国内外小麦种质主要矿质元素含量的评价分析
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摘要
矿质元素在人类新陈代谢中发挥着重要作用,提高矿质元素含量是小麦品质育种主要目标之一。为了了解小麦种质籽粒矿质养分状况并为小麦品质改良提供优良育种材料,本研究采用原子吸收法对214份国内外小麦种质的Cu、Ca、Fe和Zn 4种矿质元素进行了测定、分析。结果表明,供试小麦种质的4种矿质元素存在着较丰富的遗传变异,其变异系数分布在31.05%～67.46%之间;中国不同地区小麦种质的矿质元素存在较大差异,其中,以山东、江苏、四川、河南等主产区的小麦种质矿质元素含量较高;4种矿质元素中除Ca外,其余3种元素间均呈极显著正相关,这对小麦矿质元素综合提升比较有利。研究也筛选出了每种矿质元素含量最高的部分小麦种质,在以后品质育种中应重视对这些种质的利用。
Mineral elements play an important role in human metabolism.Increasing the content of mineral elements is one of the main objectives of wheat quality breeding.In order to investigate the mineral content of wheat germplasm in China and provided the elite wheat germplasm for wheat quality breeding program,4 kinds of mineral elements,Cu,Ca,Fe and Zn,were measured and analyzed by atomic absorption spectrometry(AAS)of214 domestic and foreign wheat germplasms.Results showed that there is the great genetic variation in the mineral elements among the different wheat germplasms.The coefficient of variation was changed between 31.05%and67.46%.The great difference was also found for wheat germplasms from the different districts,of which the germplasms from Sandong Province,Jiangsu Province,Sichuan Province and Henan Province had the higher mineral elements content.The positive significant correlation was found among the four mineral elements except Ca element,which is very useful to the comprehensive improvement of mineral elements of wheat.In addition,the elite germplasm with the higher element content were also selected and its utilization should be emphasized in the future wheat breeding practice.
Mineral elements play an important role in human metabolism.Increasing the content of mineral elements is one of the main objectives of wheat quality breeding.In order to investigate the mineral content of wheat germplasm in China and provided the elite wheat germplasm for wheat quality breeding program,4 kinds of mineral elements,Cu,Ca,Fe and Zn,were measured and analyzed by atomic absorption spectrometry(AAS)of214 domestic and foreign wheat germplasms.Results showed that there is the great genetic variation in the mineral elements among the different wheat germplasms.The coefficient of variation was changed between 31.05%and67.46%.The great difference was also found for wheat germplasms from the different districts,of which the germplasms from Sandong Province,Jiangsu Province,Sichuan Province and Henan Province had the higher mineral elements content.The positive significant correlation was found among the four mineral elements except Ca element,which is very useful to the comprehensive improvement of mineral elements of wheat.In addition,the elite germplasm with the higher element content were also selected and its utilization should be emphasized in the future wheat breeding practice.
引文
