辽东楤木叶总皂苷高效薄层色谱指纹图谱分析
|
摘要
目的:建立辽东楤木叶总皂苷高效薄层色谱指纹图谱,并分析不同产地不同采收期的辽东楤木叶成分的差别。方法:采用高效硅胶G薄层板(20 cm×20 cm)载样,以三氯甲烷-甲醇-乙酸乙酯-甲酸-水(9. 5∶10∶20∶0. 5∶5)为展开剂,以10%浓硫酸乙醇溶液为显色剂,于恒温鼓风干燥箱里100℃条件下加热至斑点清晰,置于365 nm紫外光下照射获得其显色后的荧光高效薄层色谱图,经软件组合处理生成斑点灰度峰曲线,建立共有模式,并进行相似度分析及聚类分析。结果:获得分离度良好、斑点清晰的高效薄层色谱指纹图谱,建立了辽东楤木叶总皂苷薄层色谱指纹图谱共有模式,由10个共有斑点峰组成,并指认了其中的4个斑点峰代表的具体成分。分析表明不同地区8月初至9月中旬的辽东楤木叶相似度较高;聚类分析将11个不同批次的样品聚为一大类。结论:建立的薄层色谱指纹图谱方法简单、快速、可靠,可用于辽东楤木叶药材的鉴定和质量控制评价。确定东北地区8月采收的辽东楤木叶药材均符合质量要求,可作为以总皂苷为主要药效部位的药材使用。
Objective: To establish high performance thin layer chromatography( HPTLC) fingerprint of the total saponins from Aralia elata leaves,and compare the difference of components in A. elata leaves from different harvest time and different regions. Method: High efficiency silica gel G thin sheet( 20 cm × 20 cm) was used,with chloroform-methanol-ethyl acetate-water( 9. 5 ∶ 10 ∶ 20 ∶ 0. 5 ∶ 5) as developing system,ethanol solution of 10% concentrated sulfuric acid as chromogenic reagent,heating at 100 ℃ in constant-temperature air dry oven until clear spots. The fluorescence HPTLC chromatogram fingerprints were obtained under 365 nm ultraviolet light.Speckle patterns were obtained by software processing and the common pattern was established for similarity analysis and cluster analysis. Result: The HPTLC fingerprints with good separation and clear spots were obtained and the common pattern of fingerprints was established. The common pattern was composed of 10 common speckled peaks,4 of which were identified for components. The results showed that samples in early August to mid September from different regions had good similarity. 11 samples of different batches were clustered into one class. Conclusion:The HPTLC method is simple,fast and reliable,and can be used for the identification and quality evaluation of medicinal materials of A. elata leaves. The A. elata leaves collected in August conform to the quality standard,so they can be used as medicinal materials.
Objective: To establish high performance thin layer chromatography( HPTLC) fingerprint of the total saponins from Aralia elata leaves,and compare the difference of components in A. elata leaves from different harvest time and different regions. Method: High efficiency silica gel G thin sheet( 20 cm × 20 cm) was used,with chloroform-methanol-ethyl acetate-water( 9. 5 ∶ 10 ∶ 20 ∶ 0. 5 ∶ 5) as developing system,ethanol solution of 10% concentrated sulfuric acid as chromogenic reagent,heating at 100 ℃ in constant-temperature air dry oven until clear spots. The fluorescence HPTLC chromatogram fingerprints were obtained under 365 nm ultraviolet light.Speckle patterns were obtained by software processing and the common pattern was established for similarity analysis and cluster analysis. Result: The HPTLC fingerprints with good separation and clear spots were obtained and the common pattern of fingerprints was established. The common pattern was composed of 10 common speckled peaks,4 of which were identified for components. The results showed that samples in early August to mid September from different regions had good similarity. 11 samples of different batches were clustered into one class. Conclusion:The HPTLC method is simple,fast and reliable,and can be used for the identification and quality evaluation of medicinal materials of A. elata leaves. The A. elata leaves collected in August conform to the quality standard,so they can be used as medicinal materials.
