蒙古红鲌形态特征及其两种同工酶的电泳分析
|
摘要
【目的】从形态特征和生化遗传两个角度丰富蒙古红鲌(Erythroculter mongolicus)种质资源的研究内容,筛选出蒙古红鲌的特征生化遗传参数,为其种质标准制定、种质资源保护及良种选育提供理论依据。【方法】采用传统形态学方法测定蒙古红鲌的可数和可量性状,并结合聚丙烯酰胺凝胶电泳(PAGE)检测蒙古红鲌心脏、眼睛晶状体、肌肉、肾脏及肝脏等5种组织中乳酸脱氢酶(LDH)和苹果酸脱氢酶(MDH)的表达情况。【结果】蒙古红鲌的主要形态特征:头部背面平坦,口端位,下颌略比上颌长;腹棱自腹鳍基部至肛门;身体上半部浅棕色、下部银白色,胸腹鳍浅黄色,尾鳍下叶鲜红色;鳍式为背鳍D.ⅲ-7和臀鳍A.ⅲ-19～22;齿式为2·4·5(4)/5(4)·4·2或2·4·4/5·3·2:左侧第一鳃弓外侧鳃耙数为18～20。从蒙古红鲌心脏、眼睛晶状体、肌肉、肾脏和肝脏组织中均能检测到LDH和MDH,但不同组织中的酶带数目和活性各不同,呈明显的组织特异性,其中,LDH酶带条数分别为6、6、6、7和8条,MDH酶带条数分别为3、3、5、5和4条。各样本蒙古红鲌眼睛晶状体的LDH酶带数及其活性基本相同,均呈单态,表达稳定,且分离效果好。【结论】LDH和MDH在蒙古红鲌体内分布较广泛,且具有组织特异性,其中眼睛晶状体LDH表达丰富且活性稳定,可作为鉴定蒙古红鲌种质的特征生化遗传参数。
【Objective】This paper aimed to enrich the research on the morphological and biochemical genetics characteristics of Erythroculter mongolicus and to screen out the biochemical genetic markers of E. mongolicus so as to provide theoretical basis for the germplasm standard formulation,protection and breeding.【Method】The countable and measurable properties of the experimental fish were measured with traditional morphological methods. By using the vertical polyacrylamide gel electrophoresis(PAGE),lactate dehydrogenase(LDH)and malate dehydrogenase(MDH)expressed in five tissues(heart,lens of eye,muscle,kidney and liver)from the E. mongolicus were detected.【Result】The results showed several main morphological characteristics as follows. The dorsum and head were straight and flat with a mouth locating at the foremost part of the head where an underjaw that protruded the upper jaw. The ventral edge was from the base of the abdominal fin to the anus. Color of the top half of the body was light brown and the lower half was silver white. In addition,the pectoral and abdominal fins were light yellow and the lower caudal fin was bright red. Furthermore,the dorsal fin formula was D.ⅲ-7 and anal fin A.ⅲ-19-22.The dental formula was 2·4·5(4)/5(4)·4·2 or 2·4·4/5·3·2 while the number of outer gill racker on the left first gill arch was 18-20. Both LDH and MDH were detected in the five tissues(heart,lens of eye,muscle,kidney and liver)from the E. mongolicus,but the band numbers and activities of the isozymes expressed in different tissues were different which presented tissue specificity. The band numbers of LDH isozymes expressed in heart,lens of eye,muscle,kidney and liver were 6,6,6,7 and 8 respectively and MDH isozymes 3,3,5,5 and 4 respectively. The band numbers and activity of LDH expressed in the lens of eyes from all the samples were basically the same,which showed monomorphic,good separation and expressed stably.【Conclusion】LDH and MDH distribute widely in E. mongolicus and present tissue specificity. LDH isozymes expressed in lens of eyes is diverse and stable,and can be used as the biochemical genetic marker of E. mongolicus.