Hao Z.,Tian J.C.,Sun Y.,and Jiang X.L.,2008,Correlation analysis between contents of Cu,Fe,Zn,Mn and pigmentation of testa in different color wheat,Zhongguo Liangyou Xuebao(Journal of the Chinese Cereals and Oils Association),23(3):12-16(郝志,田纪春,孙玉,姜小苓,2008,不同粒色小麦籽粒中铁锌铜锰含量及其与种皮色素的相关分析,中国粮油学报,23(3):12-16)
Jiang L.N.,Gao B.Z.,Hou F.,Li C.X.,and Wang Z.M.,2008Dynamic accumulation of Fe,Zn,Mn and Cu in grain of common wheat during grain-filling stage,Mailei Zuowu Xuebao(Journal of Triticeae Crops),28(2):301-306(姜丽娜,蒿宝珍,侯飞,李春喜,王志敏,2008,小麦籽粒灌浆期铁,锌,锰,铜积累动态的研究,麦类作物学报,28(2)301-306)
Jiang L.N.,Gao B.Z.,Zhang D.J.,Shao Y.,and Li C.X.,2010Genotypic and envir onmental differences in grain contents of Zn,Fe,Mn and Cu and how they relate to wheat yield,Zhongguo Shengtai Nongye Xuebao(Chinese Journal of E-co-Agriculture),18(5):982-987(姜丽娜,蒿宝珍,张黛静,邵云,李春喜,2010,小麦籽粒Zn、Fe、Mn、Cu含量的基因型和环境差异及与产量关系的研究,中国生态农业学报,18(5):982-987)
King J.C.,2002,Evaluating the impact of plant biofortification on human nutrition,J.Nutr.,132:511-513
Li C.Y.,Feng C.N.,Wang Y.L.,Zhang R.,Guo W.S.,Zhu X.K.,and Peng Y.X.,2007,Differences of mineral element compositions and their contents among different positions of wheat grains,Zhiwu Shenglixue Tongxun(Plant Physiology Communications),43(6):1077-1081(李春燕,封超年,王亚雷,张容,郭文善,朱新开,彭永欣,2007,小麦籽粒不同部位的矿质元素组成与其含量差异,植物生理学通讯,43(6):1077-1081)
Li H.Y.,Wang S.X.,Li M.,Tian X.H.,Zhao A.Q.,and Guo C.H.,2014,Effects of combined foliar Zn application with N or Punder different water and nitrogen managements on Zn nutritional quality of winter wheat,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),47(20):4016-4026(李宏云,王少霞,李萌,田霄鸿,赵爱青,国春慧,2014,不同水氮管理下锌与氮磷肥配合喷施对冬小麦锌营养品质的影响,中国农业科学,47(20):4016-4026)
Liu Q.,Tian X.,and Shi Y.X.,2016,Effects of Se application on Se accumulation and transformation and content of gross protein and mineral elements in wheat grain,Zuowu Xuebao(Acta Agronomica Sinica),42(5):778-783(刘庆,田侠,史衍玺,2016,施硒对小麦籽粒硒富集,转化及蛋白质与矿质元素含量的影响,作物学报,42(5):778-783)
Lonneral B.,2003,Genetically modified plants for improved trace element nutrition,J.Nutr.,133:1490-1493
Shi R.L.,Zhou C.Q.,Rui Y.K.,Zhang X.Y.,Xia X.P.,and Zhang F.S.,2009,Application of ICP-AES to detecting nutrients in grain of wheat core collection of China,Guangpuxue Yu Guanpu Fengxi(Spectroscopy and Spectral Analysis),29(4):1104-1107(石荣丽,邹春琴,芮玉奎,张学勇,夏晓平,张福锁,2009,ICP-AES测定中国小麦微核心种质库籽粒矿质养分含量,光谱学与光谱分析,29(4):1104-1107)
Song W.,Sun L.Z.,Zhao A.H.,and Liu Q.,2004,Nutrition qualitative analysis and verification of colorful wheat variant offsprings produced by exogenous DNA introduction,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica,24(6):966-970(宋伟,孙兰珍,赵爱红,刘青,2004,外源DNA导入普通小麦变异后代有色小麦的营养品质分析和验证,西北植物学报,24(6):966-970)
Sun X.Y.,Tian F.,Mi Y.,Mu Q.H.,Wang R.X.,Wang C.,Qi X.L.,Qian Z.G.,Wu K.,and Li S.S.,2016,Analysis of major mineral elements concentration in grain of wheat recomb nant inbred lines,Mailei Zuowu Xuebao(Journal of Triticeae Crops),36(7):872-877(孙宪印,田枫,米勇,牟秋焕王瑞霞,王超,亓晓蕾,钱兆国,吴科,李斯深,2016,小麦重组自交系群体籽粒主要矿质元素含量的分析,麦类作物学报,36(7):872-877)
