空间环境诱导小麦变异及ISSR多态性研究
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摘要
为探讨空间环境诱变对小麦农艺性状和分子水平变异的影响,本试验对实践八号返回式育种卫星搭载的3份普通小麦品种(系)干种子的SP1农艺性状变异和SP4、SP5选系的ISSR分子标记多态性进行研究。结果表明,空间环境诱变后小麦SP1农艺性状与野生型差异明显,不同小麦基因型对空间环境诱变的敏感性不同。突变系与野生型的遗传相似系数介于0.8226~0.8777之间,与野生型相比,突变系在多个位点发生了DNA水平上的改变。突变系与野生型之间的Nei's遗传距离和各品种对空间环境诱变的敏感性结果不一致,其可能原因是人工选择增大了突变后代中有利变异的频率。综上,空间环境诱变引起了小麦分子水平的变异,这为我国小麦的空间育种提供了一批优异的育种材料。
To evaluate the effect of space environment mutagenesis on agronomic traits and molecular-level variation in wheat,this experiment investigated the agronomic traits in the SP1 generation and polymorphism of inter simple sequence repeat( ISSR) markers in the SP4,SP5 generations of three wheat cultivars( lines) carried by the recoverable satellite Shijian-8. The results showed that: After space environment mutagenesis,the agronomic traits of SP1 generation were significantly different from those of the wild type,and different wheat genotypes showed differential sensitivity to the space environment mutagenesis. The average genetic similarity index for the mutant lines and the wild type ranged from0. 8226 to 0. 8777. At the DNA level,multiple loci in the mutants differed from those in the wild type. The inconsistent results of the sensitivity to space environment of SP1 and Nei' s genetic distance between the mutants and the wild type may be explained by the high frequency of advantageous mutations resulting from artificial selection. Overall,this study illustrated that the space environment mutagenesis can induce mutations on molecular level in wheat,and can provide several excellent breeding materials for space breeding of wheat in China.
To evaluate the effect of space environment mutagenesis on agronomic traits and molecular-level variation in wheat,this experiment investigated the agronomic traits in the SP1 generation and polymorphism of inter simple sequence repeat( ISSR) markers in the SP4,SP5 generations of three wheat cultivars( lines) carried by the recoverable satellite Shijian-8. The results showed that: After space environment mutagenesis,the agronomic traits of SP1 generation were significantly different from those of the wild type,and different wheat genotypes showed differential sensitivity to the space environment mutagenesis. The average genetic similarity index for the mutant lines and the wild type ranged from0. 8226 to 0. 8777. At the DNA level,multiple loci in the mutants differed from those in the wild type. The inconsistent results of the sensitivity to space environment of SP1 and Nei' s genetic distance between the mutants and the wild type may be explained by the high frequency of advantageous mutations resulting from artificial selection. Overall,this study illustrated that the space environment mutagenesis can induce mutations on molecular level in wheat,and can provide several excellent breeding materials for space breeding of wheat in China.
