海水胁迫对冰菜细胞损伤和有机小分子渗透调节物质积累影响
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摘要
【目的】探究海水胁迫对冰菜细胞损伤和脯氨酸、可溶性糖、甜菜碱积累影响。【方法】以冰菜为材料,采用盆栽试验研究不同浓度海水(20%、30%、40%、50%、60%)胁迫下冰菜细胞损伤(MDA、细胞膜透性)和有机小分子渗透调节物质(可溶性糖、甜菜碱、脯氨酸)的变化。【结果】随海水浓度增加,冰菜各级分枝细胞膜透性增强、可溶性糖含量增加;各级分枝MDA、甜菜碱含量变化为一级、二级分枝增加,三级分枝则先增加后减少;各级分枝脯氨酸含量变化均为先增加后减少。海水胁迫与细胞膜透性、可溶性糖含量呈显著正相关。各有机小分子渗透调节物质中仅可溶性糖与海水浓度和膜脂过氧化呈显著正相关。【结论】随着海水浓度升高,细胞膜受损伤加重。可溶性糖、甜菜碱、脯氨酸在不同浓度海水和不同生长时期起着重要渗透调节作用,且与海水胁迫相关性表现为可溶性糖>甜菜碱>脯氨酸。
【Objective】The objective is to explore the cumulative effects of seawater stress on the cell damage of Mesembryanthemum crystallinum L., proline, soluble sugar and betaine.【Method】A pot experiment was conducted on M.crystallinum L. to study the changes of cells damage(MDA and cell membrane permeability) and small organic molecular osmoregulation substances(soluble sugar, betaine and proline) with seawater stress at different concentrations(20%,30%, 40%, 50%, 60%). 【Result】With the increase of seawater concentration: the permeability of cell membranes and the content of soluble sugar were enhanced in each branch of M. crystallinum L.;MDA and the content of betaine increasedin the first and second branches, and increased and then decreased in tertiary branches; and the content of proline increased and then decreased in all branches. A significant positive correlation was discovered between seawater stress and the permeability of cell membranes and the content of soluble sugar. For small organic molecules osmoregulation substances, only the content of soluble sugar had a significant positive correlation with concentration of seawater and lipid peroxidation of membranes.【Conclusion】With the increase of seawater concentration, the damage of M. crystallinum L. cell membrane is aggravated. Soluble sugar, betaine and proline play an important role in osmotic regulation in different seawater concentrations and different growth stages. Moreover, their correlationwith seawater stress is as follows: soluble sugar > betaine > proline.
【Objective】The objective is to explore the cumulative effects of seawater stress on the cell damage of Mesembryanthemum crystallinum L., proline, soluble sugar and betaine.【Method】A pot experiment was conducted on M.crystallinum L. to study the changes of cells damage(MDA and cell membrane permeability) and small organic molecular osmoregulation substances(soluble sugar, betaine and proline) with seawater stress at different concentrations(20%,30%, 40%, 50%, 60%). 【Result】With the increase of seawater concentration: the permeability of cell membranes and the content of soluble sugar were enhanced in each branch of M. crystallinum L.;MDA and the content of betaine increasedin the first and second branches, and increased and then decreased in tertiary branches; and the content of proline increased and then decreased in all branches. A significant positive correlation was discovered between seawater stress and the permeability of cell membranes and the content of soluble sugar. For small organic molecules osmoregulation substances, only the content of soluble sugar had a significant positive correlation with concentration of seawater and lipid peroxidation of membranes.【Conclusion】With the increase of seawater concentration, the damage of M. crystallinum L. cell membrane is aggravated. Soluble sugar, betaine and proline play an important role in osmotic regulation in different seawater concentrations and different growth stages. Moreover, their correlationwith seawater stress is as follows: soluble sugar > betaine > proline.
引文
[1]王娟,李德全.逆境条件下植物体内渗透调节物质的积累与活性氧代谢[J].植物学通报,2001,18(4):459-465.WANG J,LI D Q.The Accumulation of plant osmoticum and activated oxygen metabolism under stress[J].Chinese Bulletin of Botany,2001,18(4):459-465.
