牛奶菜水提物对中华眼镜蛇毒神经毒性的抑制作用
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摘要
【目的】考察牛奶菜(Marsdenia sinensis)水提物对中华眼镜蛇(Naja atra)毒神经毒性的抑制作用。【方法】利用四通道离体组织灌流系统和昆明小鼠(Mus musculus)离体回肠标本检测牛奶菜水提物对中华眼镜蛇毒神经毒性的抑制、即时中和与恢复作用。【结果】牛奶菜水提物对中华眼镜蛇毒神经毒性有一定抑制作用。【结论】研究结果揭示了牛奶菜用于治疗中华眼镜蛇咬伤的有效性,为后续开发抗蛇毒药物奠定了基础。
[Purposes]To investigate the inhibitory effect of Marsdenia sinensis aqueous extract on the neurotoxicity of Naja atra venom.[Methods]In vitro using mouse isolated ileum fragments to detect the inhibitions,immediate neutralizations and recovery effects of the aqueous extract from Chinese herb M.sinensis against the neurotoxicity of N.atravenom.[Findings]The aqueous extract from Chinese herb M.sinensis has a certain inhibitory effect against the neurotoxicity of N.atra venom.[Conclusions]The study revealed there is some effectiveness of Chinese herb M.sinensis used for the treatment of N.atra bites,also laid a base for the future studying on the anti-venom medicines.
[Purposes]To investigate the inhibitory effect of Marsdenia sinensis aqueous extract on the neurotoxicity of Naja atra venom.[Methods]In vitro using mouse isolated ileum fragments to detect the inhibitions,immediate neutralizations and recovery effects of the aqueous extract from Chinese herb M.sinensis against the neurotoxicity of N.atravenom.[Findings]The aqueous extract from Chinese herb M.sinensis has a certain inhibitory effect against the neurotoxicity of N.atra venom.[Conclusions]The study revealed there is some effectiveness of Chinese herb M.sinensis used for the treatment of N.atra bites,also laid a base for the future studying on the anti-venom medicines.
引文
[1]WHO.Snakebite envenoming[EB/OL].(2018-02-20)[2018-11-09].http://www.who.int/news-room/fact-sheets/detail/snakebite-envenoming.
[2]WHO.WHO highlights critical need for life-saving antivenoms[EB/OL].(2010-05-04)[2018-11-09].http://www.who.int/mediacentre/news/notes/2010/antivenoms_20100504/en/.
[3]王威,李其斌,陈泉芳.中华眼镜蛇咬伤中毒患者256例临床特点分析[J].山东医药,2012,52(33):46-48.WANG W,LI Q B,CHENG Q F.Analysis of clinical characteristics of 256cases of Naja atra bites poisoning patients[J].Shandong Medical Journal,2012,52(33):46-48.
[4]王威,李其斌.中华眼镜蛇咬伤的治疗[J].蛇志,2008,20(4):278-280.WANG W,LI Q B.Treatment of Naja atra bites[J].Journal of Snake,2008,20(4):278-280.
[5]王乃平,李其斌,李炳一,等.广西1990年蛇伤流行病学研究[J].蛇志,1993,5(1):10-17.WANG N P,LI Q B,LI B Y,et al.Epidemiological study on snake wounds in Guangxi in 1990[J].Journal of Snake,1993,5(1):10-17.
[6]张健,朱学.国内救治毒蛇咬伤17年回顾[J].蛇志,1994,6(3):31-35.ZHANG J,ZHU X.Chinese domestic treat snake bites 17-year review[J].Journal of Snake,1994,6(3):31-35.
[7]贾艳,胡延春,张乃生.蛇毒的毒性成分及其应用研究[J].蛇志,2004,16(2):23-32.JIA Y,HU Y C,ZHANG N S.Toxic components and application research of snake venom[J].Journal of Snake,2004,16(2):23-32.
