ARTP诱变育种技术在尼莫克汀菌种选育中的应用
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摘要
利用新型诱变技术提高尼莫克汀的发酵水平。利用ARTP诱变技术,处理尼莫克汀菌种S1-2-50,获得高产菌种S2-1-13,摇瓶效价提高25%。应用于60吨发酵罐生产,发酵水平与初始菌种相比大幅提高了35%。根据实验结果显示,ARTP诱变技术能够有效的提高尼莫克汀的发酵水平。
To improve the fermentation level of nemadectin by new mutation technology. The ARTP mutation techology was used to treat the nemadectin S1-2-50. The high-yielding strain S2-1-13 was obtained with a 25 % increase in shake flask titer. Used in 60-tons fermentor production, the fermentation level increased by 35 % compared with the original strain. According to the experimental results, ARTP mutagenesis can effectively increase the fermentation level of nemadectin.
To improve the fermentation level of nemadectin by new mutation technology. The ARTP mutation techology was used to treat the nemadectin S1-2-50. The high-yielding strain S2-1-13 was obtained with a 25 % increase in shake flask titer. Used in 60-tons fermentor production, the fermentation level increased by 35 % compared with the original strain. According to the experimental results, ARTP mutagenesis can effectively increase the fermentation level of nemadectin.
引文
[1]郭磊,杨磊,刘远,等.ARTP诱变育种技术在菌种选育中的应用[C]//2013中国生物发酵产业年会.2013.
[2]王立言,张雪,张翀,等.常压室温等离子体(ARTP)诱变育种技术及其应用[C]//国际氨基酸产业发展高峰论坛.2013.
[3]Zhang X,Zhang X F,Li H P,et al.Atmospheric and room temperature plasma(ARTP)as a new powerful mutagenesis tool[J].Applied Microbiology&Biotechnology,2014,98(12):5387-5396.
[4]舒冬梅,王德良,宋绪磊,等.常压室温等离子体诱变(ARTP)及高通量筛选高产蛋白酶米曲霉的初探[J].中国调味品,2016,41(12):67-73.
[5]周扬,薛正莲,夏俊,等.常压室温等离子体(ARTP)诱变及高通量筛选那西肽高产菌株[J].工业微生物,2015(2):7-12.
[6]Fang M,Jin L,Zhang C,et al.Rapid Mutation of Spirulina platensis by a New Mutagenesis System of Atmospheric and Room Temperature Plasmas(ARTP)and Generation of a Mutant Library with Diverse Pheno types[J].Plos One,2013,8(10):e77046.
[7]Ma Y,Yang H,Chen X,et al.Significantly improving the yield of recombinant proteins in Bacillus subtilis,by a novel powerful mutagenesis tool(ARTP):Alkalineα-amylase as a case study[J].Protein Expr Purif,2015,114:82-88.
[8]袁军,赵犇,孙梦玉,等.常压室温等离子体(ARTP)诱变快速选育高产DHA的裂殖壶菌突变株[J].生物技术通报,2015,31(10):199-204.
[9]梁剑光,顾秋忆,秦修东,等.利用常压室温等离子体(ARTP)诱变选育高产核酸酶P1菌株[J].食品工业科技,2015,36(21):183-186.
[2]王立言,张雪,张翀,等.常压室温等离子体(ARTP)诱变育种技术及其应用[C]//国际氨基酸产业发展高峰论坛.2013.
[3]Zhang X,Zhang X F,Li H P,et al.Atmospheric and room temperature plasma(ARTP)as a new powerful mutagenesis tool[J].Applied Microbiology&Biotechnology,2014,98(12):5387-5396.
[4]舒冬梅,王德良,宋绪磊,等.常压室温等离子体诱变(ARTP)及高通量筛选高产蛋白酶米曲霉的初探[J].中国调味品,2016,41(12):67-73.
[5]周扬,薛正莲,夏俊,等.常压室温等离子体(ARTP)诱变及高通量筛选那西肽高产菌株[J].工业微生物,2015(2):7-12.
[6]Fang M,Jin L,Zhang C,et al.Rapid Mutation of Spirulina platensis by a New Mutagenesis System of Atmospheric and Room Temperature Plasmas(ARTP)and Generation of a Mutant Library with Diverse Pheno types[J].Plos One,2013,8(10):e77046.
[7]Ma Y,Yang H,Chen X,et al.Significantly improving the yield of recombinant proteins in Bacillus subtilis,by a novel powerful mutagenesis tool(ARTP):Alkalineα-amylase as a case study[J].Protein Expr Purif,2015,114:82-88.
[8]袁军,赵犇,孙梦玉,等.常压室温等离子体(ARTP)诱变快速选育高产DHA的裂殖壶菌突变株[J].生物技术通报,2015,31(10):199-204.
[9]梁剑光,顾秋忆,秦修东,等.利用常压室温等离子体(ARTP)诱变选育高产核酸酶P1菌株[J].食品工业科技,2015,36(21):183-186.