敦煌固本方对脾虚型肠易激综合征大鼠结肠黏膜肥大细胞、类胰蛋白酶以及5-羟色胺的影响
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摘要
目的:探讨敦煌固本方对脾虚型肠易激综合征(D-IBS)大鼠结肠黏膜肥大细胞、类胰蛋白酶以及5-羟色胺(5-HT)的影响。方法:采用束缚+灌番泻叶造D-IBS大鼠模型,分为对照组、模型组、阳性药物组、敦煌固本方低、高剂量组,ELISA实验检测各组大鼠血清中5-HT含量变化;免疫组化实验检测各组大鼠结肠组织中类胰蛋白酶表达变化;甲苯胺蓝染色观察各组大鼠结肠组织中肥大细胞数量变化。结果:与模型组比较,敦煌固本方高剂量组血清中5-HT含量显著降低(P<0.01),类胰蛋白酶含量、肥大细胞数量在敦煌固本方高剂量组结肠组织中显著降低(P<0.01)。结论:敦煌固本方可通过减少D-IBS大鼠结肠组织中肥大细胞数量,进而使结肠组织中类胰蛋白酶含量降低,并使D-IBS大鼠血清中5-HT含量降低,从而达到对D-IBS的治疗作用。
Objective: To investigate the effect of Dunhuang Guben Formula on colonic mucosal mast cells, tryptase and serotonin(5-hydroxytryptamine) in rats with spleen deficiency irritable bowel syndrome(D-IBS). Methods: The rat model of D-IBS was established by restraint+senna. The changes of serum 5-HT content in the control group, model group, positive drug group, Dunhuang Guben Formula low-dose group and high-dose group were detected by ELISA. Immunohistochemistry was used to detect the expression of tryptase in colon tissue of each group. Toluidine blue staining was used to observe the changes of mast cells in colon tissue of each group. Results: Compared with the model group, the content of 5-HT in the group of Dunhuang Guben Formula high dose group was significantly decreased(P<0.01), and the content of tryptase was significantly decreased in the colon tissue of Dunhuang Guben Formula high dose group(P<0.01). The number of mast cells was significantly decreased in the colon tissue of Dunhuang Guben Formula high-dose group(P<0.01). Conclusion: Dunhuang Guben Formula can reduce the content of tryptase in colon tissue by reducing the number of mast cells in colon tissue of D-IBS rats, and reduce the content of 5-HT in serum of D-IBS rats, which achieves the therapeutic effect of D-IBS.
Objective: To investigate the effect of Dunhuang Guben Formula on colonic mucosal mast cells, tryptase and serotonin(5-hydroxytryptamine) in rats with spleen deficiency irritable bowel syndrome(D-IBS). Methods: The rat model of D-IBS was established by restraint+senna. The changes of serum 5-HT content in the control group, model group, positive drug group, Dunhuang Guben Formula low-dose group and high-dose group were detected by ELISA. Immunohistochemistry was used to detect the expression of tryptase in colon tissue of each group. Toluidine blue staining was used to observe the changes of mast cells in colon tissue of each group. Results: Compared with the model group, the content of 5-HT in the group of Dunhuang Guben Formula high dose group was significantly decreased(P<0.01), and the content of tryptase was significantly decreased in the colon tissue of Dunhuang Guben Formula high dose group(P<0.01). The number of mast cells was significantly decreased in the colon tissue of Dunhuang Guben Formula high-dose group(P<0.01). Conclusion: Dunhuang Guben Formula can reduce the content of tryptase in colon tissue by reducing the number of mast cells in colon tissue of D-IBS rats, and reduce the content of 5-HT in serum of D-IBS rats, which achieves the therapeutic effect of D-IBS.