【Objective】This paper aimed to enrich the research on the morphological and biochemical genetics characteristics of Erythroculter mongolicus and to screen out the biochemical genetic markers of E. mongolicus so as to provide theoretical basis for the germplasm standard formulation,protection and breeding.【Method】The countable and measurable properties of the experimental fish were measured with traditional morphological methods. By using the vertical polyacrylamide gel electrophoresis(PAGE),lactate dehydrogenase(LDH)and malate dehydrogenase(MDH)expressed in five tissues(heart,lens of eye,muscle,kidney and liver)from the E. mongolicus were detected.【Result】The results showed several main morphological characteristics as follows. The dorsum and head were straight and flat with a mouth locating at the foremost part of the head where an underjaw that protruded the upper jaw. The ventral edge was from the base of the abdominal fin to the anus. Color of the top half of the body was light brown and the lower half was silver white. In addition,the pectoral and abdominal fins were light yellow and the lower caudal fin was bright red. Furthermore,the dorsal fin formula was D.ⅲ-7 and anal fin A.ⅲ-19-22.The dental formula was 2·4·5(4)/5(4)·4·2 or 2·4·4/5·3·2 while the number of outer gill racker on the left first gill arch was 18-20. Both LDH and MDH were detected in the five tissues(heart,lens of eye,muscle,kidney and liver)from the E. mongolicus,but the band numbers and activities of the isozymes expressed in different tissues were different which presented tissue specificity. The band numbers of LDH isozymes expressed in heart,lens of eye,muscle,kidney and liver were 6,6,6,7 and 8 respectively and MDH isozymes 3,3,5,5 and 4 respectively. The band numbers and activity of LDH expressed in the lens of eyes from all the samples were basically the same,which showed monomorphic,good separation and expressed stably.【Conclusion】LDH and MDH distribute widely in E. mongolicus and present tissue specificity. LDH isozymes expressed in lens of eyes is diverse and stable,and can be used as the biochemical genetic marker of E. mongolicus.
引文
陈清华,刘伟,唐富江.2011.兴凯湖4种野生鲌类营养成分的比较分析[J].大连海洋大学学报,26(1):88-92.[Chen Q H,Liu W,Tang F J.2011.Comparison of nutritive component among four wild culters in Xingkai Lake[J].Journal of Dalian Ocean University,26(1):88-92.]
丁严冬,藏雪,张国松,汪亚媛,陈树桥,王佩佩,刘炜,周国勤,尹绍武.2015.河川沙塘鳢4个不同地理群体的形态差异分析[J].海洋渔业,37(1):24-30.[Ding Y D,Zang X,Zhang G S,Wang Y Y,Chen S Q,Wang P P,Liu W,Zhou G Q,Yin S W.2015.Analysis of morphological variations among four different geographic populations of Odontobutis potamophila[J].Marine Fisheries,37(1):24-30.]
冯为慧,官少飞,欧阳敏.2012.刺鲃同工酶的研究[J].安徽农业科学,40(7):4029-4031.[Feng W H,Guan S F,Ouyang M.2012.Study on isozyme of Barbodes caldwelli[J].Journal of Anhui Agricultural Sciences,40(7):4029-4031.]
冯晓宇,郭水荣,李行先,王宇希,马柏林.2007.蒙古红鲌池塘高产养殖试验[J].科学养鱼,(3):30-31.[Feng X Y,Guo S R,Li X X,Wang Y X,Ma B L.2007.Study on high production cultivation of Erythroculter mongolicus in pools[J].Scientific Fish Farming,(3):30-31.]
冯晓宇,谢楠,李行先,许宝青.2008.红鲌属3种鱼类线粒体细胞色素b基因片段序列分析[J].西南大学学报(自然科学版),30(10):79-83.[Feng X Y,Xie N,Li X X,Xu B Q.2008.Sequence analysis of mitochondrial Cytb gene fragments of three species of Erythroculter[J].Journal of Southwest University(Natural Science Edition),30(10):79-83.]
