云斑天牛对不同受害状态下山核桃挥发物成分的触角电位和行为反应
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摘要
为探索能影响云斑天牛Batocera horsfieldi行为反应的山核桃Carya cathayensis挥发物,采用活体植物动态顶空套袋吸附法收集不同受害状态(健康、天牛取食、天牛产卵和天牛钻蛀后)山核桃释放的挥发物,并运用气相色谱-触角电位测量系统(GC-EAD)及Y型嗅觉仪技术分析鉴定出对云斑天牛有电生理及行为反应的挥发物。结果表明:云斑天牛成虫对不同受害状态下山核桃挥发物中蒎烯、萜品烯、壬醛、α-萜品醇、五甲基苯、丙烯酸异辛酯、2,6-二甲基萘及正十六烷8种挥发物均产生电生理反应。嗅觉反应结果显示:萜品烯对云斑天牛雄成虫产生显著的引诱作用(P<0.05);壬醛对云斑天牛雌成虫产生极显著的引诱作用(P<0.01),对其雄成虫产生显著的引诱作用(P<0.05);而蒎烯对云斑天牛雌成虫产生极显著的驱避作用(P<0.01);α-萜品醇则对其雌成虫产生极显著的引诱作用(P<0.01),对其雄成虫产生显著的驱避作用(P<0.05)。研究结果说明:α-萜品醇和壬醛对云斑天牛雌成虫产生较强的引诱作用,而蒎烯对其雌成虫产生较好的驱避作用;萜品烯和壬醛对云斑天牛雄成虫产生较强的引诱作用,而α-萜品醇则对其雄成虫产生较好的驱避作用。图4表1参25
To explore the volatiles from the Carya cathayensis, which affect the behavioral response of the brown mulberry longhorn beetle, Batocera horsfieldi, and to provide a theoretical basis for screening plant-originated attractants or repellents of this insect. In the C. cathayensis of Lin'an Yayuan Village, 3 C. cathayensis trees were vandamly selected from different damaged states [healthy(ck), as well as feeding, oviposition and boring damage by longicorn beetle]. The volatiles were collected by using dynamic headspace adsorption living plants, and those causing electrophysiological and behavioral responses in B. horsfieldi were identified by gas chromatography-electrophysiological antennal detecting system(GC-EAD) and Y-tube olfactometer. Results showed that the B. horsfieldi adults had significant electrophysiological responses to eight volatiles of C.cathayensis volatiles with different damaged states, including(1 R)-(+)-alpha-pinene, g-terpinene, nonanal, α-terpineol, pentamethyl phenyl, 2-ethylhexyl acrylate, 2,6-dimethylnaphthalene and hexadecane. Female and male adults had the strongest electrophysiological responses to nonanal and α-terpineol followed. The olfactory reaction showed that g-terpinene had a significant attractivity to male adults of B. horsfieldi(P<0.05). Also nonanal had an highly significant attractivity to female adults(P<0.01) and a significant attractivity to male adults(P<0.05). Meanwhlie,(1 R)-(+)-alpha-pinene had an highly significant repellent activity to female adults(P<0.01). In addition α-terpineol had a highly significant attractivity to female adults(P<0.01), but a significant repellent activity to male adults(P <0.05). These results indicate that α-terpineol and nonanal showed strong attractivity to female adults of B. horsfieldi, while(1 R)-(+)-alpha-pinene showed good repellent activity, g-terpinene and nonanal showed strong attractivity to male adults, while α-terpineol showed good repellent activity. [Ch, 4 fig. 1 tab. 25 ref.]
