家蚕微孢子虫分子生物学研究进展
摘要
家蚕微孢子虫是家蚕微粒子病的病原,具有食下传染和胚胎传染两种途径,对蚕业生产具有毁灭性危害。随着分子生物学技术的发展,有关家蚕微孢子虫的分子生物学研究也取得重大进展。从家蚕微孢子虫的分子生物学检测、基因序列分析、侵染相关基因等方面进行论述,为家蚕微孢子虫不同功能基因的挖掘提供科学依据,为进一步阐明家蚕微孢子虫与宿主家蚕的侵染机制提供理论基础。
引文
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[14]刘吉平,曹阳,SMITH J E,等.模拟感染家蚕微粒子病的PCR分子诊断技术研究[J].中国农业科学,2004,37(12):1925-1931.
[15]HATAKEYAMAY,HAYASAKA S.A new method of pebrine inspection of silkworm egg using multiprimer PCR[J].Journal of Invertebrate Pathology,2003,82(3):148-151.
[16]何永强,吴姗,鲁兴萌,等.不同DNA抽提方法对普通PCR和实时荧光定量PCR方法检测家蚕微孢子虫的影响[J].昆虫学报,2011(11):1329-1334.
[17]何永强,吴姗,鲁兴萌,等.家蚕微孢子虫荧光定量PCR检测方法及诊断试剂盒[J].蚕业科学,2011,37(2):260-265.
[18]米锐,冀万杰,赵振军,等.实时荧光定量PCR检测柞蚕微孢子虫的方法[J].蚕业科学,2017,43(2):260-267.
[19]扈鸿霞,马宇轩,王艳红,等.蝗虫微孢子虫套式PCR检测方法的建立及应用[J].中国农业科学,2016,49(21):4239-4245.
[20]NOTOMI T,OKAYAMA H,MASUBUCHI H,et al.Loop-mediated isothermal amplification of DNA[J/OL].Nucleic Acids Research,2000,28(12):E63[2019-01-08].https://www.ncbi.nlm.nih.gov/pmc/articles/MC102748/?tdsourcetag=s_pcqq_aiomsg.
[21]SINGH P,MIRDHA B R,AHUJA V,et al.Loop-mediated isothermal amplification(LAMP)assay for rapid detection of Entamoeba histolytica in amoebic liver abscess[J].World Journal of Microbiology&Biotechnology,2013,29(1):27.
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[23]YAN W,SHEN Z,TANG X,et al.Detection of Nosema bombycis by FTA Cards and Loop-Mediated Isothermal Amplification(LAMP)[J].Current Microbiology,2014,69(4):532-540.
[24]刘吉平,杨吉龙.一种家蚕病原微孢子虫LAMP可视化快速检测试剂盒及其检测方法:CN,201310026870.1[P].2013-01-24.
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