戈志强,宋学宏,许爱国,展龙平,汤晓红,严智瑜.2005.红鲌属3种鲌鱼同工酶的比较研究[J].水利渔业,25(6):23-24.[Ge Z Q,Song X H,Xu A G,Zhan L P,Tang X H,Yan Z Y.2005.Comparison of isozymes from three species of Erythroculter[J].Reservoir Fisheries,25(6):23-24.]
广西壮族自治区水产研究所,中国科学院动物研究所.2006.广西淡水鱼类志[M].第2版.南宁:广西人民出版社:171-173.[Guangxi Institute of Fisheries,Institute of Zoology,Chinese Academy of Science.2006.The Freshwater Fishes of Guangxi Zhuang Autonomous Region[M].The2ndEdition.Nanning:Guangxi People Publishing House:171-173.]
胡海彦,狄瑜,赵永锋,宋迁红,邴旭文.2011.蠡湖4种鲌鱼形态特征的比较研究[J].云南农业大学学报,26(4):488-494.[Hu H Y,Di Y,Zhao Y F,Song Q H,Bing X W.2011.Comparative study on the morphological of characteristics of four species of Culter and Culterichthys in Lihu Lake[J].Journal of Yunnan Agricultural University,26(4):488-494.]
湖南省水产科学研究所.1977.湖南鱼类志[M].长沙:湖南人民出版社:60-62.[Hunan Fisheries Science Institute.1977.The Fishes in Hunan[M].Changsha:Hunan People’s Publishing House:60-62.]
蒋晓华,昝瑞光,谷甦.1996.云南滇池高背鲫鱼乳酸脱氢酶(LDH)同工酶发育遗传学分析[J].西南农业学报,9(4):53-57.[Jiang X H,Zan R G,Gu S.1996.Analysis on the developmental genetics of lactate dehydrogenase isozymes in Dianchi high-back gructian carp(Carassius Auratus)[J].Southwest China Journal of Agriculture Science,9(4):53-57.]
李世华.2006.蒙古红鲌生物学特性及其养殖技术[J].中国水产,(10):22-23.[Li S H.2006.Biological characteristics and cultivation technology on Erythroculter mongolicus[J].China Fisheries,(10):22-23.]
林明利,李钟杰,夏雨果,王齐东.2013.蒙古鲌鱼食性驯化及鱼种培育初步研究[J].水生生物学报,37(5):974-977.[Lin M L,Li Z J,Xia Y G,Wang Q D.2013.Preliminary study on feeding domestication and fingerling cultivation of Culter mongolicus[J].Acta Hydrobiological Sinica,37(5):974-977.]
刘恩生,吴林坤,曹萍,丁淑荃,杨启超.2007.太湖鲌鱼数量变化规律及生态效应分析[J].水利渔业,27(3):70-73.[Liu E S,Wu L K,Cao P,Ding S Q,Yang Q C.2007.Change law of population and ecological effect analysis of Erythroculter mongolicus in Taihu Lake[J].Reservoir Fisheries,27(3):70-73.]
刘伟,陈清华,唐富江,李培伦,王继隆.2017.兴凯湖四种野生鲌消化酶活性比较研究[J].水产学杂志,30(5):23-27.[Liu W,Chen Q H,Tang F J,Li P L,Wang J L.2017.Comparison of activities of digestive enzymes among four culter species in Xingkai Lake[J].Chinese Journal of Fisheries,30(5):23-27.]
龙华,邓怀,邹世平.2000.欧洲鳗与日本鳗的同工酶鉴别研究[J].湖北农学院学报,20(1):68-71.[Long H,Deng H,Zou S P.2000.Studies on the isozymes differentiation of European eel and Japanese eel[J].Journal of Hubei Agriculture College,20(1):68-71.]
潘开宇,许俊,王振伟.2009.泼河水库蒙古红鲌生长参数的初步研究[J].信阳农业高等专科学校学报,19(1):116-117.[Pan K Y,Xu J,Wang Z W.2009.Preliminary study on growth parameters of Erythroculter mongolicus in Pohe Recervoir[J].Journal of Xinyang Agricultural College,19(1):116-117.]
