β-蜕皮甾酮对激素性骨质疏松大鼠血清骨代谢指标及骨密度的影响
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摘要
目的:观察β-蜕皮甾酮(β-Ecd)对糖皮质激素性骨质疏松大鼠血清骨代谢指标及骨密度(BMD)的影响。方法:30只雄性Wistar大鼠随机分为5组:对照组、泼尼松龙(Pred)组、Pred+β-Ecd(5 mg/kg)组、Pred+β-Ecd(10 mg/kg)组、Pred+阿仑膦酸(ALN)(3 mg/kg)组,每组6只。采用全自动生化分析仪检测各组大鼠血清钙、磷浓度、抗酒石酸磷酸酶(TRAP)和碱性磷酸酶(ALP)活性。应用Micro-CT测定各组大鼠第5 腰椎骨结构和BMD。结果:血清骨代谢指标检测结果显示,与对照组比较Pred组血清钙、磷水平显著升高,血清ALP活性和TRAP活性升高。与Pred组比,Pred+ALN组和Pred+β-Ecd(10 mg/kg)组血清钙、磷水平降低,血清ALP活性和TRAP活性降低。而Pred+β-Ecd(5 mg/kg)组血清ALP活性和TRAP水平降低,而对钙、磷水平无明显影响。骨结构和BMD Micro-CT检测结果显示,在所有Pred处理的大鼠中均观察到不同程度的骨丢失。在Pred组中第5腰椎的BMD值明显低于对照组。Micro-CT评估显示Pred组骨表面积和组织体积的比值(BS/TV)较对照组降低。三维模型分析显示,Pred组骨小梁数量(Tb.N)、骨体积分数(BV/TV)均明显低于对照组,并且骨小梁分离度(Tb.Sp)明显增加,但骨小梁厚度(Tb.Th)和结构模型指数(SMI)未表现出显著差异。与Pred组比,Pred+ALN组和Pred+β-Ecd(10 mg/kg)组BMD、BS/TV、BV/TV和Tb.N水平显著增加,而Tb.Sp和SMI降低。与Pred组相比,Pred+β-Ecd(5 mg/kg)组中BMD、BS/TV、BV/TV和Tb.N水平显著增高,而Tb.Th和Tb.Sp水平降低。结论:β-Ecd可能通过抑制骨丢失、改善骨代谢的作用机制,治疗糖皮质激素性骨质疏松症。
Objective: To observe the effects of β-Ecdysterone(β-Ecd) on serum biochemical indexes and bone mineral density(BMD) in rats with glucocorticoid-induced osteoporosis. Methods: A total of 30 male Wistar rats were randomly divided into five groups: control group, prednisolone(Pred) group, Pred+β-Ecd(5 mg/kg) group, Pred+β-Ecd(10 mg/kg) group and Pred+alendronate(ALN) group(3 mg/kg) with 6 rats in each group. The concentration of calcium and phosphorus, the activities of antitartrate phosphatase(TRAP) and alkaline phosphatase(ALP) in serum of rats in each group were determined by automatic biochemistry analyzer. An analysis of bone structure and BMD of the fifth lumber vertebra of rats in each group was made by Micro-CT. Results: Compared with the control group, the serum calcium and phosphorus levels in Pred group significantly increased and the serum ALP activity and TRAP activity increased. Compared with Pred group, Pred+ALN group and Pred+β-Ecd(10 mg/kg) group had lower serum calcium and phosphorus levels, lower serum ALP activity and lower TRAP activity. In Pred+β-Ecd(5 mg/kg) group, the activity of ALP and the level of TRAP decreased, but the levels of calcium and phosphorus were not significantly affected. Bone structure and BMD Micro-CT results showed that different degree of bone loss was observed in all Pred treated rats. In the Pred group, the BMD value of the fifth lumbar vertebra was significantly lower than that of the control group. Micro-CT evaluation showed that the ratio of bone surface area to tissue volume(BS/TV) in Pred group was lower than that in control group. Three-dimensional model analysis showed that the number of trabecular bone(Tb.N) and bone volume fraction(BV/TV) in prednisolone group were significantly lower than those in control group. The trabecular separation degree(Tb.Sp) was significantly increased in prednisolone group. However, there was no significant difference in bone trabecular thickness(Tb.Th) and structural model index(SMI). Compared with Pred group, BMD, BS/TV, BV/TV and Tb.N levels in Pred+ALN group and Pred+β-Ecd(10 mg/kg) group significantly increased, but Tb.Sp and SMI levels decreased. Compared with Pred group, the levels of BMD, BS/TV, BV/TV and Tb.N in Pred+β-Ecd(5 mg/kg) group were significantly higher, but Tb.Th and Tb.Sp levels were lower. Conclusion: β-Ecd may be applied to the treatment of glucocorticoid osteoporosis by prohibiting bone loss and improving bone metabolism.