引文
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[9]蒋云,郭元林,尹春蓉,宣朴.小麦空间环境与60Co-γ辐射诱变效应的比较研究[J].西南农业学报,2010,23(4):1023-1027
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[11]曹丽,钱鹏,张紫晋,粟永英,陈杰明,刘汉梅,杜小刚,陈洋尔,张怀渝.航天搭载小麦矮秆突变体DMR88-1矮化效应分析[J].核农学报,2015,29(11):2049-2057
[12]蒲志刚,张志勇,张志雄,向跃武,蔡平钟,张婷,李仕贵.水稻橙红色突变体的遗传分析与基因定位[J].核农学报,2012,26(3):416-419
[13]Zietkiewicz E,Rafalski A,Labuda D.Genome fingerprinting by simple sequence repeat(SSR)-snchored polymerase chain reaction amplification[J].Genomics,1994,20(2):176-183
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[15]马艳明,李斯深,范玉顶,孙海艳,李永祥,李瑞军.黄淮麦区小麦品种(系)的ISSR位点遗传多样性分析[J].植物遗传资源学报,2006,7(1):13-17
[16]杜金昆,姚颖垠,倪中福,彭惠茹,孙其信.普通小麦、斯卑尔脱小麦、密穗小麦和轮回选择后代材料ISSR分子标记遗传差异研究[J].遗传学报,2002,29(5):445-452
[17]王文恩,包满珠,张俊卫,刘国锋,宁国贵,尹少华.狗牙根辐射诱变后代变异植株的形态特征比较和ISSR分析[J].草业科学,2009,26(12):139-145
[18]沈紫微,陈本建,康俊梅,魏小兰,张蕴薇.红豆草ISSR体系优化及其在航天诱变种质鉴定中的应用[J].草业科学,2010,27(12):65-72
[19]吕兑财,黄增信,赵亚丽,郭会君,王根良,贾向红,李春华,张龙,刘录祥.实践八号育种卫星搭载植物种子的空间辐射剂量分析[J].核农学报,2008,22(1):5-8
[20]Tang Q Y,Zhang C X.Data Processing System(DPS)software with experimental design,statistical analysis and data mining developed for use in entomological research[J].Insect Science,2013,20(2):254-260
[21]周峰,易继财,张群宇,王慧,梅曼彤.水稻空间诱变后代的微卫星多态性分析[J].华南农业大学学报,2001,22(4):55-57
[22]杜文平,余桂容,宋军,徐利远.卫星搭载后玉米诱变系的SRAP分析[J].核农学报,2011,25(5):839-843
[23]谭雪,郭爽,林锦英,张华,郑岩松,谢伟平.菜薹航天诱变后代变异及其RAPD标记多态性分析[J].中国蔬菜,2011(10):54-57
[24]周桂元,洪彦彬,林坤耀,李少雄,梁炫强.花生空间诱变及SSR标记遗传多态性分析[J].中国油料作物学报,2007,29(3):238-241
[25]王心宇,陈佩度,亓增军,张政值,马正强.ISSR标记在小麦指纹图谱分析中的应用研究初探[J].农业生物技术学报,2001,9(3):261-263
[26]郭怡璠,张宏纪,孙岩,刘东军,宋波,王广金,杨淑萍,郭强,闫文义,孙光祖.春小麦空间诱变SP2的SSR标记变异分析[J].黑龙江农业科学,2010(7):9-12
[27]杨红善,常根柱,包文生,柴小琴,周学辉.紫花苜蓿航天诱变田间形态学变异研究[J].草业学报,2012,21(5):222-228
[28]王美芳,杨会民,杨攀,何宁,吴政卿,李巍,徐福新,刘加平,雷震生.冬小麦品种航天诱变后代性状分析[J].核农学报,2011,25(5):833-838
[29]骆艺,王旭杰,梅曼彤,庄楚雄,周峰,卫增泉,颉红梅,姚涓,赵均良.空间搭载水稻种子后代基因组多态性及其与空间重离子辐射关系的探讨[J].生物物理学报,2006,22(2):131-138
[30]Zaka R,Chenal C,Misset M T.Study of external low irradiation dose effects on induction of chromosome aberrations in Pisum sativum root tip meristem.[J].Mutation Research,2002,517(1):87-99
[2]刘录祥,郭会君,赵林姝,古佳玉,赵世荣.我国作物航天育种20年的基本成就与展望[J].核农学报,2007,21(6):589-592
[3]Wang J M,Wei L J,Zheng T Q,Zhao X Q,Jauhar A,Xu J L,Li Z K.Simple sequence repeat markers reveal multiple loci governing grain-size variations in a japonica,rice(Oryza sativa L.)mutant induced by cosmic radiation during space flight[J].Euphytica,2013,196(2):225-236
