陆地棉光合和生理生化指标的耐盐性鉴定
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摘要
【目的】分析盐胁迫对陆地棉花铃期的光合和生理生化指标的影响,研究自然盐胁迫对陆地棉生理及产量的影响。建立综合得分(D值)公式,为棉花耐盐性的快速筛选和鉴定提供参考。【方法】试验设计采用随机区组方式,2个处理分别为盐胁迫和对照,并重复两次进行,试验地为自然盐碱地,盐含量是根据各区组五点采样法取40 cm处的土样,测得处理1(盐胁迫)总含盐量为19 g/kg,处理2(对照)总含盐量为4 g/kg。【结果】盐胁迫下光合指标明显下降,而不同的生理生化指标却有不同响应并且其相关性复杂;结合产量比较法得出盐胁迫下光合作用和POD活性越高,棉花品种越具有耐盐性,而MDA含量越低耐盐性越高。【结论】利用隶属函数法和主成分分析法建立得到公式,D=0.49F_1+0.16F_2+0.13F_3+0.12F_4+0.1F_5,可利用此公式筛选耐盐材料。
【Objective】 To explore the effects of salt stress on photosynthesis and physiochemical indexes of Gossypium hirsutum during the boll period, and to establish a comprehensive score(D value) formula, which might provide reference for rapid screening and identification of cotton salt tolerance.【Method】The whole experiment was designed in a randomized block mode. The two treatments were salt stress and control, and repeated twice. The other test sites were natural saline-alkali soils. The salt content was 40 cm according to the five-point sampling method of each block. At the soil sample, the total salt content of treatment 1(salt stress) was 19 g/kg, and the total salt content of treatment 2(control) was 4 g/kg. The effects of natural salt stress on the physiology and yield of gossypium hirsutum were studied.【Results】The results showed that the photosynthesis index decreased significantly under salt stress, but different physiological and biochemical indexes had different responses and their correlations were complex. The higher the photosynthesis and POD activity under salt stress were, the more salt tolerant cotton varieties were. The lower the MDA content, the higher the salt tolerance. The membership function method and principal component analysis method were used to establish the formula D=0.49F_1+0.16F_2+0.13F_3+0.12F_4+0.1F_5.【Conclusion】The comprehensive salt tolerance formula can be used to screen salt tolerant materials and used as a reference for rapid screening and identification of salt tolerance in cotton.
【Objective】 To explore the effects of salt stress on photosynthesis and physiochemical indexes of Gossypium hirsutum during the boll period, and to establish a comprehensive score(D value) formula, which might provide reference for rapid screening and identification of cotton salt tolerance.【Method】The whole experiment was designed in a randomized block mode. The two treatments were salt stress and control, and repeated twice. The other test sites were natural saline-alkali soils. The salt content was 40 cm according to the five-point sampling method of each block. At the soil sample, the total salt content of treatment 1(salt stress) was 19 g/kg, and the total salt content of treatment 2(control) was 4 g/kg. The effects of natural salt stress on the physiology and yield of gossypium hirsutum were studied.【Results】The results showed that the photosynthesis index decreased significantly under salt stress, but different physiological and biochemical indexes had different responses and their correlations were complex. The higher the photosynthesis and POD activity under salt stress were, the more salt tolerant cotton varieties were. The lower the MDA content, the higher the salt tolerance. The membership function method and principal component analysis method were used to establish the formula D=0.49F_1+0.16F_2+0.13F_3+0.12F_4+0.1F_5.【Conclusion】The comprehensive salt tolerance formula can be used to screen salt tolerant materials and used as a reference for rapid screening and identification of salt tolerance in cotton.
