基于气相色谱-质谱技术的小青龙汤证大鼠血液代谢组学研究
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摘要
目的采用气相色谱-质谱法(GC-MS)测定小青龙汤证大鼠血液中内源性代谢物,为小青龙汤证本质研究提供依据。方法将36只雄性Wistar大鼠随机分为空白对照组10只、模型组13只和小青龙汤组13只,模型组和小青龙汤组制作外寒内饮(小青龙汤证)模型,小青龙汤组灌胃小青龙汤,空白对照组不作任何处理。15 d后,3组大鼠腹主动脉取血,进行GC-MS检测。图谱经预处理后采用偏最小二乘法-判别分析进行多元统计分析。结果发现13种小分子标志物,分别为乳酸、3-羟基丁酸、甘氨酸、丝氨酸、赖氨酸、葡萄糖、花生四烯酸等代谢物质。结论乳酸、甘氨酸等标记物集中表现为能量代谢、免疫功能和炎症反应,为小青龙汤证本质研究提供了物质基础。
Objective To determine the endogenous metabolites in blood of rats with Xiaoqinglong Decoction Syndrome by using GC-MS;To provide evidence for researches on the essence of Xiaoqinglong Decoction Syndrome. Methods Totally 36 male Wistar rats were randomly divided into blank control group(10 rats), model group(13 rats), and Xiaoqinglong Decoction group(13 rats). Model group and Xiaoqinglong Decoction group were used to make the models of exogenous cold and internal fluid syndrome(Xiaoqinglong Decoction Syndrome). Rats in Xiaoqinglong Decoction group received gavage with Xiaoqinglong Decoction, while rats in blank control group received nothing. Blood of rats in the three groups was taken from the abdominal aorta and detected by GC-MS 15 days later, and analyzed by multivariate statistical analysis with PLS-DA. Results 13 kinds of biomarkers were found :lactic acid, 3-hydroxy-butyric acid, glycine, serine, lysine, dextrose, arachidonic acid and other metabolites. Conclusion Expressions of biomarkers, such as lactic acid and glycine, are energy metabolism, immune function, and inflammation, which provide material basis for researches on the essence of Xiaoqinglong Decoction Syndrome.
Objective To determine the endogenous metabolites in blood of rats with Xiaoqinglong Decoction Syndrome by using GC-MS;To provide evidence for researches on the essence of Xiaoqinglong Decoction Syndrome. Methods Totally 36 male Wistar rats were randomly divided into blank control group(10 rats), model group(13 rats), and Xiaoqinglong Decoction group(13 rats). Model group and Xiaoqinglong Decoction group were used to make the models of exogenous cold and internal fluid syndrome(Xiaoqinglong Decoction Syndrome). Rats in Xiaoqinglong Decoction group received gavage with Xiaoqinglong Decoction, while rats in blank control group received nothing. Blood of rats in the three groups was taken from the abdominal aorta and detected by GC-MS 15 days later, and analyzed by multivariate statistical analysis with PLS-DA. Results 13 kinds of biomarkers were found :lactic acid, 3-hydroxy-butyric acid, glycine, serine, lysine, dextrose, arachidonic acid and other metabolites. Conclusion Expressions of biomarkers, such as lactic acid and glycine, are energy metabolism, immune function, and inflammation, which provide material basis for researches on the essence of Xiaoqinglong Decoction Syndrome.
引文
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[3]赵龙山,李清,郭超伟,等.质谱联用技术在生物样品分析中的应用[J].药学学报,2012,47(2):158-162.
[4]盖江华,何丽清,郭鑫.基于核磁共振技术的小青龙汤证的证本质研究[J].世界中西医结合杂志,2013,8(3):230-233.
[5]夏鑫华,刘梅,李欢.基于衍生化反应和GC-MS技术测定大鼠血液和尿液中内源性代谢物轮廓谱[J].中华中医药学刊,2012,30(1):41-43.
[6]崔杰,郑兴宇,高晓霞,等.逍遥散抗抑郁作用最佳剂量筛选[J].中国实验方剂学杂志,2010,16(13):57-59
[7]林景超.化学性肝损伤及肝癌的代谢组学研究[D].上海:上海交通大学,2008.
[8]欧阳金生,刘少滨,黄绍光.慢性支气管炎大鼠模型白三烯B4水平的变化[J].中华结核和呼吸杂志,2006,29(5):343-344.
[9]刘芳英,申燕华,钱叶长.白三烯调节剂在支气管哮喘药物治疗中的研究进展[J].临床荟萃,2013,26(8):947-950.
[10]方芬,谭毅.咳嗽变异性哮喘与白三烯关系的研究进展[J].中国临床新医学,2013,6(1):87-90.