[8]高忠恩,惠仲华.蝮蛇咬伤的中毒表现及中医辨证(附960例分析)[J].蛇志,1997,9(3):81-82.GAO Z E,HUI Z H.Poisoning performance of Agkistrodon bites and TCM syndrome differentiation(analysis of960cases)[J].Journal of Snake,1997,9(3):81-82.
[9]王威,陈泉芳,巫艳彬,等.广西境内两种常见神经毒类毒蛇咬伤中毒的临床特点与院前急救策略分析[J].中国全科医学,2014,17(14):1671-1673.WANG W,CHENG Q F,WU Y B,et al.Clinical characteristics of two common neurotoxic snake bites poisoning in Guangxi and analysis of pre-hospital emergency strategies[J].Chinese General Practice,2014,17(14):1671-1673.
[10]王威,李其斌,陈泉芳.广西境内两种竹叶青蛇咬伤中毒患者的临床特点分析[J].中国全科医学,2013,16(5C):1798-1800.WANG W,LI Q B,CHENG Q F.Analysis of clinical characteristics of two kinds of Trimeresurusstejnegeri bites poisoning patients in Guangxi[J].Chinese General Practice,2013,16(5C):1798-1800.
[11]宾文凯,邓立普,周克兵,等.急诊蛇伤患者的流行特征和救治研究[J].中国急救医学,2009,29(5):415-418.BING W K,DENG L P,ZHOU K B,et al.Emergency snakebite patient prevalent characteristics and clinical studies[J].Chinese Journal of Critical Care Medicine,2009,29(5):415-418.
[12]贺翠.整体护理在蛇咬伤患者中的应用[J].现代医学,2005,21(19):1589.HE C.Application of holistic nursing in patients with snake bites[J].Modern Medical Journal,2005,21(19):1589.
[13]和七一,余晓东,李博,等.小叶三点金醇提物对尖吻蝮蛇毒的抑制作用[J].中山大学学报(自然科学版),2018,57(4):128-135.HE Q Y,YU X D,LI B,et al.Inhibitory effects of ethanol extract of Desmodium microphyllum on Deinagkistrodon acutus venom[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2018,57(4):128-135.
[14]和七一,熊广均,李博,等.中草药徐长卿(Cynanchum paniculatum)治疗毒蛇咬伤的分子机制研究[J].中国科学:生命科学,2017,47(2):218-229.HE Q Y,XIONG G J,LI B,et al.Study on the molecular mechanism of Chinese herbal medicine Cynanchum paniculatum for treating snakebites[J].Scientia Sinica Vitae,2017,47(2):218-229.
[15]GOMES A,DAS R,SARKHEL S,et al.Herbs and herbal constituents active against snake bite[J].Indian Journal of Experimental Biology,2010,48(9):865-878.
[16]SOARES A M,TICLI F K,MARCUSSI S,et al.Medicinal plants with inhibitory properties against snake venom[J].Current Topics in Medicinal Chemistry,2005,12(22):2625-2641.
[17]ASSAFIM M,De CORIOLANO E C,BENEDITO S E,et al.Hypericum brasiliense plant extract neutralizes some biological effects of Bothrops jararaca snake venom[J].Joumal of Venom Research,2011,2(2):11-16.
[18]刘丽仙.对牛奶菜原植物描述的补充和修正[J].湖南中医药大学学报,1985(2):37-38.LIU L X.Supplements and corrections to the description of Marsdenia sinensis Hemsl original plants[J].Journal of Hunan University of Chinese Medicine,1985(2):37-38.
[19]中国科学院中国植物志编辑委员会.中国植物志[M].北京:科学出版社,1997.Editorial Committee of Flora of China of Chinese Academy of Sciences.Flora of China[M].Beijing:Science Press,1997.
[20]全国中草药汇编编写组.全国中草药汇编[M].北京:人民卫生出版社,1978.National Chinese Herbal Medicine Compilation Group.National Chinese herbal medicine compilation[M].Beijing:People’s Medical Publishing House(PMPH),1978.