引文
[1]戴茂华,刘丽英,庞昭进,等.花铃期干旱胁迫对棉花生理生化指标的影响[J].江西农业学报,2015(7):19-21.DAI Mao-hua,LIU Li-ying,PANG Zhao-jin,et al.(2015).Effects of Drought Stress at Flowering and Boll-fo rming Stages on Physiological and Biochemical Indexes of Cotton[J].Acta Agriculturae Jiangxi,(7):19-21.(in Chinese)
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[5]王俊娟,叶武威,周大云,等.盐胁迫下不同耐盐类型棉花的萌发特性[J].棉花学报,2007,19(4):315-317WANG Jun-juan,YE Wu-wei,ZHOU Da-yun,et al.(2007).Studies on Germination Characteristics of Different Salinity-resistant Cotton under Salt Stress[J].Cotton Science,19(4):315-317.(in Chinese)
[6]孙小芳,刘友良,棉花品种耐盐性鉴定指标可靠性的检验[J].作物学报,2001,27(6):794-801SUN Xiao-fang,LIU You-liang.(2001).Test on Criteria of Evaluating Salt Tolerance of Cotton Cultivars[J].Acta Agronomica Sinica,27(6):794-801.(in Chinese)
[7]沈法富,尹承佾,于元杰,等.棉花植株和花粉耐盐性的鉴定,作物学报,1997(5):620-625SHEN Fa-fu,YIN Cheng-yu,YU Yuan-jie,et al.(1997).Identification of salt tolerance of cotton plants and pollen[J].Acta Agronomica Sinica,(5):620-625.(in Chinese)
[8]田又升,王志军,于航,等.干旱胁迫对不同抗旱性棉花品种抗氧化酶活性及基因表达的影响[J].西北植物学报,2015,35(12):2 483-2 490.TIAN You-sheng,WANG Zhi-jun,YU Hang,et al.(2015).Response of Antioxidant Enzyme Activities and Gene Expression in Different Drought Resistance Cotton Varieties under Drought Stress[J].Acta Botanica Boreali-Occidentalia Sinica,35(12):2,483-2,490.(in Chinese)
[9]Miao,Y.,Lv,D.,Wang,P.,Wang,X.C.,Chen,J.,&Miao,C.,et al.(2006).An arabidopsis glutathione peroxidase functions as both a redox transducer and a scavenger in abscisic acid and drought stress responses.Plant Cell,18(10):2,749-2,766.
[10]Mc Kersie,&B.(1993).Superoxide dismutase enhances tolerance of freezing stress in transgenic alfalfa(medicago sativa l.).PLANT PHYSIOLOGY,103(4):1,155-1,163.
[11]Parida,A.K.,Das,A.B.,&Mittra,B.(2004).Effects of salt on growth,ion accumulation,photosynthesis and leaf anatomy of the mangrove,bruguiera parviflora.Trees,18(2):167-174.
[13]王玉才,张恒嘉,邓浩亮.不同生育期调亏滴灌对菘蓝光合特性及品质的影响[J].核农学报,2017,31(9):1 847-1855.WANG Yu-cai,ZHANG Heng-jia,DENG Hao-liang.(2017).Effect of Regulated Deficit Irrigation at Different Growth Stages on Photosynthetic Characteristicsand Fruit Quality of Isatis[J].Acta Agriculturae Nucleatae Sinica,31(9):1,847-1,855.(in Chinese)
[14]刁丰秋,章文华,刘友良.盐胁迫对大麦叶片类囊体膜组成和功能的影响[J].分子植物(英文版),1997(2):105-110.YAN Feng-qiu,ZHANG Wen-hua,LIUYou-liang.(1997).Effects of Salt Stress on the Composition and Function of Thylakoid Membrane in Barley Leaves[J].Molecular Plant,(2):105-110.(in Chinese)
