冬小麦东农冬麦1号在不同温度下的代谢组学差异分析
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摘要
为明确东农冬麦1号耐寒越冬的生理机制,对不同温度条件下的东农冬麦1号幼苗分蘖节进行GC-TOF/MS代谢组学检测,并采用SIMCA软件对获得的代谢组学数据进行多元变量模式识别分析。结果表明,对温度变化反应明显的代谢产物有54个,对其进行KEGG分析发现,变化显著的代谢通路为:精氨酸和脯氨酸代谢;甘氨酸、丝氨酸和苏氨酸代谢;丙氨酸、天冬氨酸和谷氨酸盐代谢;丙酮酸代谢;糖酵解和糖异生以及肌醇磷酸盐代谢。分析不同温度下以上代谢通路中的差异化合物发现,室内培养组中,分蘖节中的γ-氨基丁酸、蔗糖-6-磷酸以及尿素等含量显著提高,使植株保持了较高的氮素含量,揭示了较高温度下(室内)小麦出现徒长的原因。研究结果可为进一步研究冬小麦越冬的生理特性提供新的研究思路。
To clarify the cold-resistant and cold hardening mechanism of Dongnongdongmai 1,the tillering nodes were collected from different temperature conditions for the study.The modern metabolomics research method(GC-TOF/MS)was employed to test the tillering nodes.Then the SIMCA software was used to analyze the data.The results showed that,54 metabolites which presented significant changes were detected.Significant changes of metabolic pathways were obtained through KEGG analysis,such as arginine and proline metabolism;glycine,serine and threonine metabolism;alanine,aspartate and glutamate metabolism;pyruvate metabolism;glycolysis or gluconeogenesis and inositol phosphate metabolism.As mentioned above,theγ-aminobutyric acid,sucrose-6-phosphate and urea were found to present the most significant changes in indoor culture group,which maintained the content of nitrogen at a high dynamic balance value.Therefore,these results could explain the phenomenon that the wheat at high temperature(room temperature)showed an excessive growth.In conclusion,the results of this study provided a new scientific research idea for further study of the physiological characteristics of winter wheat.
To clarify the cold-resistant and cold hardening mechanism of Dongnongdongmai 1,the tillering nodes were collected from different temperature conditions for the study.The modern metabolomics research method(GC-TOF/MS)was employed to test the tillering nodes.Then the SIMCA software was used to analyze the data.The results showed that,54 metabolites which presented significant changes were detected.Significant changes of metabolic pathways were obtained through KEGG analysis,such as arginine and proline metabolism;glycine,serine and threonine metabolism;alanine,aspartate and glutamate metabolism;pyruvate metabolism;glycolysis or gluconeogenesis and inositol phosphate metabolism.As mentioned above,theγ-aminobutyric acid,sucrose-6-phosphate and urea were found to present the most significant changes in indoor culture group,which maintained the content of nitrogen at a high dynamic balance value.Therefore,these results could explain the phenomenon that the wheat at high temperature(room temperature)showed an excessive growth.In conclusion,the results of this study provided a new scientific research idea for further study of the physiological characteristics of winter wheat.
引文
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[24]LANCINE M,ROBERTS M R.Regulation of Arabidopsis thaliana 14-3-3gene expression by g-aminobutyric acid[J].Plant Cell Environment,2006,29(7):1434.
[25]SU G X,YU B J,ZHANG W H,et al.Higher accumulation of g-aminobutyric acid induced by salt stress through stimulating the activity of diamine oxidases in Glycine max(L.)Merr.roots[J].Plant Physiology and Biochemistry,2007,45(8):560.
[26]DITTAMI S M,GRAVOT A,RENAULT D,et al.Integrative analysis of metabolite and transcript abundance during the short-term response to saline and oxidative stress in the brown algaEctocarpus siliculosus[J].Plant Cell Environment,2011,34(4):629.
[27]MIYASHITA Y,GOOD A G.Contribution of the GABA shunt tohypoxia-induced alanine accumulation in roots of Arabidopsis thaliana[J].Plant Cell Physiology,2008,49(1):92.
