疮灵液对鼠永生化黑素细胞黑素生成的影响
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摘要
目的:观察疮灵液对鼠永生化细胞(ATCC~?CRL-2770TM)黑素细胞黑素生成的作用。方法:应用MTT法测鼠永生化黑素细胞增殖、多巴氧化法测鼠永生化黑素细胞内酪氨酸酶活性、NaOH裂解法测鼠永生化黑素细胞黑素含量。结果:疮灵液在100～1 600μg/mL剂量,对小鼠永生化黑素细胞增殖具有增进作用,400μg/mL剂量促进作用最明显(P<0.05)。疮灵液为800μg/mL～3 200μg/mL剂量,可显著激活鼠永生化黑素细胞酪氨酸酶活性(P<0.01)。疮灵液于100μg/mL～3 200μg/mL剂量,对鼠永生化黑素细胞黑素合成均有增进作用(P<0.05)。结论:疮灵液对鼠永生化黑素细胞黑素的合成具有显著增进作用,并且在治疗诸如白癜风的色素疾病中具有潜在的应用。
Objective: To observe the effect of Chuangling Liquid on the melanogenesis of melanocytes in rat immortalized cells(ATCC~? CRL-2770 TM). Methods: Melanocyte proliferation was determined by MTT assay, tyrosinase activity of melanocyte was detected by dopa oxidation method, and melanin content was measured by NaOH lysis method. Results: When the concentration of Chuangling Liquid was at 100～1 600 μg/mL, it promoted the proliferation of melanocytes, and the promotion effect was most obvious at 400 μg/mL(P<0.05). Tyrosinase activity was significantly activated when the concentration of Chuangling Liquid was at 400 μg/mL(P<0.01). When the concentration of Chuangling Liquid was at 100～3 200 μg/mL, it promoted melanin synthesis(P<0.05). Conclusion: Chuangling Liquid has a promoting effect on melanin synthesis of melanocyte, which has a promising effect on leukoderma of hypopigmentation disease.
Objective: To observe the effect of Chuangling Liquid on the melanogenesis of melanocytes in rat immortalized cells(ATCC~? CRL-2770 TM). Methods: Melanocyte proliferation was determined by MTT assay, tyrosinase activity of melanocyte was detected by dopa oxidation method, and melanin content was measured by NaOH lysis method. Results: When the concentration of Chuangling Liquid was at 100～1 600 μg/mL, it promoted the proliferation of melanocytes, and the promotion effect was most obvious at 400 μg/mL(P<0.05). Tyrosinase activity was significantly activated when the concentration of Chuangling Liquid was at 400 μg/mL(P<0.01). When the concentration of Chuangling Liquid was at 100～3 200 μg/mL, it promoted melanin synthesis(P<0.05). Conclusion: Chuangling Liquid has a promoting effect on melanin synthesis of melanocyte, which has a promising effect on leukoderma of hypopigmentation disease.
引文
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[16] 张媞.疮灵液基于解毒收敛改善浆细胞性乳腺炎疮面炎症以促进愈合临床研究[D].南京:南京中医药大学,2017.
[2] Ma ZQ,Hu H,He TT,et al.An Assessment Of Traditional Uighur Medicine In Current Xinjiang Region (China)[J].Afr J Tradit Complement Altern Med.2014,11(2):301-314.
[3] Huang X,Ishikawa M,Mansur A,et al.The Effects of Bairesi Complex Prescription (a Uyghur Medicine Prescription) and Its Five Crude Herbal Extracts on Melanogenesis in G-361 Cells[J].Evid Based Complement Alternat Med,2016,(2016):1-7.
[4] 姚昶,江虹,许岩磊,等.疮灵液载入胶原促进慢性创面的愈合[J].中国组织工程研究,2015,19(34):5518-5522.
[5] 彭琬苏.疮灵液对黑素细胞黑素合成的影响[D].南京:南京中医药大学,2016.
[6] Bleuel R,Eberlein B.Therapeutic management of vitiligo[J].J Dtsch Dermatol Ges,2018,16(11):1309-1313.
[7] Bidaki R,Majidi N,Moghadam Ahmadi A,et al.Vitiligo and social acceptance[J].Clin Cosmet Investig Dermatol,2018,11:383-386.
[8] 张春艳,许爱娥.治疗白癜风的中药外用制剂总结分析[J].中华中医药学刊,2012,30(9):2074-2077.
[9] Simon JD,Peles D,Wakamatsu K,et al.Current challenges in understanding melanogenesis:bridging chemistry,biological control,morphology,and function[J].Pigment cell & Melanoma Research,2009,22(5):563-579.
[10] 许振毅,张庆红.大黄对人表皮黑素细胞的增殖及对酪氨酸酶的双向调节作用[J].中华皮肤科杂志,1997(6):46-47.
[11] 张理平,张海燕,李孝栋,等.28味中药酸性成分提取物影响黑素合成的实验研究[J].中华中医药杂志,2009,24(11):1443-1445.
[12] 姜丽莎,张国斌,王恩波,等.复方红花酊对白癜风豚鼠模型TNF-α和IL-10表达的影响[J].皮肤科学通报,2017,34(6):714-721.
[13] Mou K,Liu W,Miao Y,et al.HMGB1 deficiency reduces H2O2-induced oxidative damage in human melanocytes via the Nrf2 pathway[J].J Cell Mol Med,2018,22(12):6148-6156.
[14] 黎望,孙培冬,季晓彤,等.黄蜀葵花黄酮抗氧化性及对DNA氧化损伤的保护作用[J].食品与机械,2017,33(2):135-139.
[15] 许岩磊,陈绪,卞卫和,等.疮灵液调控慢性创面金属蛋白酶表达促进愈合的实验研究[J].辽宁中医药大学学报,2016,18(6):25-28.
[16] 张媞.疮灵液基于解毒收敛改善浆细胞性乳腺炎疮面炎症以促进愈合临床研究[D].南京:南京中医药大学,2017.