To explore the volatiles from the Carya cathayensis, which affect the behavioral response of the brown mulberry longhorn beetle, Batocera horsfieldi, and to provide a theoretical basis for screening plant-originated attractants or repellents of this insect. In the C. cathayensis of Lin'an Yayuan Village, 3 C. cathayensis trees were vandamly selected from different damaged states [healthy(ck), as well as feeding, oviposition and boring damage by longicorn beetle]. The volatiles were collected by using dynamic headspace adsorption living plants, and those causing electrophysiological and behavioral responses in B. horsfieldi were identified by gas chromatography-electrophysiological antennal detecting system(GC-EAD) and Y-tube olfactometer. Results showed that the B. horsfieldi adults had significant electrophysiological responses to eight volatiles of C.cathayensis volatiles with different damaged states, including(1 R)-(+)-alpha-pinene, g-terpinene, nonanal, α-terpineol, pentamethyl phenyl, 2-ethylhexyl acrylate, 2,6-dimethylnaphthalene and hexadecane. Female and male adults had the strongest electrophysiological responses to nonanal and α-terpineol followed. The olfactory reaction showed that g-terpinene had a significant attractivity to male adults of B. horsfieldi(P<0.05). Also nonanal had an highly significant attractivity to female adults(P<0.01) and a significant attractivity to male adults(P<0.05). Meanwhlie,(1 R)-(+)-alpha-pinene had an highly significant repellent activity to female adults(P<0.01). In addition α-terpineol had a highly significant attractivity to female adults(P<0.01), but a significant repellent activity to male adults(P <0.05). These results indicate that α-terpineol and nonanal showed strong attractivity to female adults of B. horsfieldi, while(1 R)-(+)-alpha-pinene showed good repellent activity, g-terpinene and nonanal showed strong attractivity to male adults, while α-terpineol showed good repellent activity. [Ch, 4 fig. 1 tab. 25 ref.]
引文
[1]严敖金,嵇保中,钱范俊,等.云斑天牛Batocera horsfieldi(Hope)的研究[J].南京林业大学学报(自然科学版),1997, 21(1):1-6.YAN Aojin, JI Baozhong, QIAN Fanjun, et al. A study on Batocera horsfieldi(Hope)[J]. J Nanjing For Univ Nat Sci Ed, 1997, 21(1):1-6.
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[3]朱宁,张冬勇,沈红霞,等.光肩星天牛产卵偏好性及寄生植物挥发物成分分析[J].浙江农林大学学报, 2017, 34(6):1059-1064.ZHU Ning, ZHANG Dongyong, SHEN Hongxia, et al. Oviposition preferences of Anoplophora glabripennis on three host plants and composition analysis of host plant volatiles[J]. J Zhejiang A&F Univ, 2017, 34(6):1059-1064.
[4]STANJEK V, HERHAUS C, RITGEN U, et al. Changes in the leaf surface chemistry of Apium graveolens(Apiaceae)stimulated by jasmonic acid and perceived by a specialist insect[J]. Helvetica Chimica Acta, 1997, 80(5):1408-1420.
[5]RUTHER J, REINECKE A, THIEMANN K, et al. Mate finding in the forest cockchafer, Melolontha hippocastani, mediated by volatiles from plants and females[J]. Physiol Entomol, 2000, 25(2):172-179.
[6]INUI Y, MIYAMOTO Y, OHGUSHI T. Comparison of volatile leaf compounds and herbivorous insect communities on three willow species[J]. Popul Ecol, 2003, 45(1):41-46.
[7]van der GOES, van NATERS W, CARLSON J R. Insects as chemosensors of humans and crops[J]. Nature, 2006,444(7117):302-307.
[8]HU Jiafu, ANGELI S, SCHUETZ S, et al. Ecology and management of exotic and endemic Asian longhorned beetle Anoplophora glabripennis[J]. Agric For Entomol, 2009, 11(4):359-375.
[9]PAR魪P W, TUMLINSON J H. Plant volatiles as a defense against insect herbivores[J]. Plant Physiol, 1999, 121(2):325-331.
[10]ALLISON J D, BORDEN J H, SEYBOLD S J. A review of the chemical ecology of the Cerambycidae(Coleoptera)[J]. Chemoecology, 2004, 14(3/4):123-150.
