高能混合粒子场处理小麦并储藏的辐照效应分析
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摘要
为研究小麦经高能混合粒子场诱变后的辐照效应,本文研究了运用高能混合粒子场处理小麦,以半致矮剂量67和83 Gy对普通小麦品种川辐6号和八倍体小黑麦ZSJ11的干种子进行诱变处理,并干-20℃储藏9个月;以200 Gy~(60)Co-γ射线辐照和未辐照的干种子为对照,调查各处理下主要农艺性状。结果表明,八倍体小黑麦和普通小麦经高能混合粒子场处理后,均表现出一定的辐照生物学效应,且八倍体小黑麦的辐照敏感性低于普通小麦。67 Gy的高能混合粒子场处理对八倍体小黑麦和普通小麦的生物学效应均大于200 Gy的~(60)Co-γ射线辐照处理。83 Gy高能混合粒子场诱变已达到八倍体小黑麦和普通小麦的致死剂量,致死剂量与前人研究结果存在差异的原因可能是辐照之后的"储藏效应"影响了辐照的损伤程度。本文可为以小麦高能混合粒子场模拟空间诱变育种提供一定的参考。
In order to study the mutagenic effects of high energy mixed particles field irradiation on wheat,the dry seeds of common wheat CF6 and Octoploid Triticale ZSJ11 were irradiated by high energy mixed particles field with the HD_(50) dosages of 67 and 83 Gy and then stored under- 20 ℃ for 9 months.The seeds untreated and treated by 200 Cy γ-ray were used as control,and then their major agronomic traits were investigated.The result showed that all groups with treatment of high energy mixed particles field had mutations,and ZSJ11 had a lower sensitivity to irradiation than CF6.The biological effects of 67 Cy high energy mixed particles field irradiation on ZSJ11 and CF6 were greater than that of 200 Gy γ ray irradiation.The lethal dose of high energy mixed particles field irradiation for ZSJl 1 and CF6 was 83 Gy.The causes of the difference with pre study might be that 'storage effect' affected the irradiation damage degree after irradiation.The study could provide a certain reference for high energy particles mixed field mutation breeding in wheat.
In order to study the mutagenic effects of high energy mixed particles field irradiation on wheat,the dry seeds of common wheat CF6 and Octoploid Triticale ZSJ11 were irradiated by high energy mixed particles field with the HD_(50) dosages of 67 and 83 Gy and then stored under- 20 ℃ for 9 months.The seeds untreated and treated by 200 Cy γ-ray were used as control,and then their major agronomic traits were investigated.The result showed that all groups with treatment of high energy mixed particles field had mutations,and ZSJ11 had a lower sensitivity to irradiation than CF6.The biological effects of 67 Cy high energy mixed particles field irradiation on ZSJ11 and CF6 were greater than that of 200 Gy γ ray irradiation.The lethal dose of high energy mixed particles field irradiation for ZSJl 1 and CF6 was 83 Gy.The causes of the difference with pre study might be that 'storage effect' affected the irradiation damage degree after irradiation.The study could provide a certain reference for high energy particles mixed field mutation breeding in wheat.
引文
[1]徐冠仁.植物诱变育种学[M].北京:中国农业出版社,1996.
[2]刘录祥,郭会君,赵林姝,等.植物诱发突变技术育种研究现状与展望[J].核农学报,2009,23:1001-1007.
[3]郑家团,谢华安,王乌齐,等.水稻航天诱变育种研究进展与应用前景[J].分子植物育种,2003(1):367-371.
[4]刘录祥,王晶,赵林妹,等.作物空间诱变效应及其地面模拟研究进展[J].核农学报,2004,18:247-251.
[5]于新玲,刘录祥,乔利仙,等.高能混合粒子场辐照对花生胚小叶组织培养及植株再生的影响[J].核农学报,2012,26.
[6]赵金涛,郭新梅,裴玉贺,等.高能混合粒子场对玉米幼苗生长及抗氧化系统的影响[J].核农学报,2014,28(12):2133-2138.
[7]尚晨,张月学,唐凤兰,等.高能混合粒子场和γ射线对紫花苜蓿的诱变效应[J].草地学报,2008,16:125-128.
[8]尚晨,韩贵清,张月学,等.高能混合粒子场与~(60)Co-γ射线辐照龙牧803紫花苜蓿产量性状遗传效应研究[J].黑龙江农业科学,2011(1):99-102.