引文
[1]中国科学院《中国植物志》编辑委员会.中国植物志[M].北京:科学出版社,1978:160-167.
[2]李书心.辽宁植物志[M].沈阳:辽宁科学技术出版社,1988:1268.
[3]国家中医药管理局《中华本草》编委会.中华本草[M].上海:上海科学技术出版社,1999:787-789.
[4]南京中医药大学.中药大辞典[M].上海:上海科学技术出版社,2006:885-886,1834-1835.
[5]邹淑君,许树军,李靖,等.辽东楤木叶皂苷成分及其药学研究进展[J].中医药学报,2017,45(4):111-114.
[6]王丽岩,肖洪彬,王鸣慧.辽东楤木叶总皂苷抗急性肝损伤的实验研究[J].中医药信息,2009,26(2):29-30,6.
[7]张秀萍,孙达辰,肖洪彬.辽东楤木叶总皂苷抗肿瘤作用研究进展[J].中国实验方剂学杂志,2015,21(11):232-234.
[8] LI F J,HE X L,NIU W Y,et al. Sub-chronic safety evaluation of the ethanol extract of Aralia elata leaves in Beagle dogs[J]. Regul Toxicol Pharm,2016(79):1-11.
[9]尹丽,宗兰兰,蒲晓辉,等.薄层色谱法在药物分析中的应用[J].河南大学学报:医学版,2016,35(2):77-80.
[10]冯雅斌,杜靓,温静.薄层色谱法在药物分析中的应用及研究进展[J].疾病监测与控制杂志,2011,5(1):60-63.
[11]姚令文,石岩,孙冬梅,等.人工牛黄薄层指纹图谱多元图像分析及化学计量学研究[J].中国中药杂志,2017,42(11):2117.
[12]邹纯才,鄢海燕.我国中药色谱指纹图谱相似度评价方法30年(1988—2017年)研究进展与展望[J].中国中药杂志,2018,43(10):1969-1977.
[13]高鹏,代龙.薄层色谱法在中药指纹图谱研究中的应用[J].山东医药工业,2002,21(6):17-19.
[2]李书心.辽宁植物志[M].沈阳:辽宁科学技术出版社,1988:1268.
[3]国家中医药管理局《中华本草》编委会.中华本草[M].上海:上海科学技术出版社,1999:787-789.
[4]南京中医药大学.中药大辞典[M].上海:上海科学技术出版社,2006:885-886,1834-1835.
[5]邹淑君,许树军,李靖,等.辽东楤木叶皂苷成分及其药学研究进展[J].中医药学报,2017,45(4):111-114.
[6]王丽岩,肖洪彬,王鸣慧.辽东楤木叶总皂苷抗急性肝损伤的实验研究[J].中医药信息,2009,26(2):29-30,6.
[7]张秀萍,孙达辰,肖洪彬.辽东楤木叶总皂苷抗肿瘤作用研究进展[J].中国实验方剂学杂志,2015,21(11):232-234.
[8] LI F J,HE X L,NIU W Y,et al. Sub-chronic safety evaluation of the ethanol extract of Aralia elata leaves in Beagle dogs[J]. Regul Toxicol Pharm,2016(79):1-11.
[9]尹丽,宗兰兰,蒲晓辉,等.薄层色谱法在药物分析中的应用[J].河南大学学报:医学版,2016,35(2):77-80.
[10]冯雅斌,杜靓,温静.薄层色谱法在药物分析中的应用及研究进展[J].疾病监测与控制杂志,2011,5(1):60-63.
[11]姚令文,石岩,孙冬梅,等.人工牛黄薄层指纹图谱多元图像分析及化学计量学研究[J].中国中药杂志,2017,42(11):2117.
[12]邹纯才,鄢海燕.我国中药色谱指纹图谱相似度评价方法30年(1988—2017年)研究进展与展望[J].中国中药杂志,2018,43(10):1969-1977.
[13]高鹏,代龙.薄层色谱法在中药指纹图谱研究中的应用[J].山东医药工业,2002,21(6):17-19.