摘要
细胞连接分子对上皮组织物质转运有重要的调节作用,其分子机制有待总结归纳。上皮组织中多数细胞连接分子参与形成紧密连接、粘附连接、桥粒连接和间隙连接等连接结构,调控细胞间转运和跨细胞运输。构成紧密连接的Claudin蛋白通过栅栏功能、受体功能和维持上皮细胞极性三种机制在调控上皮组织物质运输中起到至关重要的作用,其它细胞连接分子通过对紧密连接的调控间接影响物质运输。由于Claudin家族的各成员之间的功能代偿作用及转录后调控对Claudin蛋白的表达的重要性,给细胞连接研究造成一定局限,新的研究方法和技术有待发展。该文总结归纳出上皮细胞间连接分子调控物质转运的三种方式及当前应用于检测连接分子的生物技术,为基础和临床研究提供参考。
Cell junction molecules played important roles in regulating the transport of epithelialsubstances,and its molecular mechanisms had not been summarized yet. Most cell junction molecules in the epithelial tissues were involved in the formation of tight junctions,adhesion junctions,desmosome junctions,and gap junctions,regulating intercellular and transcellular transport. The Claudin proteins,which constituted tight junctions,played a crucial role in regulating the transport of epithelial substances through three mechanisms: barrier function,receptor function and maintaining the polarity of epithelial cells. Other cell junction molecules affected the substances transport indirectly through the regulation of tight junctions. Due to the compensatory functions of the members of the Claudin family and the importance of post-transcriptional regulation on the expression of Claudin proteins,the study of cell junctions had been limited,and new research methods and techniques needed to be developed. In this report,the three ways of regulating substance transport by cell junction molecules of epithelial cells and the biotechnology currently used to check the cell junction molecules had been summarized,providing a reference for basic and clinical research.
引文
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