熟肉制品中苯甲酸钠和山梨酸钾的快速同时测定
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摘要
建立一维混合紫外-可见光谱快速同时测定市售肉类熟食中苯甲酸钠和山梨酸钾含量的方法。首先进行分析模型数据库建立,包括苯甲酸钠和山梨酸钾的标准紫外-可见光谱数据库和样品本底数据库的建立。采集肉类熟食品样品提取液的紫外-可见光谱,调用分析模型数据库,基于向量-子空间夹角判据可同时计算肉类熟食品中苯甲酸钠和山梨酸钾的含量。实验结果表明,快速测定方法与高效液相色谱对比,相对误差小于12%,方法回收率在97.20%～107.60%,相对标准偏差小于3%,定量检测中效果良好,可应用于其他多组分复杂体系的快速检测分析。
A new method of rapid determination of sodium benzoate and potassium sorbate in cooked meat with one dimensional mixture spectrum was established. At first, analysis mode was established including spectrum databases of samples' background and standard solutions of sodium benzoate and potassium sorbate measured by UV-visible optical fiber spectrometer. Then the content of sodium benzoate and potassium sorbate were determined by such analysis mode based on vector-subspace angle criterion. The results showed that the relative errors were less than 12 % compared with HPLC, the recovery rates were 97.20 %～107.60 %with relative standard deviations less than 3 %. This method was effective in rapidly detecting of complex samples with one dimestional spectrum.
A new method of rapid determination of sodium benzoate and potassium sorbate in cooked meat with one dimensional mixture spectrum was established. At first, analysis mode was established including spectrum databases of samples' background and standard solutions of sodium benzoate and potassium sorbate measured by UV-visible optical fiber spectrometer. Then the content of sodium benzoate and potassium sorbate were determined by such analysis mode based on vector-subspace angle criterion. The results showed that the relative errors were less than 12 % compared with HPLC, the recovery rates were 97.20 %～107.60 %with relative standard deviations less than 3 %. This method was effective in rapidly detecting of complex samples with one dimestional spectrum.
引文
1张国文,潘军辉,王福民,等.主成分回归用于分光光度法同时测定6种食品添加剂[J].分析试验室,2007,26(7):52-56.
2陈昌云,丁爱芳,赵波,等.固相萃取-高效液相色谱法测定肉制品中山梨酸、苯甲酸含量[J].食品科学,2009,30(18):344-346.
3 SN/T 3545-2013,出口食品中多种防腐剂的测定方法[S].
4邓乐乐,张建铎,杨文武,等.香精中8种防腐剂的超高效液相色谱-串联质谱法测定[J].中国食品添加剂,2019,(1):156-160.
5粟晖,姚志湘,方凤,等.一种化妆品中对羟基苯甲酸酯的测定方法.中国专利:CN201310032832.7,2013-01-29.
6李登红,粟晖,姚志湘,等.植物油中3种抗氧化剂的同时快速分析[J].分析试验室,2016,35(1):61-65.
7杨向妮,姚志湘,粟晖,等.斜投影和空间夹角判据结合紫外光谱分析滴眼液中苯扎氯铵[J].分析测试学报,2016,35(3):337-341.
8闫一夫,姚志湘,刘柳,等.基于色谱-光谱仪联用和子空间夹角法分析丹皮酚含量[J].中成药,2016,38(1):114-117.
9粟晖,方凤,姚志湘,等.基于HPLC-UV与空间夹角判据的化妆品中对羟基苯甲酸酯分析[J].计算机与应用化学,2014,31(5):611-614.
10姚志湘,蹇华丽,刘焕彬.多变量统计分析中独立变量数目的判定方法[J].华南理工大学学报:自然科学版,2007,35(1):123-128.
11 QIA X,LUO R Y,ZHAO H Y.Sparse principal component analysisby choice of norm[J].Journal of Multivariate Analysis,2013,114:127-160.
12保罗·戈培林.化学计量学实用指南[M](第2版).吴海龙,康超,译.北京:科学出版社,2006:93,301.
2陈昌云,丁爱芳,赵波,等.固相萃取-高效液相色谱法测定肉制品中山梨酸、苯甲酸含量[J].食品科学,2009,30(18):344-346.
3 SN/T 3545-2013,出口食品中多种防腐剂的测定方法[S].
4邓乐乐,张建铎,杨文武,等.香精中8种防腐剂的超高效液相色谱-串联质谱法测定[J].中国食品添加剂,2019,(1):156-160.
5粟晖,姚志湘,方凤,等.一种化妆品中对羟基苯甲酸酯的测定方法.中国专利:CN201310032832.7,2013-01-29.
6李登红,粟晖,姚志湘,等.植物油中3种抗氧化剂的同时快速分析[J].分析试验室,2016,35(1):61-65.
7杨向妮,姚志湘,粟晖,等.斜投影和空间夹角判据结合紫外光谱分析滴眼液中苯扎氯铵[J].分析测试学报,2016,35(3):337-341.
8闫一夫,姚志湘,刘柳,等.基于色谱-光谱仪联用和子空间夹角法分析丹皮酚含量[J].中成药,2016,38(1):114-117.
9粟晖,方凤,姚志湘,等.基于HPLC-UV与空间夹角判据的化妆品中对羟基苯甲酸酯分析[J].计算机与应用化学,2014,31(5):611-614.
10姚志湘,蹇华丽,刘焕彬.多变量统计分析中独立变量数目的判定方法[J].华南理工大学学报:自然科学版,2007,35(1):123-128.
11 QIA X,LUO R Y,ZHAO H Y.Sparse principal component analysisby choice of norm[J].Journal of Multivariate Analysis,2013,114:127-160.
12保罗·戈培林.化学计量学实用指南[M](第2版).吴海龙,康超,译.北京:科学出版社,2006:93,301.