咖啡树咖啡碱合成相关基因研究进展
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摘要
咖啡是世界三大饮料之一,其含有的咖啡碱,适量摄入对身体有益,过多则有害。因此,培育低咖啡碱或无咖啡碱咖啡品种具有非常重要的意义。目前,已从多数植物中分离并克隆出咖啡碱合成的相关基因,并研究了其表达特点和相关酶活性的调节机制,探讨培育低咖啡碱咖啡树的可能途径和存在的问题。综述近年来咖啡树咖啡碱合成与代谢的研究进展,展望如何培育出生长快、成本低、安全稳定的低咖啡碱植株,从而实现农业推广。
Coffee was one of the three major beverages in the world. Coffee contains caffeine, and is good to the health when taken adequately but harmful when taken too much. It is, therefore, very important to breed low caffeine or caffeine-free coffee varieties. The genes related to caffeine synthesis had been isolated and cloned from most of plants, and their expression and regulatory mechanisms underlying the activities of related enzymes had been analyzed. The problems and ways of breeding low caffeine or caffeine-free coffee varieties were discussed. The research progress of caffeine synthesis and metabolism in recent years were reviewed, based on which a prospect was made for breeding and extension of low caffeine coffee varieties with fast growth, low cost, safety and stability.
Coffee was one of the three major beverages in the world. Coffee contains caffeine, and is good to the health when taken adequately but harmful when taken too much. It is, therefore, very important to breed low caffeine or caffeine-free coffee varieties. The genes related to caffeine synthesis had been isolated and cloned from most of plants, and their expression and regulatory mechanisms underlying the activities of related enzymes had been analyzed. The problems and ways of breeding low caffeine or caffeine-free coffee varieties were discussed. The research progress of caffeine synthesis and metabolism in recent years were reviewed, based on which a prospect was made for breeding and extension of low caffeine coffee varieties with fast growth, low cost, safety and stability.
引文
[1]王华,海莲,何亚强,等.咖啡小粒种的主要品种来源及特性分析[J].中国农业信息,2014(9):89-90.
[2]李亚男,李荣福,黄家雄,等.咖啡主要栽培品种特性研究[J].安徽农业科学,2013,40(35):17038-17041.
[3]张箭.咖啡的起源、发展、传播及饮料文化初探[J].中国农史,2006,25(2):22-29.
[4]Wintgens J N,Wintgens J N.Coffee:growing,processing,sustainable production.A guidebook for growers,processors,traders and researchers[M].//Coffee:Growing,Processing,Sustainable Production:A Guidebook for Growers,Processors,Traders,and Researchers.2004:1966.
[5]Mazzafera P,Carvalho A.Breeding for low seed caffeine content of coffee(Coffea L.)by interspecific hybridization[J].Euphytica,1991,59(1):55-60.
[6]Zheng X,Ashihara H.Distribution,biosynthesis and function of purine and pyridine alkaloids in Coffea arabica seedlings[J].Plant Science,2004,166(3):807-813.
[7]姜美丽刘彩飞,巩荣,等.咖啡碱研究进展[J].农业工程技术·农产品加工业,2009(10):34-36.
[8]Perrois C,Strickler S R,Mathieu G,et al.Differential regulation of caffeine metabolism in Coffeaarabica(Arabica)and Coffea canephora(Robusta)[J].Planta,2015,241(1):179-191.
[9]Uefuji H,Ogita S,Yamaguchi Y,et al.Molecular cloning and functional characterization of three distinct N-methyltransferases involved in the caffeine biosynthetic pathway in coffee plants[J].Plant physiology,2003,132(1):372-380.
[10]Ogawa M,Herai Y,Koizumi N,et al.7-Methylxanthine Methyltransferase of Coffee Plants Gene Isolation and Enzymatic Properties[J].Journal of Biological Chemistry,2001,276(11):8 213-8 218.
[11]Mizuno K,Okuda A,Kato M,et al.Isolation of a new dual-functional caffeine synthase gene encoding an enzyme for the conversion of 7-methylxanthine to caffeine from coffee(Coffea arabica L.)[J].FEBS letters,2003,534(1):75-81.
[12]Smith A.Effects of caffeine on human behavior[J].Food and chemical toxicology,2002,40(9):1 243-1 255.
[13]Peeling P,Dawson B.Influence of caffeine ingestion on perceived mood states,concentration,and arousal levels during a 75-min university lecture[J].Advances in physiology education,2007,31(4):332-335.
[14]BrunyéT T,Mahoney C R,Lieberman H R,et al.Caffeine modulates attention network function[J].Brain and cognition,2010,72(2):181-188.
[15]Egawa T,Hamada T,Kameda N,et al.Caffeine acutely activates 5′adenosine monophosphate-activated protein kinase and increases insulin-independent glucose transport in rat skeletal muscles[J].Metabolism,2009,58(11):1 609-1 617.
