多穴位电针刺激对抗模拟失重大鼠比目鱼肌萎缩作用研究
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摘要
目的研究电针刺激对抗模拟失重大鼠比目鱼肌萎缩的作用。方法采用-30°尾吊大鼠模型模拟失重的生理效应。雄性Wistar大鼠随机分成3组,每组10只(n=10):对照组(CON)、30 d尾吊组(TS)和30 d尾吊+多穴位刺激组(TS+MA)。CON组自由活动;TS组大鼠尾吊30 d;TS+MA组大鼠尾吊30 d,外加韩氏穴位神经刺激仪双侧对称穴位隔日刺激30 min。结果 TS组大鼠比目鱼肌肌纤维束排列不规则,肌纤维横截面积变小,间质水肿并有大量炎性细胞浸润;TS+MA组与TS组变化相似,但肌细胞核数量增多,没有观察到炎性细胞浸润。与CON组比,TS和TS+MA组大鼠Ⅱ型肌纤维数量均出现显著增加(P<0.01);与TS组比,TS+MA组肌纤维数量显著下降(P<0.01)。与CON组比,TS组大鼠比目鱼肌指数显著降低(P<0.05),TS+MA组无显著差异。结论多穴位电针刺激对抗模拟失重所导致的大鼠比目鱼肌萎缩作用明显,但更好的穴位刺激处方和刺激方式有待进一步探索。
Objective To research the effects of electroacupuncture stimulation to multiple acupoint( pishu,shenshu and sanyinjiao,MA) on the soleus atrophy in simulated weightlessness rats. Methods-30° tail suspended rats were used as the model to simulate the physiological effects of weightlessness. Male Wistar rats were randomly arranged into 3 groups,n = 10 in each group: control group( CON),tail-suspension group( TS) and TS + MA group( TS + MA). TS and TS + MA were suspended for 30 d,and TS + MA were suspended and stimulated in double symmetrical MA by Hans Acupoint Nerve Stimulator per 2 d. Results The microstructure of soleus showed that the skeletal muscle fiber bundles were irregularly arranged in TS,there were smaller cross sectional area,interstitial edema and more infiltration of inflammatory cells. There were similar changes in TS + MA,but more nucleolus quantity and no infiltration of inflammatory cells. Compared with CON,the quantity of Type Ⅱ muscle fibers in the soleus fibers in TS and TS + MA increased significantly( P< 0. 01),but decreased significantly( P < 0. 01) in TS + MA compared with TS. Compared with CON,the index of soleus decreased significantly( P < 0. 01) in TS,and there were no difference in TS + MA. Conclusions The electroacupuncture stimulation is of positive effect on soleus atrophy in simulated weightlessness,but better acupoint stimulation prescription and pattern are needed to be explored.
Objective To research the effects of electroacupuncture stimulation to multiple acupoint( pishu,shenshu and sanyinjiao,MA) on the soleus atrophy in simulated weightlessness rats. Methods-30° tail suspended rats were used as the model to simulate the physiological effects of weightlessness. Male Wistar rats were randomly arranged into 3 groups,n = 10 in each group: control group( CON),tail-suspension group( TS) and TS + MA group( TS + MA). TS and TS + MA were suspended for 30 d,and TS + MA were suspended and stimulated in double symmetrical MA by Hans Acupoint Nerve Stimulator per 2 d. Results The microstructure of soleus showed that the skeletal muscle fiber bundles were irregularly arranged in TS,there were smaller cross sectional area,interstitial edema and more infiltration of inflammatory cells. There were similar changes in TS + MA,but more nucleolus quantity and no infiltration of inflammatory cells. Compared with CON,the quantity of Type Ⅱ muscle fibers in the soleus fibers in TS and TS + MA increased significantly( P< 0. 01),but decreased significantly( P < 0. 01) in TS + MA compared with TS. Compared with CON,the index of soleus decreased significantly( P < 0. 01) in TS,and there were no difference in TS + MA. Conclusions The electroacupuncture stimulation is of positive effect on soleus atrophy in simulated weightlessness,but better acupoint stimulation prescription and pattern are needed to be explored.
引文
[1]Roy RR,Baldwin KM,Edgerton VR.Response of the neuromuscular unit to spaceflight:what has been learned from the rat model[J].Exerc Sport Sci Rev,1996,24(6):399-425.
[2]Fitts RH,Riley DR,Widrick JJ.Invited Review:Microgravity and skeletal muscle[J].J Appl Physiol,2000,89(2):823-839.
[3]沈羡云,任维.失重生理学基础与进展[M].北京:国防工业出版社,2007:186-195.Shen XY,Ren W.Weightless physiological base and progress[M].Beijing:National Defense Industry Press,2007:186-195.
[4]陈杰,马进,丁兆平,等.一种模拟失重影响的大鼠尾吊模型[J].空间科学学报,1993,13(2):159-162.Chen J,Ma J,Ding ZP,et al.A modified tail-suspension model for simulating long-term weightlessness[J].Chinese Journal of Space Science,1993,13(2):159-162.
[5]华兴邦,李辞蓉,周皓良,等.大鼠穴位图谱的研制[J].实验动物与动物实验,1999,1(1):1-5.Hua XB,Li CR,Zhou HL,et al.The development of the atlas of the rat acupoints[J].Experimental Animal and Animal Experiment,1999,1(1):1-5.
[6]Fitts RH,Riley DR,Widrick JJ.Functional and structural adaptations of skeletal muscle to microgravity[J].J Exp Biol,2001,204(18):3201-3208.
[7]Widrick JJ,Knuth ST,Norenberg KM,et al.Effects of 17days spaceflight on contractile properties of human soleus muscle fibers[J].J Physiol,1999,516:915-930.
