支原体肺炎儿童与健康儿童血清拉曼光谱检测的差异性研究
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摘要
目的探讨血清拉曼光谱检测技术在支原体肺炎儿童与健康儿童的差异性。方法收集商洛市中心医院2017年4～12月收治的支原体肺炎患儿血清100例作为实验组,选取同期健康儿童的血清100例作为对照组,使用全自动显微拉曼系统检测两组血清的拉曼光谱信号,对差异性进行分析。结果与对照组相比,实验组血清在位移1 653.34 cm~(-1)处拉曼光谱峰发生了减弱,差异有统计学意义(t=2.137,P<0.05),在位移1 152.93和1 515.33 cm~(-1)处拉曼光谱峰得到了增强,差异有统计学意义(t=2.027和2.028,均P<0.05)。结论初步研究表明支原体肺炎儿童与健康儿童血清拉曼光谱信号存在差异。
Objective To investigate the difference of serum Raman spectroscopy in children with Mycoplasma pneumoniae pneumonia(MPP)and healthy children.Methods The serum in 100 cases of children with MPP from April to December 2017 in the Shangluo Central Hospital were selected as experimental group,and 100 cases of healthy children's serum were selected as the control group.The Raman spectral signals from two groups of serum were detected using a fully automated micro Raman system,and the data was analyzed.Results Compared with control group,the experimental group children showed lower levels of the intensities at 1 653.34 cm~(-1)(t=2.137,P<0.05),respectively,but higher levels of the intensities at 1 152.93 and 1 515.33 cm~(-1)(t=2.027,2.028,all P<0.05).Conclusion This preliminary studies demonstrated that there was a difference in serum Raman spectral signals between children with MPP and healthy children.
Objective To investigate the difference of serum Raman spectroscopy in children with Mycoplasma pneumoniae pneumonia(MPP)and healthy children.Methods The serum in 100 cases of children with MPP from April to December 2017 in the Shangluo Central Hospital were selected as experimental group,and 100 cases of healthy children's serum were selected as the control group.The Raman spectral signals from two groups of serum were detected using a fully automated micro Raman system,and the data was analyzed.Results Compared with control group,the experimental group children showed lower levels of the intensities at 1 653.34 cm~(-1)(t=2.137,P<0.05),respectively,but higher levels of the intensities at 1 152.93 and 1 515.33 cm~(-1)(t=2.027,2.028,all P<0.05).Conclusion This preliminary studies demonstrated that there was a difference in serum Raman spectral signals between children with MPP and healthy children.
引文
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[2] 周克亮.阿奇霉素序贯疗法治疗小儿支原体肺炎疗效分析[J].中国实用医药,2015,10 (36):132-133.ZHOU Keliang.Clinical analysis of azithromycin sequential herapy in the treatment of children with Mycoplasma pneumoniae pneumonia[J].China Practical Medical,2015,10(36):132-133.
[3] 江锡娟.小儿肺炎支原体肺炎并发症防治进展[J].临床医药文献杂志,2018,5(12):191-193.JIANG Xijuan.Progress in prevention and treatment of complications of Mycoplasma pneumonia pneumonia in children[J].Journal of Clinical Medical Literature,2018,5(12):191-193.
[4] 王良玉,辛德莉.肺炎支原体感染实验室诊断的研究进展[J].传染病信息,2017,30(1):51-55.WANG Liangyu,XIN Deli.Research progress on laboratory diagnostic techniques for Mycoplasma pneumoniae infection[J].Infectious Disease Information,2017,30(1):51-55.
[5] 田国辉,陈亚杰,冯青茂.拉曼光谱的发展及应用[J].化学工程师,2008,22(1):34-36.TIAN Guohui,CHEN Yajie,FENG Qingmao.Development and application of Raman technology[J].Chemical Engineer,2008,22(1):34-36.
[6] XIAO Rui,ZHANG Xuhui,RONG Zhen,et al.Non-invasive detection of hepatocellular carcinoma serum metabolic profile through surface-enhanced Raman spectroscopy[J].Nanomedicine,2016,12(8):2475-2484.
[7] LI Xiaozhou,YANG Tianyue,LI Siqi,et al.Noninvasive liver diseases detection based on serum surface enhanced Raman spectroscopy and statistical analysis[J].Opt Express,2015,23(14):19361-18372.
[8] WANG Jing,FENG Shangyuan,LIN Juqiang,et al.Serum albumin and globulin analysis for hepatocellular carcinoma detection avoiding false-negative results from alpha-fetoprotein test negative subjects[J].Appl Phys Lett,2013,103(20):204106.
[9] 殷勇,陆权,闫晓莉,等.肺炎支原体感染的流行病学[J].中华儿科杂志,2016,54(2):91-93.YIN Yong,LU Quan,YAN Xiaoli,et al.The epidemiology of Mycoplasma pneumoniae infection[J].Chin J Pediatr,2016,54(2):91-93.
[10] 于国慧,吕志勇,宋文琪.儿童疑似肺炎支原体感染者血清、支气管灌洗液、胸腔积液、脑脊液MP-Ab-IgM检测的结果分析[J].现代检验医学杂志,Y Guohui,L Zhiyong,SONG Wenqi.Analysis of the results of MP-Ab-IgM detection of serum,bronchial lavage fluid,pleural effusion and cerebrospinal fluid in children suspected of Mycoplasma pneumoniae infection[J].Journal of Modern Laboratory Medicine,2018,33(2),93-96.
[11] 李正秋,刘兰香,刘云花.两种不同方法检测儿童肺炎支原体感染的对比分析[J].中国中西医结合儿科学,2016,8(4):423-426.LI Zhengqiu,LIU Lanxiang,LIU Yunhua.A compa-rative analysis of two different methods for detection of Mycoplasma pneumoniae infection in children[J].Chinese Pediatrics of Integrated Traditional and Western Medicine,2016,8(4):423-426.
[12] 宋小芳.儿童肺炎支原体感染的三种检测方法的临床应用分析[J].临床医药实践,2018,27(10):767-770.SONG Xiaofang.Clinical application analysis of thre-e methods to diagnose MP infection in children[J].Proceeding of Clinical Medicine,2018,27(10):767-770.