[21]中国科学院植物研究所.中国高等植物图鉴第三册[M].北京:科学出版社,1974.Institute of Botany,Chinese Academy of Sciences.Iconographia cormophytorum sinicorum tomusⅢ[M].Beijing:Science Press,1974.
[22]酃县中药研究所.毛萝藦治疗毒蛇咬伤65例临床观察[J].湖南医药杂志,1981(6):11-12.Jixian Chinese Medicine Research Institute.Clinical observation of 65cases of snake bite treated with Maoluomo[J].Hunan Journal of Traditional Chinese Medicine,1981(6):11-12.
[23]TULADHAR B R,WOMACK M D,NAYLOR R J.Pharmacological characterization of the 5-HT receptormediated contraction in the mouse isolated ileum[J].British Journal of Pharmacology,2000,131(8):1716-1722.
[24]徐叔云,卞如濂,陈修.药理实验方法学[M].3版.北京:人民卫生出版社,2002.XU S Y,BIAN R L,CHEN X.Experimental methodology of pharmacology[M].3rd edition.Beijing:People’s Medical Publishing House,2002.
[25]邱泉.中草药在蛇伤防治中的应用进展[J].蛇志,2012,24(1):52-55.QIU Q.Progress in the application of Chinese herbal drug in the prevention and treatment of snake wounds[J].Journal of Snake,2012,24(1):52-55.
[26]KARLSSON E,AMBERG H,EAKER D.Isolation of the principal neurotoxins of two Naja naja subspecies[J].European Journal of Biochemistry,1971,21(1):1-16.
[27]EGGERTSEN P,LIND J,SJOQUIST J.Molecular characterization of the complement activating protein in the venom of the Indian cobra(Naja naja siamensis)[J].Molecular Immunology,1981,18(2):125-133.
[28]KUMAR T K,JAYARAMAN G,LEE C S,et al.Snake venom cardiotoxins-structure,dynamics,function and folding[J].Journal of Biomolecular Structure&Dynamics,1997,15:431-463.
[29]田文艺,兰芳,张昌平.毛萝藦注射液对蛇毒中毒动物保护作用的研究[J].湖南中医药大学学报,1985(1):54-55.TIAN W Y,LAN F,ZHANG C P.Study on the protective effect of Maoluomo injection on venom poisoning animals[J].Journal of Hunan University of Chinese Medicine,1985(1):54-55.
[30]黄石根.毛萝藦治疗毒蛇咬伤160例报告[J].中国临床医生杂志,1986(3):17-18.Huang S G.Report on 160cases of snake bites treated by Maoluomo[J].Chinese Journal for Clinicians,1986(3):17-18.
[31]熊广均,余晓东,熊艳,等.基于GC-MS对牛奶菜治疗蛇伤作用机制的初步研究[J].重庆师范大学学报(自然科学版),2019,36(1):89-95.XIONG G J,YU X D,XIONG Y,et al.Preliminary study of the therapeutic mechanism of Marsdenia sinensis on snakebite based on GC-MS[J].Journal of Chongqing Normal University(Natural Science),2019,36(1):89-95.
[2]WHO.WHO highlights critical need for life-saving antivenoms[EB/OL].(2010-05-04)[2018-11-09].http://www.who.int/mediacentre/news/notes/2010/antivenoms_20100504/en/.
[3]王威,李其斌,陈泉芳.中华眼镜蛇咬伤中毒患者256例临床特点分析[J].山东医药,2012,52(33):46-48.WANG W,LI Q B,CHENG Q F.Analysis of clinical characteristics of 256cases of Naja atra bites poisoning patients[J].Shandong Medical Journal,2012,52(33):46-48.
[4]王威,李其斌.中华眼镜蛇咬伤的治疗[J].蛇志,2008,20(4):278-280.WANG W,LI Q B.Treatment of Naja atra bites[J].Journal of Snake,2008,20(4):278-280.