[15]杨秀艳李焕勇朱建峰,等Na Cl胁迫下2种白刺光合特性适应性研究[J].核农学报,2017,31(10):2 047-2 054.YANG Xiu-feng,LI Huan-yong,ZHU Jian-feng,et al.(2017).Study on Photosynthetic Characteristics of Nitraria Under Na Cl Stress[J].Acta Agriculturae Nucleatae Sinica,31(10):2,047-2,054.(in Chinese)
[16]李学孚,倪智敏,吴月燕,等.盐胁迫对'鄞红'葡萄光合特性及叶片细胞结构的影响[J].生态学报,2015,35(13):4436-4 444.LI Xue-fuo,NI Zhi-min,WU Yue-yang,et al.(2015).Effects of salt stress on photosynthetic characteristics and leaf cell structure of'Yinhong'grape seedlings[J].Acta Ecologica Sinica,35(13):4,436-4,444.(in Chinese)
[17]常青山,张利霞,米银法,等.外源ALA对盐胁迫下夏枯草幼苗抗氧化能力与光合特性的影响[J].核农学报,2017,31(10):2 055-2 062.CHANG Qing-shan,ZHANG Li-xia,MI Yin-fa,et al.(2017).Effects of Exogenous ALA on Antioxidant Capacities and Photosynthetic Characteristics in Prunella vulgaris Seedlings under Salt Stress[J].Acta Agriculturae Nucleatae Sinica,31(10):2,055-2,062.(in Chinese)
[18]孙宝玉,韩广轩,陈亮,等.短期模拟增温对黄河三角洲滨海湿地芦苇光响应特征的影响[J].生态学报,2018,38(1):167-176.SUN Bao-yu,HAN Guang-xuan,CHEN Liang,et al.(2018).Effects of short-term simulated warming on light response characteristics of reeds in coastal wetlands of the Yellow River Delta[J].Acta Ecologica Sinica,38(1):167-176.(in Chinese)
[19]王佺珍,刘倩,高娅妮,等.植物对盐碱胁迫的响应机制研究进展[J].生态学报,2017,37(16):5 565-5 577.WANG Yu-zhen,LIU Qian,GAO Ya-ni,et al.(2017).Advances in response mechanisms of plants to saline-alkali stress[J].Acta Ecologica Sinica,37(16):5,565-5,577.(in Chinese)
[20]Kurban,H.,Saneoka,H.,Nehira,K.,Adilla,R.,Premachandra,G.S.,&Fujita,K.(1999).Effect of salinity on growth,photosynthesis and mineral composition in leguminous plant alhagi pseudoalhagi(bieb.).Soil Science&Plant Nutrition,45(4):851-862.
[21]冯佰利,高小丽,王长发,等.干旱条件下不同温型小麦叶片衰老与活性氧代谢特性的研究[J].中国生态农业学报,2005,13(4):74-76.FENG Yu-li,GAO Xiao-li,WANG Chang-fa,et al.(2005).Study on Leaf Senescence and Active Oxygen Metabolism of Different Temperature Type Wheat under Drought Condition[J].Chinese Journal of Eco-Agriculture,13(4):74-76.(in Chinese)
[22]土壤水分胁迫对燕麦叶片渗透调节物质含量的影响[J].西北植物学报,2009,29(7):1 432-1 436.LIU Jing-hui,ZHAO Hai-chao,REN Yong-feng,et al.(2009).Change of Osmotica in Oat Leaf under Soil Moisture Stress[J].Acta Botanica Boreali-Occidentalia Sinica,29(7):1,432-1,436.(in Chinese)
[23]李翠,赵伟洁,张大爱,等.水分胁迫对糜子物质生产及光合特性的影响[J].西北农林科技大学学报:自然科学版,2014,42(1):89-95.LI Cui,ZHAO Wei-jie,ZHANG Da-ai,et al.(2014).Effects of water stress on material production and photosynthetic characteristics of Gardenia[J].Journal of Northwest A&F University,42(1):89-95.(in Chinese)
