燥湿止痒洗液质量标准提升研究
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摘要
目的探讨燥湿止痒洗液中黄芩、蛇床子的质量标准提升。方法采用薄层色谱法鉴别黄芩、蛇床子,从制备方法、展开剂、点样量、检视条件、专属性、温度、相对湿度、薄层板等影响因素中筛选最优的薄层色谱条件。结果黄芩采用硅胶G薄层板,以乙酸乙酯-丁酮-甲酸-水(6∶3∶2∶1)为展开剂,喷以2%三氯化铁乙醇溶液,晾干后检视显示斑点清晰;蛇床子采用硅胶G薄层板,以甲苯-甲醇-甲酸(10∶2∶1)为展开剂,于紫外灯365 nm下检视,荧光斑点明显。结论该方法操作简单、斑点清晰、重现性好、专属性强,阴性无干扰,可适用于该制剂的质量控制。
Objective To investigate the improvement of the quality standard of Scutellaria baicalensis and Cnidium monnieri in ZaoshiZhiyang Lotion. Methods TLC method was used to identify Scutellaria baicalensis and Cnidium monnieri. The optimization of TLCconditions was selected from the factors such as preparation methods, developing agent, sample size, inspection conditions, specificity,temperature,relative humidity,thin-layer plate and so on. Results The optimum conditions of Scutellaria baicalensis were as follows:using silica gel G thin-layer plate with ethyl acetate-butanone-formic acid-water( 6 ∶ 3 ∶ 2 ∶ 1) as the developing agent,spraying 2%ferric chloride ethanol solution,after air drying,the spots were clear. The optimum conditions of Cnidium monnieri were as follows: usingsilica gel G thin-layer plate with toluene-methanol-formic acid( 10 ∶ 2 ∶ 1) as the developing agent, the fluorescence spots wereobvious when examined under 365 nm UV lamp. Conclusion The method is simple, clear, reproducible,specific and has no negativeinterference,which can be used for the quality control of Zaoshi Zhiyang Lotion.
Objective To investigate the improvement of the quality standard of Scutellaria baicalensis and Cnidium monnieri in ZaoshiZhiyang Lotion. Methods TLC method was used to identify Scutellaria baicalensis and Cnidium monnieri. The optimization of TLCconditions was selected from the factors such as preparation methods, developing agent, sample size, inspection conditions, specificity,temperature,relative humidity,thin-layer plate and so on. Results The optimum conditions of Scutellaria baicalensis were as follows:using silica gel G thin-layer plate with ethyl acetate-butanone-formic acid-water( 6 ∶ 3 ∶ 2 ∶ 1) as the developing agent,spraying 2%ferric chloride ethanol solution,after air drying,the spots were clear. The optimum conditions of Cnidium monnieri were as follows: usingsilica gel G thin-layer plate with toluene-methanol-formic acid( 10 ∶ 2 ∶ 1) as the developing agent, the fluorescence spots wereobvious when examined under 365 nm UV lamp. Conclusion The method is simple, clear, reproducible,specific and has no negativeinterference,which can be used for the quality control of Zaoshi Zhiyang Lotion.
引文
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[2]辛文妤,宋俊科,何国荣,等.黄芩素和黄芩苷的药理作用及机制研究发展[J].中国新药杂志,2013,22(6):647-653.
[3]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药出版社,2015:315-316.
[4]赵莹莹,伍冠一,邵容格,等.中药成方制剂中蛇床子方剂的用药规律分析[J].中国民族民间医药,2017,26(16):9-12.
[5]郑立卿,张力,董晓华,等.蛇床子素药理作用研究进展[J].神经药理学报,2013,3(6):29-35.
[6]许佳楠,朱盛山,邓剑壕,等.中药复方NZ制剂中黄芩苷薄层鉴别及含量测定[J].亚太传统医药,2017,13(7):41-43.
[7]吴桐,张巧丽,姚树坤.清肝化瘀方质量标准研究[J].世界中医学,2017,12(10):2457-2461.
[8]高永丰,马艳玲,赵永亮,等.黄芩薄层色谱鉴别研究[J].北方药学,2013,10(2):6.
[9]马静,宋毅,费小凡,等.鼻窍通颗粒中黄芪、黄芩的薄层鉴别研究[J].华西医学,2017,32(9):1409-1413.
[10]李向军,王超,王永,等.中药薄层色谱影响因素分析及应用[J].中国药业,2011,20(14):13-15.
[11]韩晓霞.中药薄层色谱鉴别检验中的影响因素及问题探讨[J].医学信息,2014,10:452-453.
[12]国家药典委员会.中华人民共和国药典(四部)[M].北京:中国医药出版社,2015:57-58.
[13]张慧月,赵妹,赵雪梅.祛湿止痒洗液的定性定量方法研究[J].药物分析杂志,2017,37(5):903-910.
[14]石小鹏,缪珊,李捷,等.参蛇软膏的质量标准研究[J].西北药学杂志,2014,29(2):114-117.
[15]李江,王慧,左文,等.舒乐洗剂的薄层鉴别研究[J].中医药导报,2015,21(9):42-44.
[16]张静,胡代琼,刘三侠,等.常见甘草品种有效成分及药理作用研究进展[J].中兽医医药杂志,2012,31(1):23-27.
[17]李坤平,高崇凯,李卫民. UPLC/ESI-TOF-MS/MS分析蛇床子提取物中香豆素类化合物[J].中成药,2009,31(4):584-587.