[11]严善春,张丹丹,迟德福.植物挥发性物质对昆虫作用的研究进展[J].应用生态学报, 2003, 14(2):310-313.YAN Shanchun, ZHANG Dandan, CHI Defu. Advances in studies on the effects of plant volatiles on insects behavior[J]. Chin J Appl Ecol, 2003, 14(2):310-313.
[12]王红伟,杨伟,杨桦,等.横坑切梢小蠹对植物挥发物的电生理和行为反应[J].生态学杂志, 2014, 33(5):1260-1266.WANG Hongwei, YANG Wei, YANG Hua, et al. Electrophysiologram and behavioral responses of Tomicus minor(Hartig)(Coleoptera:Scolytidae)to plant volatiles[J]. Chin J Ecol, 2014, 33(5):1260-1266.
[13]ZHUGE P P, LUO S L, WANG M Q, et al. Electrophysiological responses of Batocera horsfieldi(Hope)adults to plant volatiles[J]. J Appl Entomol, 2010, 134(7):600-607.
[14]张冬勇.山核桃天牛危害调查及其云斑天牛植物源引诱物质研究[D].杭州:浙江农林大学, 2016.ZHANG Dongyong. The Damage Investigation of Carya cathayensis Longhorned Beetles and Study on Plant Attractant of Batocera horsfieldi[D]. Hangzhou:Zhejiang A&F University, 2016.
[15]M譈LLER C, HILKER M. The efffect of a green leaf volatile on host plant finding by larvae of herbivorous insect[J]. Naturwissenschafen, 2000, 87(5):216-219.
[16]杨桦,杨伟,杨茂发,等.法国冬青和光皮桦挥发物日节律及云斑天牛的触角电位反应[J].应用生态学报,2011, 22(2):357-363.YANG Hua, YANG Wei, YANG Maofa, et al. Diurnal rhythm of viburnum awabuki and Betula luminifera volatiles and electroantennogram response of Batocera horsfieldi[J]. Chin J Appl Ecol, 2011, 22(2):357-363.
[17]李继泉,樊慧,金幼菊.光肩星天牛对损伤后复叶槭植株的行为反应[J].北京林业大学学报, 2003, 25(5):42-46.LI Jiquan, FAN Hui, JIN Youju. Behavior respone of Anoplophora glabripennis(Motsh.)to the mechanical-wounded and herbivore-fed Acer negundo L.[J]. J Beijing For Univ, 2003, 25(5):42-46.
[18]马艳,史黎央,赵艺,等.不同危害状态下寄主山核桃挥发物成分的比较及桑天牛对其组分的GC-EAD和行为反应[J].昆虫学报, 2018, 61(5):574-584.MA Yan, SHI Liyang, ZHAO Yi, et al. Comparison of volatiles released from the host Juglans mandshurica in different damaged states and the GC-EAD and behavioral responses of Apriona germari(Coleoptera:Cerambycidae)to these volatiles[J]. Acta Entomol Sin, 2018, 61(5):574-584.
[19]毕拥国,郭丽,王志刚,等.桑天牛对5种植物源挥发性化合物及其混合物的触角电位和行为反应[J].蚕业科学, 2017, 43(1):32-38.BI Yongguo, GUO Li, WANG Zhigang, et al. Electroantennogram and behavioral responses of Apriona germari to five plant volatiles and their mixtures[J]. Sci Seric, 2017, 43(1):32-38.
[20]HERN A, DORN S. A female-specific attractant for the codling moth, Cydia pomonella, from apple fruit volatiles[J]. Naturwissenschaften, 2004, 91(2):77-80.
[21]TOOKER J H, CRUMRIN A L, HANKS L M. Plant volatiles are behavioralcues for adult females of the gall wasp Antistrophus rufus[J]. Chemoecology, 2005, 15(2):85-88.
[22]YANG Hua, YANG Wei, YANG Maofa, et al. Effects of plant volatiles on the EAG and behavioral responses of Batocera horsfieldi Hope(Coleopter:Cerambycidae)[J]. J Agric Urban Entomol, 2010, 27(1):20-32.