[2]彭振,何守朴,孙君灵,等.陆地棉苗期耐盐性的高效鉴定方法[J].作物学报,2014,40(3):476-486.PENG Zhen,HE Shou-pu,SUN JUun-ling,et al.(2014).An Efficient Approach to Identify Salt Tolerance of Upland Cotton at Seedling Stage[J].Acta Agronomica Sinica,40(3):476-486.(in Chinese)
[3]张国伟,路海玲,周治国,棉花萌发期和苗期耐盐性及耐盐指标评价.中国棉花学会年会,2010.ZHANG Guo-wei,LU Hai-ling,ZHOU Zhi-guo,Evaluation of salt tolerance and salt tolerance in cotton germination and seedling Period:Annual Meeting of China Cotton Society,2010.(in Chinese)
[4]张国伟,路海玲,张蕾,等.棉花萌发期和苗期耐盐性评价及耐盐指标筛选[J],应用生态学报,2011,22(8):2 045-2053.ZHANG Guo-wei,LU Hai-ling.ZHANG Lei,et al.(2011).Salt tolerance evaluation of cotton(Gossypium hirsutum)at its germinating and seedling stages and selection of related indices[j].Chinese Journal of Applied Ecology,22(8):2,045-2.053.(in Chinese)
[5]王俊娟,叶武威,周大云,等.盐胁迫下不同耐盐类型棉花的萌发特性[J].棉花学报,2007,19(4):315-317WANG Jun-juan,YE Wu-wei,ZHOU Da-yun,et al.(2007).Studies on Germination Characteristics of Different Salinity-resistant Cotton under Salt Stress[J].Cotton Science,19(4):315-317.(in Chinese)
[6]孙小芳,刘友良,棉花品种耐盐性鉴定指标可靠性的检验[J].作物学报,2001,27(6):794-801SUN Xiao-fang,LIU You-liang.(2001).Test on Criteria of Evaluating Salt Tolerance of Cotton Cultivars[J].Acta Agronomica Sinica,27(6):794-801.(in Chinese)
[7]沈法富,尹承佾,于元杰,等.棉花植株和花粉耐盐性的鉴定,作物学报,1997(5):620-625SHEN Fa-fu,YIN Cheng-yu,YU Yuan-jie,et al.(1997).Identification of salt tolerance of cotton plants and pollen[J].Acta Agronomica Sinica,(5):620-625.(in Chinese)
[8]田又升,王志军,于航,等.干旱胁迫对不同抗旱性棉花品种抗氧化酶活性及基因表达的影响[J].西北植物学报,2015,35(12):2 483-2 490.TIAN You-sheng,WANG Zhi-jun,YU Hang,et al.(2015).Response of Antioxidant Enzyme Activities and Gene Expression in Different Drought Resistance Cotton Varieties under Drought Stress[J].Acta Botanica Boreali-Occidentalia Sinica,35(12):2,483-2,490.(in Chinese)
[9]Miao,Y.,Lv,D.,Wang,P.,Wang,X.C.,Chen,J.,&Miao,C.,et al.(2006).An arabidopsis glutathione peroxidase functions as both a redox transducer and a scavenger in abscisic acid and drought stress responses.Plant Cell,18(10):2,749-2,766.
[10]Mc Kersie,&B.(1993).Superoxide dismutase enhances tolerance of freezing stress in transgenic alfalfa(medicago sativa l.).PLANT PHYSIOLOGY,103(4):1,155-1,163.
[11]Parida,A.K.,Das,A.B.,&Mittra,B.(2004).Effects of salt on growth,ion accumulation,photosynthesis and leaf anatomy of the mangrove,bruguiera parviflora.Trees,18(2):167-174.