[2]陈宏毅.冰菜的生物学特性与栽培技术[J].蔬菜,2016(8):42-45.CHEN H Y.Biological characteristics and cultivation techniques of Mesembryanthemum crystallinum L[J].Vegetables,2016(8):42-45.
[3]汪李平.长江流域塑料大棚冰菜栽培技术[J].长江蔬菜,2018(12):21-24.WANG L P.Cultivation Techniques of Mesembryanthemum crystallinum L.in plastic greenhouse in YangtzeRiver Basin[J].Journal of Changjiang Vegetables,2018(12):21-24.
[4]韩明丽,沈卫新,赵根,陈丽萍.保健蔬菜冰菜的生物学特性及关键栽培技术[J].湖南农业科学,2018(2):31-33.HAN M L,SHEN W X,ZHAO G,CHEN L P.The Biological characteristics and key cultivation techniques of healthy vegetable Mesembryanthemum crystallinum L[J].Hunan Agricultural Sciences,2018(2):31-33.
[5]IRENEUSZ S,MARTA L,ZBIGNIEW M.The foliar concentration of hydrogen peroxide during salt-induced Ca-CAM transition in Mesembryanthemum crystallinum L.[J].Plant Science,2008,174(2):221-226.
[6]AGARIE S,SHIMODA T,SHIMIZU Y,BAUMANN K,SUNAGAWA H,KONDO A,Ueno O,NAKAHARA T,NOSE A,CUSHMAN J C.Salt tolerance,salt accumulation,and ionic homeostasis in an epidermal bladder-cell-less mutant of the common ice plant Mesembryanthemum crystallinum[J].Journal of experimental botany,2007,58(8):1957-1967.
[7]徐微风,覃和业,刘姣,王蕾,吕瑞,郭建春,符少萍,李瑞梅,胡新文,段瑞军.冰菜在不同浓度海水胁迫下的氧化胁迫和抗氧化酶活性变化[J].江苏农业学报,2017,33(4):775-781.XU W F,QIN H Y,LIU J,WANG L,LV R,GUO J C,FU S P,LI R M,HUX W,DUAN R J.Changes of oxidative stress and antioxidant enzyme activity of Mesembryanthe-mum crystallinum Linnaeus in response to different concentrations of seawater[J].Jiangsu Journal of Agricultural Sciences,2017,33(4):775-781.
[8]郑炳松.现代植物生理生化研究技术[M].北京:气象出版社,2006.ZHENG B S.Modern Plant Physiological and Biochemical Research Technology[M].Beijing:China Meteorological Press,2006.
[9]陈建勋,王晓峰.植物生理学实验指导[M].第2版.广州:华南理工大学出版社,2006.CHEN J X,WANG X F.Guide to Plant Physiology Experiment[M].2nd Edition.Guangzhou:SouthChina University of Technology Press,2006.
[10]张宪政.作物生理研究法[M].北京:农业出版社,1992:129-150.ZHANG X Z.Crop physiology research method[M].Bei jing:Agricultural Press,1992:129-150.
[11]郭培国,宋波龙,许兰桂,郑岩松,李荣华,张华,黄红娣.光谱法测定植物组织中甜菜碱含量方法的改良[J].广州大学学报(自然科学版),2011,10(3):32-36.GUO P G,SONG B L,XU L G,ZHENG Y S,LI R H,ZHANG H,HUANGH D.An improved spectrophotometric method for determination of betaine content in plants[J].Journal of Guangzhou University(Natural Science Edition),2011,10(3):32-36.
[12]李合生,孙群,赵世杰.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.Li H S,Sun Q,Zhao S J.Principles and techniques of plant physiological biochemical experiments[M].Beijing:Higher Education Press,2000.
[13]叶维杨.厦门地区10种园林植物的耐盐性研究[J].现代农业科学,2008(8):16-18.YE W Y.Study on salt tolerance of 10 species of garden plants in Xiamen area[J].Modern Agricultural Sciences,2008(8):16-18.
