强骨抗萎方增强尾吊大鼠承重骨骨矿密度（英文）
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摘要
目的研究中药复方-强骨抗萎方(QGKWP)对尾吊大鼠骨代谢的影响。方法 21只雄性SD大鼠随机分为对照组(Con),尾吊组(TS)和强骨抗萎方处理组(QGKWP),分别填喂2.5 mL生理盐水或30mg/g中药复方,早晚各1次。每周利用双能X射线吸收测定法检测其骨密度。实验28天后,取血清测定碱性磷酸酶(ALP)、骨碱性磷酸酶(BALP)、骨γ碳氧谷氨酸蛋白(BGLAP)和脱氧吡啶(DPD)。同时检测承重骨力学性能、骨干重和骨灰重。免疫组化检测骨I型胶原(typeⅠcollagen),骨钙素(osteocalcin)和骨桥蛋白(osteopontin)含量。结果与尾吊组相比,强骨抗萎方组股骨、胫骨和腰椎骨密度显著升高,血清ALP和BGLAP表达增加。QGKWP还能明显升高尾吊大鼠股骨I型胶原和骨桥蛋白的表达。结论强骨抗萎方可部分逆转由尾吊引起的失重效应,在对抗骨丢失方面有潜在应用价值。
Objective To investigate the effects of traditional Chinese compound prescription-Qiang Gu Kang Wei Prescription( QGKWP) on bone metabolism of tail suspended rats. Methods Twenty one male SD rats were divided into control group( Con),the tail suspension group( TS) and QGKWP treatment group( QGKWP) randomly. Rats were treated with 2. 5 mL saline or 30 mg / g QGKWP by gavage every morning and afternoon,respectively. The bone mineral density( BMD) of rats was detected with dual energy X-ray absorptiometry every week. After 28 d of test,blood serum was collected for determination of alkaline phosphatase( ALP),bone alkaline phosphatase( BALP),bone gamma-carboxyglutamic acid-containing protein( BGLAP)and deoxypyridinoline( DPD). The mechanical property of weight-bearing bone,bone dry weight and bone ash weight were observed. Collagen I,osteocalcin and osteopontin were detected with immuno-histo-chemistry( IHC) staining. Results Compared with TS,QGKWP significantly increased the BMD of femur,tibia and lumbar vertebrae. QGKWP also promoted serum ALP and BGLAP level. Quantitative IHC analysis showed that QGKWP increased the expression of collagen I and osteopontin in femur of TS rats. Conclusion QGKWP partially reverses the effects induced by TS and may be a potential countermeasure for bone loss.
Objective To investigate the effects of traditional Chinese compound prescription-Qiang Gu Kang Wei Prescription( QGKWP) on bone metabolism of tail suspended rats. Methods Twenty one male SD rats were divided into control group( Con),the tail suspension group( TS) and QGKWP treatment group( QGKWP) randomly. Rats were treated with 2. 5 mL saline or 30 mg / g QGKWP by gavage every morning and afternoon,respectively. The bone mineral density( BMD) of rats was detected with dual energy X-ray absorptiometry every week. After 28 d of test,blood serum was collected for determination of alkaline phosphatase( ALP),bone alkaline phosphatase( BALP),bone gamma-carboxyglutamic acid-containing protein( BGLAP)and deoxypyridinoline( DPD). The mechanical property of weight-bearing bone,bone dry weight and bone ash weight were observed. Collagen I,osteocalcin and osteopontin were detected with immuno-histo-chemistry( IHC) staining. Results Compared with TS,QGKWP significantly increased the BMD of femur,tibia and lumbar vertebrae. QGKWP also promoted serum ALP and BGLAP level. Quantitative IHC analysis showed that QGKWP increased the expression of collagen I and osteopontin in femur of TS rats. Conclusion QGKWP partially reverses the effects induced by TS and may be a potential countermeasure for bone loss.
引文
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[28]Landis WJ,Hodgens KJ,Block D,et al.Spaceflight effects on cultured embryonic chick bone cells[J].Journal of Bone and Mineral Research,2000,15(6):1099-1112.
[29]Kumei Y,Morita S,Katano H,et al.Microgravity signal ensnarls cell adhesion,cytoskelleton,and matrix proteins of rat osteoblasts-Osteopontin,CD44,osteonectin,and alpha-tubulin[C]//Diederich M.Signal Transduction Pathways,Pt A:Apoptotic and Extracellular Signaling.Oxford:Blackwell Publishing,2006:311-317.
[2]Yang P,Jia B,Ding C,et al.Whole-body vibration effects on bone before and after hind-limb unloading in rats[J].Aviation Space and Environmental Medicine,2009,80(2):88-93.
[3]Wong RWK,Rabie ABM.Traditional Chinese medicines and bone formation-A review[J].Journal of Oral and Maxillofacial Surgery,2006,64(5):828-837.
[4]Shi HZ.Application of traditional Chinese medicine in space medical research[J].Space Medicine&Medical Engineering,2001,14(1):75-78.
[5]Zhu J.Influences of traditional Chinese medicine intervention on the bone growth and metabolism of rats with simulated weightlessness[J].Asian Pacific Journal of Tropical Medicine,2013,6(3):224-227.
[6]Fan QC,Li YZ,Shi HZ,et al.Effects of Chinese herb-compounds on bone metabolism under head-down bed rest[J].Space Medicine&Medical Engineering,2004,17(6):402-405.
