子宫动脉介入化疗联合栓塞治疗前后宫颈癌组织miR-143和岩藻糖基转移酶Ⅳ表达及意义
|
摘要
目的探讨宫颈癌患者行子宫动脉介入化疗联合栓塞治疗前、后宫颈癌组织miR-143和岩藻糖基转移酶4(fucosyltransferase 4,FUT4)表达变化及意义。方法 20例宫颈癌患者,采用多西他赛+顺铂方案子宫动脉介入化疗联合栓塞治疗3周后行手术治疗,采用实时荧光定量PCR法检测化疗前、后宫颈癌组织中miR-143表达,采用Western blot法检测FUT4蛋白表达,Pearson线性相关分析宫颈癌组织miR-143与FUT4表达的相关性。结果化疗后宫颈癌组织miR-143相对表达量(2.11±0.17)较化疗前(1.04±0.10)增高,FUT4相对表达量(0.38±0.08)较化疗前(0.93±0.09)降低(P<0.05);miR-143表达在化疗前、后与FUT4均呈负相关(r=-0.509,P=0.020;r=-0.563,P=0.010)。结论多西他赛+顺铂方案子宫动脉介入化疗联合栓塞治疗可上调miR-143及下调FUT4在宫颈癌组织中的表达。
Objective To investigate the expressions of miR-143 and fucosyltransferase 4(FUT4)in cervical cancer before and after uterine arterial chemotherapy combined with embolization.Methods Twenty patients with cervical cancer were performed operation after 3-week treatment with docetaxel+ cisplatin regimen uterine artery interventional chemotherapy combined with embolization.The expression of miR-143 in cervical cancer tissue before and after treatment was detected by real-time quantitative PCR and the expression of FUT4 protein was detected by Western blot.The correlation between miR-143 and FUT4 was analyzed by Pearson linear correlation method.Results The relative expression of miR-143 was significantly higher after treatment(2.11±0.17)than that before treatment(1.04±0.10),and the relative expression of FUT4 was significantly lower after treatment(0.38±0.08)than that before treatment(0.93±0.09)(P<0.05).MiR-143 was negatively correlated with FUT4 both before and after treatment(r=-0.509,P=0.020;r=-0.563,P=0.010).Conclusion Docetaxel+cisplatin regimen can up-regulate miR-143 expression and down-regulate FUT4 expression in cervical cancer.
Objective To investigate the expressions of miR-143 and fucosyltransferase 4(FUT4)in cervical cancer before and after uterine arterial chemotherapy combined with embolization.Methods Twenty patients with cervical cancer were performed operation after 3-week treatment with docetaxel+ cisplatin regimen uterine artery interventional chemotherapy combined with embolization.The expression of miR-143 in cervical cancer tissue before and after treatment was detected by real-time quantitative PCR and the expression of FUT4 protein was detected by Western blot.The correlation between miR-143 and FUT4 was analyzed by Pearson linear correlation method.Results The relative expression of miR-143 was significantly higher after treatment(2.11±0.17)than that before treatment(1.04±0.10),and the relative expression of FUT4 was significantly lower after treatment(0.38±0.08)than that before treatment(0.93±0.09)(P<0.05).MiR-143 was negatively correlated with FUT4 both before and after treatment(r=-0.509,P=0.020;r=-0.563,P=0.010).Conclusion Docetaxel+cisplatin regimen can up-regulate miR-143 expression and down-regulate FUT4 expression in cervical cancer.
引文
[1]Zhou J,Wu SG,Sun JY,et al.Comparison of clinical outcomes of squamous cell carcinoma,adenocarcinoma,and adenosquamous carcinoma of the uterine cervix after definitive radiotherapy:apopulation-based analysis[J].J Cancer Res Clin Oncol,2017,143(1):115-122.
[2]Shi JF,Canfell K,Lew JB,et al.The burden of cervical cancer in China:synthesis of the evidence[J].Int J Cancer,2012,130(3):641-652.
[3]严冬梅.宫颈癌术前新辅助化疗47例疗效观察[J].中华实用诊断与治疗杂志,2010,24(2):192-194.
[4]Mccormack M,Kadalayil L,Hackshaw A,et al.A phaseⅡstudy of weekly neoadjuvant chemotherapy followed by radical chemoradiation for locally advanced cervical cancer[J].Br J Cancer,2013,108(12):2464-2469.
[5]李连香,陈丽宏.宫颈癌新辅助化疗miRNA的差异表达[J].现代中西医结合杂志,2014,23(2):122-124.
[6]林仲秋.《FIGO 2015妇癌报告》解读连载一:宫颈癌诊治指南解读[J].中国实用妇科与产科杂志,2015,31(11):981-985.
[7]张蔚,刘珍,胡晓霞,等.宫颈癌中异常表达microRNA与HPV-16E6/E7基因相关性分析[J].中华实用诊断与治疗杂志,2016,30(2):120-123.
[8]Chen Y,Ma C,Zhang W,et al.Down regulation of miR-143is related with tumor size,lymph node metastasis and HPV16infection in cervical squamous cancer[J].Diagn Pathol,2014,9:88.
