西藏羊肚菌水提物的抗氧化活性研究
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摘要
目的:研究不同浓度的羊肚菌水提物对秀丽隐杆线虫寿命的影响。方法:将秀丽隐杆线虫分别培养在普通培养基及添加不同浓度(0 mg/mL、25 mg/mL、100 mg/mL、200 mg/mL)羊肚菌水提物的NGM培养基,研究羊肚菌水提物对秀丽隐杆线虫寿命、产卵量、体内脂褐素含量(ROS)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活力的影响。结果:不同浓度羊肚菌水提物能够促使秀丽隐杆线虫平均寿命分别延长4.15%、34.11%、33.21%;线虫产卵量随着羊肚菌水提物浓度的增加而增加;羊肚菌水提物能够显著降低线虫体内ROS积累量(p <0.05);另外,羊肚菌水提物能够显著提高秀丽隐杆线虫体内SOD、CAT酶的活力。结论:羊肚菌水提物能够显著延长秀丽隐杆线虫的寿命,有效延缓衰老,提高产卵量,改善机体的抗氧化能力。
The effects of different concentrations of water extracts of ampuls on the life of elegans were studied.Cryptonematodes elegans were cultured in ordinary medium and NGM medium added with different concentrations(0,25, 100, 200 mg/mL). To study the effects of Aqua sulpii on the life span, spawning volume, lipopolicin content(ROS),superoxide dismutase(SOD) and catalase(CAT) activity of Cryptococcus elegans. The results showed that different concentrations of water extracts could increase the life expectancy of Cryptococcus elegans by 4.15%, 34.11% and 33.21% respectively. The number of eggs produced by nematodes increased with the increase of water extract concentration.The water extracts of the gut bacteria can significantly reduce the amount of ROS accumulation in nematodes(p < 0.05);In addition, the water extract of the tripe can significantly increase the activity of SOD and CATase in the body of Cryptococcus elegans. The water extract of the gut can significantly prolong the life span of Cryptococcus elegans,effectively delay aging, increase the number of eggs produced, and improve the body's antioxidant ability.
The effects of different concentrations of water extracts of ampuls on the life of elegans were studied.Cryptonematodes elegans were cultured in ordinary medium and NGM medium added with different concentrations(0,25, 100, 200 mg/mL). To study the effects of Aqua sulpii on the life span, spawning volume, lipopolicin content(ROS),superoxide dismutase(SOD) and catalase(CAT) activity of Cryptococcus elegans. The results showed that different concentrations of water extracts could increase the life expectancy of Cryptococcus elegans by 4.15%, 34.11% and 33.21% respectively. The number of eggs produced by nematodes increased with the increase of water extract concentration.The water extracts of the gut bacteria can significantly reduce the amount of ROS accumulation in nematodes(p < 0.05);In addition, the water extract of the tripe can significantly increase the activity of SOD and CATase in the body of Cryptococcus elegans. The water extract of the gut can significantly prolong the life span of Cryptococcus elegans,effectively delay aging, increase the number of eggs produced, and improve the body's antioxidant ability.
引文
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[3]卢可可,谭玉荣,吴素蕊,等.不同产地尖顶羊肚菌多酚组成及抗氧化活性研究[J].食品科学,2015(7):6-12.
[4]李谣,陈金龙,王丽颖,等.羊肚菌多糖抑制人乳腺癌细胞MDA-MB-231增殖和诱导细胞凋亡研究[J].食品科学,2016(21):214-218.
[5]罗霞,魏巍,余梦瑶,等.尖顶羊肚菌对急性酒精性胃黏膜损伤保护作用研究[J].菌物学报,2011,30(2):319-324.
[6]吴映明,陈奋,林建新,等.羊肚菌对小鼠小肠推进功能的研究[J].广东第二师范学院学报,2005,25(3):80-82.
[7]殷伟伟,张松,吴金凤.尖顶羊肚菌活性提取物降血脂作用的研究[J].菌物学报,2009,28(6):873-877.
[8]Kalyoncu F,Oskay M,Sa?lam H,et al.Antimicrobial and Antioxidant Activities of Mycelia of 10 Wild Mushroom Species[J].Journal of Medicinal Food,2010,13(2):415-419.
[9]Kim J A,Lau E,Tay D,et al.Antioxidant and NF-κB Inhibitory Constituents Isolated from Morchella esculenta[J].Natural Product Research,2011,25(15):1412-1417.
[10]赵英,刘晓慧,刘小翠,等.羊肚菌等9种食用菌抗氧化活性研究[J].山西大同大学学报(自然科学版),2017,33(2):28-32.
[11]孙巧弟,张江萍,谢洋洋,等.羊肚菌营养素、功能成分和保健功能的研究进展[J].食品科学,2019,40(5):1-8.
[12]杨番,夏程程,钟晓凌,等.秀丽隐杆线虫模型在食品营养评价中的研究应用[J].食品科学,2018:1-12.
[13]Zhou Y,Shao L,Jian L I,et al.An Efficient and Novel Screening Model for Assessing the Bioactivity of Extracts against Multidrug-Resistant Using[J].Bioscience Biotechnology&Biochemistry,2014,75(9):1746-1751.
[14]Jiang Y,Chen J,Wu Y,et al.Sublethal Toxicity Endpoints of Heavy Metals to the Nematode Caenorhabditis elegans[J].Plos One,2016,11(1):e148014.
[15]陆沛,夏世金,孙涛,等.白藜芦醇对秀丽线虫衰老的影响[J].成都医学院学报,2012(3):359-363.
[16]Katiki L M,Ferreira J F,Gonzalez J M,et al.Anthelmintic Effect of Plant Extracts Containing Condensed and Hydrolyzable Tannins on Caenorhabditis elegans,and their Antioxidant Capacity[J].Veterinary Parasitology,2013,192(1-3):218-227.
[17]Gao D W,Wei-Guang L I,Gao Y,et al.Caenorhabditis elegans:a model organism for studies of antioxidant activity of plant extracts[J].Military Medical Sciences,2013,37(4):120-315.
[18]Van Raamsdonk J M,Hekimi S.Reactive Oxygen Species and Aging in Caenorhabditis elegans:Causal or Casual Relationship?[J].Antioxidants&Redox Signaling,2010,13(12):1911-1953.
[19]Wu Z,Smith J V,Paramasivam V,et al.Ginkgo Biloba Extract EGb 761 Increases Stress Resistance and Extends Life Span of Caenorhabditis elegans[J].Cell Mol Biol(Noisy-le-grand),2002,48(6):725-731.