[5]王乃平,李其斌,李炳一,等.广西1990年蛇伤流行病学研究[J].蛇志,1993,5(1):10-17.WANG N P,LI Q B,LI B Y,et al.Epidemiological study on snake wounds in Guangxi in 1990[J].Journal of Snake,1993,5(1):10-17.
[6]张健,朱学.国内救治毒蛇咬伤17年回顾[J].蛇志,1994,6(3):31-35.ZHANG J,ZHU X.Chinese domestic treat snake bites 17-year review[J].Journal of Snake,1994,6(3):31-35.
[7]贾艳,胡延春,张乃生.蛇毒的毒性成分及其应用研究[J].蛇志,2004,16(2):23-32.JIA Y,HU Y C,ZHANG N S.Toxic components and application research of snake venom[J].Journal of Snake,2004,16(2):23-32.
[8]高忠恩,惠仲华.蝮蛇咬伤的中毒表现及中医辨证(附960例分析)[J].蛇志,1997,9(3):81-82.GAO Z E,HUI Z H.Poisoning performance of Agkistrodon bites and TCM syndrome differentiation(analysis of960cases)[J].Journal of Snake,1997,9(3):81-82.
[9]王威,陈泉芳,巫艳彬,等.广西境内两种常见神经毒类毒蛇咬伤中毒的临床特点与院前急救策略分析[J].中国全科医学,2014,17(14):1671-1673.WANG W,CHENG Q F,WU Y B,et al.Clinical characteristics of two common neurotoxic snake bites poisoning in Guangxi and analysis of pre-hospital emergency strategies[J].Chinese General Practice,2014,17(14):1671-1673.
[10]王威,李其斌,陈泉芳.广西境内两种竹叶青蛇咬伤中毒患者的临床特点分析[J].中国全科医学,2013,16(5C):1798-1800.WANG W,LI Q B,CHENG Q F.Analysis of clinical characteristics of two kinds of Trimeresurusstejnegeri bites poisoning patients in Guangxi[J].Chinese General Practice,2013,16(5C):1798-1800.
[11]宾文凯,邓立普,周克兵,等.急诊蛇伤患者的流行特征和救治研究[J].中国急救医学,2009,29(5):415-418.BING W K,DENG L P,ZHOU K B,et al.Emergency snakebite patient prevalent characteristics and clinical studies[J].Chinese Journal of Critical Care Medicine,2009,29(5):415-418.
[12]贺翠.整体护理在蛇咬伤患者中的应用[J].现代医学,2005,21(19):1589.HE C.Application of holistic nursing in patients with snake bites[J].Modern Medical Journal,2005,21(19):1589.
[13]和七一,余晓东,李博,等.小叶三点金醇提物对尖吻蝮蛇毒的抑制作用[J].中山大学学报(自然科学版),2018,57(4):128-135.HE Q Y,YU X D,LI B,et al.Inhibitory effects of ethanol extract of Desmodium microphyllum on Deinagkistrodon acutus venom[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2018,57(4):128-135.
[14]和七一,熊广均,李博,等.中草药徐长卿(Cynanchum paniculatum)治疗毒蛇咬伤的分子机制研究[J].中国科学:生命科学,2017,47(2):218-229.HE Q Y,XIONG G J,LI B,et al.Study on the molecular mechanism of Chinese herbal medicine Cynanchum paniculatum for treating snakebites[J].Scientia Sinica Vitae,2017,47(2):218-229.
[15]GOMES A,DAS R,SARKHEL S,et al.Herbs and herbal constituents active against snake bite[J].Indian Journal of Experimental Biology,2010,48(9):865-878.
[16]SOARES A M,TICLI F K,MARCUSSI S,et al.Medicinal plants with inhibitory properties against snake venom[J].Current Topics in Medicinal Chemistry,2005,12(22):2625-2641.
[17]ASSAFIM M,De CORIOLANO E C,BENEDITO S E,et al.Hypericum brasiliense plant extract neutralizes some biological effects of Bothrops jararaca snake venom[J].Joumal of Venom Research,2011,2(2):11-16.