[9]郭会君,刘录祥,韩微波,等.高能混合粒子场辐照小麦的突变效应分析[J].中国农业科学,2008,41:654-660.
[10]韩微波,刘录祥,郭会君,等.高能混合粒子场辐照小麦M1代变异的SSR分析[J].核农学报,2006,20:165-168.
[11]韩微波.混合粒子场诱变小麦的生物效应与机理研究[D].西北农林科技大学,2005.
[12]刘录祥,韩微波,郭会君,等.高能混合粒子场诱变小麦的细胞学效应研究[J].核农学报,2005,19:327-331.
[13]韩冰,古佳玉,赵林姝,等.不同小麦基因型对γ射线辐照敏感性的分子解析[J].植物遗传资源学报,2014.
[14]刘录祥,赵林妹,郭会君,等.高能混合粒子场辐照冬小麦生物效应研究[J].科学技术与工程,200S(5):1642-1645.
[15]章铁.刘秀清,张金良,等.不同剂量率~(60)Co-γ射线低剂量辐射对小麦农艺性状的影响[J].中国农学通报,2008,24:220-223.
[16]W.Hoppe.Biophysies[M].2nd Ed,1983:289-300.
[17]Konzak C F,Nilan R A,Harle J R,et al.Control of factors affecting the response of plants to mutagens[J].Brookhaven Symposia in Biology,1961,14.-128-57.
[18]Conger B V,Konzak C F.Radiobiological damage;a new class identified in barley seeds stored after irradiation[J].Science,1968,162(3858).
[19]Soriano J D.The response of several rice varieties to fast neutrons[J].Radiation Botany,1971,11:341-346.
[2]刘录祥,郭会君,赵林姝,等.植物诱发突变技术育种研究现状与展望[J].核农学报,2009,23:1001-1007.
[3]郑家团,谢华安,王乌齐,等.水稻航天诱变育种研究进展与应用前景[J].分子植物育种,2003(1):367-371.
[4]刘录祥,王晶,赵林妹,等.作物空间诱变效应及其地面模拟研究进展[J].核农学报,2004,18:247-251.
[5]于新玲,刘录祥,乔利仙,等.高能混合粒子场辐照对花生胚小叶组织培养及植株再生的影响[J].核农学报,2012,26.
[6]赵金涛,郭新梅,裴玉贺,等.高能混合粒子场对玉米幼苗生长及抗氧化系统的影响[J].核农学报,2014,28(12):2133-2138.
[7]尚晨,张月学,唐凤兰,等.高能混合粒子场和γ射线对紫花苜蓿的诱变效应[J].草地学报,2008,16:125-128.
[8]尚晨,韩贵清,张月学,等.高能混合粒子场与~(60)Co-γ射线辐照龙牧803紫花苜蓿产量性状遗传效应研究[J].黑龙江农业科学,2011(1):99-102.
[9]郭会君,刘录祥,韩微波,等.高能混合粒子场辐照小麦的突变效应分析[J].中国农业科学,2008,41:654-660.
[10]韩微波,刘录祥,郭会君,等.高能混合粒子场辐照小麦M1代变异的SSR分析[J].核农学报,2006,20:165-168.
[11]韩微波.混合粒子场诱变小麦的生物效应与机理研究[D].西北农林科技大学,2005.
[12]刘录祥,韩微波,郭会君,等.高能混合粒子场诱变小麦的细胞学效应研究[J].核农学报,2005,19:327-331.
[13]韩冰,古佳玉,赵林姝,等.不同小麦基因型对γ射线辐照敏感性的分子解析[J].植物遗传资源学报,2014.
[14]刘录祥,赵林妹,郭会君,等.高能混合粒子场辐照冬小麦生物效应研究[J].科学技术与工程,200S(5):1642-1645.
[15]章铁.刘秀清,张金良,等.不同剂量率~(60)Co-γ射线低剂量辐射对小麦农艺性状的影响[J].中国农学通报,2008,24:220-223.
[16]W.Hoppe.Biophysies[M].2nd Ed,1983:289-300.
[17]Konzak C F,Nilan R A,Harle J R,et al.Control of factors affecting the response of plants to mutagens[J].Brookhaven Symposia in Biology,1961,14.-128-57.
[18]Conger B V,Konzak C F.Radiobiological damage;a new class identified in barley seeds stored after irradiation[J].Science,1968,162(3858).
[19]Soriano J D.The response of several rice varieties to fast neutrons[J].Radiation Botany,1971,11:341-346.