神经胶质瘤中神经鞘氨醇蛋白2的表达及意义
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摘要
目的:检测神经胶质瘤中神经鞘氨醇蛋白2(SPHK2)的表达水平以及在胶质瘤中的作用。方法:收集2017年1月至2017年12月深圳市第二人民医院神经外科手术切除的50例脑胶质瘤组织标本和10例外伤对照组脑组织,采用免疫组织化学方法检测神经胶质瘤组织及对照组脑组织中SPHK2、Ki–67的表达水平,分析SPHK2的表达水平与胶质瘤增殖的关系。结果:与对照组脑组织比较,神经胶质瘤中SPHK2、Ki–67表达水平明显增高,且SPHK2与Ki–67之间的差异有统计学意义(P <0.05)。结论:神经胶质瘤中SPHK2表达上调,可作为反映神经胶质瘤病理学特点的指标;另外数据表明SPHK2可能起到促进神经胶质瘤增殖的作用。
Objective To detect the expression level of sphingosine 2(SPHK2) in glioma and its role in glioma. Methods 50 glioma tissues and 10 control brain tissues were collected from January 2017 to December 2017 in Shenzhen second people's hospital.Immunohistochemistry was used to detect SPHK2 and Ki-67 in glioma tissues and control brain tissues. The relationship between SPHK2 and Ki–67 was also analyzed. Results Compared with the control group, the expression levels of SPHK2 and Ki-67 in gliomas were significantly increased, and the expression levels of SPHK2 was partly correlated with the expression levels of Ki-67(P < 0.05). Conclusion The expression of SPHK2 is up-regulated in glioma, which can be used as an indicator to reflect the pathological features of glioma; in addition, SPHK2 may play a role in promoting the proliferation of glioma.
Objective To detect the expression level of sphingosine 2(SPHK2) in glioma and its role in glioma. Methods 50 glioma tissues and 10 control brain tissues were collected from January 2017 to December 2017 in Shenzhen second people's hospital.Immunohistochemistry was used to detect SPHK2 and Ki-67 in glioma tissues and control brain tissues. The relationship between SPHK2 and Ki–67 was also analyzed. Results Compared with the control group, the expression levels of SPHK2 and Ki-67 in gliomas were significantly increased, and the expression levels of SPHK2 was partly correlated with the expression levels of Ki-67(P < 0.05). Conclusion The expression of SPHK2 is up-regulated in glioma, which can be used as an indicator to reflect the pathological features of glioma; in addition, SPHK2 may play a role in promoting the proliferation of glioma.
引文
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[2]Rafay A,Oborski MJ,Misun H,et al.Malignant gliomas:current perspectives in diagnosis,treatment,and early response assessment using advanced quantitative imaging methods[J].Cancer Management&Research,2014,6(1):149-170.
[3]Clarke J,Butowski N,Chang S.Recent advances in therapy for glioblastoma[J].Archives of Neurology,2010,67(3):279.
[4]Mahajan-Thakur S,Bien-Moller S,Marx S,et al.Sphingosine1-phosphate(S1P)signaling in glioblastoma multiforme-Asystematic review[J].International Journal of Molecular Sciences,2017,18(11):2448.
[5]Hatoum D,Haddadi N,Lin Y,et al.Mammalian sphingosine kinase(SphK)isoenzymes and isoform expression:challenges for SphK as an oncotarget[J].Oncotarget,2017,8(22):36898-36929.
[6]Dimasi DP,Pitson SM,Bonder CS.Examining the role of sphingosine kinase-2 in the regulation of endothelial cell barrier integrity[J].Microcirculation,2016,23(3):248-265.
[7]Funaki M,Kitabayashi J,Shimakami T,et al.Peretinoin,an acyclic retinoid,inhibits hepatocarcinogenesis by suppressing sphingosine kinase 1 expression in vitro and in vivo[J].Scintific Reports,2017,7(1):16978.
[8]Mitchell C,Kapitonov D,Allegood JC.Targeting sphingosine kinase 1 inhibits Akt signaling,induces apoptosis,and suppresses growth of human glioblastoma cells and xenografts[J].Cancer Research,2009,69(17):6915-6923.
[9]Inwald EC,Klinkhammer-Schalke M,Hofstadter F,et al.Ki-67 is a prognostic parameter in breast cancer patients:results of a large population-based cohort of a cancer registry[J].Breast Cancer Research&Treatment,2013,139(2):539-552.
[10]Ma YL,Peng JY,Zhang P,et al.Immunohistochemical analysis revealed CD34 and Ki67 protein expression as significant prognostic factors in colorectal cancer[J].Medical Oncology,2010,27(2):304-309.
[11]Huma A,Zubair A,Hasan SH.Gliomas:correlation of histologic grade,Ki67 and p53 expression with patient survival[J].Asian Pac J Cancer Prev,2010,11(6):1637-1640.
[12]Qiu W,Yang Z,Fan Y,et al.MicroRNA-613 inhibits cell growth,migration and invasion of papillary thyroid carcinoma by regulating SphK2[J].Oncotarget,2016,7(26):39907-39915.
[13]Wen PY,Kesari S.Malignant Gliomas in Adults[J].New England Journal of Medicine,2008,359(5):492-507.
[14]Kajimoto T,Okada T,Miya S,et al.Ongoing activation of sphingosine 1-phosphate receptors mediates maturation of exosomal multivesicular endosomes[J].Nature Communications,2015,4(4):2712.
[15]Gourlay J,Morokoff AP,Luwor RB,et al.The emergent role of exosomes in glioma[J].Journal of Clinical Neuroscience,2017,35(8):13-23.
[16]Figueroa J,Phillips LM,Shahar T,et al.Exosomes from Glioma-Associated Mesenchymal Stem Cells Increase the Tumorigenicity of Glioma Stem-like Cells via Transfer of miR-1587[J].Cancer Research,2017,77(21):5808-5819.