α-酮戊二酸对金针菇菌丝生长及赖氨酸合成的影响
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摘要
使用不同浓度的α-酮戊二酸处理金针菇(Flammulina filiformis)Dan3菌丝,结果显示低浓度α-酮戊二酸(1～5 mmol/L)促进菌丝生长,高浓度α-酮戊二酸(10～20 mmol/L)抑制其生长。金针菇赖氨酸合成途径中有3个基因(HCS、HIDH以及SDH)的表达模式呈α-酮戊二酸低浓度促进表达,而高浓度抑制表达;另有4个基因(HAH、AAR、AAT2和SDR)在添加α-酮戊二酸后表达量下降。金针菇菌丝中赖氨酸含量也显示出被低浓度α-酮戊二酸促进而被高浓度抑制的现象,2.5 mmol/L是诱导赖氨酸合成的最佳浓度。
The mycelia of F.filiformis Dan3 were treated with different concentrations of a-ketoglutaric acid.The results showed that the mycelia growth was accelerated by low concentrations of a-ketoglutaric acid(1 ～5 mmol/L)and inhibited by high concentrations(10 ～20 mmol/L).Further study showed that the expression levels of three genes(HCS,HIDH and SDH)in the lysine synthesis pathway of F.filiformis increased when the concentration of a-ketoglutaric acid was low,but decreased when the concentration of aketoglutaric acid was high.The expression levels of four other genes(HAH,AAR,AAT2 and SDR)decreased after adding α-ketoglutaric acid.The lysine content in F.filiformis showed the same trend as increased at low concentration of α-ketoglutaric acid and reduced at high concentration.2.5 mmol/L was the best concentration to induce lysine synthesis.
The mycelia of F.filiformis Dan3 were treated with different concentrations of a-ketoglutaric acid.The results showed that the mycelia growth was accelerated by low concentrations of a-ketoglutaric acid(1 ～5 mmol/L)and inhibited by high concentrations(10 ～20 mmol/L).Further study showed that the expression levels of three genes(HCS,HIDH and SDH)in the lysine synthesis pathway of F.filiformis increased when the concentration of a-ketoglutaric acid was low,but decreased when the concentration of aketoglutaric acid was high.The expression levels of four other genes(HAH,AAR,AAT2 and SDR)decreased after adding α-ketoglutaric acid.The lysine content in F.filiformis showed the same trend as increased at low concentration of α-ketoglutaric acid and reduced at high concentration.2.5 mmol/L was the best concentration to induce lysine synthesis.
引文
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[16]李莹,王志强,马超,等.外源α-酮戊二酸对干旱胁迫下小麦籽粒灌浆和产量形成的影响[J].麦类作物学报,2012,32(2):249-253.
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[19]蔡萌萌,刘子强,户红通,等.α-酮戊二酸对L-羟脯氨酸产量的影响[J].中国酿造,2018,37(7):47-50.
[20]LIU F,WANG W,CHEN BZ,et al. Homocitrate synthase expression and lysine content in fruiting body of different developmental stages in Flammulina velutipes[J].Current Microbiology,2015,70:821-828.
[2]戴玉成,杨祝良.中国五种重要食用菌学名新注[J].菌物学报,2018,37(12):1572-1577.
[3]袁仲.金针菇的保健作用与加工利用[J].农产品加工·学刊,2005(6-7):125-128.
[4]吴莹莹,鲍大鹏,王瑞娟,等.6种市售工厂化栽培金针菇的氨基酸组成及蛋白质营养评价[J].食品科学,2018,39(10):263-268.
[5]MALINOVSKY AV.Why Threonine is an essential amino acid in mammals and birds:Studies at the enzymatic level[J].Biochemistry,2018,83(7):795-799.
[6]罗中原,周梦舟,陈轩,等.糙米烘焙食品的研究现状与问题探讨[J].食品工业,2019,40(1):282-285.
[7]王镜岩,朱圣庚,徐长法.生物化学[M].北京:高等教育出版社,2002:346-347.
[8]KOSUGE T,HOSHINO T.Lysine is synthesized through the alpha-aminoadipate pathway in Thermus Thermophilus[J].FEMS Microbiology Letters,1998,169(2):361-367.
[9]BHATTACHARJEEA JK.alpha-Aminoadipate pathway for the biosynthesis of lysine in lower eukaryotes.[J].Critical Reviews in Microbiology,1985,12(2):131-151.
[10]XU HY,ANDI B,QIAN JH,et al.The alpha-aminoadipate pathway for lysine biosynthesis in fungi[J].Cell Biochemistry and Biophysics,2006,46(1):43-64.
[11]刘建雨,王瑞娟,张丹,等.基于金针菇全基因组的赖氨酸合成途径关键酶分析[J].微生物学通报,2016,43(10):2225-2233.
[12]汪虹,陈明杰.草菇冷诱导Cor3基因实时荧光定量PCR标准品质粒和标准曲线的构建[J].食用菌学报,2007,14(3):16-19.
[13]MORGUNOVG,KAMZOLOVA SV,SAMOILENKO VA.Enhancedα-ketoglutaric acid production and recovery in Yarrowia lipolytica yeast by effective pH controlling[J].Applied Microbiology and Biotechnology,2013,97(19):8711-8718.
[14]GOU L,LEE J,YANG JM,et al.The effect of alpha-ketoglutaric acid on tyrosinase activity and conformation:Kinetics and molecular dynamics simulation study[J].International Journal of Biological Macromolecules,2017,105:1654-1662.
[15]郭昊璐,于航宇,孙会.α-酮戊二酸对动物营养物质代谢和一些功能性指标的调控作用及其机理[J].动物营养学报,2018,30(5):1662-1667.
[16]李莹,王志强,马超,等.外源α-酮戊二酸对干旱胁迫下小麦籽粒灌浆和产量形成的影响[J].麦类作物学报,2012,32(2):249-253.
[17]BAYLIAK MM,HRYNKIV OV,KNYHYNYTSKA RV,et al.Alpha-ketoglutarate enhances freeze-thaw tolerance and prevents carbohydrate-induced cell death of the yeast Saccharomyces cerevisiae[J].Archives of Microbiology,2018,200(1):33-46.
[18]GRZESIAK P,SLUPECKA-ZIEMILSKA M,WOLINSKI J.The biological role of a-ketoglutaric acid in physiological processes and its therapeutic potential[J].Developmental Period Medicine,2016,20(1):61-67.
[19]蔡萌萌,刘子强,户红通,等.α-酮戊二酸对L-羟脯氨酸产量的影响[J].中国酿造,2018,37(7):47-50.
[20]LIU F,WANG W,CHEN BZ,et al. Homocitrate synthase expression and lysine content in fruiting body of different developmental stages in Flammulina velutipes[J].Current Microbiology,2015,70:821-828.