枝状枝孢菌次级代谢产物的研究
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摘要
目的 :研究枝状枝孢菌大米固体发酵后产生的次级代谢产物。方法 :采用硅胶柱色谱、凝胶柱色谱、高效液相色谱等方法进行分离纯化,以及NMR、MS等现代波谱手段进行结构鉴定。结果 :分离得到6个单体化合物,分别鉴定为:6,8-二羟基-3-甲基-1H-异铬-1-酮、6-甲氧基-8-羟基-3-甲基异香豆素、豆甾-4-烯-3-酮、3β-羟基-5α,8α-过氧化麦角甾-6,22-二烯、腺嘌呤核苷、甘露醇。结论 :化合物1～3为首次从该植物内生真菌中分离得到。
Objective :To study the secondary metabolites produced by the solid fermentation of Cladosporium sinensis.Methods :Silica gel column chromatography,gel column chromatography,high performance liquid chromatography and other methods were used for separation and purification,as well as NMR,MS and other modern spectral methods for structural identification.RESULTS :Six monomeric compounds were isolated and identified as :6,8-dihydroxy-3-methyl-1 H-isochrom-1-one(1),6-methoxy-8-hydroxy-3-Methyl isocoumarin(2),myristate-4-en-3-one(3),3β-hydroxy-5α,8α-peroxidized ergot-6,22-diene(4),adenine nucleoside(5),mannitol(6).Conclusion :Compounds 1-3 were isolated from the endophytic fungi of this plant for the first time.
Objective :To study the secondary metabolites produced by the solid fermentation of Cladosporium sinensis.Methods :Silica gel column chromatography,gel column chromatography,high performance liquid chromatography and other methods were used for separation and purification,as well as NMR,MS and other modern spectral methods for structural identification.RESULTS :Six monomeric compounds were isolated and identified as :6,8-dihydroxy-3-methyl-1 H-isochrom-1-one(1),6-methoxy-8-hydroxy-3-Methyl isocoumarin(2),myristate-4-en-3-one(3),3β-hydroxy-5α,8α-peroxidized ergot-6,22-diene(4),adenine nucleoside(5),mannitol(6).Conclusion :Compounds 1-3 were isolated from the endophytic fungi of this plant for the first time.
引文
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[2]李东伟,徐红梅,梅涛,等.云南会泽铅锌矿废弃矿渣堆常见植物内生真菌多样性[J].生态学报,2012,32(7):2288-2293.
[3]Yang Xiao-Long,Zhang Jing-Ze,Luo Du-Qiang.The taxonomy,biology and chemistry of the fungal Pestalotiopsis genus[J].Natural Product Reports,2012,29(6):609-712.
[4]胡从从,成斌,王坤,等.蒙古沙冬青及其半生植物AM真菌物种多样性[J].生态学报,2017,37(23):7972-7982.
[5]Singh,B.,Parshad,R.,Khajuria,R.K.,et al.Saccharonol B,a new cytotoxic methylated isocoumarin from Saccharomonospora azurea[J].Tetrahedron Letters,2013,54:6695-6699.
[6]Gremaud,G.,Tabacchi,R.Isocoumarins of the fungus ceratocystis fimbriata coffea[J].Natural Product Letters,1994,5:95-103.
[7]李小军,袁燕,李芝,等.苗药冠盖藤的化学成分研究[J].中草药,2014,45(8):1052-1055.
[8]Niu,X.M.,Li,S.H.Peng,L.Y.,et al.Constituents from Limonia Crenulata[J].Journal of Asian Natural Products Research,2001,3:299-311.
[9]Domondon Denisa L,He Weidong,De Kimpe Norbert,et al.β-adenosine,a bioactive compound in grass chaff stimulating mushroom production[J].Phytochemistry,2004,65,181-187.
[10]张艳玲,甘茂罗,李帅,等.大叶水团花茎枝的化学成分研究[J].中国中药杂志,2010,35(10):1261-1271.