[4]赵一洲,倪善君,张战,李鑫,毛艇,张丽丽,刘研,刘福才.盐粳188空间诱变后代性状变异分析[J].核农学报,2016,30(9):1672-1678
[5]潘多锋,张月学,王建丽,高超,张瑞博,李道明,申忠宝,王德利,刘录祥.“实践八号”卫星搭载羊草的诱变效应及变异研究[J].核农学报,2015,29(7):1233-1238
[6]郭会君,靳文奎,赵林姝,赵世荣,赵洪兵,刘录祥.实践八号卫星飞行环境中不同因素对小麦的诱变效应[J].作物学报,2010,36(5):764-770
[7]赵洪兵,郭会君,赵林姝,古佳玉,赵世荣,李军辉,刘录祥.一个空间诱变的温度敏感型冬小麦叶绿素突变体的初步研究[J].核农学报,2010,24(6):1110-1116
[8]王伟.卫星搭载小麦农艺性状及蛋白变异分析[D].杨陵:西北农林科技大学,2011
[9]蒋云,郭元林,尹春蓉,宣朴.小麦空间环境与60Co-γ辐射诱变效应的比较研究[J].西南农业学报,2010,23(4):1023-1027
[10]吕海霞,张艳欣,王林海,危文亮,黎冬华,张晓燕,张秀荣.芝麻空间诱变后代的变异及其AFLP标记多态性分析[J].核农学报,2011,25(5):851-856
[11]曹丽,钱鹏,张紫晋,粟永英,陈杰明,刘汉梅,杜小刚,陈洋尔,张怀渝.航天搭载小麦矮秆突变体DMR88-1矮化效应分析[J].核农学报,2015,29(11):2049-2057
[12]蒲志刚,张志勇,张志雄,向跃武,蔡平钟,张婷,李仕贵.水稻橙红色突变体的遗传分析与基因定位[J].核农学报,2012,26(3):416-419
[13]Zietkiewicz E,Rafalski A,Labuda D.Genome fingerprinting by simple sequence repeat(SSR)-snchored polymerase chain reaction amplification[J].Genomics,1994,20(2):176-183
[14]Nagaoka T,Ogihara Y.Nagaoka T,Ogihara Y.Applicability of inter-simple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers[J].Theoretical Applied Genetics,1997,94(5):597-602
[15]马艳明,李斯深,范玉顶,孙海艳,李永祥,李瑞军.黄淮麦区小麦品种(系)的ISSR位点遗传多样性分析[J].植物遗传资源学报,2006,7(1):13-17
[16]杜金昆,姚颖垠,倪中福,彭惠茹,孙其信.普通小麦、斯卑尔脱小麦、密穗小麦和轮回选择后代材料ISSR分子标记遗传差异研究[J].遗传学报,2002,29(5):445-452
[17]王文恩,包满珠,张俊卫,刘国锋,宁国贵,尹少华.狗牙根辐射诱变后代变异植株的形态特征比较和ISSR分析[J].草业科学,2009,26(12):139-145
[18]沈紫微,陈本建,康俊梅,魏小兰,张蕴薇.红豆草ISSR体系优化及其在航天诱变种质鉴定中的应用[J].草业科学,2010,27(12):65-72
[19]吕兑财,黄增信,赵亚丽,郭会君,王根良,贾向红,李春华,张龙,刘录祥.实践八号育种卫星搭载植物种子的空间辐射剂量分析[J].核农学报,2008,22(1):5-8
[20]Tang Q Y,Zhang C X.Data Processing System(DPS)software with experimental design,statistical analysis and data mining developed for use in entomological research[J].Insect Science,2013,20(2):254-260
[21]周峰,易继财,张群宇,王慧,梅曼彤.水稻空间诱变后代的微卫星多态性分析[J].华南农业大学学报,2001,22(4):55-57
[22]杜文平,余桂容,宋军,徐利远.卫星搭载后玉米诱变系的SRAP分析[J].核农学报,2011,25(5):839-843
[23]谭雪,郭爽,林锦英,张华,郑岩松,谢伟平.菜薹航天诱变后代变异及其RAPD标记多态性分析[J].中国蔬菜,2011(10):54-57
[24]周桂元,洪彦彬,林坤耀,李少雄,梁炫强.花生空间诱变及SSR标记遗传多态性分析[J].中国油料作物学报,2007,29(3):238-241
[25]王心宇,陈佩度,亓增军,张政值,马正强.ISSR标记在小麦指纹图谱分析中的应用研究初探[J].农业生物技术学报,2001,9(3):261-263
[26]郭怡璠,张宏纪,孙岩,刘东军,宋波,王广金,杨淑萍,郭强,闫文义,孙光祖.春小麦空间诱变SP2的SSR标记变异分析[J].黑龙江农业科学,2010(7):9-12
[27]杨红善,常根柱,包文生,柴小琴,周学辉.紫花苜蓿航天诱变田间形态学变异研究[J].草业学报,2012,21(5):222-228
[28]王美芳,杨会民,杨攀,何宁,吴政卿,李巍,徐福新,刘加平,雷震生.冬小麦品种航天诱变后代性状分析[J].核农学报,2011,25(5):833-838
[29]骆艺,王旭杰,梅曼彤,庄楚雄,周峰,卫增泉,颉红梅,姚涓,赵均良.空间搭载水稻种子后代基因组多态性及其与空间重离子辐射关系的探讨[J].生物物理学报,2006,22(2):131-138
[30]Zaka R,Chenal C,Misset M T.Study of external low irradiation dose effects on induction of chromosome aberrations in Pisum sativum root tip meristem.[J].Mutation Research,2002,517(1):87-99