镉胁迫对东营野生大豆幼苗抗氧化系统及可溶性蛋白的影响
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摘要
【目的】为综合评价东营野生大豆耐镉胁迫的能力。【方法】以东营野生大豆为材料,检测不同浓度镉(Cd)胁迫下野生大豆抗氧化酶活性、丙二醛含量及可溶性蛋白含量的变化。【结果】丙二醛含量随Cd浓度增加而增加。当Cd浓度低于8 mg/L时,抗氧化酶(SOD和CAT)活性、可溶性蛋白含量均高于对照;当Cd浓度为16 mg/L时,抗氧化酶活性、可溶性蛋白含量均显著低于对照。【结论】当Cd浓度小于等于8.0 mg/L时,野生大豆可通过自身调节减轻或缓解镉胁迫带来的不利影响。
【Objective】To comprehensively evaluate the cadmium resistant ability of Dongying wild soybean.【Method】Using Dongying wild soybean as the materials,the antioxidant enzyme activities,the contents of malondialdehyde and soluble portein were detected under different treatments of cadmium concentrations.【Result】The contents of malondialdehyde were increased with increasing Cd concentrations. When Cd concentrations were lower than 8 mg/L,the antioxidant enzyme activities(SOD and CAT) and the contents of soluble protein under Cd stress were significantly higher than that of the control;when Cd concentration was 16 mg/L,the activity of antioxidant enzymes and the content of soluble protein were significantly lower than that of the control.【Conclusion】When Cd concentrations were less than or equal to 8.0 mg/L,wild soybean could mitigate or alleviate the adverse effects of Cd stress through self-regulation.
【Objective】To comprehensively evaluate the cadmium resistant ability of Dongying wild soybean.【Method】Using Dongying wild soybean as the materials,the antioxidant enzyme activities,the contents of malondialdehyde and soluble portein were detected under different treatments of cadmium concentrations.【Result】The contents of malondialdehyde were increased with increasing Cd concentrations. When Cd concentrations were lower than 8 mg/L,the antioxidant enzyme activities(SOD and CAT) and the contents of soluble protein under Cd stress were significantly higher than that of the control;when Cd concentration was 16 mg/L,the activity of antioxidant enzymes and the content of soluble protein were significantly lower than that of the control.【Conclusion】When Cd concentrations were less than or equal to 8.0 mg/L,wild soybean could mitigate or alleviate the adverse effects of Cd stress through self-regulation.
引文
[1]四川省环保厅和国土厅.四川省土壤污染状况调查公报[Z].2014-11-28.
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[3]高天然,周可新.镉胁迫下水生动物的生物标志物研究进展[J].生态与农村环境学报,2017, 33(4):297-307.
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[11]张明荣,吴海英,吴迅,等.四川大豆主产区产业现状、存在的问题及发展对策[J].大豆科技,2009(5):6-8.
[12]冯军,吴海英,于晓波,等.2017年四川大豆生产技术指导意见[J].大豆科技,2017(3):43-44.
[13]李桂兰,张悦,乔亚科,等.冀东沿海地区野生大豆过氧化物酶同工酶多样性研究[J].中国油料作物学报,2009, 31(3):386-390.
[14]陈佳月,解静芳,姜洪进,等.草甘膦与镉复合胁迫对玉米幼苗抗氧化酶活性及光合作用的影响[J].生态毒理学报,2018,I(13):219-228.
[15]陈浩东,贺云新,郭利双,等.镉胁迫对3个棉花品种生理生化特征及农艺性状的影响[J].棉花学报,2018, 30(1):62-70.
[16]李妍.铅镉胁迫对小麦幼苗抗氧化酶活性及丙二醛含量的影响[J].麦类作物学报,2009, 29(3):514-517.
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[18] MOBIN M, KHAN N A. Photosynthetic activity, pigment composition and antioxidative response of two mustard(Brassica juncea)cultivars differing in photosynthetic capacity subjected to cadmium stress[J].Journal of Plant Physiology,2007,164(5):601-610.
[19]张泽锦,唐丽,李跃建,等.四川地区低镉富集蔬菜品种分析及安全性评估[J].西南农业学报,2016, 29(10):2483-2487.
[20]孙月美,宁国辉,刘树庆,等.耐受性植物油葵和棉花对镉的富集特征研究[J].水土保持学报,2015, 29(6):281-286.
[21]顾翠花,王懿祥,白尚斌,等.四种园林植物对土壤镉污染的耐受性[J].生态学报,2015, 35(8):2536-2544.
