河北峰峰矿区植物根围土壤微生物群落结构特征
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摘要
为探明煤矿区土壤微生物资源和生态分布特点,2016年10月从河北省邯郸市峰峰矿区采集冬青(Ilex chinensis)、小檗(Berberis kawakamii)和构树(Broussonetia papyrifera)根围土壤样品,利用磷脂脂肪酸(PLFA)法结合Sherlock微生物鉴定系统分析土壤微生物群落结构。结果表明:峰峰矿区植物根围土壤微生物PLFA种类丰富,其中以15∶0iso、16∶1ω7c、16∶0 10-methyl、18∶1ω9c和18∶1ω7c为优势PLFA;细菌为优势菌群。3种植物PLFA总量和微生物群落结构差异显著,表现为构树>小檗>冬青,其中14∶0anteiso和17∶1isoω9c为构树特有;18∶3ω6c为小檗特有;在冬青土壤未检测到15∶1isoω6c和18∶1ω5c等特征PLFA。RDA分析结果表明:微生物群落与土壤Zn、Mn、Pb、Cu、Cd元素及pH、全氮、磷酸酶和有机碳显著正相关,与速效磷显著负相关;土壤Cd、全氮、有机碳、速效磷和磷酸酶是土壤微生物PLFA的主要影响因子。冬青、小檗和构树的重金属富集能力较强,可作为矿区修复的理想植被。
To elucidate soil microbial resources and distribution in coal mine area,the rhizosphere soils of Ilex chinensis,Berberis kawakamii and Broussonetia papyrifera from Fengfeng mining area in Hebei Province,China were collected in October 2016.Soil microbial community structure in the rhizosphere of the three plant studied was analyzed by phospholipid fatty acid(PLFA)combined with Sherlock microbial identification system.The results showed that soil microbial PLFA species in Fengfeng Mining Area was rich,with.15∶0 iso,16∶1ω7 c,16∶0 10-methyl,18∶1ω9 cand 18∶1ω7 cas the dominant PLFAs,and Bacteria had a maximum value.Total PLFA and soil microbial community structure in rhizosphere soil of different plants were significantly different,characterized by B.papyrifera > B.kawakamii > I.chinensis.14∶0 anteiso and 17∶1 isoω9 cwere unique in rhizosphere of B.papyrifera,18∶3ω6 conly existed in the rhizosphere of B.kawakamii,and 15∶1 iso ω6 cand 18∶1 ω5 c were undetected in rhizosphere of I.chinensis.RDA indicated that soil microbial communities were significantly positively correlated with soil Zn,Mn,Pb,Cu,Cd,pH,total nitrogen,phosphatase and organic carbon,and was extremely negatively correlated to available phosphorous.The Cd,total nitrogen,total organic carbon,available phosphorus and phosphatase were the main influencing factors of soil microbial PLFA.Based on the ability to strongly accumulate heavy metals,three plant studied can be used to repair the mining area as ideal vegetation.
To elucidate soil microbial resources and distribution in coal mine area,the rhizosphere soils of Ilex chinensis,Berberis kawakamii and Broussonetia papyrifera from Fengfeng mining area in Hebei Province,China were collected in October 2016.Soil microbial community structure in the rhizosphere of the three plant studied was analyzed by phospholipid fatty acid(PLFA)combined with Sherlock microbial identification system.The results showed that soil microbial PLFA species in Fengfeng Mining Area was rich,with.15∶0 iso,16∶1ω7 c,16∶0 10-methyl,18∶1ω9 cand 18∶1ω7 cas the dominant PLFAs,and Bacteria had a maximum value.Total PLFA and soil microbial community structure in rhizosphere soil of different plants were significantly different,characterized by B.papyrifera > B.kawakamii > I.chinensis.14∶0 anteiso and 17∶1 isoω9 cwere unique in rhizosphere of B.papyrifera,18∶3ω6 conly existed in the rhizosphere of B.kawakamii,and 15∶1 iso ω6 cand 18∶1 ω5 c were undetected in rhizosphere of I.chinensis.RDA indicated that soil microbial communities were significantly positively correlated with soil Zn,Mn,Pb,Cu,Cd,pH,total nitrogen,phosphatase and organic carbon,and was extremely negatively correlated to available phosphorous.The Cd,total nitrogen,total organic carbon,available phosphorus and phosphatase were the main influencing factors of soil microbial PLFA.Based on the ability to strongly accumulate heavy metals,three plant studied can be used to repair the mining area as ideal vegetation.
引文
[1]束文圣,蓝崇钰.中国矿业废弃地的复垦对策研究(Ⅰ)[J].生态科学,2000,19(2):24-29.
[2] Anderson C R,Condron L M,Clough T J,et al.Biochar induced soil microbial community change:Implications for biogeochemical cycling of carbon,nitrogen and phosphorus[J].Pedobiologia,2011,54(5-6):309-320.
