小建中汤颗粒多波长切换液相色谱方法的含量测定研究
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摘要
目的:建立同时测定小建中汤颗粒中芍药苷、肉桂酸与甘草酸含量的方法。方法:采用多波长切换高效液相色谱法。色谱柱:Agilent C18(4.6 mm×250 mm,5μm);流动相:乙腈-0.1%磷酸梯度洗脱;检测波长:230 nm(芍药苷)、285 nm(肉桂酸)及254 nm(甘草酸);流速:1 mL/min;柱温:25℃。结果:芍药苷进样量在0.181～3.614μg(R2=0.9999)范围内线性关系良好,平均加样回收率为101.8%,RSD%=1.36%(n=6);肉桂酸进样量在0.005～0.101μg(R2=0.9999)范围内线性关系良好,平均加样回收率为97.14%,RSD%=2.87%(n=6);甘草酸进样量在0.038～0.768μg(R2=0.9999)范围内线性关系良好,平均加样回收率为101.4%,RSD%=1.73%(n=6)。结论:本方法操作简便、结果可靠、重复性好,可用于小建中汤颗粒的质量控制。
Objective:To establish a method for determination the content of paeoniflorin,cinnamic acid and glycyrrhizic acid in Xiaojianzhong tang granules.Methods:The method of multi wavelength switching HPLC was used.The column was Agilent C18(4.6 mm×250 mm,5 μm);mobile phase of acetonitrile-0.1% phosphoric acid,gradient elution;detection wavelength of 230 nm(paeoniflorin),285 nm(cinnamic acid) and 254 nm(glycyrrhizic acid);flow 1.0 mL/min;column temperature was 25 ℃.Results:Paeoniflorin injection volume within 0.181～3.614 μg(R2=0.9999) linear range,linear relationship was good,and the average recovery was 101.8%,RSD=1.36%(n=6);cinnamic acid injection volume within 0.005～0.101 μg(R2=0.999,9) linear range,linear relationship was good,and the average recovery was 97.14%,RSD=2.87%(n=6);glycyrrhizic acid injection volume within 0.038～0.768 μg(R2=0.999,9) linear range,linear relationship was good,and the average recovery was 101.4%,RSD=1.73%(n=6).Conclusion:This method is simple,reliable and reproducible,it can be used for quality control of Xiaojianzhong tang granules.
Objective:To establish a method for determination the content of paeoniflorin,cinnamic acid and glycyrrhizic acid in Xiaojianzhong tang granules.Methods:The method of multi wavelength switching HPLC was used.The column was Agilent C18(4.6 mm×250 mm,5 μm);mobile phase of acetonitrile-0.1% phosphoric acid,gradient elution;detection wavelength of 230 nm(paeoniflorin),285 nm(cinnamic acid) and 254 nm(glycyrrhizic acid);flow 1.0 mL/min;column temperature was 25 ℃.Results:Paeoniflorin injection volume within 0.181～3.614 μg(R2=0.9999) linear range,linear relationship was good,and the average recovery was 101.8%,RSD=1.36%(n=6);cinnamic acid injection volume within 0.005～0.101 μg(R2=0.999,9) linear range,linear relationship was good,and the average recovery was 97.14%,RSD=2.87%(n=6);glycyrrhizic acid injection volume within 0.038～0.768 μg(R2=0.999,9) linear range,linear relationship was good,and the average recovery was 101.4%,RSD=1.73%(n=6).Conclusion:This method is simple,reliable and reproducible,it can be used for quality control of Xiaojianzhong tang granules.
引文
[1]李飞.方剂学[M].2版.北京:人民卫生出版社,2015:655-659.
[2]李虹,黄立萍.小建中汤合膈下逐瘀汤治疗萎缩性胃炎50例[J].中国中医药科技,2012,19(5):467.
[3]杨梅.小建中汤合膈下逐瘀汤联合西药治疗慢性萎缩性胃炎随机平行对照研究[J].实用中医内科杂志,2013,27(7):127-128.