[22]王志坤,廖柏寒,黄运湘,等.镉胁迫对大豆幼苗生长影响及不同品种耐镉差异性研究[J].农业环境科学学报,2006,25(5):1143-1147.
[23] WANG X, CHEN H, SHA A, et al. Laboratory testing and molecular analysis of the resistance of wild and cultivated soybeans to cotton bollworm,Helicoverpa armigera(Hubner)[J]. The Crop Journal Calls for Papers,2015,3(1):19-28.
[24]陈爱国,吴禹,崔晓光,等.不同栽培大豆和野生大豆品种抗旱性综合评价[J].辽宁农业科学,2014(3):1-5.
[25]赵云云,钟彩霞,方小龙,等.华南地区夏播大豆品种镉耐性及籽粒镉积累的差异[J].大豆科学,2013, 3(32):336-340.
[26]黄运湘,廖柏寒,王志坤,等.不同大豆品种镉毒害效应及耐镉差异性[J].湖南农业大学学报(自然科学版),2008, 5(34):519-524.
[27] ZAIB UN, CHEN C, YANG Y, et al. Constitutive overexpression of myo-inositol-1-phosphate synthase gene(GsMIPS2)from Glycine soja confers enhanced salt tolerance at various growth stages in Arabidopsis[J]. Journal of Northeast Agricultural University, 2016, 23(2):28-44.
[28]沈文飚,徐朗莱,叶茂炳,等.氮蓝四唑光化还原法测定超氧化物歧化酶活性的适宜条件[J].南京农业大学学报,1996,19(2):101-102.
[29]李忠光,龚明.愈创木酚法测定植物过氧化物酶活性的改进[J].植物生理学通讯,2008, 44(2):323-324.
[30]杨兰芳,庞静,彭小兰,等.紫外分光光度法测定植物过氧化氢酶活性[J].现代农业科技,2009(20):364-366.
[31]焦洁.考马斯亮蓝G-250染色法测定苜蓿中可溶性蛋白含量[J].农业工程技术,2016, 36(17):33-34.
[32]赵世杰,许长成,邹琦,等.植物组织中丙二醛测定方法的改进[J].植物生理学通讯,1994, 30(3):207-210.
[33]许树成,丁海东.重金属Cd、Zn污染对番茄果实品质的影响及其残留的研究[J].阜阳师范学院学报(自然科学版),2007,24(4):15-18.
[34]刘晓庆,陈华涛,张红梅,等.不同品种大豆幼苗对镉胁迫的响应[J].江西农业学报,2017, 29(3):14-17.
[35]覃勇荣,冯济梅,梁文忠,等.镉胁迫下的桑树幼苗叶片丙二醛含量动态分析[J].河池学院学报,2012, 32(2):17-21.
[36]曲丹阳,顾万荣,张立国,等.壳聚糖对镉胁迫下玉米幼苗根系抗氧化酶活性和内源激素水平的影响[J].西北植物学报,2017, 37(4):719-727.
[37]郑世英,王丽燕,张海英.镉胁迫对两个大豆品种抗氧化酶活性及丙二醛含量的影响[J].江苏农业科学,2007, 35(5):53-55.
[38]陈维,金晨钟,吴晓峰,等.不同浓度镉胁迫对稗草生理特性的影响[J].亚热带植物科学,2016,45(3):207-211.
[39]杨叶萍,简敏菲,余厚平,等.镉胁迫对苎麻(Boehmeria nivea)根系及叶片抗氧化系统的影响[J].生态毒理学报,2016, 11(4):184-193.
[40]张琼,陆銮眉,戴清霞,等.镉胁迫对水仙根系抗氧化系统的影响[J].福建农业学报,2016, 31(6):591-595.
[41]王霞,吴霞,马亮,等.棉花幼苗受铅、镉胁迫的抗氧化酶反应[J].江苏农业科学,2012, 40(12):105-107.
[42]王宇涛,陈志勇,曾琬淋,等.拟南芥对镉胁迫的生理响应[J].华南师范大学学报(自然科学版),2014, 46(2):99-107.
[43]徐勤松,施国新.镉及镉、锌共同作用对水芹菜部分生理特性的影响[J].南京师大学报(自然科学版),2000,23(4):97-100.
[44]易冕,张饮江,董悦,等.3种沉水植物对镉的生理特性响应[J].安全与环境学报,2013, 13(1):9-14.
[45] GALLEGO S M, BENAVIDES M P, TOMARO M L. Effect of cadmium ions on antioxidant defense system in sunflower cotyledons[J]. Biologia Plantarum, 1999, 42(1):49-55.