[3] Lagerstr9m A,Nilsson M C,Wardle D A.Decoupled responses of tree and shrub leaf and litter trait values to ecosystem retrogression across an island area gradient[J].Plant and Soil,2013,367(1-2):183-197.
[4] German D P,Chacon S S,Allison S D.Substrate concentration and enzyme allocation can affect rates of microbial decomposition[J].Ecology,2011,92(7):1471.
[5] Ushio M,Wagai R,Balser T C,et al.Variations in the soil microbial community composition of a tropical montane forest ecosystem:Does tree species matter[J].Soil Biology&Biochemistry,2008,40(10):2699-2702.
[6] Breulmann M,Schulz E,Weiβhuhn K,et al.Impact of the plant community composition on labile soil organic carbon,soil microbial activity and community structure in semi-natural grassland ecosystems of different productivity[J].Plant&Soil,2012,352(1-2):253-265.
[7]Wagai R,Kitayama K,Satomura T,et al.Interactive influences of climate and parent material on soil microbial community structure in Bornean tropical forest ecosystems[J].Ecological Research,2011,26(3):627-636.
[8] Bossio D A,Fleck J A,Scow K M,et al.Alteration of soil microbial communities and water quality in restored wetlands[J].Soil Biology&Biochemistry,2006,38(6):1223-1233.
[9]薛子可,左易灵,葛佳丽,等.西北典型荒漠样地花棒根际土壤微生物群落功能多样性[J].河北农业大学学报,2017,40(3):66-71.
[10]鲁如坤.土壤农业化学分析方法[M].北京:中国农业科技出版社,2000.
[11] Bossio D A,Scow K M.Impacts of carbon and flooding on soil microbial communities:phospholipid fatty acid profiles and substrate utilization patterns[J].Microbial Ecology,1998,35(3):265.
[12] Frostegrd A,Bth E.The use of phospholipid fatty acid analysis to estimate bacterial and fungal biomass in soil[J].Biology&Fertility of Soils,1996,22(1-2):59-65.
[13] Abaye D A,Lawlor K,Hirsch P R,et al.Changes in the microbial community of an arable soil caused by long-term metal contamination.[J].European Journal of Soil Science,2005,56(1):93-102.
[14]张秋芳,刘波,林营志,等.土壤微生物群落磷脂脂肪酸PLFA生物标记多样性[J].生态学报,2009,29(8):4127-4137.
[15]左易灵,贺学礼,王少杰,等.磷脂脂肪酸(PLFA)法检测蒙古沙冬青根围土壤微生物群落结构[J].环境科学,2016,37(7):2705-2713.
[16] Avidano L,Gamalero E,Cossa G P,et al.Characterization of soil health in an Italian polluted site by using microorganisms as bioindicators[J].Applied Soil Ecology,2005,30(1):21-33.
[17]洪晨,邢奕,司艳晓,等.铁矿区内重金属对土壤氨氧化微生物群落组成的影响[J].中国环境科学,2014,34(5):1212-1221.
[18] Ma Y,Prasad M N V,Rajkumar M,et al.Plant growth promoting rhizobacteria and endophytes accelerate phytoremediation of metalliferous soils[J].Biotechnology Advances,2011,29(2):248.
[19]刘维涛,张银龙,陈喆敏,等.矿区绿化树木对镉和锌的吸收与分布[J].应用生态学报,2008,19(4):752-756.
[20]童方平,龙应忠,杨勿享,等.锑矿区构树富集重金属的特性研究[J].中国农学通报,2010,26(14):328-331.
[21] Kim J H,Campbell B C,Mahoney N,et al.Enhanced activity of strobilurin and fludioxonil by using berberine and phenolic compounds to target fungal antioxidative stress response[J].Letters in Applied Microbiology,2007,45(2):134-141.
[22]宋贤冲,曹继钊,唐健,等.猫儿山常绿阔叶林不同土层土壤微生物群落功能多样性[J].生态科学,2015,34(6):93-99.
[23]刘秉儒.贺兰山东坡典型植物群落土壤微生物量碳、氮沿海拔梯度的变化特征[J].生态环境学报,2010,19(4):883-888.
[24]杨佳佳,安韶山,张宏,等.黄土丘陵区小流域侵蚀环境对土壤微生物量及酶活性的影响[J].生态学报,2015,35(17):5666-5674.
[25] Simona C,Angela R F,Amalia V D S.Suitability of soil microbial parameters as indicators of heavy metal pollution[J].Water Air&Soil Pollution,2004,158(1):21-35.
[26]刘玉燕,刘敏,刘浩峰.城市土壤重金属污染特征分析[J].土壤通报,2006,37(1):184-188
[2] Anderson C R,Condron L M,Clough T J,et al.Biochar induced soil microbial community change:Implications for biogeochemical cycling of carbon,nitrogen and phosphorus[J].Pedobiologia,2011,54(5-6):309-320.
