消癌平注射液提取浓缩工艺的优化
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摘要
目的采用充氮-纳滤耦合技术优化消癌平注射液的提取浓缩工艺。方法以绿原酸及总酚酸为指标,采用HPLC和紫外可见分光光度法分析两者含有量的变化,比较常规方法与充氮-纳滤耦合技术的提取浓缩效果。结果充氮提取后,绿原酸及总酚酸的含有量分别提高了33.68%和22.22%;纳滤浓缩后,两者分别提高了174.73%和97.42%。结论该技术重复性好,可应用于中药制剂生产过程中的提取和浓缩,尤其是对含有易氧化成分者。
AIM To optimize the extraction and concentration process of Xiaoaiping Injection( an anti-tumor agent) by nitrogen pressurization-nanofiltration coupling technology. METHODS With chlorogenic acid and total phenolic acids as indices,their content changes were determined by HPLC and ultraviolet-visible spectrophotometry. The performances of conventional method and nitrogen pressurization-nanofiltration coupling technology in extraction and concentration were compared. RESULTS The contents of chlorogenic acid and total phenolic acids were increased by 33. 68% and 22. 22% due to the application of pressurized nitrogen,and the increasement reached at 174. 73% and 97. 42% respectively after the successive nanofiltration concentration. CONCLUSION With a good reproducibility,nitrogen pressurization-nanofiltration coupling technology can be used for the extraction and concentration of Chinese herbal preparations,especially for those containing easily-oxidized constituents.
AIM To optimize the extraction and concentration process of Xiaoaiping Injection( an anti-tumor agent) by nitrogen pressurization-nanofiltration coupling technology. METHODS With chlorogenic acid and total phenolic acids as indices,their content changes were determined by HPLC and ultraviolet-visible spectrophotometry. The performances of conventional method and nitrogen pressurization-nanofiltration coupling technology in extraction and concentration were compared. RESULTS The contents of chlorogenic acid and total phenolic acids were increased by 33. 68% and 22. 22% due to the application of pressurized nitrogen,and the increasement reached at 174. 73% and 97. 42% respectively after the successive nanofiltration concentration. CONCLUSION With a good reproducibility,nitrogen pressurization-nanofiltration coupling technology can be used for the extraction and concentration of Chinese herbal preparations,especially for those containing easily-oxidized constituents.
引文
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[8]温瑞卿,李东辉,王鑫.HPLC法和聚类分析法探讨不同厂家消癌平片中绿原酸含量的一致性和稳定性[J].中成药,2011,32(11):2021-2023.
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[2]宋亚玲,王红梅,倪付勇,等.金银花中酚酸类成分及其抗炎活性研究[J].中草药,2015,46(4):490-495.
[3]徐龙泉,彭黔荣,杨敏,等.膜分离技术在中药生产及研究中的应用进展[J].中成药,2013,35(9):1989-1994.
[4]胡立新,崔朝亮,程新华,等.利用膜分离技术对牛初乳进行除菌浓缩的研究[J].食品科技,2007(3):90-92.
[5]牟旭凤,白庆中,陈红盛,等.聚合物辅助超滤/纳滤技术处理模拟放射性废水[J].给水排水,2006,32(S1):174-177.
[6]张丽坤,于海瑞.活性炭和氮气对复方氨基酸注射液生产中色氨酸含量的影响[J].黑龙江医药,2009,22(6):840-842.
[7]李存玉,钱祥,杨泽秋,等.基于纳滤技术的消癌平注射液中钾离子去除工艺优化[J].中成药,2015,37(2):294-297.
[8]温瑞卿,李东辉,王鑫.HPLC法和聚类分析法探讨不同厂家消癌平片中绿原酸含量的一致性和稳定性[J].中成药,2011,32(11):2021-2023.
[9]温瑞卿,李东辉,韩杰,等.消癌平片中绿原酸测定的影响因素和优化研究[J].中成药,2012,34(4):762-764.
[10]付煜荣,张万明,陈桂,等.景天三七中没食子酸和总酚酸含量测定[J].中成药,2009,28(7):1016-1018.