[28]曹凤秋,刘国伟,王伟红,等.高等植物尿素代谢及转运的分子机理[J].植物学报,2009,44(3):274.CAO F Q,LIU G W,WANG W H,et al.Molecular mechanisms of urea metabolism and transport in higher plants[J].Chinese Bulletin of Botany,2009,44(3):274.
[29]TODD C D,TIPTON P A,BLEVINS D G,et al.Update on ureide degradation in legumes[J].Journal of Experimental Botany,2006,57(1):10.
[2]NICHOLSON J K,LINDON J C,HOLMES E.Metabonomics:Understanding the metabolic responses of living systems to pathophysiological stimuli via multivariate statistical analysis of biological NMR spectroscopic data[J].Xenobiotica,1999,29(11):1181.
[3]倪建伟,杨秀艳,张华新,等.代谢组学在植物逆境胁迫研究中的应用[J].世界林业研究,2014,27(5):12.NI J W,YANG X Y,ZHANG H X,et al.Metabolomics and its application to plant press research[J].World Forestry Research,2014,27(5):12.
[4]GRIMPLET J,WHEATLEY M D,JOUIRA H B,et al.Proteomic and selected metabolite analysis of grape berry tissues under well-watered and water-deficit stress conditions[J].Proteomics,2009,9(9):2507.
[5]RIZHSKY L,LIANG H,SHUMAN J,et al.The response of Arabidopsis to a combination of drought and heat stress[J].Plant Physiology,2004,134(4):1683.
[6]KEMPA S,KRASENSKY J,DALSANTO S,et al.A central role of abscisic acid in stress-regulated carbohydrate metabolism[J].PLos One,2008,3(12):3914.
[7]SUNG J K,LEE S,LEE Y J,et al.Metabolomic profiling from leaves and roots of tomato(Solanum lycopersicum L.)plants grown under nitrogen,phosphorus or potassium-deficient condition[J].Plant Science,2015(241):62.
[8]于晶,张林,崔红,等.高寒地区冬小麦东农冬麦1号越冬前的生理生化特性[J].作物学报,2008,34(11):2019.YU J,ZHANG L,CUI H,et al.Physiological and biochemical characteristics of Dongnongdongmai 1 before wintering in high-cold area[J].Acta Agronomica Sinica,2008,34(11):2019.
[9]KIND T,WOHLGEMUTH G,LEE D Y,et al.FiehnLibmass spectral and retention index libraries for metabolomics based on quadrupole and time-of-flight gas chromatography/mass spectrometry[J].Analytical Chemistry,2009,81(24):10040.
[10]BAGA M,CHODAPARAMBIL S V,LIMIN A E,et al.Identification of quantitative trait loci and associated candidate genes for low-temperature tolerance in cold-hardy winter wheat[J].Functional&Integrative Genomics,2007(7):53.
[11]吴冰,苍晶,张达,等.SA处理对低温胁迫下冬小麦(Triticum aestivum L.)蔗糖代谢的影响[J].植物生理学报,2015,51(4):537.WU B,CANG J,ZHANG D,et al.Effects of SA treatment on winter wheat(Triticum aestivum L.)sucrose metabolism in low temperature[J].Plant Physiology Journal,2015,51(4):537.
[12]ONEILL S D.Osmotic adjustment and the development of freezing resistance in Fragaria virginiana[J].Plant Physiology,1983,72(4):938.
[13]KINDL H,HOFFMAN-OSTENHOF O.Sudies on the biosynthesis of cyclitols,II.Formation of meso-inositol from 14C-1-glocose in sinapis alba and selective degradation of the resulting product[J].Biochemische Zeitschrift,1964,339(93):374.
[14]MAJUMDER A L,JOHNSON M D,HENRY S A.1L-myoInositol-1-phosphate synthase[J].Biochimica et Biophysica Acta-biomembranes,1997,1348(1/2):245.