[14]左凤月.盐胁迫对3种白刺生长、生理生化及解剖结构的影响[D].重庆:西南大学,2013.ZUO F Y.The effect of NaCl treatment on seed germination,the seedling growth,the leaf physiology and biochemistry and the anatomical structures of leaf and stem of 3 Nitraria species[D].Chong Qing:Southwestern University,2013.
[15]华春,周泉澄,王小平,夏静,陈莉.外源GA3对盐胁迫下北美海蓬子种子萌发及幼苗生长的影响[J].南京师大学报(自然科学版),2007,30(1):82-87.HUA C,ZHOU Q C,WANG X P,XIA J,CHEN L.Effects of Exogenous GA3 on germination and seedling growth of Salicornia Bigelovii Torr.under NaCl stress[J].Journal of Nanjing Normal University(natural science edition),2007,30(1):82-87.
[16]孙海菁,王树凤,陈益泰.盐胁迫对6个树种的生长及生理指标的影响[J].林业科学研究,2009,22(3):315-324.SUN H J,WANG S F,CHEN Y T.Effects of salt stress on growth and physiological index of 6 tree species[J].Forest research,2009,22(3):315-324.
[17]孙聪聪,赵海燕,郑彩霞.NaCl胁迫对银杏幼树渗透调节物质及脯氨酸代谢的影响[J].植物生理学报,2017,53(3):470-476.SUN C C,ZHAO H Y,ZHENG C X.Effects of NaCl stress on osmolyte and proline metabolism in Ginkgo biloba Seedling[J].Plant Physiology Journal,2017,53(3):470-476.
[18]齐延巧,孙静芳,赵世荣,李勇,王建友,王琴,廖康.枸杞幼苗对NaCl和Na2CO3胁迫的生理响应[J].新疆农业科学,2017,54(4):605-617.QI Y Q,SUN J F,ZHAO S R,LI Y,WANG J Y,WANG Q,LIAOK.Physiological response of Lycium bararum L.seedling to NaCl and Na2CO3 stres[J].Xinjiang Agricultural Sciences,2017,54(4):605-617.
[19]朱世杨,刘庆,张小玲,罗天宽,唐征,荆赞革,裘波音,吴海涛,徐谦.盐胁迫对花椰菜苗期幼苗生物量及脯氨酸含量的影响[J].西南农业学报,2016,29(1):148-152.ZHU S Y,LIU Q,ZHANG X L,LUO T K,TANG Z,JING Z G,QIU BY,WU H T,XU Q.Effect of salt stress on biomass and proline content of cauliflower seedlings[J].Southwest China Journal of Agricultural Sciences,2016,29(1):148-152.
[20]龙聪颖,邓辉茗,苏明洁,蔡仕珍,徐小明.3种盐胁迫对蓟幼苗生长及生理生化的影响[J].草业科学,2017,34(12):2484-2492.LONG C Y,DENG H M,SU M J,CAI S Z,Xu X M.Effects of three salt stresses on growth,physiology,and biochemistry of Cirsium japonicum[J].Pratacultural Science,2017,34(12):2484-2492.
[21]赵明德,刘攀,杨冲,闫国苍,王文颖.盐胁迫对青藏高原多年生牧草幼苗生理指标的影响[J].生态科学,2018,37(3):123-130.ZHAO M D,LIU P,YANG C,YAN G C,WANG W Y.Effects of salt stress on physiological indexes of perennial forage seedlings in Qinghai-Tibet Plateau[J].Ecological Science,2018,37(3):123-130.
[22]陈少良,李金克,毕望富,王沙生.盐胁迫条件下杨树盐分与甜菜碱及糖类物质变化[J].植物学通报,2001,18(5):587-596.CHEN S L,LI J K,BI W F,WANG S S.Genotypic variation in accumulation of salt ions,betaine and sugars in poplar under conditions of salt stress[J].Chinese Bulletin of Botany,2001,18(5):587-596.
