贲门癌患者口腔菌群的分布特征
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摘要
目的描述和比较贲门癌(GCC)患者和贲门正常对照人群口腔菌群分布特征及差异,为贲门癌的早期筛查提供潜在标志物。方法选取2015年10月至2016年1月河南省林州市肿瘤医院贲门癌住院患者及2017年8月至2018年1月于同一医院经食管内镜检查贲门健康人群各33例;收集口腔唾液标本并提取DNA,通过Illumina MiniSeq测序平台对其16S rRNA V4区进行双端测序;描述并比较病例及对照人群唾液样本中微生物α、β多样性及其菌群相对丰度。结果贲门癌患者口腔唾液菌群操作分类单元(OTU)较正常对照减少(175比198,P<0.05),而Shannon指数无差异;β多样性显示两组菌群构成区分明显(P<0.001);贲门癌组唾液中占主导地位的氏菌属和韦荣球菌属相对丰度均显著高于对照组(P<0.001)。结论贲门癌患者口腔唾液菌群与贲门正常对照相比差异有统计学意义。
Objective To describe and compare the distribution and differences of oral microbiota between gastric cardia carcinoma and healthy controls, and to provide clues and insights to explore the etiology of gastric cardia carcinoma(GCC). Methods A total of 33 GCC from October 2015 to January 2016 and 33 healthy controls from August 2017 to January 2018 were enrolled in the study, from Linzhou Cancer Hospital, Henan province. Oral saliva specimens from participants were collected and microbial DNA extraction was conducted. The 16 S rRNA V4 region was sequenced by Illumina MiniSeq sequencing platform. Alpha-, beta-diversity and relative abundances were used to compare the differences between GCC and healthy control groups. Results There was a decreased Operational Taxonomic Units in GCC as compared with the healthy controls(175 vs 198, P<0.05), while the Shannon index did not show significant difference. The beta diversity was very significantly different between two groups(P<0.001). The relative abundance of Prevotella and Veillonella in the saliva of GCC was significantly higher than those of healthy controls(P<0.001). Conclusions The oral saliva microbiota of GCC is significantly different from that of healthy controls.
Objective To describe and compare the distribution and differences of oral microbiota between gastric cardia carcinoma and healthy controls, and to provide clues and insights to explore the etiology of gastric cardia carcinoma(GCC). Methods A total of 33 GCC from October 2015 to January 2016 and 33 healthy controls from August 2017 to January 2018 were enrolled in the study, from Linzhou Cancer Hospital, Henan province. Oral saliva specimens from participants were collected and microbial DNA extraction was conducted. The 16 S rRNA V4 region was sequenced by Illumina MiniSeq sequencing platform. Alpha-, beta-diversity and relative abundances were used to compare the differences between GCC and healthy control groups. Results There was a decreased Operational Taxonomic Units in GCC as compared with the healthy controls(175 vs 198, P<0.05), while the Shannon index did not show significant difference. The beta diversity was very significantly different between two groups(P<0.001). The relative abundance of Prevotella and Veillonella in the saliva of GCC was significantly higher than those of healthy controls(P<0.001). Conclusions The oral saliva microbiota of GCC is significantly different from that of healthy controls.
引文
[1] 陈万青,郑荣寿,陈志峰,等.中国4个食管癌高发区上消化道癌的流行现状 [J].中国肿瘤,2011,20:557-560.
[2] The Human Microbiome Project Consortium.structure,function and diversity of the healthy human microbiome [J].Nature,2012,486:207-214.
[3] Cunningham-Rundles S,Ahrn S,Abuav-Nussbaum R,et al.development of immunocompetence:role of micronutrients and microorganisms [J].Nutr Rev,2002,60:S68-72.
[4] Arweiler NB,Netuschil L.The oral Microbiota [J].Adv Exp Med Biol,2016,902:45-60.
[5] Michaud DS,Kelsey KT,Papathanasiou E,et al.Periodontal disease and risk of all cancers among male never smokers:an updated analysis of the Health Professionals Follow-up Study [J].Ann Oncol,2016,27:941-947.
[6] Pei Z,Bini EJ,Yang L,et al.Bacterial biota in the human distal esophagus [J].Proc Natl Acad Sci U S A,2004,101(12):4250-4255.
[7] Walters WA,Caporaso JG,Lauber CL,et al.Primer Prospector:de novo design and taxonomic analysis of barcoded polymerase chain reaction primers [J].Bioinformatics,2011,27:1159-1161.