唐章生.2001.巴西鲷乳酸脱氢酶同工酶的初步研究[J].水利渔业,21(4):9-10.[Tang Z S.2001.Preliminary study on lactate dehydrogenase of Prichilodws scrofa[J].Reservoir Fisheries,21(4):9-10.]
伍献文.1964.中国鲤科鱼类志(上卷)[M].上海:上海科学技术出版社:100-101.[Wu X W.1964.The Cyprinid Fishes in China(Volumn 1)[M].Shanghai:Shanghai Scientific&Technical Publishers:100-101.]
徐伟,耿龙武,贾钟贺,李池陶,尹家胜,张春波.2009.镜泊湖蒙古鲌人工繁育技术初步研究[J].淡水渔业,39(4):63-66.[Xu W,Geng L W,Jia Z H,Li C T,Yin J S,Zhang C B.2009.Preliminary studies on artificial propagation of Culter mongolicus in Jingpo Lake[J].Freshwater Fisheries,39(4):63-66.]
杨杰静,刘友明,熊善柏,赵思明.2012.梁子湖地区蒙古红鲌肌肉营养成分分析与评价[J].营养学报,34(2):199-200.[Yang J J,Liu Y M,Xiong S B,Zhao S M.2012.The nutritional composition and evaluation of muscles of Erythroculter mongolicus in Liangzihu[J].Acta Nutrimenta Sinica,34(2):199-200.]
张娟,王红叶,蔡焰值,卢涛,秦晓燕.2011.中华倒刺鲃同工酶组织特异性研究[J].湖北农业科学,50(24):5206-5210.[Zhang J,Wang H Y,Cai Y Z,Lu T,Qin X Y.2011.Study on isozymic tissue specificity of Spinibarbus sinensis[J].Hubei Agricultural Sciences,50(24):5206-5210.]
张龙岗,刘羽清,朱树人,杨玲,付佩胜,朱永安.2017.乌鳢和杂交鳢4种同工酶的比较研究[J].长江大学学报(自然版),14(18):33-36.[Zhang L G,Liu Y Q,Zhu S R,Yang L,Fu P S,Zhu Y A.2017.Comparative studies on the isozymes of Ophicephalus argus and Ophicephalus argus(♂)×Channa maeulata(♀)[J].Journal of Yangtze University(Natural Science Edition),14(18):33-36.]
张胜宇.2007.湖泊人工增殖放流品种选择与放流技术[J].江西水产科技,(2):2-6.[Zhang S Y.2007.Species selection and technology about artificial proliferation and release in lakes[J].Jiangxi Fishery Science and Technology,(2):2-6.]
张涛,陈建武,张林,周剑光,何力.2017.赤眼鳟形态特征及其两种同工酶的组织特异性分析[J].南方农业学报,48(12):2281-2287.[Zhang T,Chen J W,Zhang L,Zhou JG,He L.2017.Morphological characteristics of Squaliobarbus curriculus and tissue specificity analysis for two isozymes[J].Journal of Southern Agriculture,48(12):2281-2287.]
张涛,周剑光,何力.2018.双须叶须鱼5种组织乳酸脱氢酶和苹果酸脱氢酶的组织特异性分析[J].安徽农业大学学报,45(2):242-246.[Zhang T,Zhou J G,He L.2018.Preliminary study on lactate dehydrogenase(LDH)and malate dehydrogenase(MDH)isozymes in five different tissues in Ptychobarbus dipogon[J].Journal of Anhui Agricultural University,45(2):242-246.]
张小谷,杨建远,田小雪,熊邦喜,王亮.2008.4种鲌脑形态度量的比较研究[J].吉林农业大学学报,30(6):866-870.[Zhang X G,Yang J Y,Tian X X,Xiong B X,Wang L.2008.Comparative studies on brain morphometrics of four species of Culter[J].Journal of Jilin Agricultural University,30(6):866-870.]
