秋水仙碱处理莱芜小姜与莱芜大姜诱变效应比较
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摘要
以莱芜小姜和莱芜大姜为试材,采用秋水仙碱处理生姜幼芽根茎,分析了莱芜小姜与莱芜大姜后代各性状的抑制率、变异类型及突变率。结果表明:莱芜小姜VM1代性状的抑制率小于莱芜大姜;莱芜小姜的变异类型多于莱芜大姜;莱芜小姜获得的有益变异类型多、比例高且稳定性好。说明秋水仙碱处理密苗型莱芜小姜更容易获得高产稳定的变异类型。
The plumule rhizome of Laiwu big ginger and Laiwu small ginger were used as test materials which were treated with colchicine.The inhibition rate on a variety of characteristics and the types mutation of their descendants were analyzed.The results showed that the physiological damage in contemporary characteristics of Laiwu big ginger was less than that of Laiwu small ginger.The mutation types of Laiwu small ginger materials were more than that of Laiwu big ginger.The rate and type of useful mutation in Laiwu small ginger materials were more than that of Laiwu big ginger,and the useful variation in Laiwu small ginger were stable.It was easier for seedling multi ginger such as Laiwu small ginger than seedling variety ginger to obtain high yield stable variation type by colchicine.
The plumule rhizome of Laiwu big ginger and Laiwu small ginger were used as test materials which were treated with colchicine.The inhibition rate on a variety of characteristics and the types mutation of their descendants were analyzed.The results showed that the physiological damage in contemporary characteristics of Laiwu big ginger was less than that of Laiwu small ginger.The mutation types of Laiwu small ginger materials were more than that of Laiwu big ginger.The rate and type of useful mutation in Laiwu small ginger materials were more than that of Laiwu big ginger,and the useful variation in Laiwu small ginger were stable.It was easier for seedling multi ginger such as Laiwu small ginger than seedling variety ginger to obtain high yield stable variation type by colchicine.
引文
[1]刘振伟,史秀娟,李立国,等.60Co?γ射线辐照生姜诱变效应研究[J].山东农业科学,2009(6):23-26.
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[3]李庆芝,刘振伟,史秀娟,等.甲基磺酸乙酯、秋水仙碱对生姜生长的影响[J].湖北农业科学,2011,50(4):751-753.
[4]蒋云,郭元林,尹春蓉,等.小麦空间环境与60Co?γ辐射诱变效应的比较研究[J].西南农业学报,2010,23(4):1023-1027.
[5]陆瑞菊,王亦菲,孙月芳,等.花药预处理对玉米游离小孢子存活率的影响[J].实验生物学报,2005,38(3):265-271.
[6]王磊.生姜种质资源倍性分析及离体诱变与细胞悬浮培养技术研究[D].泰安:山东农业大学,2015.
[2]曾杨,高山林,王蔚,等.脱病毒生姜同源四倍体的诱导和鉴定[J].药物生物技术,2006,13(5):338-342.
[3]李庆芝,刘振伟,史秀娟,等.甲基磺酸乙酯、秋水仙碱对生姜生长的影响[J].湖北农业科学,2011,50(4):751-753.
[4]蒋云,郭元林,尹春蓉,等.小麦空间环境与60Co?γ辐射诱变效应的比较研究[J].西南农业学报,2010,23(4):1023-1027.
[5]陆瑞菊,王亦菲,孙月芳,等.花药预处理对玉米游离小孢子存活率的影响[J].实验生物学报,2005,38(3):265-271.
[6]王磊.生姜种质资源倍性分析及离体诱变与细胞悬浮培养技术研究[D].泰安:山东农业大学,2015.