GC-MS研究含天然麝香小金丸与含人工麝香小金丸特征化学成分
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摘要
目的:建立GC-MS法分析小金丸中的特征成分。方法:采用无水乙醇超声提取小金丸中的挥发性成分,再采用气相色谱-质谱联用技术和自动质谱解卷积定性系统(AMDIS)对其特征成分进行定性分析。结果:共鉴定出53个化学成分,含天然麝香小金丸中鉴定出30个化合物,而含人工麝香小金丸中鉴定出23个化合物。含天然麝香小金丸中检测出7个特征成分,分别是棕榈酸乙酯;乙酸脱氢异雄甾酮;3-乙基-3-羟基-雄甾烷-17-酮;去氢表雄酮;雄甾酮;3,17-二酮雄甾烷;3,17-二醇雄甾烷,其相对质量分数分别为0.11%,1.12%,2.80%,1.59%,0.56%,1.99%,1.22%。特征成分中的甾体类化合物来自于天然麝香,酯类化合物可能是天然麝香中的某些化合物与其他药物配伍反应生成的物质。结论:该方法特征性强、方法简便、重复性好,为区分含天然麝香小金丸和含人工麝香小金丸提供参考依据。
Objective: To establish the GC-MS analysis method for the identification of Xiaojin pills.Method: The volatile compounds in Xiaojin pills were extracted by ethanol,whose chemical constituents were then analyzed by gas chromatography-mass spectrum( GC-MS) and AMDIS. Result: A total of 53 compounds were identified,30 compounds were identified from Xiaojin pills containing natural moschus,23 compounds were identified from Xiaojin pills containing artificial musk. There are 7 characteristic compounds from Xiaojin pills containing natural musk,including palmitic acid ethyl ester,dehydroisoandrosterone 3-acetate,3-ethyl-3-hydroxyandrostan-17-one,dehydroepiandrosterone,androsterone,androstan-3,17-dione,androstane-3,17-diol,their relative contents were 0. 11%,1. 12%,2. 80%,1. 59%,0. 56%,1. 99% and 1. 22%. The steroidal compounds in the characteristic compounds are derived from natural musk,and the ester compound may be a substance produced by the compatibility of some compounds of natural musk with other drugs. Conclusion: This method is characteristic,simple and reproducible,which provides a reference for the distinction Xiaojin pills containing natural-musk and artificial-musk.
Objective: To establish the GC-MS analysis method for the identification of Xiaojin pills.Method: The volatile compounds in Xiaojin pills were extracted by ethanol,whose chemical constituents were then analyzed by gas chromatography-mass spectrum( GC-MS) and AMDIS. Result: A total of 53 compounds were identified,30 compounds were identified from Xiaojin pills containing natural moschus,23 compounds were identified from Xiaojin pills containing artificial musk. There are 7 characteristic compounds from Xiaojin pills containing natural musk,including palmitic acid ethyl ester,dehydroisoandrosterone 3-acetate,3-ethyl-3-hydroxyandrostan-17-one,dehydroepiandrosterone,androsterone,androstan-3,17-dione,androstane-3,17-diol,their relative contents were 0. 11%,1. 12%,2. 80%,1. 59%,0. 56%,1. 99% and 1. 22%. The steroidal compounds in the characteristic compounds are derived from natural musk,and the ester compound may be a substance produced by the compatibility of some compounds of natural musk with other drugs. Conclusion: This method is characteristic,simple and reproducible,which provides a reference for the distinction Xiaojin pills containing natural-musk and artificial-musk.
引文
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[2]秦树光.小金丸联合甲状腺素片治疗甲状腺肿的临床分析[J].现代中西医结合杂志,2005,14(8):1018.
[3]唐志强.小金丸治疗结节性甲状腺肿疗效观察[J].中国误诊学杂志,2007,7(8):1744-1745.
[4]屈江宁.小金丸治疗良性前列腺增生80例[J].世界中医药,2008,3(5):277.
[5]黄慧琳,曾幼波,林川,等.小金丸治疗乳腺增生的临床观察[J].海峡药学,2004,16(3):96-97.
[6]马保华,周彤.小金丸治疗带状疱疹后遗症神经痛44例[J].中国中医急症,2003,12(5):473-474.
[7]柳芳,鞠海,苗颖,等.小金丸及其组成药味抗肿瘤作用的研究进展[J].中国药房,2015,26(13):1844-1846.
[8]刘从盛.因为天然,所以珍贵—麝香与片仔癀[J].医药世界,2008,10(4):73.
[9]任桂林,韩丽,王小平,等.小金丸中制草乌单酯型生物碱类成分的含量测定[J].中国实验方剂学杂志,2014,20(21):123-126.
[10]平欲晖,毛红梅,关志宇,等.气相色谱法测定小金丸中麝香酮的含量研究[J].江西师范大学学报:自然科学版,2011,35(3):263-265.
[11]平欲晖,宗星星,关志宇,等.HPLC测定小金丸中乌头碱的含量[J].中国实验方剂学杂志,2013,19(9):80-81.
[12]刘刚,王辉,杨俊.高效液相色谱法测定小金丸中乌头碱的含量[J].广东药学院学报,2004,20(2):134-135.
[13]陈仁兴,王红霞.RP-HPLC法测定小金丸中阿魏酸的含量[J].中国医药指南,2003,11(17):501-502.
[14]王欣,刘新.高效液相色谱法测定小金丸软胶囊中阿魏酸的含量[J].中国医院药学杂志,2007,27(3):350-352.
[15]蔡博,董林毅,王静,等.超高效液相色谱质谱联用技术同时测定小金丸中7种成分的含量[J].中国药学杂志,2015,50(8):718-721.
[16]何玲玲.人工合成与天然麝香对中枢神经的影响研究[J].中国医药导报,2010,7(29):22-23.