张小立,杨品红,徐黎明,王晓艳.2008.翘嘴鲌、蒙古鲌形态特征及年龄与生长的研究[J].内陆水产,(9):7-10.[Zhang X L,Yang P H,Xu L M,Wang X Y.2008.Preliminary studies on age,growth and morphological characteristics of Culter alburnus and Culter mongolicus[J].Inland Fisheries,(9):7-10.]
周洲,李建光,王艳艳,孔杰,曾圣.2017.清水江鲤外部形态性状对体质量的影响[J].河南农业科学,46(12):134-138.[Zhou Z,Li J G,Wang Y Y,Kong J,Zeng S.2017.Mathematical analysis of morphometric traits effects on body weight for Qingshui River Cyprinus carpio[J].Journal of Henan Agricultural Sciences,46(12):134-138.]
Goli A,Rogelio D R,Reyes C R,Laude R P.2014.Genetics diversity of two Philippine native freshwater goby species(Perciformes:Gobiidae):Implications for conservation[J].Aquatic Conservation,24(5):592-600.
丁严冬,藏雪,张国松,汪亚媛,陈树桥,王佩佩,刘炜,周国勤,尹绍武.2015.河川沙塘鳢4个不同地理群体的形态差异分析[J].海洋渔业,37(1):24-30.[Ding Y D,Zang X,Zhang G S,Wang Y Y,Chen S Q,Wang P P,Liu W,Zhou G Q,Yin S W.2015.Analysis of morphological variations among four different geographic populations of Odontobutis potamophila[J].Marine Fisheries,37(1):24-30.]
冯为慧,官少飞,欧阳敏.2012.刺鲃同工酶的研究[J].安徽农业科学,40(7):4029-4031.[Feng W H,Guan S F,Ouyang M.2012.Study on isozyme of Barbodes caldwelli[J].Journal of Anhui Agricultural Sciences,40(7):4029-4031.]
冯晓宇,郭水荣,李行先,王宇希,马柏林.2007.蒙古红鲌池塘高产养殖试验[J].科学养鱼,(3):30-31.[Feng X Y,Guo S R,Li X X,Wang Y X,Ma B L.2007.Study on high production cultivation of Erythroculter mongolicus in pools[J].Scientific Fish Farming,(3):30-31.]
冯晓宇,谢楠,李行先,许宝青.2008.红鲌属3种鱼类线粒体细胞色素b基因片段序列分析[J].西南大学学报(自然科学版),30(10):79-83.[Feng X Y,Xie N,Li X X,Xu B Q.2008.Sequence analysis of mitochondrial Cytb gene fragments of three species of Erythroculter[J].Journal of Southwest University(Natural Science Edition),30(10):79-83.]
戈志强,宋学宏,许爱国,展龙平,汤晓红,严智瑜.2005.红鲌属3种鲌鱼同工酶的比较研究[J].水利渔业,25(6):23-24.[Ge Z Q,Song X H,Xu A G,Zhan L P,Tang X H,Yan Z Y.2005.Comparison of isozymes from three species of Erythroculter[J].Reservoir Fisheries,25(6):23-24.]
广西壮族自治区水产研究所,中国科学院动物研究所.2006.广西淡水鱼类志[M].第2版.南宁:广西人民出版社:171-173.[Guangxi Institute of Fisheries,Institute of Zoology,Chinese Academy of Science.2006.The Freshwater Fishes of Guangxi Zhuang Autonomous Region[M].The2ndEdition.Nanning:Guangxi People Publishing House:171-173.]
胡海彦,狄瑜,赵永锋,宋迁红,邴旭文.2011.蠡湖4种鲌鱼形态特征的比较研究[J].云南农业大学学报,26(4):488-494.[Hu H Y,Di Y,Zhao Y F,Song Q H,Bing X W.2011.Comparative study on the morphological of characteristics of four species of Culter and Culterichthys in Lihu Lake[J].Journal of Yunnan Agricultural University,26(4):488-494.]