[2]陈宏毅.冰菜的生物学特性与栽培技术[J].蔬菜,2016(8):42-45.CHEN H Y.Biological characteristics and cultivation techniques of Mesembryanthemum crystallinum L[J].Vegetables,2016(8):42-45.
[3]汪李平.长江流域塑料大棚冰菜栽培技术[J].长江蔬菜,2018(12):21-24.WANG L P.Cultivation Techniques of Mesembryanthemum crystallinum L.in plastic greenhouse in YangtzeRiver Basin[J].Journal of Changjiang Vegetables,2018(12):21-24.
[4]韩明丽,沈卫新,赵根,陈丽萍.保健蔬菜冰菜的生物学特性及关键栽培技术[J].湖南农业科学,2018(2):31-33.HAN M L,SHEN W X,ZHAO G,CHEN L P.The Biological characteristics and key cultivation techniques of healthy vegetable Mesembryanthemum crystallinum L[J].Hunan Agricultural Sciences,2018(2):31-33.
[5]IRENEUSZ S,MARTA L,ZBIGNIEW M.The foliar concentration of hydrogen peroxide during salt-induced Ca-CAM transition in Mesembryanthemum crystallinum L.[J].Plant Science,2008,174(2):221-226.
[6]AGARIE S,SHIMODA T,SHIMIZU Y,BAUMANN K,SUNAGAWA H,KONDO A,Ueno O,NAKAHARA T,NOSE A,CUSHMAN J C.Salt tolerance,salt accumulation,and ionic homeostasis in an epidermal bladder-cell-less mutant of the common ice plant Mesembryanthemum crystallinum[J].Journal of experimental botany,2007,58(8):1957-1967.
[7]徐微风,覃和业,刘姣,王蕾,吕瑞,郭建春,符少萍,李瑞梅,胡新文,段瑞军.冰菜在不同浓度海水胁迫下的氧化胁迫和抗氧化酶活性变化[J].江苏农业学报,2017,33(4):775-781.XU W F,QIN H Y,LIU J,WANG L,LV R,GUO J C,FU S P,LI R M,HUX W,DUAN R J.Changes of oxidative stress and antioxidant enzyme activity of Mesembryanthe-mum crystallinum Linnaeus in response to different concentrations of seawater[J].Jiangsu Journal of Agricultural Sciences,2017,33(4):775-781.
[8]郑炳松.现代植物生理生化研究技术[M].北京:气象出版社,2006.ZHENG B S.Modern Plant Physiological and Biochemical Research Technology[M].Beijing:China Meteorological Press,2006.
[9]陈建勋,王晓峰.植物生理学实验指导[M].第2版.广州:华南理工大学出版社,2006.CHEN J X,WANG X F.Guide to Plant Physiology Experiment[M].2nd Edition.Guangzhou:SouthChina University of Technology Press,2006.
[10]张宪政.作物生理研究法[M].北京:农业出版社,1992:129-150.ZHANG X Z.Crop physiology research method[M].Bei jing:Agricultural Press,1992:129-150.
[11]郭培国,宋波龙,许兰桂,郑岩松,李荣华,张华,黄红娣.光谱法测定植物组织中甜菜碱含量方法的改良[J].广州大学学报(自然科学版),2011,10(3):32-36.GUO P G,SONG B L,XU L G,ZHENG Y S,LI R H,ZHANG H,HUANGH D.An improved spectrophotometric method for determination of betaine content in plants[J].Journal of Guangzhou University(Natural Science Edition),2011,10(3):32-36.
[12]李合生,孙群,赵世杰.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.Li H S,Sun Q,Zhao S J.Principles and techniques of plant physiological biochemical experiments[M].Beijing:Higher Education Press,2000.
[13]叶维杨.厦门地区10种园林植物的耐盐性研究[J].现代农业科学,2008(8):16-18.YE W Y.Study on salt tolerance of 10 species of garden plants in Xiamen area[J].Modern Agricultural Sciences,2008(8):16-18.