Tan L.G.,Fu X.L.,Zhang X.,and Yuan F.,2005,Trace element Human health,environment pollution and global change Weiliang Yuansu Yu Jiankang Yanjiu(Studies of Trace Elements and Health),22(4):49-51(谭绿贵,傅先兰,张鑫,袁峰,2005,微量元素·人体健康·环境变化,微量元素与健康研究,22(4):49-51)
Welch R.M.,and Graham R.D.,2004,Breeding for micronutrients in staple food crops from a human nutrition perspective J.Exp.Bot.,55(396):353-364
Xia R.X.,Wei S.,Guo B.L.,and Huang D.M.,2017,Analysis of mineral element content of wheat in northern area of henan province,Henongxue Bao(Journal of Nuclear Agricultural Sciences),31(3):516-523(夏瑞雪,魏帅,郭波莉,曹冬梅2017,豫北地区小麦籽粒矿质元素含量分析,核农学报31(3):516-523)
Yang L.L.,Liu X.J.,Xu J.,and Mao R.Z.,2008,Progress in research of micronutrients content in wheat grain,Mailei Zuowu Xuebao(Journal of Triticeae Crops),28(6):1113-1117(杨莉琳,刘小京,徐进,毛任钊,2008,小麦籽粒微量元素含量的研究进展,麦类作物学报,28(6):1113-1117)
Zhang D.J.,Jiang L.N.,Zhang Z.J.,Shao Y.,and Li C.X.,2011,Application of ICP-MS/ICP-AES to detecting nutrition and heavy metal contents in grain of detached wheat spikes in vitro culture,Guangpuxue Yu Guanpu Fengxi(Spectroscopy and Spectral Analysis),31(7):1935-1938(张黛静,姜丽娜,张志娟,邵云,李春喜,2011,ICP-MS/ICP-AES测定小麦穗离体培养籽粒营养元素及重金属含量,光谱学与光谱分析,31(7):1935-1938)
Zhang M.Y.,Yang Y.H.,Feng C.N.,Guo W.S.,Li C.Y.,Zhu X.K.,and Peng Y.X.,2014,Responses of concentration of mineral elements to zinc biofortification in different wheat genotypes,Mailei Zuowu Xuebao(Journal of Triticeae Crops),34(4):489-494(张明艳,杨宜豪,封超年,郭文善,李春燕,朱新开,彭永欣,2014,小麦籽粒矿质元素的基因型差异及对锌强化的响应,麦类作物学报,34(4):489-494)
Jiang L.N.,Gao B.Z.,Hou F.,Li C.X.,and Wang Z.M.,2008Dynamic accumulation of Fe,Zn,Mn and Cu in grain of common wheat during grain-filling stage,Mailei Zuowu Xuebao(Journal of Triticeae Crops),28(2):301-306(姜丽娜,蒿宝珍,侯飞,李春喜,王志敏,2008,小麦籽粒灌浆期铁,锌,锰,铜积累动态的研究,麦类作物学报,28(2)301-306)
Jiang L.N.,Gao B.Z.,Zhang D.J.,Shao Y.,and Li C.X.,2010Genotypic and envir onmental differences in grain contents of Zn,Fe,Mn and Cu and how they relate to wheat yield,Zhongguo Shengtai Nongye Xuebao(Chinese Journal of E-co-Agriculture),18(5):982-987(姜丽娜,蒿宝珍,张黛静,邵云,李春喜,2010,小麦籽粒Zn、Fe、Mn、Cu含量的基因型和环境差异及与产量关系的研究,中国生态农业学报,18(5):982-987)
King J.C.,2002,Evaluating the impact of plant biofortification on human nutrition,J.Nutr.,132:511-513
Li C.Y.,Feng C.N.,Wang Y.L.,Zhang R.,Guo W.S.,Zhu X.K.,and Peng Y.X.,2007,Differences of mineral element compositions and their contents among different positions of wheat grains,Zhiwu Shenglixue Tongxun(Plant Physiology Communications),43(6):1077-1081(李春燕,封超年,王亚雷,张容,郭文善,朱新开,彭永欣,2007,小麦籽粒不同部位的矿质元素组成与其含量差异,植物生理学通讯,43(6):1077-1081)
Li H.Y.,Wang S.X.,Li M.,Tian X.H.,Zhao A.Q.,and Guo C.H.,2014,Effects of combined foliar Zn application with N or Punder different water and nitrogen managements on Zn nutritional quality of winter wheat,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),47(20):4016-4026(李宏云,王少霞,李萌,田霄鸿,赵爱青,国春慧,2014,不同水氮管理下锌与氮磷肥配合喷施对冬小麦锌营养品质的影响,中国农业科学,47(20):4016-4026)
