The Effect of Lignification Process on the Bioconversion Efficiency in Moso Bamboo
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摘要
Recalcitrance of lignocellulosic biomass is closely related to the presence of lignin in secondary cell walls, which has a negative effect on enzyme digestibility, biomass-to-biofuels conversion, and chemical pulping. The lignification process and structural heterogeneity of the cell wall for various parts of moso bamboo were investigated. There were slight differences among three different column parts of moso bamboo in terms of chemical compositions, including cellulose, hemicelluloses, and lignin. However, the detailed analysis of the fractionated lignin indicated that the acid-soluble lignin was first biosynthesized, and the largest molecular weight value was detected from the bottom part of the moso bamboo, as well as the highest syringyl-to-guaiacyl ratio. Although the main b-O-4 aryl ethers and resinol structures were clearly present in all lignin samples examined by NMR analysis, the relatively small lignin biomacromolecule in the top part of the moso bamboo lead to poor thermal stability. For the bioconversion process, no significant difference was found among all the moso bamboo samples, and the relatively higher hydrolysis efficiency was largely dependent on the low crystallinity of cellulose rather than the degree of lignin biosynthesis.
Recalcitrance of lignocellulosic biomass is closely related to the presence of lignin in secondary cell walls, which has a negative effect on enzyme digestibility, biomass-to-biofuels conversion, and chemical pulping. The lignification process and structural heterogeneity of the cell wall for various parts of moso bamboo were investigated. There were slight differences among three different column parts of moso bamboo in terms of chemical compositions, including cellulose, hemicelluloses, and lignin. However, the detailed analysis of the fractionated lignin indicated that the acid-soluble lignin was first biosynthesized, and the largest molecular weight value was detected from the bottom part of the moso bamboo, as well as the highest syringyl-to-guaiacyl ratio. Although the main b-O-4 aryl ethers and resinol structures were clearly present in all lignin samples examined by NMR analysis, the relatively small lignin biomacromolecule in the top part of the moso bamboo lead to poor thermal stability. For the bioconversion process, no significant difference was found among all the moso bamboo samples, and the relatively higher hydrolysis efficiency was largely dependent on the low crystallinity of cellulose rather than the degree of lignin biosynthesis.
Recalcitrance of lignocellulosic biomass is closely related to the presence of lignin in secondary cell walls, which has a negative effect on enzyme digestibility, biomass-to-biofuels conversion, and chemical pulping. The lignification process and structural heterogeneity of the cell wall for various parts of moso bamboo were investigated. There were slight differences among three different column parts of moso bamboo in terms of chemical compositions, including cellulose, hemicelluloses, and lignin. However, the detailed analysis of the fractionated lignin indicated that the acid-soluble lignin was first biosynthesized, and the largest molecular weight value was detected from the bottom part of the moso bamboo, as well as the highest syringyl-to-guaiacyl ratio. Although the main b-O-4 aryl ethers and resinol structures were clearly present in all lignin samples examined by NMR analysis, the relatively small lignin biomacromolecule in the top part of the moso bamboo lead to poor thermal stability. For the bioconversion process, no significant difference was found among all the moso bamboo samples, and the relatively higher hydrolysis efficiency was largely dependent on the low crystallinity of cellulose rather than the degree of lignin biosynthesis.
引文
[1]Ragauskas A J,Williams C K,Davison B H,et al.The path forward for biofuels and biomaterial[J].Science,2006,311(5760):484-489.
[2]Lu F,Ralph J.Solution-state NMR of lignocellulosic biomass[J].Journal of Biobased Materials&Bioenergy,2011,5(2):169-180.
[3]Suzuki K,Itoh T.The changes in cell wall architecture during lignification of bamboo,Phyllostachys aurea Carr.[J].Trees,2001,15(3):137-147.
[4]Mera F A T,Xu C.Plantation management and bamboo resource economics in China[J].Revista Ciencia YTecnología,2014,7(1):1-12.
[5]Song X.Observed high and persistent carbon uptake by Moso bamboo forests and its response to environmental drivers[J].Agricultural and Forest Meteorology,2017,247:467-475.
[6]Yang S M,Jiang Z H,Ren H Q,et al.Study status and development tendency of bamboo lignin[J].Wood Processing Machinery,2008,86(3):123-126.
[7]Yoshizawa N,Satoh I,Yokota S,et al.Lignification and peroxidase activity in bamboo shoots(Phyllostachys edulis A.et C.Riv.)[J].Holzforschung,1991,45:169-174.
[8]Itoh T.Lignification of bamboo(Phyllostachys heterocycla Mitf.)during its growth[J].Holzforschung,2009,44(3):191-200.
[9]Lybeer B,Koch G,Acker J V,et al.Lignification and cell wall thickening in nodes of Phyllostachys viridiglaucescens and Phyllostachys nigra[J].Annals of Botany,2006,97(4):529-539.
[10]Muhammad N,Man Z,Bustam M A,et al.Dissolution and delignification of bamboo biomass using amino acid-based ionic liquid[J].Applied Biochemistry&Biotechnology,2011,165(3/4):998-1009.
[11]Vu T M,Pakkanen H,Alen R.Delignification of bamboo(Bambusa procera acher):part 1.Kraft pulping and the subsequent oxygen delignification to pulp with a low Kappa number[J].Industrial Crops&Products,2004,19(1):49-57.