[18]刘丽仙.对牛奶菜原植物描述的补充和修正[J].湖南中医药大学学报,1985(2):37-38.LIU L X.Supplements and corrections to the description of Marsdenia sinensis Hemsl original plants[J].Journal of Hunan University of Chinese Medicine,1985(2):37-38.
[19]中国科学院中国植物志编辑委员会.中国植物志[M].北京:科学出版社,1997.Editorial Committee of Flora of China of Chinese Academy of Sciences.Flora of China[M].Beijing:Science Press,1997.
[20]全国中草药汇编编写组.全国中草药汇编[M].北京:人民卫生出版社,1978.National Chinese Herbal Medicine Compilation Group.National Chinese herbal medicine compilation[M].Beijing:People’s Medical Publishing House(PMPH),1978.
[21]中国科学院植物研究所.中国高等植物图鉴第三册[M].北京:科学出版社,1974.Institute of Botany,Chinese Academy of Sciences.Iconographia cormophytorum sinicorum tomusⅢ[M].Beijing:Science Press,1974.
[22]酃县中药研究所.毛萝藦治疗毒蛇咬伤65例临床观察[J].湖南医药杂志,1981(6):11-12.Jixian Chinese Medicine Research Institute.Clinical observation of 65cases of snake bite treated with Maoluomo[J].Hunan Journal of Traditional Chinese Medicine,1981(6):11-12.
[23]TULADHAR B R,WOMACK M D,NAYLOR R J.Pharmacological characterization of the 5-HT receptormediated contraction in the mouse isolated ileum[J].British Journal of Pharmacology,2000,131(8):1716-1722.
[24]徐叔云,卞如濂,陈修.药理实验方法学[M].3版.北京:人民卫生出版社,2002.XU S Y,BIAN R L,CHEN X.Experimental methodology of pharmacology[M].3rd edition.Beijing:People’s Medical Publishing House,2002.
[25]邱泉.中草药在蛇伤防治中的应用进展[J].蛇志,2012,24(1):52-55.QIU Q.Progress in the application of Chinese herbal drug in the prevention and treatment of snake wounds[J].Journal of Snake,2012,24(1):52-55.
[26]KARLSSON E,AMBERG H,EAKER D.Isolation of the principal neurotoxins of two Naja naja subspecies[J].European Journal of Biochemistry,1971,21(1):1-16.
[27]EGGERTSEN P,LIND J,SJOQUIST J.Molecular characterization of the complement activating protein in the venom of the Indian cobra(Naja naja siamensis)[J].Molecular Immunology,1981,18(2):125-133.
[28]KUMAR T K,JAYARAMAN G,LEE C S,et al.Snake venom cardiotoxins-structure,dynamics,function and folding[J].Journal of Biomolecular Structure&Dynamics,1997,15:431-463.
[29]田文艺,兰芳,张昌平.毛萝藦注射液对蛇毒中毒动物保护作用的研究[J].湖南中医药大学学报,1985(1):54-55.TIAN W Y,LAN F,ZHANG C P.Study on the protective effect of Maoluomo injection on venom poisoning animals[J].Journal of Hunan University of Chinese Medicine,1985(1):54-55.
[30]黄石根.毛萝藦治疗毒蛇咬伤160例报告[J].中国临床医生杂志,1986(3):17-18.Huang S G.Report on 160cases of snake bites treated by Maoluomo[J].Chinese Journal for Clinicians,1986(3):17-18.
[31]熊广均,余晓东,熊艳,等.基于GC-MS对牛奶菜治疗蛇伤作用机制的初步研究[J].重庆师范大学学报(自然科学版),2019,36(1):89-95.XIONG G J,YU X D,XIONG Y,et al.Preliminary study of the therapeutic mechanism of Marsdenia sinensis on snakebite based on GC-MS[J].Journal of Chongqing Normal University(Natural Science),2019,36(1):89-95.