Liu Q.,Tian X.,and Shi Y.X.,2016,Effects of Se application on Se accumulation and transformation and content of gross protein and mineral elements in wheat grain,Zuowu Xuebao(Acta Agronomica Sinica),42(5):778-783(刘庆,田侠,史衍玺,2016,施硒对小麦籽粒硒富集,转化及蛋白质与矿质元素含量的影响,作物学报,42(5):778-783)
Lonneral B.,2003,Genetically modified plants for improved trace element nutrition,J.Nutr.,133:1490-1493
Shi R.L.,Zhou C.Q.,Rui Y.K.,Zhang X.Y.,Xia X.P.,and Zhang F.S.,2009,Application of ICP-AES to detecting nutrients in grain of wheat core collection of China,Guangpuxue Yu Guanpu Fengxi(Spectroscopy and Spectral Analysis),29(4):1104-1107(石荣丽,邹春琴,芮玉奎,张学勇,夏晓平,张福锁,2009,ICP-AES测定中国小麦微核心种质库籽粒矿质养分含量,光谱学与光谱分析,29(4):1104-1107)
Song W.,Sun L.Z.,Zhao A.H.,and Liu Q.,2004,Nutrition qualitative analysis and verification of colorful wheat variant offsprings produced by exogenous DNA introduction,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica,24(6):966-970(宋伟,孙兰珍,赵爱红,刘青,2004,外源DNA导入普通小麦变异后代有色小麦的营养品质分析和验证,西北植物学报,24(6):966-970)
Sun X.Y.,Tian F.,Mi Y.,Mu Q.H.,Wang R.X.,Wang C.,Qi X.L.,Qian Z.G.,Wu K.,and Li S.S.,2016,Analysis of major mineral elements concentration in grain of wheat recomb nant inbred lines,Mailei Zuowu Xuebao(Journal of Triticeae Crops),36(7):872-877(孙宪印,田枫,米勇,牟秋焕王瑞霞,王超,亓晓蕾,钱兆国,吴科,李斯深,2016,小麦重组自交系群体籽粒主要矿质元素含量的分析,麦类作物学报,36(7):872-877)
Tan L.G.,Fu X.L.,Zhang X.,and Yuan F.,2005,Trace element Human health,environment pollution and global change Weiliang Yuansu Yu Jiankang Yanjiu(Studies of Trace Elements and Health),22(4):49-51(谭绿贵,傅先兰,张鑫,袁峰,2005,微量元素·人体健康·环境变化,微量元素与健康研究,22(4):49-51)
Welch R.M.,and Graham R.D.,2004,Breeding for micronutrients in staple food crops from a human nutrition perspective J.Exp.Bot.,55(396):353-364
Xia R.X.,Wei S.,Guo B.L.,and Huang D.M.,2017,Analysis of mineral element content of wheat in northern area of henan province,Henongxue Bao(Journal of Nuclear Agricultural Sciences),31(3):516-523(夏瑞雪,魏帅,郭波莉,曹冬梅2017,豫北地区小麦籽粒矿质元素含量分析,核农学报31(3):516-523)
Yang L.L.,Liu X.J.,Xu J.,and Mao R.Z.,2008,Progress in research of micronutrients content in wheat grain,Mailei Zuowu Xuebao(Journal of Triticeae Crops),28(6):1113-1117(杨莉琳,刘小京,徐进,毛任钊,2008,小麦籽粒微量元素含量的研究进展,麦类作物学报,28(6):1113-1117)
Zhang D.J.,Jiang L.N.,Zhang Z.J.,Shao Y.,and Li C.X.,2011,Application of ICP-MS/ICP-AES to detecting nutrition and heavy metal contents in grain of detached wheat spikes in vitro culture,Guangpuxue Yu Guanpu Fengxi(Spectroscopy and Spectral Analysis),31(7):1935-1938(张黛静,姜丽娜,张志娟,邵云,李春喜,2011,ICP-MS/ICP-AES测定小麦穗离体培养籽粒营养元素及重金属含量,光谱学与光谱分析,31(7):1935-1938)
Zhang M.Y.,Yang Y.H.,Feng C.N.,Guo W.S.,Li C.Y.,Zhu X.K.,and Peng Y.X.,2014,Responses of concentration of mineral elements to zinc biofortification in different wheat genotypes,Mailei Zuowu Xuebao(Journal of Triticeae Crops),34(4):489-494(张明艳,杨宜豪,封超年,郭文善,李春燕,朱新开,彭永欣,2014,小麦籽粒矿质元素的基因型差异及对锌强化的响应,麦类作物学报,34(4):489-494)