湖南省水产科学研究所.1977.湖南鱼类志[M].长沙:湖南人民出版社:60-62.[Hunan Fisheries Science Institute.1977.The Fishes in Hunan[M].Changsha:Hunan People’s Publishing House:60-62.]
蒋晓华,昝瑞光,谷甦.1996.云南滇池高背鲫鱼乳酸脱氢酶(LDH)同工酶发育遗传学分析[J].西南农业学报,9(4):53-57.[Jiang X H,Zan R G,Gu S.1996.Analysis on the developmental genetics of lactate dehydrogenase isozymes in Dianchi high-back gructian carp(Carassius Auratus)[J].Southwest China Journal of Agriculture Science,9(4):53-57.]
李世华.2006.蒙古红鲌生物学特性及其养殖技术[J].中国水产,(10):22-23.[Li S H.2006.Biological characteristics and cultivation technology on Erythroculter mongolicus[J].China Fisheries,(10):22-23.]
林明利,李钟杰,夏雨果,王齐东.2013.蒙古鲌鱼食性驯化及鱼种培育初步研究[J].水生生物学报,37(5):974-977.[Lin M L,Li Z J,Xia Y G,Wang Q D.2013.Preliminary study on feeding domestication and fingerling cultivation of Culter mongolicus[J].Acta Hydrobiological Sinica,37(5):974-977.]
刘恩生,吴林坤,曹萍,丁淑荃,杨启超.2007.太湖鲌鱼数量变化规律及生态效应分析[J].水利渔业,27(3):70-73.[Liu E S,Wu L K,Cao P,Ding S Q,Yang Q C.2007.Change law of population and ecological effect analysis of Erythroculter mongolicus in Taihu Lake[J].Reservoir Fisheries,27(3):70-73.]
刘伟,陈清华,唐富江,李培伦,王继隆.2017.兴凯湖四种野生鲌消化酶活性比较研究[J].水产学杂志,30(5):23-27.[Liu W,Chen Q H,Tang F J,Li P L,Wang J L.2017.Comparison of activities of digestive enzymes among four culter species in Xingkai Lake[J].Chinese Journal of Fisheries,30(5):23-27.]
龙华,邓怀,邹世平.2000.欧洲鳗与日本鳗的同工酶鉴别研究[J].湖北农学院学报,20(1):68-71.[Long H,Deng H,Zou S P.2000.Studies on the isozymes differentiation of European eel and Japanese eel[J].Journal of Hubei Agriculture College,20(1):68-71.]
潘开宇,许俊,王振伟.2009.泼河水库蒙古红鲌生长参数的初步研究[J].信阳农业高等专科学校学报,19(1):116-117.[Pan K Y,Xu J,Wang Z W.2009.Preliminary study on growth parameters of Erythroculter mongolicus in Pohe Recervoir[J].Journal of Xinyang Agricultural College,19(1):116-117.]
唐章生.2001.巴西鲷乳酸脱氢酶同工酶的初步研究[J].水利渔业,21(4):9-10.[Tang Z S.2001.Preliminary study on lactate dehydrogenase of Prichilodws scrofa[J].Reservoir Fisheries,21(4):9-10.]
伍献文.1964.中国鲤科鱼类志(上卷)[M].上海:上海科学技术出版社:100-101.[Wu X W.1964.The Cyprinid Fishes in China(Volumn 1)[M].Shanghai:Shanghai Scientific&Technical Publishers:100-101.]
徐伟,耿龙武,贾钟贺,李池陶,尹家胜,张春波.2009.镜泊湖蒙古鲌人工繁育技术初步研究[J].淡水渔业,39(4):63-66.[Xu W,Geng L W,Jia Z H,Li C T,Yin J S,Zhang C B.2009.Preliminary studies on artificial propagation of Culter mongolicus in Jingpo Lake[J].Freshwater Fisheries,39(4):63-66.]