[3] Lagerstr9m A,Nilsson M C,Wardle D A.Decoupled responses of tree and shrub leaf and litter trait values to ecosystem retrogression across an island area gradient[J].Plant and Soil,2013,367(1-2):183-197.
[4] German D P,Chacon S S,Allison S D.Substrate concentration and enzyme allocation can affect rates of microbial decomposition[J].Ecology,2011,92(7):1471.
[5] Ushio M,Wagai R,Balser T C,et al.Variations in the soil microbial community composition of a tropical montane forest ecosystem:Does tree species matter[J].Soil Biology&Biochemistry,2008,40(10):2699-2702.
[6] Breulmann M,Schulz E,Weiβhuhn K,et al.Impact of the plant community composition on labile soil organic carbon,soil microbial activity and community structure in semi-natural grassland ecosystems of different productivity[J].Plant&Soil,2012,352(1-2):253-265.
[7]Wagai R,Kitayama K,Satomura T,et al.Interactive influences of climate and parent material on soil microbial community structure in Bornean tropical forest ecosystems[J].Ecological Research,2011,26(3):627-636.
[8] Bossio D A,Fleck J A,Scow K M,et al.Alteration of soil microbial communities and water quality in restored wetlands[J].Soil Biology&Biochemistry,2006,38(6):1223-1233.
[9]薛子可,左易灵,葛佳丽,等.西北典型荒漠样地花棒根际土壤微生物群落功能多样性[J].河北农业大学学报,2017,40(3):66-71.
[10]鲁如坤.土壤农业化学分析方法[M].北京:中国农业科技出版社,2000.
[11] Bossio D A,Scow K M.Impacts of carbon and flooding on soil microbial communities:phospholipid fatty acid profiles and substrate utilization patterns[J].Microbial Ecology,1998,35(3):265.
[12] Frostegrd A,Bth E.The use of phospholipid fatty acid analysis to estimate bacterial and fungal biomass in soil[J].Biology&Fertility of Soils,1996,22(1-2):59-65.
[13] Abaye D A,Lawlor K,Hirsch P R,et al.Changes in the microbial community of an arable soil caused by long-term metal contamination.[J].European Journal of Soil Science,2005,56(1):93-102.
[14]张秋芳,刘波,林营志,等.土壤微生物群落磷脂脂肪酸PLFA生物标记多样性[J].生态学报,2009,29(8):4127-4137.
[15]左易灵,贺学礼,王少杰,等.磷脂脂肪酸(PLFA)法检测蒙古沙冬青根围土壤微生物群落结构[J].环境科学,2016,37(7):2705-2713.
[16] Avidano L,Gamalero E,Cossa G P,et al.Characterization of soil health in an Italian polluted site by using microorganisms as bioindicators[J].Applied Soil Ecology,2005,30(1):21-33.
[17]洪晨,邢奕,司艳晓,等.铁矿区内重金属对土壤氨氧化微生物群落组成的影响[J].中国环境科学,2014,34(5):1212-1221.
[18] Ma Y,Prasad M N V,Rajkumar M,et al.Plant growth promoting rhizobacteria and endophytes accelerate phytoremediation of metalliferous soils[J].Biotechnology Advances,2011,29(2):248.
[19]刘维涛,张银龙,陈喆敏,等.矿区绿化树木对镉和锌的吸收与分布[J].应用生态学报,2008,19(4):752-756.
[20]童方平,龙应忠,杨勿享,等.锑矿区构树富集重金属的特性研究[J].中国农学通报,2010,26(14):328-331.
[21] Kim J H,Campbell B C,Mahoney N,et al.Enhanced activity of strobilurin and fludioxonil by using berberine and phenolic compounds to target fungal antioxidative stress response[J].Letters in Applied Microbiology,2007,45(2):134-141.
[22]宋贤冲,曹继钊,唐健,等.猫儿山常绿阔叶林不同土层土壤微生物群落功能多样性[J].生态科学,2015,34(6):93-99.
[23]刘秉儒.贺兰山东坡典型植物群落土壤微生物量碳、氮沿海拔梯度的变化特征[J].生态环境学报,2010,19(4):883-888.
[24]杨佳佳,安韶山,张宏,等.黄土丘陵区小流域侵蚀环境对土壤微生物量及酶活性的影响[J].生态学报,2015,35(17):5666-5674.
[25] Simona C,Angela R F,Amalia V D S.Suitability of soil microbial parameters as indicators of heavy metal pollution[J].Water Air&Soil Pollution,2004,158(1):21-35.
[26]刘玉燕,刘敏,刘浩峰.城市土壤重金属污染特征分析[J].土壤通报,2006,37(1):184-188