蛀食性害虫生长发育对小麦细胞膜透性及主要酶活性的影响
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摘要
研究玉米象和米象不同生长发育阶段侵害后对小麦籽粒细胞膜透性及主要酶活性的影响规律,为虫蚀小麦品质评价提供一定的量化指标和理论参考。选用2013年河南产的郑麦7698为试验材料,对两种害虫在卵、幼虫、蛹和成虫期侵害的小麦籽粒中丙二醛(MDA)含量、电导率、脂肪酶、过氧化氢酶(CAT)及过氧化物酶(POD)活性等指标进行测定,同时进行差异性分析。结果表明:玉米象和米象侵害后,小麦籽粒细胞膜系统遭受损伤,细胞膜脂质过氧化加剧,细胞膜通透性增大;小麦MDA含量和电导率逐渐升高,其中电导率从卵期到成虫期的增加幅度超过400%;脂肪酶活性呈现整体升高趋势;CAT和POD活性呈明显下降趋势;各指标数值在对照样与不同虫期之间存在着显著的差异,表明玉米象和米象不同生长发育阶段对小麦细胞膜透性和酶活性产生了显著性影响。
The effect of different growth states of maize weevil and rice weevil on the permeability of cell membrane and enzyme activity of wheat was studied in the present study,in order to provide certain quantitative indicators and theory reference for quality evaluation of insect damage wheat. Zhengmai 7698(2013) produced in Henan province was used as experimental material. The MDA content,electric conductivity and the activity of lipase,CAT and POD of wheat grain infested by the egg stage,larval stage,pupal stage,and adult stage of the two pests were measured respectively and the difference were also analyzed. The results showed that the cell membrane system of wheat grain was damaged, cell membrane lipid peroxidation was aggravated and permeability was increased after the wheat grain was infested by maize weevil and rice weevil. The MDA content and electric conductivity were increased gradually,and the electric conductivity was increased more than 400%from egg to adult. The lipase activity showed a trend of the overall rise,while the activity of CAT and POD had an opposite trend. The indexes values between the control group and experimental group had a significant difference,which indicated that maize weevil and rice weevil at different growth states had great influence on the cell membrane permeability and enzyme activity of wheat.
The effect of different growth states of maize weevil and rice weevil on the permeability of cell membrane and enzyme activity of wheat was studied in the present study,in order to provide certain quantitative indicators and theory reference for quality evaluation of insect damage wheat. Zhengmai 7698(2013) produced in Henan province was used as experimental material. The MDA content,electric conductivity and the activity of lipase,CAT and POD of wheat grain infested by the egg stage,larval stage,pupal stage,and adult stage of the two pests were measured respectively and the difference were also analyzed. The results showed that the cell membrane system of wheat grain was damaged, cell membrane lipid peroxidation was aggravated and permeability was increased after the wheat grain was infested by maize weevil and rice weevil. The MDA content and electric conductivity were increased gradually,and the electric conductivity was increased more than 400%from egg to adult. The lipase activity showed a trend of the overall rise,while the activity of CAT and POD had an opposite trend. The indexes values between the control group and experimental group had a significant difference,which indicated that maize weevil and rice weevil at different growth states had great influence on the cell membrane permeability and enzyme activity of wheat.
引文
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[2]周显青,张玉荣,王锋.稻谷储藏中细胞膜透性、膜脂过氧化及体内抗氧化酶活性变化[J].中国粮油学报,2008,23(2):159-162.
[3]傅家瑞,李卓杰,蔡东燕.花生种子劣变中超微结构的研究[J].植物生理学报,1983,9(1):93-101.
[4]张玉荣,周显青,张勇.储存玉米膜脂过氧化与生理指标的研究[J].中国农业科学,2008,41(10):3410-3414.
[5]白玉玲,鲁玉杰,王争艳,等.玉米象取食对新稻谷储藏品质的影响[J].河南工业大学学报(自然科学版),2012,33(6):52-56.
[6]叶霞,李学刚,张毅,等.稻谷中游离脂肪酸与脂肪酶活力的相关性[J].西南农业大学学报,2004,26(1):75-80.
[7]张玉荣,张鸿一,周显青,等.小麦受谷蠹侵害后其水分和容重变化研究[J].河南工业大学学报(自然科学版),2009,30(2):16-30.
[8]高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006.
[9]黄学林,陈润政.种子生理实验手册[M].北京:农业出版社,1990:155-157.
[10]周显青,张玉荣.储藏稻谷品质指标的变化及其差异性[J].农业工程学报,2008,24(12):238-241.
[11]陈建勋,王晓峰.植物生理学实验指导[M].广州:华南理工大学出版社,2002:124-126.
[12]刘莉,王若兰,王志山,等.基于图像处理的储藏小麦电导率变化的相关性研究[J].粮油食品科技,2014,22(4):95-97.
[13]罗龙海,范三红,张美祥,等.小麦种子过氧化物酶的纯化及其部分酶学性质[J].农业生物技术学报,2008,16(1):181-182.