膝骨关节炎中医证候尿液生物标志物研究
|
摘要
目的:研究膝骨关节炎(KOA)中医证候的尿液生物标志物特征,为KOA中医证候提供新内容。方法:2012年5月—2013年12月收集KOA病例120例,男57例,女63例,分为肝肾不足、筋脉瘀滞证,脾肾两虚、湿注骨节证,肝肾亏虚、痰瘀交阻证;健康对照组55例,男27例,女28例,所有受试者来自辽宁中医药大学。采用ELISA法用酶标仪测定尿液I型和II型胶原抗原表位C1,2C、II型胶原抗原表位C2C、Ⅱ型胶原羧基前肽(CTX-Ⅱ),整理分析KOA中医证候尿液生物标志物,研究KOA中医证候尿液生物标志物特征。结果:本研究结果表明KOA三种证型尿液C1,2C、CTX-Ⅱ明显增高(P<0.05),肝肾亏虚、痰瘀交阻证C1,2C较肝肾不足、筋脉瘀滞证和脾肾两虚、湿注骨节证显著增加(P<0.05),但肝肾亏虚、痰瘀交阻证CTX-Ⅱ较肝肾不足、筋脉瘀滞证和脾肾两虚、湿注骨节证减少(P<0.05);肝肾不足、筋脉瘀滞证C2C和CTX-Ⅱ之间及肝肾亏虚、痰瘀交阻证C1,2C和C2C之间显示正相关(P<0.05)。结论:膝骨关节炎尿液C1,2C和CTX-Ⅱ具有KOA中医证候各证型生物标志物特征。
Objective:The objective is to study the urine biomarker characteristics of the traditional Chinese medicine syndrome of knee osteoarthritis(KOA). This method provides new content for traditional Chinese medicine symptomatology of KOA. Methods:We collect the cases of KOA in 120 cases(57 male,63 female)last year(2012.05-2013.12). These cases contains the following traditional Chinese medicine syndrome types:liver And kidney deficiencies and meridian stasis syndrome,asthenia of both the spleen and kidney and wet condyle syndrome,liver and kidney deficiency,phlegm retention syndrome. A total of 55 cases of healthy control group(27 male,28 female from the Liaoning University of Traditional Chinese Medicine). These indicators C1(Type I collagen epitope),2C(type II collagen epitope),C2C(Type II collagen epitope),CTX-II(type II procollagen peptide)were detected with ELISA Kit. Then we sorted out biological markers and clinical symptoms of KOA syndrome to explore the urine biomarker characteristics of the traditional Chinese medicine syndrome of KOA. Results:The results of this study show that C1,2C,CTX-Ⅱincreased in the three kinds of syndromes of urine. C1 and 2C icreased significantly in the liver and kidney deficiencies and meridian stasis syndrome of the three kinds of syndromes of urine(P<0.05),but CTX-Ⅱ was lower than the other(P<0.05).Between the liver and kidney deficiency,stasis of the channels of C2 C and CTX-Ⅱ showed positive correlation in the liver and kidney deficiencies and meridian stasis syndrome(P<0.05). C1,2C and C2 C in liver and kidney deficiency with phlegm retention syndrome also appeared similar case(P<0.05). Conclusion:C1,2C和CTX-Ⅱ in the urine of the KOA have the traditional Chinese medicine syndromes characteristics of biomarkers.
Objective:The objective is to study the urine biomarker characteristics of the traditional Chinese medicine syndrome of knee osteoarthritis(KOA). This method provides new content for traditional Chinese medicine symptomatology of KOA. Methods:We collect the cases of KOA in 120 cases(57 male,63 female)last year(2012.05-2013.12). These cases contains the following traditional Chinese medicine syndrome types:liver And kidney deficiencies and meridian stasis syndrome,asthenia of both the spleen and kidney and wet condyle syndrome,liver and kidney deficiency,phlegm retention syndrome. A total of 55 cases of healthy control group(27 male,28 female from the Liaoning University of Traditional Chinese Medicine). These indicators C1(Type I collagen epitope),2C(type II collagen epitope),C2C(Type II collagen epitope),CTX-II(type II procollagen peptide)were detected with ELISA Kit. Then we sorted out biological markers and clinical symptoms of KOA syndrome to explore the urine biomarker characteristics of the traditional Chinese medicine syndrome of KOA. Results:The results of this study show that C1,2C,CTX-Ⅱincreased in the three kinds of syndromes of urine. C1 and 2C icreased significantly in the liver and kidney deficiencies and meridian stasis syndrome of the three kinds of syndromes of urine(P<0.05),but CTX-Ⅱ was lower than the other(P<0.05).Between the liver and kidney deficiency,stasis of the channels of C2 C and CTX-Ⅱ showed positive correlation in the liver and kidney deficiencies and meridian stasis syndrome(P<0.05). C1,2C and C2 C in liver and kidney deficiency with phlegm retention syndrome also appeared similar case(P<0.05). Conclusion:C1,2C和CTX-Ⅱ in the urine of the KOA have the traditional Chinese medicine syndromes characteristics of biomarkers.
引文
[1]李宁华.中老年人群骨关节炎的流行病学特征[J].中国临床康,2005,9(38):133-135.
[2]吕厚山,孙铁铮,刘忠厚.骨关节炎的诊治与研究进展[J].中国骨质疏松杂志,2004,10(1):7-22,60.
[3]王定,史晓林,李文庆,等.膝骨性关节炎中医药治疗的研究进展[J].中国中医骨伤科杂志,2008,16(4):65-67.
[4]De Ceuninck F,Sabatini M,Pastoureau P.Recent progress toward biomarker identification in osteoarthritis[J].Drug Discov Today,2011,16(9/10):443-449.
[5]Garnero P,Delmas PD.Biomarkers in osteoarthritis[J].Curr Opin Rheumatol,2003,15:641-646.
[6]Jansen NW,Roosendaal G,Lundin B,et al.The combination of the biomarkers urinary C-terminal telopeptide of type II collagen,serum cartilage oligomeric matrix protein,and serumchondroitin sulfate 846 reflects cartilage damage in hemophilic arthropathy[J].Arthritis Rheum,2009,60(1):290-298.
[2]吕厚山,孙铁铮,刘忠厚.骨关节炎的诊治与研究进展[J].中国骨质疏松杂志,2004,10(1):7-22,60.
[3]王定,史晓林,李文庆,等.膝骨性关节炎中医药治疗的研究进展[J].中国中医骨伤科杂志,2008,16(4):65-67.
[4]De Ceuninck F,Sabatini M,Pastoureau P.Recent progress toward biomarker identification in osteoarthritis[J].Drug Discov Today,2011,16(9/10):443-449.
[5]Garnero P,Delmas PD.Biomarkers in osteoarthritis[J].Curr Opin Rheumatol,2003,15:641-646.
[6]Jansen NW,Roosendaal G,Lundin B,et al.The combination of the biomarkers urinary C-terminal telopeptide of type II collagen,serum cartilage oligomeric matrix protein,and serumchondroitin sulfate 846 reflects cartilage damage in hemophilic arthropathy[J].Arthritis Rheum,2009,60(1):290-298.