[46]王嘉玥,刘欣萍,吴航枫,等.镉胁迫对旱柳植株活性氧含量的影响[J].现代农业科技,2016(15):191-193.
[47]徐婷婷,秦丽,王吉秀,等.人工镉增补对续断菊抗氧化酶活性及可溶性蛋白和丙二醛含量的影响[J].云南农业大学学报(自然科学),2014, 29(3):409-414.
[48]刘晓.不同品种烟草忍耐镉的机制研究[D].重庆:西南大学,2007.
[2]茹淑华,苏德纯,王激清.土壤镉污染特征及污染土壤的植物修复技术机理[J].中国生态农业学报,2006,14(4):29-33.
[3]高天然,周可新.镉胁迫下水生动物的生物标志物研究进展[J].生态与农村环境学报,2017, 33(4):297-307.
[4]史静,潘根兴,夏运生,等.镉胁迫对两品种水稻生长及抗氧化酶系统的影响[J].生态环境学报,2013, 22(5):832-837.
[5] KHAN N A, AHMAD I, SINGH S, et al. Variation in growth,photosynthesis and yield of five wheat cultivars exposed to cadmium stress[J]. World Journal of Agricultural Sciences, 2006,2(2):223-226.
[6]宋建,金凤媚,薛俊,等.镉胁迫对植物生长及生理生态效应的研究进展[J].天津农业科学,2014, 20(12):19-22.
[7] BARCELO J, POSCHENRIEDER C. Plant water relations as affected by heavy metal stress:A review[J]. Journal of Plant Nutrition, 1990, 13(1):1-37.
[8]吴甘霖.镉对花生幼苗生长及生理生态特性的影响[J].生物学杂志,2008, 25(5):31-33.
[9]张成,李浩,任树友,等.不同小麦品种籽粒对土壤镉吸收差异性的初步研究[J].四川农业科技,2016(9):39-41.
[10]李沛然,龚颖婷,黄丽颖,等.大豆镉累积及吸收转运特性研究[J].广东农业科学,2016, 43(5):82-86.
[11]张明荣,吴海英,吴迅,等.四川大豆主产区产业现状、存在的问题及发展对策[J].大豆科技,2009(5):6-8.
[12]冯军,吴海英,于晓波,等.2017年四川大豆生产技术指导意见[J].大豆科技,2017(3):43-44.
[13]李桂兰,张悦,乔亚科,等.冀东沿海地区野生大豆过氧化物酶同工酶多样性研究[J].中国油料作物学报,2009, 31(3):386-390.
[14]陈佳月,解静芳,姜洪进,等.草甘膦与镉复合胁迫对玉米幼苗抗氧化酶活性及光合作用的影响[J].生态毒理学报,2018,I(13):219-228.
[15]陈浩东,贺云新,郭利双,等.镉胁迫对3个棉花品种生理生化特征及农艺性状的影响[J].棉花学报,2018, 30(1):62-70.
[16]李妍.铅镉胁迫对小麦幼苗抗氧化酶活性及丙二醛含量的影响[J].麦类作物学报,2009, 29(3):514-517.
[17] COBBETT C S. Phytochelatins and their roles in heavy metal detoxification[J]. Plant Physiology,2000,123(3):825-832.
[18] MOBIN M, KHAN N A. Photosynthetic activity, pigment composition and antioxidative response of two mustard(Brassica juncea)cultivars differing in photosynthetic capacity subjected to cadmium stress[J].Journal of Plant Physiology,2007,164(5):601-610.
[19]张泽锦,唐丽,李跃建,等.四川地区低镉富集蔬菜品种分析及安全性评估[J].西南农业学报,2016, 29(10):2483-2487.
[20]孙月美,宁国辉,刘树庆,等.耐受性植物油葵和棉花对镉的富集特征研究[J].水土保持学报,2015, 29(6):281-286.
[21]顾翠花,王懿祥,白尚斌,等.四种园林植物对土壤镉污染的耐受性[J].生态学报,2015, 35(8):2536-2544.
[22]王志坤,廖柏寒,黄运湘,等.镉胁迫对大豆幼苗生长影响及不同品种耐镉差异性研究[J].农业环境科学学报,2006,25(5):1143-1147.
[23] WANG X, CHEN H, SHA A, et al. Laboratory testing and molecular analysis of the resistance of wild and cultivated soybeans to cotton bollworm,Helicoverpa armigera(Hubner)[J]. The Crop Journal Calls for Papers,2015,3(1):19-28.