[15]LOEWUS F A,MURTHYP P N.Myo-inositol metabolism in plants[J].Plant Science,2000,150(1):1.
[16]张梦,谢益民,杨海涛,等.肌醇在植物体内的代谢概述-肌醇作为细胞壁木聚糖和果胶前驱物的代谢途径[J].林产化学与工业,2013,33(5):109.ZHANG M,XIE Y M,YANG H T,et al.Myo-inositol metamolism as the precursor of xylan and pectin in plants[J].Chemistry and Industry of Forest Products,2013,33(5):109.
[17]ASHRAF M,HARRIS P J C.Potential biochemical indicators of salinity tolerance in plants[J].Plant Science,2004,166(1):6.
[18]徐宇,肖化云,郑能建,等.植物组织中游离氨基酸在盐胁迫下响应的研究进展[J].环境科学与技术,2016,39(7):41.XU Y,XIAO H Y,ZHENG N J,et al.Progress on responding of free amino acid in plants to salt stress[J].Environmental Science&Technology,2016,39(7):40.
[19]GHOULAM C,FOURSY A,FARES K.Effects of salt stress on growth,inorganic ions and proline accumulation in relation to osmotic adjustment in five sugar beet cultivars[J].Environmental and Experimental Botany,2002,47(1):39.
[20]KISHOR P K,SANGAM S,AMRUTHA R,et al.Regulation of proline biosynthesis,degradation,uptake and transport in higher plants:Its implications in plant growth and abiotic stress tolerance[J].Current Science,2005,88(3):424.
[21]全先庆,张渝洁,单雷,等.脯氨酸在植物生长和非生物胁迫耐受中的作用[J].生物技术通讯,2007,18(1):160.QUAN X Q,ZHANG Y J,SHAN L,et al.The roles of proline in plant growth and the tolerance to abiotic stresses[J].Letters in Biotechnology,2007,18(1):160.
[22]AL-QURAAN N A,LOCY R D,SINGH N K.Implications of paraquat and hydrogen peroxide-induced oxidative stress treatments on the GABA shunt pathway in Arabidopsis thaliana calmodulin mutants[J].Plant Biotechnoogyl Reports,2011,5(3):233.
[23]BOUCHE N,FAIT A,BOUCHEZ D,et al.Mitochondrial succinic-semialdehyde dehydrogenase of theγ-aminobutyrate shunt is required to restrict levels of reactive oxygen intermediates in plants[J].Proceedings of the National Academy of Sciences of the United States of America,2003,100(11):6848.
[24]LANCINE M,ROBERTS M R.Regulation of Arabidopsis thaliana 14-3-3gene expression by g-aminobutyric acid[J].Plant Cell Environment,2006,29(7):1434.
[25]SU G X,YU B J,ZHANG W H,et al.Higher accumulation of g-aminobutyric acid induced by salt stress through stimulating the activity of diamine oxidases in Glycine max(L.)Merr.roots[J].Plant Physiology and Biochemistry,2007,45(8):560.
[26]DITTAMI S M,GRAVOT A,RENAULT D,et al.Integrative analysis of metabolite and transcript abundance during the short-term response to saline and oxidative stress in the brown algaEctocarpus siliculosus[J].Plant Cell Environment,2011,34(4):629.
[27]MIYASHITA Y,GOOD A G.Contribution of the GABA shunt tohypoxia-induced alanine accumulation in roots of Arabidopsis thaliana[J].Plant Cell Physiology,2008,49(1):92.
[28]曹凤秋,刘国伟,王伟红,等.高等植物尿素代谢及转运的分子机理[J].植物学报,2009,44(3):274.CAO F Q,LIU G W,WANG W H,et al.Molecular mechanisms of urea metabolism and transport in higher plants[J].Chinese Bulletin of Botany,2009,44(3):274.
[29]TODD C D,TIPTON P A,BLEVINS D G,et al.Update on ureide degradation in legumes[J].Journal of Experimental Botany,2006,57(1):10.