[8] Flemer B,Warren RD,Barrett MP,et al.The oral microbiota in colorectal cancer is distinctive and predictive [J].Gut,2018,67:1454-1463.
[9] Slots J,Slots H,Bacterial and viral pathogens in saliva:disease relationship and infectious risk [J].Periodontol 2000,2011,55:48-69.
[10] Dewhirst FE,Chen T,Izard J,et al.The human oral microbiome [J].J Bacteriol,2010,192:5002-5017.
[11] Chen X,Winckler B,Lu M,et al.Oral microbiota and risk for esophageal squamous cell carcinoma in a high-rsk area of China [J].PLoS One,2015,10:e0143603.doi:10.1371/journal.pone.0143603.
[12] Liu N,Ando T,Ishiguro K,et al.Characterization of bacterial biota in the distal esophagus of Japanese patients with reflux esophagitis and Barrett's esophagus [J].BMC Infect Dis,2013,13:130.doi:10.1186/1471-2334-13-130.
[13] Hu X,Zhang Q,Hua H,et al.Changes in the salivary microbiota of oral leukoplakia and oral cancer [J].Oral Oncol,2016,56:6-8.
[14] Guerrero-Preston R,Godoy-Vitorino F,Jedlicka A,et al.16S rRNA amplicon sequencing identifies microbiota associated with oral cancer,human papilloma virus infection and surgical treatment [J].Oncotarget,2016,7:51320-51334.
[15] Costello EK,Lauber CL,Hamady M,et al.Bacterial community variation in human body habitats across space and time [J].Science,2009,326:1694-1697.
[2] The Human Microbiome Project Consortium.structure,function and diversity of the healthy human microbiome [J].Nature,2012,486:207-214.
[3] Cunningham-Rundles S,Ahrn S,Abuav-Nussbaum R,et al.development of immunocompetence:role of micronutrients and microorganisms [J].Nutr Rev,2002,60:S68-72.
[4] Arweiler NB,Netuschil L.The oral Microbiota [J].Adv Exp Med Biol,2016,902:45-60.
[5] Michaud DS,Kelsey KT,Papathanasiou E,et al.Periodontal disease and risk of all cancers among male never smokers:an updated analysis of the Health Professionals Follow-up Study [J].Ann Oncol,2016,27:941-947.
[6] Pei Z,Bini EJ,Yang L,et al.Bacterial biota in the human distal esophagus [J].Proc Natl Acad Sci U S A,2004,101(12):4250-4255.
[7] Walters WA,Caporaso JG,Lauber CL,et al.Primer Prospector:de novo design and taxonomic analysis of barcoded polymerase chain reaction primers [J].Bioinformatics,2011,27:1159-1161.
[8] Flemer B,Warren RD,Barrett MP,et al.The oral microbiota in colorectal cancer is distinctive and predictive [J].Gut,2018,67:1454-1463.
[9] Slots J,Slots H,Bacterial and viral pathogens in saliva:disease relationship and infectious risk [J].Periodontol 2000,2011,55:48-69.
[10] Dewhirst FE,Chen T,Izard J,et al.The human oral microbiome [J].J Bacteriol,2010,192:5002-5017.
[11] Chen X,Winckler B,Lu M,et al.Oral microbiota and risk for esophageal squamous cell carcinoma in a high-rsk area of China [J].PLoS One,2015,10:e0143603.doi:10.1371/journal.pone.0143603.
[12] Liu N,Ando T,Ishiguro K,et al.Characterization of bacterial biota in the distal esophagus of Japanese patients with reflux esophagitis and Barrett's esophagus [J].BMC Infect Dis,2013,13:130.doi:10.1186/1471-2334-13-130.
[13] Hu X,Zhang Q,Hua H,et al.Changes in the salivary microbiota of oral leukoplakia and oral cancer [J].Oral Oncol,2016,56:6-8.
[14] Guerrero-Preston R,Godoy-Vitorino F,Jedlicka A,et al.16S rRNA amplicon sequencing identifies microbiota associated with oral cancer,human papilloma virus infection and surgical treatment [J].Oncotarget,2016,7:51320-51334.
[15] Costello EK,Lauber CL,Hamady M,et al.Bacterial community variation in human body habitats across space and time [J].Science,2009,326:1694-1697.