[14]左凤月.盐胁迫对3种白刺生长、生理生化及解剖结构的影响[D].重庆:西南大学,2013.ZUO F Y.The effect of NaCl treatment on seed germination,the seedling growth,the leaf physiology and biochemistry and the anatomical structures of leaf and stem of 3 Nitraria species[D].Chong Qing:Southwestern University,2013.
[15]华春,周泉澄,王小平,夏静,陈莉.外源GA3对盐胁迫下北美海蓬子种子萌发及幼苗生长的影响[J].南京师大学报(自然科学版),2007,30(1):82-87.HUA C,ZHOU Q C,WANG X P,XIA J,CHEN L.Effects of Exogenous GA3 on germination and seedling growth of Salicornia Bigelovii Torr.under NaCl stress[J].Journal of Nanjing Normal University(natural science edition),2007,30(1):82-87.
[16]孙海菁,王树凤,陈益泰.盐胁迫对6个树种的生长及生理指标的影响[J].林业科学研究,2009,22(3):315-324.SUN H J,WANG S F,CHEN Y T.Effects of salt stress on growth and physiological index of 6 tree species[J].Forest research,2009,22(3):315-324.
[17]孙聪聪,赵海燕,郑彩霞.NaCl胁迫对银杏幼树渗透调节物质及脯氨酸代谢的影响[J].植物生理学报,2017,53(3):470-476.SUN C C,ZHAO H Y,ZHENG C X.Effects of NaCl stress on osmolyte and proline metabolism in Ginkgo biloba Seedling[J].Plant Physiology Journal,2017,53(3):470-476.
[18]齐延巧,孙静芳,赵世荣,李勇,王建友,王琴,廖康.枸杞幼苗对NaCl和Na2CO3胁迫的生理响应[J].新疆农业科学,2017,54(4):605-617.QI Y Q,SUN J F,ZHAO S R,LI Y,WANG J Y,WANG Q,LIAOK.Physiological response of Lycium bararum L.seedling to NaCl and Na2CO3 stres[J].Xinjiang Agricultural Sciences,2017,54(4):605-617.
[19]朱世杨,刘庆,张小玲,罗天宽,唐征,荆赞革,裘波音,吴海涛,徐谦.盐胁迫对花椰菜苗期幼苗生物量及脯氨酸含量的影响[J].西南农业学报,2016,29(1):148-152.ZHU S Y,LIU Q,ZHANG X L,LUO T K,TANG Z,JING Z G,QIU BY,WU H T,XU Q.Effect of salt stress on biomass and proline content of cauliflower seedlings[J].Southwest China Journal of Agricultural Sciences,2016,29(1):148-152.
[20]龙聪颖,邓辉茗,苏明洁,蔡仕珍,徐小明.3种盐胁迫对蓟幼苗生长及生理生化的影响[J].草业科学,2017,34(12):2484-2492.LONG C Y,DENG H M,SU M J,CAI S Z,Xu X M.Effects of three salt stresses on growth,physiology,and biochemistry of Cirsium japonicum[J].Pratacultural Science,2017,34(12):2484-2492.
[21]赵明德,刘攀,杨冲,闫国苍,王文颖.盐胁迫对青藏高原多年生牧草幼苗生理指标的影响[J].生态科学,2018,37(3):123-130.ZHAO M D,LIU P,YANG C,YAN G C,WANG W Y.Effects of salt stress on physiological indexes of perennial forage seedlings in Qinghai-Tibet Plateau[J].Ecological Science,2018,37(3):123-130.
[22]陈少良,李金克,毕望富,王沙生.盐胁迫条件下杨树盐分与甜菜碱及糖类物质变化[J].植物学通报,2001,18(5):587-596.CHEN S L,LI J K,BI W F,WANG S S.Genotypic variation in accumulation of salt ions,betaine and sugars in poplar under conditions of salt stress[J].Chinese Bulletin of Botany,2001,18(5):587-596.