引文
[1]赵宏宇,陈飞雪,李延青.肠黏膜分泌物调节肠易激综合征发病机制的研究进展.国际消化病杂志,2012,32(5):280-283
[2]Doyle L A,Sepehr G J,Hamilton M J,et al.A clinicopathologic study of 24 cases of systemic mastocytosis involving the gastrointestinal tract and assessment of mucosal mast cell density in irritable bowel syndrome and asymptomatic patients.The American Journal of Surgical Pathology,2014,38(6):832-843
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[4]李兆申,詹丽杏,邹多武,等.肠易激综合征患者分泌5-羟色胺的肠嗜铬细胞形态及功能的改变.中华消化杂志,2004,24(2):94-97
[5]刘喜平.敦煌古医方的研究概况.中成药,2004,26(1):8-9
[6]吕林,唐旭东,王凤云,等.肠易激综合征从脾论治理论探讨.中华中医药杂志,2017,32(3):943-946
[7]王珮珊.肠易激综合征(IBS)中医辨证分型规律的研究.南京:南京中医药大学,2008
[8]Sun J,Wu X,Meng Y,et al.Electro-acupuncture decreases5-HT,CGRP and increases NPY in the brain-gut axis in two rat models of Diarrhea-predominant irritable bowel syndrome(D-IBS).BMC Complementary and Alternative Medicine,2015,15(1):1-7
[9]Barbara G,Stanghellini V,D E Giorgio R,et al.Activated mast cells in proximity to colonic nerves correlate with abdominal pain in irritable bowel syndrome.Gastroenterology,2004,126(3):693-702
[10]Akbar A,Yiangou Y,Facer P,et al.Increased capsaicin receptor TRPV1-expressing sensory fibres in irritable bowel syndrome and their correlation with abdominal pain.Gut,2008,57(7):923-929
[11]Barbara G,Stanghellini V,D E Giorgio R,et al.Functional gastrointestinal disorders and mast cells:Implications for therapy.Neurogastroenterology and Motility:The Official Journal of the European Gastrointestinal Motility Society,2006,18(1):6-17
[12]Cenac N,Andrews C N,Holzhausen M,et al.Role for protease activity in visceral pain in irritable bowel syndrome.The Journal of Clinical Investigation,2007,117(3):636-647
[13]陈小燕,周礼鲲,柯美云.5-HT受体激动剂和拮抗剂及其在功能性胃肠病中的应用.中国处方药,2005,44(11):43-45
[2]Doyle L A,Sepehr G J,Hamilton M J,et al.A clinicopathologic study of 24 cases of systemic mastocytosis involving the gastrointestinal tract and assessment of mucosal mast cell density in irritable bowel syndrome and asymptomatic patients.The American Journal of Surgical Pathology,2014,38(6):832-843
[3]Carroccio A,Brusca I,Mansueto P,et al.Fecal assays detect hypersensitivity to cow’s milk protein and gluten in adults with irritable bowel syndrome.Clinical Gastroenterology and Hepatology:the Official Clinical Practice Journal of the American Gastroenterological Association,2011,9(11):965-971
[4]李兆申,詹丽杏,邹多武,等.肠易激综合征患者分泌5-羟色胺的肠嗜铬细胞形态及功能的改变.中华消化杂志,2004,24(2):94-97
[5]刘喜平.敦煌古医方的研究概况.中成药,2004,26(1):8-9
[6]吕林,唐旭东,王凤云,等.肠易激综合征从脾论治理论探讨.中华中医药杂志,2017,32(3):943-946
[7]王珮珊.肠易激综合征(IBS)中医辨证分型规律的研究.南京:南京中医药大学,2008
[8]Sun J,Wu X,Meng Y,et al.Electro-acupuncture decreases5-HT,CGRP and increases NPY in the brain-gut axis in two rat models of Diarrhea-predominant irritable bowel syndrome(D-IBS).BMC Complementary and Alternative Medicine,2015,15(1):1-7
[9]Barbara G,Stanghellini V,D E Giorgio R,et al.Activated mast cells in proximity to colonic nerves correlate with abdominal pain in irritable bowel syndrome.Gastroenterology,2004,126(3):693-702
[10]Akbar A,Yiangou Y,Facer P,et al.Increased capsaicin receptor TRPV1-expressing sensory fibres in irritable bowel syndrome and their correlation with abdominal pain.Gut,2008,57(7):923-929
[11]Barbara G,Stanghellini V,D E Giorgio R,et al.Functional gastrointestinal disorders and mast cells:Implications for therapy.Neurogastroenterology and Motility:The Official Journal of the European Gastrointestinal Motility Society,2006,18(1):6-17
[12]Cenac N,Andrews C N,Holzhausen M,et al.Role for protease activity in visceral pain in irritable bowel syndrome.The Journal of Clinical Investigation,2007,117(3):636-647
[13]陈小燕,周礼鲲,柯美云.5-HT受体激动剂和拮抗剂及其在功能性胃肠病中的应用.中国处方药,2005,44(11):43-45