[23]YANG Hua, YANG Wei, YANG Maofa, et al. The EAG and behavioral responses of Batocera horsfieldi(Coleoptera:Cerambycidae)to the composition of volatiles[J]. Kansas Entomol Soc, 2011, 84(3):217-231.
[24]BARATA E N, MUSTAPARTA H, PICKENTT J A, et al. Encoding of host and non-host plant odours by receptor neurons in the eucalyptus woodborer, Phoracantha semipunctata(Coleoptera:Cerambycidae)[J]. J Comp Physiol,2002, 188(2):121-133.
[25]PICHERSKY E, GERSHENZON J. The formation and function of plant volatiles:perfumes for pollinator attraction and defens[J]. Curr Opin Plant Biol, 2002, 5(3):237-243.
[2]张冬勇,周书永,朱宁,等.山核桃天牛危害调查及种类的鉴别方法[J].浙江农林大学学报, 2017, 34(1):42-49.ZHANG Dongyong, ZHOU Shuyong, ZHU Ning, et al. Damage investigation and an identification method for different species of Carya cathayensis longhorned beetles[J]. J Zhejiang A&F Univ, 2017, 34(1):42-49.
[3]朱宁,张冬勇,沈红霞,等.光肩星天牛产卵偏好性及寄生植物挥发物成分分析[J].浙江农林大学学报, 2017, 34(6):1059-1064.ZHU Ning, ZHANG Dongyong, SHEN Hongxia, et al. Oviposition preferences of Anoplophora glabripennis on three host plants and composition analysis of host plant volatiles[J]. J Zhejiang A&F Univ, 2017, 34(6):1059-1064.
[4]STANJEK V, HERHAUS C, RITGEN U, et al. Changes in the leaf surface chemistry of Apium graveolens(Apiaceae)stimulated by jasmonic acid and perceived by a specialist insect[J]. Helvetica Chimica Acta, 1997, 80(5):1408-1420.
[5]RUTHER J, REINECKE A, THIEMANN K, et al. Mate finding in the forest cockchafer, Melolontha hippocastani, mediated by volatiles from plants and females[J]. Physiol Entomol, 2000, 25(2):172-179.
[6]INUI Y, MIYAMOTO Y, OHGUSHI T. Comparison of volatile leaf compounds and herbivorous insect communities on three willow species[J]. Popul Ecol, 2003, 45(1):41-46.
[7]van der GOES, van NATERS W, CARLSON J R. Insects as chemosensors of humans and crops[J]. Nature, 2006,444(7117):302-307.
[8]HU Jiafu, ANGELI S, SCHUETZ S, et al. Ecology and management of exotic and endemic Asian longhorned beetle Anoplophora glabripennis[J]. Agric For Entomol, 2009, 11(4):359-375.
[9]PAR魪P W, TUMLINSON J H. Plant volatiles as a defense against insect herbivores[J]. Plant Physiol, 1999, 121(2):325-331.
[10]ALLISON J D, BORDEN J H, SEYBOLD S J. A review of the chemical ecology of the Cerambycidae(Coleoptera)[J]. Chemoecology, 2004, 14(3/4):123-150.
[11]严善春,张丹丹,迟德福.植物挥发性物质对昆虫作用的研究进展[J].应用生态学报, 2003, 14(2):310-313.YAN Shanchun, ZHANG Dandan, CHI Defu. Advances in studies on the effects of plant volatiles on insects behavior[J]. Chin J Appl Ecol, 2003, 14(2):310-313.
[12]王红伟,杨伟,杨桦,等.横坑切梢小蠹对植物挥发物的电生理和行为反应[J].生态学杂志, 2014, 33(5):1260-1266.WANG Hongwei, YANG Wei, YANG Hua, et al. Electrophysiologram and behavioral responses of Tomicus minor(Hartig)(Coleoptera:Scolytidae)to plant volatiles[J]. Chin J Ecol, 2014, 33(5):1260-1266.