杨杰静,刘友明,熊善柏,赵思明.2012.梁子湖地区蒙古红鲌肌肉营养成分分析与评价[J].营养学报,34(2):199-200.[Yang J J,Liu Y M,Xiong S B,Zhao S M.2012.The nutritional composition and evaluation of muscles of Erythroculter mongolicus in Liangzihu[J].Acta Nutrimenta Sinica,34(2):199-200.]
张娟,王红叶,蔡焰值,卢涛,秦晓燕.2011.中华倒刺鲃同工酶组织特异性研究[J].湖北农业科学,50(24):5206-5210.[Zhang J,Wang H Y,Cai Y Z,Lu T,Qin X Y.2011.Study on isozymic tissue specificity of Spinibarbus sinensis[J].Hubei Agricultural Sciences,50(24):5206-5210.]
张龙岗,刘羽清,朱树人,杨玲,付佩胜,朱永安.2017.乌鳢和杂交鳢4种同工酶的比较研究[J].长江大学学报(自然版),14(18):33-36.[Zhang L G,Liu Y Q,Zhu S R,Yang L,Fu P S,Zhu Y A.2017.Comparative studies on the isozymes of Ophicephalus argus and Ophicephalus argus(♂)×Channa maeulata(♀)[J].Journal of Yangtze University(Natural Science Edition),14(18):33-36.]
张胜宇.2007.湖泊人工增殖放流品种选择与放流技术[J].江西水产科技,(2):2-6.[Zhang S Y.2007.Species selection and technology about artificial proliferation and release in lakes[J].Jiangxi Fishery Science and Technology,(2):2-6.]
张涛,陈建武,张林,周剑光,何力.2017.赤眼鳟形态特征及其两种同工酶的组织特异性分析[J].南方农业学报,48(12):2281-2287.[Zhang T,Chen J W,Zhang L,Zhou JG,He L.2017.Morphological characteristics of Squaliobarbus curriculus and tissue specificity analysis for two isozymes[J].Journal of Southern Agriculture,48(12):2281-2287.]
张涛,周剑光,何力.2018.双须叶须鱼5种组织乳酸脱氢酶和苹果酸脱氢酶的组织特异性分析[J].安徽农业大学学报,45(2):242-246.[Zhang T,Zhou J G,He L.2018.Preliminary study on lactate dehydrogenase(LDH)and malate dehydrogenase(MDH)isozymes in five different tissues in Ptychobarbus dipogon[J].Journal of Anhui Agricultural University,45(2):242-246.]
张小谷,杨建远,田小雪,熊邦喜,王亮.2008.4种鲌脑形态度量的比较研究[J].吉林农业大学学报,30(6):866-870.[Zhang X G,Yang J Y,Tian X X,Xiong B X,Wang L.2008.Comparative studies on brain morphometrics of four species of Culter[J].Journal of Jilin Agricultural University,30(6):866-870.]
张小立,杨品红,徐黎明,王晓艳.2008.翘嘴鲌、蒙古鲌形态特征及年龄与生长的研究[J].内陆水产,(9):7-10.[Zhang X L,Yang P H,Xu L M,Wang X Y.2008.Preliminary studies on age,growth and morphological characteristics of Culter alburnus and Culter mongolicus[J].Inland Fisheries,(9):7-10.]
周洲,李建光,王艳艳,孔杰,曾圣.2017.清水江鲤外部形态性状对体质量的影响[J].河南农业科学,46(12):134-138.[Zhou Z,Li J G,Wang Y Y,Kong J,Zeng S.2017.Mathematical analysis of morphometric traits effects on body weight for Qingshui River Cyprinus carpio[J].Journal of Henan Agricultural Sciences,46(12):134-138.]
Goli A,Rogelio D R,Reyes C R,Laude R P.2014.Genetics diversity of two Philippine native freshwater goby species(Perciformes:Gobiidae):Implications for conservation[J].Aquatic Conservation,24(5):592-600.