miRNA的常用检测方法研究
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摘要
microRNA(miRNA)是一类非编码RNA,在生物体内的产生过程非常复杂。miRNA在细胞增生、细胞凋亡以及肿瘤发生过程中发挥着重要调节作用。一个miRNA具有调控多个基因的功能,多个miRNA也可以调控一个基因,这种复杂的调节网络既可以通过一个miRNA来调控多个基因的表达,也可以通过几个miRNA的组合来精细调控某个基因的表达。miRNA的这种特征为解决复杂疾病提供了新的切入点,成为潜在的癌基因或抑癌基因,是肿瘤发生发展过程中有效的标志物。
MicroRNA(miRNA)is a class of non-coding RNAs that are very complex in vivo. miRNA plays an important regulatory role in cell proliferation, apoptosis, and tumorigenesis. A miRNA has the function of regulating multiple genes, and multiple miRNAs can also regulate a target gene. This complex regulatory network can regulate the expression of multiple genes through one miRNA, or can be finely regulated by a combination of several miRNAs. The expression of a gene, this feature of miRNA provides a new entry point for solving complex diseases, and becomes a potential oncogene or tumor suppressor gene, and is an effective marker in the process of tumor development.
MicroRNA(miRNA)is a class of non-coding RNAs that are very complex in vivo. miRNA plays an important regulatory role in cell proliferation, apoptosis, and tumorigenesis. A miRNA has the function of regulating multiple genes, and multiple miRNAs can also regulate a target gene. This complex regulatory network can regulate the expression of multiple genes through one miRNA, or can be finely regulated by a combination of several miRNAs. The expression of a gene, this feature of miRNA provides a new entry point for solving complex diseases, and becomes a potential oncogene or tumor suppressor gene, and is an effective marker in the process of tumor development.
引文
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[9]SUN Y,HE X,JI J,et al.A highly selective and sensitive electrochemical CS-MWCNTs/Au-NPs composite DNA biosensor for Staphylococcus aureus gene sequence detection[J].Talanta,2015(141):300-306.
[2]FANGCS,KIMKS,YU B,et al.Ultrasensitive electrochemical detection of miRNA-21 using a zinc finger protein specific to DNA-RNA hybrids[J].Analytical Chemistry,2017,89(3):2 024-2 031.
[3]ADLAKHA Y K,SAINI N.Brain microRNAs and insights into biological functions and therapeutic potential of brain enriched miRNA-128[J].Molecular Cancer,2014(13):33.
[4]LI F,PENG J,WANG J,et al.Carbon nanotube-based label-free electrochemical biosensor for sensitive detection of miRNA-24[J].Biosens Bioelectron,2014(54):158-164.
[5]DUAN S,WANG Y,WANG H,et al.A novel PCR-based approach to discover miRNA target genes[J].International Journal of Medical Sciences,2014,11(12):1 270-1 274.
[6]SCH WA R ZKOPF M,PI ERCE N A.Multiplexed miR NAnorthern blots via hybridization chain reaction[J].Nucleic Acids Research,2016,44(15):129.
[7]WANG H B,J IANG Z B,LI M.Research on the typical miRNA and target genes in squamous cell carcinoma and adenocarcinoma of esophagus cancer with DNA microarray[J].Pathology&Oncology Research,2014,20(2):245-252.
[8]PENA J T,SOHN-LEE C,ROUHANIFARD S H,et al.miRNAin situ hybridization in formaldehyde and EDC-fixed tissues[J].Nature Methods,2009,6(2):139-141.
[9]SUN Y,HE X,JI J,et al.A highly selective and sensitive electrochemical CS-MWCNTs/Au-NPs composite DNA biosensor for Staphylococcus aureus gene sequence detection[J].Talanta,2015(141):300-306.