[12]Wen J L,Sun S L,Yuan T Q,et al.Structural elucidation of whole lignin from Eucalyptus based on preswelling and enzymatic hydrolysis[J].Green Chemistry,2015,17(3):1589-1596.
[13]Wang K,Xu F,Sun R C.Molecular characteristics of Kraft-AQ pulping lignin fractionated by sequential organic solvent extraction[J].International Journal of Molecular Sciences,2010,11:2988-3001.
[14]Vanholme R,Cesarino I,Rataj K,et al.Caffeoyl shikimate esterase(CSE)is an enzyme in the lignin biosynthetic pathway in Arabidopsis[J].Science,2013,341(6150):1103-1106.
[15]Wen J L,Sun S L,Xue B L,et al.Quantitative structural characterization of the lignins from the stem and pith of bamboo(Phyllostachys pubescens)[J].Holzforschung,2013,67(6):613-627.
[16]Kacurakova M,Capek P,Sasinkova V,et al.FT-IR study of plant cell wall model compounds:pectic polysaccharides and hemicelluloses[J].Carbohydrate Polymers,2000,43(2):195-203.
[17]Zhang X,Yu H,Huang H,et al.Evaluation of biological pretreatment with white rot fungi for the enzymatic hydrolysis of bamboo culms[J].International Biodeterioration&Biodegradation,2007,60(3):159-164.
[18]Ma X J,Cao S L,Yang X F,et al.Lignin removal and benzene-alcohol extraction effects on lignin measurements of the hydrothermal pretreated bamboo substrate[J].Bioresource Technology,2014,151C(1):244-248.
[19]Wang K,Yang H Y,Yao X,et al.Structural transformation of hemicelluloses and lignin from triploid poplar during acid-pretreatment based biorefinery process[J].Bioresource Technology,2012,116:99-106.
[2]Lu F,Ralph J.Solution-state NMR of lignocellulosic biomass[J].Journal of Biobased Materials&Bioenergy,2011,5(2):169-180.
[3]Suzuki K,Itoh T.The changes in cell wall architecture during lignification of bamboo,Phyllostachys aurea Carr.[J].Trees,2001,15(3):137-147.
[4]Mera F A T,Xu C.Plantation management and bamboo resource economics in China[J].Revista Ciencia YTecnología,2014,7(1):1-12.
[5]Song X.Observed high and persistent carbon uptake by Moso bamboo forests and its response to environmental drivers[J].Agricultural and Forest Meteorology,2017,247:467-475.
[6]Yang S M,Jiang Z H,Ren H Q,et al.Study status and development tendency of bamboo lignin[J].Wood Processing Machinery,2008,86(3):123-126.
[7]Yoshizawa N,Satoh I,Yokota S,et al.Lignification and peroxidase activity in bamboo shoots(Phyllostachys edulis A.et C.Riv.)[J].Holzforschung,1991,45:169-174.
[8]Itoh T.Lignification of bamboo(Phyllostachys heterocycla Mitf.)during its growth[J].Holzforschung,2009,44(3):191-200.
[9]Lybeer B,Koch G,Acker J V,et al.Lignification and cell wall thickening in nodes of Phyllostachys viridiglaucescens and Phyllostachys nigra[J].Annals of Botany,2006,97(4):529-539.
[10]Muhammad N,Man Z,Bustam M A,et al.Dissolution and delignification of bamboo biomass using amino acid-based ionic liquid[J].Applied Biochemistry&Biotechnology,2011,165(3/4):998-1009.
[11]Vu T M,Pakkanen H,Alen R.Delignification of bamboo(Bambusa procera acher):part 1.Kraft pulping and the subsequent oxygen delignification to pulp with a low Kappa number[J].Industrial Crops&Products,2004,19(1):49-57.
[12]Wen J L,Sun S L,Yuan T Q,et al.Structural elucidation of whole lignin from Eucalyptus based on preswelling and enzymatic hydrolysis[J].Green Chemistry,2015,17(3):1589-1596.
[13]Wang K,Xu F,Sun R C.Molecular characteristics of Kraft-AQ pulping lignin fractionated by sequential organic solvent extraction[J].International Journal of Molecular Sciences,2010,11:2988-3001.
[14]Vanholme R,Cesarino I,Rataj K,et al.Caffeoyl shikimate esterase(CSE)is an enzyme in the lignin biosynthetic pathway in Arabidopsis[J].Science,2013,341(6150):1103-1106.
[15]Wen J L,Sun S L,Xue B L,et al.Quantitative structural characterization of the lignins from the stem and pith of bamboo(Phyllostachys pubescens)[J].Holzforschung,2013,67(6):613-627.
[16]Kacurakova M,Capek P,Sasinkova V,et al.FT-IR study of plant cell wall model compounds:pectic polysaccharides and hemicelluloses[J].Carbohydrate Polymers,2000,43(2):195-203.
[17]Zhang X,Yu H,Huang H,et al.Evaluation of biological pretreatment with white rot fungi for the enzymatic hydrolysis of bamboo culms[J].International Biodeterioration&Biodegradation,2007,60(3):159-164.
[18]Ma X J,Cao S L,Yang X F,et al.Lignin removal and benzene-alcohol extraction effects on lignin measurements of the hydrothermal pretreated bamboo substrate[J].Bioresource Technology,2014,151C(1):244-248.
[19]Wang K,Yang H Y,Yao X,et al.Structural transformation of hemicelluloses and lignin from triploid poplar during acid-pretreatment based biorefinery process[J].Bioresource Technology,2012,116:99-106.