.Research progress in BYDV resistance genes derived from wheat and its wild relatives [J].Journal of Genetics and Genomics,2009,36(9):568.