鹿衔草多糖对小鼠免疫活性的影响
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摘要
目的研究鹿衔草多糖对小鼠免疫活性的影响。方法分别采用正常小鼠模型和免疫抑制小鼠模型,以生理盐水为空白对照组,香菇多糖为阳性对照组,灌服鹿衔草多糖,观察小鼠腹腔巨噬细胞吞噬功能的影响及溶血素和溶血空斑形成的影响。结果与空白对照组相比,鹿衔草多糖组可显著提高正常小鼠和免疫低下小鼠腹腔巨噬细胞对鸡红细胞的吞噬百分率和吞噬指数,促进溶血素和溶血空斑的形成,其中以0.1g·kg~(-1)鹿衔草多糖剂量组作用为最佳。结论实验研究表明鹿衔草多糖具有明显的免疫增强作用。
OBJECTIVE To study the influence of Pyrola calliantha H.Andres Polysaccharides on immune activity of mouse.METHODS Normal mouse and immunosuppressive mouse were used respectively.Saline was used as blank control group and lentinan was used as positive control group.Pyrola calliantha H.Andres Polysaccharides was administered to observe the phagocytic function of peritoneal macrophages and the effect of hemolysin and hemolytic plaque formation in mouse RESULTS Compared with the blank control group,Pyrola calliantha H.Andres Polysaccharides could significantly increase the phagocytic percentage and phagocytic index of peritoneal macrophages of normal mouse and immunocompromised mouse to chicken red blood cells,and promote the formation of hemolysin and hemolytic plaque.Among them,Pyrola calliantha H.Andres Polysaccharides dosage group of 0.1 g·kg~(-1) had the best effect.CONCLUSION Pyrola calliantha H.Andres Polysaccharides have obviously effect of increase immunity.
OBJECTIVE To study the influence of Pyrola calliantha H.Andres Polysaccharides on immune activity of mouse.METHODS Normal mouse and immunosuppressive mouse were used respectively.Saline was used as blank control group and lentinan was used as positive control group.Pyrola calliantha H.Andres Polysaccharides was administered to observe the phagocytic function of peritoneal macrophages and the effect of hemolysin and hemolytic plaque formation in mouse RESULTS Compared with the blank control group,Pyrola calliantha H.Andres Polysaccharides could significantly increase the phagocytic percentage and phagocytic index of peritoneal macrophages of normal mouse and immunocompromised mouse to chicken red blood cells,and promote the formation of hemolysin and hemolytic plaque.Among them,Pyrola calliantha H.Andres Polysaccharides dosage group of 0.1 g·kg~(-1) had the best effect.CONCLUSION Pyrola calliantha H.Andres Polysaccharides have obviously effect of increase immunity.
引文
[1]国家药典委员会编.中国药典(2010版一部)[S].北京:中国医药科技出版社,2010,304.
[2]王西发,张健民.鹿蹄草有效成分的研究[J].中草药,1988,19(1):8-10.
[3]罗定强,杨燕子,宋莉,等.中国特有鹿蹄草属植物的研究进展[J].中草药,2004,35(4):107-110.
[4]盛华刚.鹿衔草的化学成分与药理作用研究进展[J].西北药学杂志,2012,27(4):383-385.
[5]丁存晶,刘俊田,王军宪,等.鹿衔草总黄酮对大鼠急性心肌缺血的保护作用[J].中药材,2007,30(9):1105-1109.
[6]王庭欣,王庭祥,吴广臣,等.黄芪多糖增强小鼠免疫功能的实验研究[J].时珍国医国药,2009,20(7):1763-1764.
[7]邢福国,王海霞,韩春超,等.鸡腿菇多糖免疫活性的初步研究[J].食品科学,2003,24(6):139-141.
[8]李晓坤,张华锋,董晶晶,等.首乌藤多糖对小鼠免疫功能的影响[A].第十一届全国青年药学工作者最新科研成果交流会论文集[C].杭州:中国药学会,2012.
[9]徐小华,郝鹏飞,王启帅,等.墨旱莲多糖对小鼠免疫功能的影响[A].第十届全国中药和天然药物学术研讨会论文集[C].洛阳:中国药学会,2009.
[10]齐丽娟,宋雁,王伟,等.用环磷酰胺建立小鼠免疫抑制动物模型[J].卫生研究,2010,39(3):313-315.
[11]彭勃,苗明三,郭会军,等.扶正排毒片干预环磷酰胺致免疫抑制小鼠免疫功能的效应[J].中国临床康复,2006,10(43):126-128.
[2]王西发,张健民.鹿蹄草有效成分的研究[J].中草药,1988,19(1):8-10.
[3]罗定强,杨燕子,宋莉,等.中国特有鹿蹄草属植物的研究进展[J].中草药,2004,35(4):107-110.
[4]盛华刚.鹿衔草的化学成分与药理作用研究进展[J].西北药学杂志,2012,27(4):383-385.
[5]丁存晶,刘俊田,王军宪,等.鹿衔草总黄酮对大鼠急性心肌缺血的保护作用[J].中药材,2007,30(9):1105-1109.
[6]王庭欣,王庭祥,吴广臣,等.黄芪多糖增强小鼠免疫功能的实验研究[J].时珍国医国药,2009,20(7):1763-1764.
[7]邢福国,王海霞,韩春超,等.鸡腿菇多糖免疫活性的初步研究[J].食品科学,2003,24(6):139-141.
[8]李晓坤,张华锋,董晶晶,等.首乌藤多糖对小鼠免疫功能的影响[A].第十一届全国青年药学工作者最新科研成果交流会论文集[C].杭州:中国药学会,2012.
[9]徐小华,郝鹏飞,王启帅,等.墨旱莲多糖对小鼠免疫功能的影响[A].第十届全国中药和天然药物学术研讨会论文集[C].洛阳:中国药学会,2009.
[10]齐丽娟,宋雁,王伟,等.用环磷酰胺建立小鼠免疫抑制动物模型[J].卫生研究,2010,39(3):313-315.
[11]彭勃,苗明三,郭会军,等.扶正排毒片干预环磷酰胺致免疫抑制小鼠免疫功能的效应[J].中国临床康复,2006,10(43):126-128.