[8]Mukhina AM,Altaeva EG,Nemirovskaya TL,et al.The role ofⅠ-type calcium channels in the accumulation of Ca2+in soleus muscle fibers in the rat and changes in the ratio of myosin and serca isoforms in conditions of gravitational unloading[J].Neurosci Behav Physiol,2008,38(2):181-188.
[9]Moriggi M,Cassano P,Vasso M,et al.A DIGE approach for the assessment of rat soleus muscle changes during unloading:effect of acetyl-L-carnitine supplementation[J].Proteomics,2008,8(17):3588-3604.
[10]Yu ZB,Gao F,Feng HZ,et al.Differential regulation of myofilament protein isoforms underlying the contractility changes in skeletal muscle unloading[J].Am J Physiol Cell Physiol,2007,292(3):C1192-1203.
[11]李莉,刘红菊,杨明浩,等.去负荷小鼠比目鱼肌收缩特性和纤维类型转化[J].中国应用生理学杂志,2012,28(2):97-101.Li L,Liu HJ,Yang MH,et al.The relationship between contractile characteristics and fiber type conversion in hindlimb unloading mice soleus[J].Chinese Journal of Applied Physiology,2012,28(2):97-101.
[12]上海中医学院.针灸学[M].北京:人民卫生出版社,1974:305.Shanghai TCM College.Acupuncturology[M].Beijing:People's Medical Publishing House,1974:305-305.
[13]王广志,张倩,黄志光,等.单片机控制的韩氏穴位神经刺激仪[J].中国医疗器械杂志,1999,23(5):277-290Wang GZ,Zhang Q,Huang ZG,et al.Single-chip computer controlled acupoint nerve stimulator[J].Chinese Journal of Medical Instrumentation,1999,23(5):277-290.
[14]辛小玲.骨痿的辨证论治研究[D].北京:中国中医科学院,2012.Xin XL.Researches on diagnosis and treatment of atrophic debility of bones[D].Beijing:China Academy of Chinese Medical Sciences,2012:16.
[15]Fitts RH,Trappe SW,Costill DL,et al.Prolonged spacef1ight-induced alterations in the structure and function of human skeletal muscle fibres[J].J Physiol,2010,588(18):3567-3592.
[2]Fitts RH,Riley DR,Widrick JJ.Invited Review:Microgravity and skeletal muscle[J].J Appl Physiol,2000,89(2):823-839.
[3]沈羡云,任维.失重生理学基础与进展[M].北京:国防工业出版社,2007:186-195.Shen XY,Ren W.Weightless physiological base and progress[M].Beijing:National Defense Industry Press,2007:186-195.
[4]陈杰,马进,丁兆平,等.一种模拟失重影响的大鼠尾吊模型[J].空间科学学报,1993,13(2):159-162.Chen J,Ma J,Ding ZP,et al.A modified tail-suspension model for simulating long-term weightlessness[J].Chinese Journal of Space Science,1993,13(2):159-162.
[5]华兴邦,李辞蓉,周皓良,等.大鼠穴位图谱的研制[J].实验动物与动物实验,1999,1(1):1-5.Hua XB,Li CR,Zhou HL,et al.The development of the atlas of the rat acupoints[J].Experimental Animal and Animal Experiment,1999,1(1):1-5.
[6]Fitts RH,Riley DR,Widrick JJ.Functional and structural adaptations of skeletal muscle to microgravity[J].J Exp Biol,2001,204(18):3201-3208.
[7]Widrick JJ,Knuth ST,Norenberg KM,et al.Effects of 17days spaceflight on contractile properties of human soleus muscle fibers[J].J Physiol,1999,516:915-930.
[8]Mukhina AM,Altaeva EG,Nemirovskaya TL,et al.The role ofⅠ-type calcium channels in the accumulation of Ca2+in soleus muscle fibers in the rat and changes in the ratio of myosin and serca isoforms in conditions of gravitational unloading[J].Neurosci Behav Physiol,2008,38(2):181-188.
[9]Moriggi M,Cassano P,Vasso M,et al.A DIGE approach for the assessment of rat soleus muscle changes during unloading:effect of acetyl-L-carnitine supplementation[J].Proteomics,2008,8(17):3588-3604.
[10]Yu ZB,Gao F,Feng HZ,et al.Differential regulation of myofilament protein isoforms underlying the contractility changes in skeletal muscle unloading[J].Am J Physiol Cell Physiol,2007,292(3):C1192-1203.
[11]李莉,刘红菊,杨明浩,等.去负荷小鼠比目鱼肌收缩特性和纤维类型转化[J].中国应用生理学杂志,2012,28(2):97-101.Li L,Liu HJ,Yang MH,et al.The relationship between contractile characteristics and fiber type conversion in hindlimb unloading mice soleus[J].Chinese Journal of Applied Physiology,2012,28(2):97-101.
[12]上海中医学院.针灸学[M].北京:人民卫生出版社,1974:305.Shanghai TCM College.Acupuncturology[M].Beijing:People's Medical Publishing House,1974:305-305.
[13]王广志,张倩,黄志光,等.单片机控制的韩氏穴位神经刺激仪[J].中国医疗器械杂志,1999,23(5):277-290Wang GZ,Zhang Q,Huang ZG,et al.Single-chip computer controlled acupoint nerve stimulator[J].Chinese Journal of Medical Instrumentation,1999,23(5):277-290.
[14]辛小玲.骨痿的辨证论治研究[D].北京:中国中医科学院,2012.Xin XL.Researches on diagnosis and treatment of atrophic debility of bones[D].Beijing:China Academy of Chinese Medical Sciences,2012:16.
[15]Fitts RH,Trappe SW,Costill DL,et al.Prolonged spacef1ight-induced alterations in the structure and function of human skeletal muscle fibres[J].J Physiol,2010,588(18):3567-3592.