[24]陈爱国,吴禹,崔晓光,等.不同栽培大豆和野生大豆品种抗旱性综合评价[J].辽宁农业科学,2014(3):1-5.
[25]赵云云,钟彩霞,方小龙,等.华南地区夏播大豆品种镉耐性及籽粒镉积累的差异[J].大豆科学,2013, 3(32):336-340.
[26]黄运湘,廖柏寒,王志坤,等.不同大豆品种镉毒害效应及耐镉差异性[J].湖南农业大学学报(自然科学版),2008, 5(34):519-524.
[27] ZAIB UN, CHEN C, YANG Y, et al. Constitutive overexpression of myo-inositol-1-phosphate synthase gene(GsMIPS2)from Glycine soja confers enhanced salt tolerance at various growth stages in Arabidopsis[J]. Journal of Northeast Agricultural University, 2016, 23(2):28-44.
[28]沈文飚,徐朗莱,叶茂炳,等.氮蓝四唑光化还原法测定超氧化物歧化酶活性的适宜条件[J].南京农业大学学报,1996,19(2):101-102.
[29]李忠光,龚明.愈创木酚法测定植物过氧化物酶活性的改进[J].植物生理学通讯,2008, 44(2):323-324.
[30]杨兰芳,庞静,彭小兰,等.紫外分光光度法测定植物过氧化氢酶活性[J].现代农业科技,2009(20):364-366.
[31]焦洁.考马斯亮蓝G-250染色法测定苜蓿中可溶性蛋白含量[J].农业工程技术,2016, 36(17):33-34.
[32]赵世杰,许长成,邹琦,等.植物组织中丙二醛测定方法的改进[J].植物生理学通讯,1994, 30(3):207-210.
[33]许树成,丁海东.重金属Cd、Zn污染对番茄果实品质的影响及其残留的研究[J].阜阳师范学院学报(自然科学版),2007,24(4):15-18.
[34]刘晓庆,陈华涛,张红梅,等.不同品种大豆幼苗对镉胁迫的响应[J].江西农业学报,2017, 29(3):14-17.
[35]覃勇荣,冯济梅,梁文忠,等.镉胁迫下的桑树幼苗叶片丙二醛含量动态分析[J].河池学院学报,2012, 32(2):17-21.
[36]曲丹阳,顾万荣,张立国,等.壳聚糖对镉胁迫下玉米幼苗根系抗氧化酶活性和内源激素水平的影响[J].西北植物学报,2017, 37(4):719-727.
[37]郑世英,王丽燕,张海英.镉胁迫对两个大豆品种抗氧化酶活性及丙二醛含量的影响[J].江苏农业科学,2007, 35(5):53-55.
[38]陈维,金晨钟,吴晓峰,等.不同浓度镉胁迫对稗草生理特性的影响[J].亚热带植物科学,2016,45(3):207-211.
[39]杨叶萍,简敏菲,余厚平,等.镉胁迫对苎麻(Boehmeria nivea)根系及叶片抗氧化系统的影响[J].生态毒理学报,2016, 11(4):184-193.
[40]张琼,陆銮眉,戴清霞,等.镉胁迫对水仙根系抗氧化系统的影响[J].福建农业学报,2016, 31(6):591-595.
[41]王霞,吴霞,马亮,等.棉花幼苗受铅、镉胁迫的抗氧化酶反应[J].江苏农业科学,2012, 40(12):105-107.
[42]王宇涛,陈志勇,曾琬淋,等.拟南芥对镉胁迫的生理响应[J].华南师范大学学报(自然科学版),2014, 46(2):99-107.
[43]徐勤松,施国新.镉及镉、锌共同作用对水芹菜部分生理特性的影响[J].南京师大学报(自然科学版),2000,23(4):97-100.
[44]易冕,张饮江,董悦,等.3种沉水植物对镉的生理特性响应[J].安全与环境学报,2013, 13(1):9-14.
[45] GALLEGO S M, BENAVIDES M P, TOMARO M L. Effect of cadmium ions on antioxidant defense system in sunflower cotyledons[J]. Biologia Plantarum, 1999, 42(1):49-55.
[46]王嘉玥,刘欣萍,吴航枫,等.镉胁迫对旱柳植株活性氧含量的影响[J].现代农业科技,2016(15):191-193.
[47]徐婷婷,秦丽,王吉秀,等.人工镉增补对续断菊抗氧化酶活性及可溶性蛋白和丙二醛含量的影响[J].云南农业大学学报(自然科学),2014, 29(3):409-414.
[48]刘晓.不同品种烟草忍耐镉的机制研究[D].重庆:西南大学,2007.