[16]Bidel S,Hu G,Tuomilehto J.Coffee consumption and type 2 diabetes-An extensive review[J].Open Medicine,2008,3(1):9-19.
[17]Bairam A,Montandon G,Joseph V,et al.Enhancement of the breathing frequency response to hypoxia by neonatal caffeine treatment in adult male rats:The role of testosterone[J].Respira tory physiology&neurobiology,2009,165(2):261-265.
[18]Shilo L,Sabbah H,Hadari R,et al.The effects of coffee consumption on sleep and melatonin secretion[J].Sleep medicine,2002,3(3):271-273.
[19]Celik T,Iyisoy A,Amasyali B.The effects of coffee intake on coronary heart disease:Ongoing controversy[J].International journal of cardiology,2010,144(1):118.
[20]Montes F R,Cabrera M,Delgadillo A,et al.The role of potassium channels in the vasodilatory effect of caffeine in human internal mammary arteries[J].Vascular pharmacology,2009,50(3):132-136.
[21]Suzuki T,Ashihara H,Waller G R.Purine and purine alkaloid metabolism in Camellia and Coffea plants[J].Phytochemistry,1992,31(8):2 575-2584.
[22]Mccarthy A A,Mccarthy J G.The structure of two N-methyltransferases from the caffeine biosynthetic pathway[J].Plant physiology,2007,144(2):879-889.
[23]Mizuno K,Matsuzaki M,Kanazawa S,et al.Conversion of nicotinic acid to trigonelline is catalyzed by N-methyltransferase belonged to motif B′methyltransferase family in Coffea arabica[J].Biochemical and biophysical research communications,2014,452(4):1 060-1 066.
[24]Mohanan S,Gowda K,Kandukuri S V,et al.Involvement of a novel intronic microRNA in cross regulation of N-methyltransferase genes involved in caffeine biosynthesis in Coffea canephora[J].Gene,2013,519(1):107-112.
[25]Negishi O,Ozawa T,Imagawa H.N-methyl nucleosidase from tea leaves[J].Agricultural and biological chemistry,1988,52(1):169-175.
[26]Kato M,Mizuno K,Fujimura T,et al.Purification and characterization of caffeine synthase from tea leaves[J].Plant physiology,1999,120(2):579-586.
[27]Kato M,Mizuno K,Crozier A,et al.Plant biotechnology:Caffeine synthase gene from tea leaves[J].Nature,2000,406(6 799):956-957.
[28]Stasolla C,Katahira R,Thorpe T A,et al.Purine and pyrimidine nucleotide metabolism in higher plants[J].Journal of Plant Physiology.2003,160(11):1 271-1 295.
[29]冯艳飞,梁月荣.茶树S-腺苷甲硫氨酸合成酶基因的克隆和序列分析[J].茶叶科学,2001,21(1):21-25.
[30]Keya C A,Crozier A,Ashihara H.Inhibition of caffeine biosynthesis in tea(Camellia sinensis)and coffee(Coffea arabica)plants by ribavirin[J].FEBS letters,2003,554(3):473-477.
[31]Ogita S,Uefuji H,Morimoto M,et al.Application of RNAi to confirm theobromine as the major intermediate for caffeine biosynthesis in coffee plants with potential for construction of decaffeinated varieties[J].Plant molecular biology,2004,54(6):931-941.
[32]Denoeud F,Carretero P.L,Dereeper A,et al.The coffee genome provides insight into the convergent evolution of caffeine biosynthesis[J].science,2014,345(6 201):1 181-1 184.
[2]李亚男,李荣福,黄家雄,等.咖啡主要栽培品种特性研究[J].安徽农业科学,2013,40(35):17038-17041.
[3]张箭.咖啡的起源、发展、传播及饮料文化初探[J].中国农史,2006,25(2):22-29.
[4]Wintgens J N,Wintgens J N.Coffee:growing,processing,sustainable production.A guidebook for growers,processors,traders and researchers[M].//Coffee:Growing,Processing,Sustainable Production:A Guidebook for Growers,Processors,Traders,and Researchers.2004:1966.
[5]Mazzafera P,Carvalho A.Breeding for low seed caffeine content of coffee(Coffea L.)by interspecific hybridization[J].Euphytica,1991,59(1):55-60.
[6]Zheng X,Ashihara H.Distribution,biosynthesis and function of purine and pyridine alkaloids in Coffea arabica seedlings[J].Plant Science,2004,166(3):807-813.
[7]姜美丽刘彩飞,巩荣,等.咖啡碱研究进展[J].农业工程技术·农产品加工业,2009(10):34-36.
[8]Perrois C,Strickler S R,Mathieu G,et al.Differential regulation of caffeine metabolism in Coffeaarabica(Arabica)and Coffea canephora(Robusta)[J].Planta,2015,241(1):179-191.
[9]Uefuji H,Ogita S,Yamaguchi Y,et al.Molecular cloning and functional characterization of three distinct N-methyltransferases involved in the caffeine biosynthetic pathway in coffee plants[J].Plant physiology,2003,132(1):372-380.