[7]Li YZ,Shi HZ,Fan QC,et al.Effects of Qiang Gu Kang Wei compound prescription on biochemical indices of bone and related organs in rats under simulated weightlessness[J].Space Medicine&Medical Engineering,2003,16(2):103-106.
[8]Turner CH,Burr DB.Basic biomechanical measurements of bone-A tutorial[J].Bone,1993,14(4):595-608.
[9]Yu Q,Chen Y,Han LJ,et al.Quantitative analysis of histological images on rabbit's athrosclerotic lesions by using Image J software[J].Chinese Journal of Histochemistry and Cytochemistry,2015,24(4):297-301.
[10]Gao SG,Cheng L,Li KH,et al.Effect of epimedium pubescen flavonoid on bone mineral status and bone turnover in male rats chronically exposed to cigarette smoke[J].BMC Musculoskeletal Disorders,2012,13:105.
[11]Song SH,Yu Q,Wang ZZ,et al.Research progress of Drynariae Rhizoma in the prevention and treatment of osteoporosis[J].Chemistry of Life,2015,35(1):73-80.
[12]Li Y,Kang Q,Rong C,et al.Effect of drynaria total flavonoids on the proliferation,differentiation,mineralization,and apoptosis of MLO-Y4 cells[J].Chinese Journal of Osteoporosis,2015,21(5):592-598.
[13]Liu P,Wang DX,Chen GY,et al.Effect of compounds extracted from spatholobus suberectus dunn on the proliferation of hematopoietic progenitor cell[J].Chinese Pharmacological Bulletin,2007,23(6):741-745.
[14]Li YZ,Shi HZ,Fan QC,et al.Effects of Qiang Gu Kang Wei compound on osteoblasts cultured in vitro in simulated weightlessness condition[J].Chinese Journal of Aerospace Medicine,2003,14(4):196-199.
[15]Lecoq B,Potrel-Burgot C,Granier P,et al.Comparison of bone loss induced in female rats by hindlimb unloading,ovariectomy,or both[J].Joint Bone Spine,2006,73(2):189-195.
[16]Riggs BL,Hodgson SF,Ofallon WM,et al.effect of fluoride treatment on the fracture rate in postmenopausal women with osteoporosis[J].New England Journal of Medicine,1990,322(12):802-809.
[17]Chappard D,Petitjean M,Alexandre C,et al.Cortical osteoclasts are less sensitive to etidronate than trabecular osteoclasts[J].Journal of Bone and Mineral Research,1991,6(7):673-680.
[18]Chen HY,Emura S,Isono H,et al.Effects of traditional Chinese medicine on bone loss in SAMP6:A murine model for senile osteoporosis[J].Biological&Pharmaceutical Bulletin,2005,28(5):865-869.
[19]Grindeland RE,Ballard RW,Connolly JP,et al.COSMOS2044 mission.Overview[J].Journal of Applied Physiology,1992,73(2):1S-3S.
[20]Lv HC,Zhang LC,Yang F,et al.Comparison of microstructural and mechanical properties of trabeculae in femoral head from osteoporosis patients with and without cartilage lesions:a case-control study[J].BMC Musculoskeletal Disorders,2015,16:72.
[21]Wang ZQ,Li JL,Sun YL,et al.Chinese herbal medicine for osteoporosis:a systematic review of randomized controlled trails[J].Evidence-Based Complementary and Alternative Medicine,2013:e356260
[22]Brown JP,Malaval L,Chapuy MC,et al.Serum bone Gla-protein:a specific marker for bone formation in postmenopausal osteoporosis[J].Lancet,1984,1(8386):1091-1093.
[23]Houillier P,Froissart M,Maruani G,et al.What serum calcium can tell us and what it can't[J].Nephrology Dialysis Transplantation,2006,21(1):29-32.
[24]Goransson LG,Skadberg O,Bergrem H.Albumin-corrected or ionized calcium in renal failure?What to measure?[J].Nephrology Dialysis Transplantation,2005,20(10):2126-2129.
[25]Seitzer U,Bodo M,Muller PK,et al.Microgravity and hypergravity effects on collagen biosynthesis of human dermal fibroblasts[J].Cell and Tissue Research,1995,282(3):513-517.
[26]Carmeliet G,Nys G,Stockmans I,et al.Gene expression related to the differentiation of osteoblastic cells is altered by microgravity[J].Bone,1998,22(5):139S-143S.
[27]Bucaro MA,Zahm AM,Risbud MV,et al.The effect of simulated Microgravity on Osteoblasts is independent of the induction of apoptosis[J].Journal of Cellular Biochemistry,2007,102(2):483-495.
[28]Landis WJ,Hodgens KJ,Block D,et al.Spaceflight effects on cultured embryonic chick bone cells[J].Journal of Bone and Mineral Research,2000,15(6):1099-1112.
[29]Kumei Y,Morita S,Katano H,et al.Microgravity signal ensnarls cell adhesion,cytoskelleton,and matrix proteins of rat osteoblasts-Osteopontin,CD44,osteonectin,and alpha-tubulin[C]//Diederich M.Signal Transduction Pathways,Pt A:Apoptotic and Extracellular Signaling.Oxford:Blackwell Publishing,2006:311-317.