[4]张春蓉.小建中汤加白术治疗习惯性便秘20例[J].新中医,2004,36(2):61.
[5]谢言嵩.小建中汤治疗习惯性便秘[J].河南中医,2003,23(4):67.
[6]郑芳忠.小建中汤加味治疗腹泻型肠易激综合征72例[J].国医论坛,2011,26(5):10.
[7]杨军,樊春燕.小建中汤加减治疗肠易激综合征36例[J].陕西中医,2005,26(9):920.
[8]宋晓容.小建中汤联合匹维溴铵治疗脾虚型肠易激综合征33例[J].云南中医中药杂志,2013,34(6):37-38.
[9]奚胜艳,钱林超,钱小燕,等.加味小建中汤治疗消化性溃疡62例[J].中医杂志,2013,54(8):703-704.
[10]国家药典委员会.中国药典[S].北京:中国医药科技出版社,2015:572-573.
[11]陈巧谋,向莉琳,颜胜利,等.RP-HPLC法测定复方咳喘宁蜜炼膏中肉桂酸的含量[J].中医药导报,2008,14(12):65-66.
[12]陈海清.HPLC法同时测定小建中汤中肉桂酸和桂皮醛的含量[J].齐鲁药事,2012,31(9):527-528.
[13]勾晓丹,郑琰,刘淑敏.双波长HPLC法同时测定参芪健胃颗粒中芍药苷和阿魏酸的含量[J].中医药导报,2016,22(10):53-63.
[14]史培颖,林逢林,陈芳美,等.高效液相色谱法同时测定玉泉丸中的葛根素及甘草酸[J].中国医院药学杂志,2013,33(20):1657-1659.
[15]尹睿卓,王嘉仡,包永睿,等.山绿茶降压指纹图谱及一测多评法多指标成分定量研究[J].中国新药杂志,2015,24(15):132-137.
[2]李虹,黄立萍.小建中汤合膈下逐瘀汤治疗萎缩性胃炎50例[J].中国中医药科技,2012,19(5):467.
[3]杨梅.小建中汤合膈下逐瘀汤联合西药治疗慢性萎缩性胃炎随机平行对照研究[J].实用中医内科杂志,2013,27(7):127-128.
[4]张春蓉.小建中汤加白术治疗习惯性便秘20例[J].新中医,2004,36(2):61.
[5]谢言嵩.小建中汤治疗习惯性便秘[J].河南中医,2003,23(4):67.
[6]郑芳忠.小建中汤加味治疗腹泻型肠易激综合征72例[J].国医论坛,2011,26(5):10.
[7]杨军,樊春燕.小建中汤加减治疗肠易激综合征36例[J].陕西中医,2005,26(9):920.
[8]宋晓容.小建中汤联合匹维溴铵治疗脾虚型肠易激综合征33例[J].云南中医中药杂志,2013,34(6):37-38.
[9]奚胜艳,钱林超,钱小燕,等.加味小建中汤治疗消化性溃疡62例[J].中医杂志,2013,54(8):703-704.
[10]国家药典委员会.中国药典[S].北京:中国医药科技出版社,2015:572-573.
[11]陈巧谋,向莉琳,颜胜利,等.RP-HPLC法测定复方咳喘宁蜜炼膏中肉桂酸的含量[J].中医药导报,2008,14(12):65-66.
[12]陈海清.HPLC法同时测定小建中汤中肉桂酸和桂皮醛的含量[J].齐鲁药事,2012,31(9):527-528.
[13]勾晓丹,郑琰,刘淑敏.双波长HPLC法同时测定参芪健胃颗粒中芍药苷和阿魏酸的含量[J].中医药导报,2016,22(10):53-63.
[14]史培颖,林逢林,陈芳美,等.高效液相色谱法同时测定玉泉丸中的葛根素及甘草酸[J].中国医院药学杂志,2013,33(20):1657-1659.
[15]尹睿卓,王嘉仡,包永睿,等.山绿茶降压指纹图谱及一测多评法多指标成分定量研究[J].中国新药杂志,2015,24(15):132-137.