[10]Ogawa M,Herai Y,Koizumi N,et al.7-Methylxanthine Methyltransferase of Coffee Plants Gene Isolation and Enzymatic Properties[J].Journal of Biological Chemistry,2001,276(11):8 213-8 218.
[11]Mizuno K,Okuda A,Kato M,et al.Isolation of a new dual-functional caffeine synthase gene encoding an enzyme for the conversion of 7-methylxanthine to caffeine from coffee(Coffea arabica L.)[J].FEBS letters,2003,534(1):75-81.
[12]Smith A.Effects of caffeine on human behavior[J].Food and chemical toxicology,2002,40(9):1 243-1 255.
[13]Peeling P,Dawson B.Influence of caffeine ingestion on perceived mood states,concentration,and arousal levels during a 75-min university lecture[J].Advances in physiology education,2007,31(4):332-335.
[14]BrunyéT T,Mahoney C R,Lieberman H R,et al.Caffeine modulates attention network function[J].Brain and cognition,2010,72(2):181-188.
[15]Egawa T,Hamada T,Kameda N,et al.Caffeine acutely activates 5′adenosine monophosphate-activated protein kinase and increases insulin-independent glucose transport in rat skeletal muscles[J].Metabolism,2009,58(11):1 609-1 617.
[16]Bidel S,Hu G,Tuomilehto J.Coffee consumption and type 2 diabetes-An extensive review[J].Open Medicine,2008,3(1):9-19.
[17]Bairam A,Montandon G,Joseph V,et al.Enhancement of the breathing frequency response to hypoxia by neonatal caffeine treatment in adult male rats:The role of testosterone[J].Respira tory physiology&neurobiology,2009,165(2):261-265.
[18]Shilo L,Sabbah H,Hadari R,et al.The effects of coffee consumption on sleep and melatonin secretion[J].Sleep medicine,2002,3(3):271-273.
[19]Celik T,Iyisoy A,Amasyali B.The effects of coffee intake on coronary heart disease:Ongoing controversy[J].International journal of cardiology,2010,144(1):118.
[20]Montes F R,Cabrera M,Delgadillo A,et al.The role of potassium channels in the vasodilatory effect of caffeine in human internal mammary arteries[J].Vascular pharmacology,2009,50(3):132-136.
[21]Suzuki T,Ashihara H,Waller G R.Purine and purine alkaloid metabolism in Camellia and Coffea plants[J].Phytochemistry,1992,31(8):2 575-2584.
[22]Mccarthy A A,Mccarthy J G.The structure of two N-methyltransferases from the caffeine biosynthetic pathway[J].Plant physiology,2007,144(2):879-889.
[23]Mizuno K,Matsuzaki M,Kanazawa S,et al.Conversion of nicotinic acid to trigonelline is catalyzed by N-methyltransferase belonged to motif B′methyltransferase family in Coffea arabica[J].Biochemical and biophysical research communications,2014,452(4):1 060-1 066.
[24]Mohanan S,Gowda K,Kandukuri S V,et al.Involvement of a novel intronic microRNA in cross regulation of N-methyltransferase genes involved in caffeine biosynthesis in Coffea canephora[J].Gene,2013,519(1):107-112.
[25]Negishi O,Ozawa T,Imagawa H.N-methyl nucleosidase from tea leaves[J].Agricultural and biological chemistry,1988,52(1):169-175.
[26]Kato M,Mizuno K,Fujimura T,et al.Purification and characterization of caffeine synthase from tea leaves[J].Plant physiology,1999,120(2):579-586.
[27]Kato M,Mizuno K,Crozier A,et al.Plant biotechnology:Caffeine synthase gene from tea leaves[J].Nature,2000,406(6 799):956-957.
[28]Stasolla C,Katahira R,Thorpe T A,et al.Purine and pyrimidine nucleotide metabolism in higher plants[J].Journal of Plant Physiology.2003,160(11):1 271-1 295.
[29]冯艳飞,梁月荣.茶树S-腺苷甲硫氨酸合成酶基因的克隆和序列分析[J].茶叶科学,2001,21(1):21-25.
[30]Keya C A,Crozier A,Ashihara H.Inhibition of caffeine biosynthesis in tea(Camellia sinensis)and coffee(Coffea arabica)plants by ribavirin[J].FEBS letters,2003,554(3):473-477.
[31]Ogita S,Uefuji H,Morimoto M,et al.Application of RNAi to confirm theobromine as the major intermediate for caffeine biosynthesis in coffee plants with potential for construction of decaffeinated varieties[J].Plant molecular biology,2004,54(6):931-941.
[32]Denoeud F,Carretero P.L,Dereeper A,et al.The coffee genome provides insight into the convergent evolution of caffeine biosynthesis[J].science,2014,345(6 201):1 181-1 184.