Objective: To observe the effects of β-Ecdysterone(β-Ecd) on serum biochemical indexes and bone mineral density(BMD) in rats with glucocorticoid-induced osteoporosis. Methods: A total of 30 male Wistar rats were randomly divided into five groups: control group, prednisolone(Pred) group, Pred+β-Ecd(5 mg/kg) group, Pred+β-Ecd(10 mg/kg) group and Pred+alendronate(ALN) group(3 mg/kg) with 6 rats in each group. The concentration of calcium and phosphorus, the activities of antitartrate phosphatase(TRAP) and alkaline phosphatase(ALP) in serum of rats in each group were determined by automatic biochemistry analyzer. An analysis of bone structure and BMD of the fifth lumber vertebra of rats in each group was made by Micro-CT. Results: Compared with the control group, the serum calcium and phosphorus levels in Pred group significantly increased and the serum ALP activity and TRAP activity increased. Compared with Pred group, Pred+ALN group and Pred+β-Ecd(10 mg/kg) group had lower serum calcium and phosphorus levels, lower serum ALP activity and lower TRAP activity. In Pred+β-Ecd(5 mg/kg) group, the activity of ALP and the level of TRAP decreased, but the levels of calcium and phosphorus were not significantly affected. Bone structure and BMD Micro-CT results showed that different degree of bone loss was observed in all Pred treated rats. In the Pred group, the BMD value of the fifth lumbar vertebra was significantly lower than that of the control group. Micro-CT evaluation showed that the ratio of bone surface area to tissue volume(BS/TV) in Pred group was lower than that in control group. Three-dimensional model analysis showed that the number of trabecular bone(Tb.N) and bone volume fraction(BV/TV) in prednisolone group were significantly lower than those in control group. The trabecular separation degree(Tb.Sp) was significantly increased in prednisolone group. However, there was no significant difference in bone trabecular thickness(Tb.Th) and structural model index(SMI). Compared with Pred group, BMD, BS/TV, BV/TV and Tb.N levels in Pred+ALN group and Pred+β-Ecd(10 mg/kg) group significantly increased, but Tb.Sp and SMI levels decreased. Compared with Pred group, the levels of BMD, BS/TV, BV/TV and Tb.N in Pred+β-Ecd(5 mg/kg) group were significantly higher, but Tb.Th and Tb.Sp levels were lower. Conclusion: β-Ecd may be applied to the treatment of glucocorticoid osteoporosis by prohibiting bone loss and improving bone metabolism.
引文
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[2]梁伟,李理,李兵.降钙素基因相关肽对骨质疏松大鼠骨髓基质干细胞增殖及分化的影响[J].中华实验外科杂志,2018, 35(1):41-45.
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[5]田丽花,梁敏,张劼,等.糖皮质激素性骨质疏松大鼠模型建立的研究[J].广西医科大学学报, 2013, 30(1):5-7.