[13]王玉才,张恒嘉,邓浩亮.不同生育期调亏滴灌对菘蓝光合特性及品质的影响[J].核农学报,2017,31(9):1 847-1855.WANG Yu-cai,ZHANG Heng-jia,DENG Hao-liang.(2017).Effect of Regulated Deficit Irrigation at Different Growth Stages on Photosynthetic Characteristicsand Fruit Quality of Isatis[J].Acta Agriculturae Nucleatae Sinica,31(9):1,847-1,855.(in Chinese)
[14]刁丰秋,章文华,刘友良.盐胁迫对大麦叶片类囊体膜组成和功能的影响[J].分子植物(英文版),1997(2):105-110.YAN Feng-qiu,ZHANG Wen-hua,LIUYou-liang.(1997).Effects of Salt Stress on the Composition and Function of Thylakoid Membrane in Barley Leaves[J].Molecular Plant,(2):105-110.(in Chinese)
[15]杨秀艳李焕勇朱建峰,等Na Cl胁迫下2种白刺光合特性适应性研究[J].核农学报,2017,31(10):2 047-2 054.YANG Xiu-feng,LI Huan-yong,ZHU Jian-feng,et al.(2017).Study on Photosynthetic Characteristics of Nitraria Under Na Cl Stress[J].Acta Agriculturae Nucleatae Sinica,31(10):2,047-2,054.(in Chinese)
[16]李学孚,倪智敏,吴月燕,等.盐胁迫对'鄞红'葡萄光合特性及叶片细胞结构的影响[J].生态学报,2015,35(13):4436-4 444.LI Xue-fuo,NI Zhi-min,WU Yue-yang,et al.(2015).Effects of salt stress on photosynthetic characteristics and leaf cell structure of'Yinhong'grape seedlings[J].Acta Ecologica Sinica,35(13):4,436-4,444.(in Chinese)
[17]常青山,张利霞,米银法,等.外源ALA对盐胁迫下夏枯草幼苗抗氧化能力与光合特性的影响[J].核农学报,2017,31(10):2 055-2 062.CHANG Qing-shan,ZHANG Li-xia,MI Yin-fa,et al.(2017).Effects of Exogenous ALA on Antioxidant Capacities and Photosynthetic Characteristics in Prunella vulgaris Seedlings under Salt Stress[J].Acta Agriculturae Nucleatae Sinica,31(10):2,055-2,062.(in Chinese)
[18]孙宝玉,韩广轩,陈亮,等.短期模拟增温对黄河三角洲滨海湿地芦苇光响应特征的影响[J].生态学报,2018,38(1):167-176.SUN Bao-yu,HAN Guang-xuan,CHEN Liang,et al.(2018).Effects of short-term simulated warming on light response characteristics of reeds in coastal wetlands of the Yellow River Delta[J].Acta Ecologica Sinica,38(1):167-176.(in Chinese)
[19]王佺珍,刘倩,高娅妮,等.植物对盐碱胁迫的响应机制研究进展[J].生态学报,2017,37(16):5 565-5 577.WANG Yu-zhen,LIU Qian,GAO Ya-ni,et al.(2017).Advances in response mechanisms of plants to saline-alkali stress[J].Acta Ecologica Sinica,37(16):5,565-5,577.(in Chinese)
[20]Kurban,H.,Saneoka,H.,Nehira,K.,Adilla,R.,Premachandra,G.S.,&Fujita,K.(1999).Effect of salinity on growth,photosynthesis and mineral composition in leguminous plant alhagi pseudoalhagi(bieb.).Soil Science&Plant Nutrition,45(4):851-862.
[21]冯佰利,高小丽,王长发,等.干旱条件下不同温型小麦叶片衰老与活性氧代谢特性的研究[J].中国生态农业学报,2005,13(4):74-76.FENG Yu-li,GAO Xiao-li,WANG Chang-fa,et al.(2005).Study on Leaf Senescence and Active Oxygen Metabolism of Different Temperature Type Wheat under Drought Condition[J].Chinese Journal of Eco-Agriculture,13(4):74-76.(in Chinese)
[22]土壤水分胁迫对燕麦叶片渗透调节物质含量的影响[J].西北植物学报,2009,29(7):1 432-1 436.LIU Jing-hui,ZHAO Hai-chao,REN Yong-feng,et al.(2009).Change of Osmotica in Oat Leaf under Soil Moisture Stress[J].Acta Botanica Boreali-Occidentalia Sinica,29(7):1,432-1,436.(in Chinese)
[23]李翠,赵伟洁,张大爱,等.水分胁迫对糜子物质生产及光合特性的影响[J].西北农林科技大学学报:自然科学版,2014,42(1):89-95.LI Cui,ZHAO Wei-jie,ZHANG Da-ai,et al.(2014).Effects of water stress on material production and photosynthetic characteristics of Gardenia[J].Journal of Northwest A&F University,42(1):89-95.(in Chinese)