[13]ZHUGE P P, LUO S L, WANG M Q, et al. Electrophysiological responses of Batocera horsfieldi(Hope)adults to plant volatiles[J]. J Appl Entomol, 2010, 134(7):600-607.
[14]张冬勇.山核桃天牛危害调查及其云斑天牛植物源引诱物质研究[D].杭州:浙江农林大学, 2016.ZHANG Dongyong. The Damage Investigation of Carya cathayensis Longhorned Beetles and Study on Plant Attractant of Batocera horsfieldi[D]. Hangzhou:Zhejiang A&F University, 2016.
[15]M譈LLER C, HILKER M. The efffect of a green leaf volatile on host plant finding by larvae of herbivorous insect[J]. Naturwissenschafen, 2000, 87(5):216-219.
[16]杨桦,杨伟,杨茂发,等.法国冬青和光皮桦挥发物日节律及云斑天牛的触角电位反应[J].应用生态学报,2011, 22(2):357-363.YANG Hua, YANG Wei, YANG Maofa, et al. Diurnal rhythm of viburnum awabuki and Betula luminifera volatiles and electroantennogram response of Batocera horsfieldi[J]. Chin J Appl Ecol, 2011, 22(2):357-363.
[17]李继泉,樊慧,金幼菊.光肩星天牛对损伤后复叶槭植株的行为反应[J].北京林业大学学报, 2003, 25(5):42-46.LI Jiquan, FAN Hui, JIN Youju. Behavior respone of Anoplophora glabripennis(Motsh.)to the mechanical-wounded and herbivore-fed Acer negundo L.[J]. J Beijing For Univ, 2003, 25(5):42-46.
[18]马艳,史黎央,赵艺,等.不同危害状态下寄主山核桃挥发物成分的比较及桑天牛对其组分的GC-EAD和行为反应[J].昆虫学报, 2018, 61(5):574-584.MA Yan, SHI Liyang, ZHAO Yi, et al. Comparison of volatiles released from the host Juglans mandshurica in different damaged states and the GC-EAD and behavioral responses of Apriona germari(Coleoptera:Cerambycidae)to these volatiles[J]. Acta Entomol Sin, 2018, 61(5):574-584.
[19]毕拥国,郭丽,王志刚,等.桑天牛对5种植物源挥发性化合物及其混合物的触角电位和行为反应[J].蚕业科学, 2017, 43(1):32-38.BI Yongguo, GUO Li, WANG Zhigang, et al. Electroantennogram and behavioral responses of Apriona germari to five plant volatiles and their mixtures[J]. Sci Seric, 2017, 43(1):32-38.
[20]HERN A, DORN S. A female-specific attractant for the codling moth, Cydia pomonella, from apple fruit volatiles[J]. Naturwissenschaften, 2004, 91(2):77-80.
[21]TOOKER J H, CRUMRIN A L, HANKS L M. Plant volatiles are behavioralcues for adult females of the gall wasp Antistrophus rufus[J]. Chemoecology, 2005, 15(2):85-88.
[22]YANG Hua, YANG Wei, YANG Maofa, et al. Effects of plant volatiles on the EAG and behavioral responses of Batocera horsfieldi Hope(Coleopter:Cerambycidae)[J]. J Agric Urban Entomol, 2010, 27(1):20-32.
[23]YANG Hua, YANG Wei, YANG Maofa, et al. The EAG and behavioral responses of Batocera horsfieldi(Coleoptera:Cerambycidae)to the composition of volatiles[J]. Kansas Entomol Soc, 2011, 84(3):217-231.
[24]BARATA E N, MUSTAPARTA H, PICKENTT J A, et al. Encoding of host and non-host plant odours by receptor neurons in the eucalyptus woodborer, Phoracantha semipunctata(Coleoptera:Cerambycidae)[J]. J Comp Physiol,2002, 188(2):121-133.
[25]PICHERSKY E, GERSHENZON J. The formation and function of plant volatiles:perfumes for pollinator attraction and defens[J]. Curr Opin Plant Biol, 2002, 5(3):237-243.