[6]HUANG Y, BO Y, WU X, et al. An intergated serum and urinary metabonomic research based on UPLC-MS and therapeutic effects of Gushudan on prednisolone-induced osteoporosis rats[J]. J Chromatogr B, 2016, 1027(5):119-130.
[7]TANG Y H, YUE Z S, XIN D W, et al.βEcdysterone promote autophagy and inhibits apoptosis in osteoporotic rats[J]. Mol Med Rep, 2018, 17(1):1591-1598.
[8]孙晓晖,陈文双,陈春玲,等.长期使用糖皮质激素对大鼠的骨生物力学影响[J].中国骨质疏松杂志, 2015, 21(4):418-420.
[9]孙强,刘建.大豆苷元对糖皮质激素性骨质疏松大鼠骨量及骨微结构的影响[J].海南医学院学报, 2013, 19(6):731-734.
[10]毛传万,郑文龙,虞志康,等.温州市791例健康成人腰椎和髋部骨密度测量分析[J].温州医科大学学报, 2016,46(5):369-371.
[11]ROUX J P, WEGRZYN J, BOUTROY S, et al. The predictive value of trabecular bone score(TBS)on whole lumbar vertebrae mechanics:An ex vivo study[J]. Osteoporos Int,2013, 24(9):2455-2460.
[12]JEHLE S, HULTER H N, KRAPF R. Effect of potassium citrate on bone density microarchitecture and fracture risk in healthy older adults without osteoporosis:A randomized controlled trial[J]. J Clin Endocrinol Metab, 2013, 98(1):207-217.
[2]梁伟,李理,李兵.降钙素基因相关肽对骨质疏松大鼠骨髓基质干细胞增殖及分化的影响[J].中华实验外科杂志,2018, 35(1):41-45.
[3]朱辉,郑洪新,林庶如,等.补肾中药对糖皮质激素性骨质疏松模型大鼠的干预作用[J].世界中医中药杂志, 2012,34(2):134-136.
[4]TANG Y H, YUE Z S, LI G S, et al. Effect ofβ-Ecdysterone onglucocorticoid-inducedapoptosisandautophagyinosteoblast[J]. Mol Med Rep, 2018, 17(1):158-164.
[5]田丽花,梁敏,张劼,等.糖皮质激素性骨质疏松大鼠模型建立的研究[J].广西医科大学学报, 2013, 30(1):5-7.
[6]HUANG Y, BO Y, WU X, et al. An intergated serum and urinary metabonomic research based on UPLC-MS and therapeutic effects of Gushudan on prednisolone-induced osteoporosis rats[J]. J Chromatogr B, 2016, 1027(5):119-130.
[7]TANG Y H, YUE Z S, XIN D W, et al.βEcdysterone promote autophagy and inhibits apoptosis in osteoporotic rats[J]. Mol Med Rep, 2018, 17(1):1591-1598.
[8]孙晓晖,陈文双,陈春玲,等.长期使用糖皮质激素对大鼠的骨生物力学影响[J].中国骨质疏松杂志, 2015, 21(4):418-420.
[9]孙强,刘建.大豆苷元对糖皮质激素性骨质疏松大鼠骨量及骨微结构的影响[J].海南医学院学报, 2013, 19(6):731-734.
[10]毛传万,郑文龙,虞志康,等.温州市791例健康成人腰椎和髋部骨密度测量分析[J].温州医科大学学报, 2016,46(5):369-371.
[11]ROUX J P, WEGRZYN J, BOUTROY S, et al. The predictive value of trabecular bone score(TBS)on whole lumbar vertebrae mechanics:An ex vivo study[J]. Osteoporos Int,2013, 24(9):2455-2460.
[12]JEHLE S, HULTER H N, KRAPF R. Effect of potassium citrate on bone density microarchitecture and fracture risk in healthy older adults without osteoporosis:A randomized controlled trial[J]. J Clin Endocrinol Metab, 2013, 98(1):207-217.