[9]Zheng F,Zhang J,Luo S,et al.MiR-143is associated with proliferation and apoptosis involving ERK5in HeLa cells[J].Oncol Lett,2016,12(4):3021-3027.
[10]Arai Y,Nishida M.Differential diagnosis between normal endometrium and endometrial hyperplasia with immunostaining cytology using anti-LeY monoclonal antibody[J].Int J Gynecol Cancer,2003,13(1):42-46.
[11]Wang A,Chang L,Zhen N,et al.Tumor-associated macrophages promote Ezrin phosphorylation-mediated epithelial-mesenchymal transition in lung adenocarcinoma through FUT4/LeY up-regulation[J].Oncotarget,2017,8(17):28247-28259.
[12]朱雨彤,佟少明,张叶军,等.鳞癌细胞增殖能力与岩藻糖基转移酶表达水平及糖蛋白岩藻糖基化修饰有关[J].中国生物化学与分子生物学报,2016,32(7):830-836.
[13]Tian L,Shen D,Li X,et al.Ginsenoside Rg3 inhibits epithelial-mesenchymal transition(EMT)and invasion of lung cancer by down-regulating FUT4[J].Oncotarget,2016,7(2):1619-1632.
[14]Yang X,Liu S,Yan Q.Role of fucosyltransferaseⅣin epithelial-mesenchymal transition in breast cancer cells[J].Cell Death Dis,2013,4(7):e735.
[15]Li Y,Sun Z,Liu B,et al.Tumor-suppressive miR-26aand miR-26binhibit cell aggressiveness by regulating FUT4in colorectal cancer[J].Cell Death Dis,2017,8(6):e2892.
[16]Yang X,Zhang Z,Jia S,et al.Overexpression of fucosyltransferaseⅣin A431 cell line increases cell proliferation[J].Int J Biochem Cell Biol,2007,39(9):1722-1730.
[2]Shi JF,Canfell K,Lew JB,et al.The burden of cervical cancer in China:synthesis of the evidence[J].Int J Cancer,2012,130(3):641-652.
[3]严冬梅.宫颈癌术前新辅助化疗47例疗效观察[J].中华实用诊断与治疗杂志,2010,24(2):192-194.
[4]Mccormack M,Kadalayil L,Hackshaw A,et al.A phaseⅡstudy of weekly neoadjuvant chemotherapy followed by radical chemoradiation for locally advanced cervical cancer[J].Br J Cancer,2013,108(12):2464-2469.
[5]李连香,陈丽宏.宫颈癌新辅助化疗miRNA的差异表达[J].现代中西医结合杂志,2014,23(2):122-124.
[6]林仲秋.《FIGO 2015妇癌报告》解读连载一:宫颈癌诊治指南解读[J].中国实用妇科与产科杂志,2015,31(11):981-985.
[7]张蔚,刘珍,胡晓霞,等.宫颈癌中异常表达microRNA与HPV-16E6/E7基因相关性分析[J].中华实用诊断与治疗杂志,2016,30(2):120-123.
[8]Chen Y,Ma C,Zhang W,et al.Down regulation of miR-143is related with tumor size,lymph node metastasis and HPV16infection in cervical squamous cancer[J].Diagn Pathol,2014,9:88.
[9]Zheng F,Zhang J,Luo S,et al.MiR-143is associated with proliferation and apoptosis involving ERK5in HeLa cells[J].Oncol Lett,2016,12(4):3021-3027.
[10]Arai Y,Nishida M.Differential diagnosis between normal endometrium and endometrial hyperplasia with immunostaining cytology using anti-LeY monoclonal antibody[J].Int J Gynecol Cancer,2003,13(1):42-46.
[11]Wang A,Chang L,Zhen N,et al.Tumor-associated macrophages promote Ezrin phosphorylation-mediated epithelial-mesenchymal transition in lung adenocarcinoma through FUT4/LeY up-regulation[J].Oncotarget,2017,8(17):28247-28259.
[12]朱雨彤,佟少明,张叶军,等.鳞癌细胞增殖能力与岩藻糖基转移酶表达水平及糖蛋白岩藻糖基化修饰有关[J].中国生物化学与分子生物学报,2016,32(7):830-836.
[13]Tian L,Shen D,Li X,et al.Ginsenoside Rg3 inhibits epithelial-mesenchymal transition(EMT)and invasion of lung cancer by down-regulating FUT4[J].Oncotarget,2016,7(2):1619-1632.
[14]Yang X,Liu S,Yan Q.Role of fucosyltransferaseⅣin epithelial-mesenchymal transition in breast cancer cells[J].Cell Death Dis,2013,4(7):e735.
[15]Li Y,Sun Z,Liu B,et al.Tumor-suppressive miR-26aand miR-26binhibit cell aggressiveness by regulating FUT4in colorectal cancer[J].Cell Death Dis,2017,8(6):e2892.
[16]Yang X,Zhang Z,Jia S,et al.Overexpression of fucosyltransferaseⅣin A431 cell line increases cell proliferation[J].Int J Biochem Cell Biol,2007,39(9):1722-1730.